Detergent composition comprising xanthan lyase and endoglucanase variants

ABSTRACT

The present invention relates to a detergent composition comprising endoglucanase variants and xanthan lyase variants and methods for use of said compositions.

REFERENCE TO A SEQUENCE LISTING This application contains a SequenceListing in computer readable form, which is incorporated herein byreference. BACKGROUND OF THE INVENTION Field of the Invention

The present invention relates to a detergent composition, such aslaundry compositions and dish wash compositions, including hand wash andautomatic dish wash compositions, comprising xanthan lyase andendoglucanase variants exhibiting alterations relative to the respectiveparent xanthan lyase and endoglucanase, respectively, in one or moreproperties including: detergent stability (e.g. improved stability in adetergent composition, e.g. in the presence of a chelator, e.g. EDTA orcitrate) and/or storage stability (e.g. improved storage stability in adetergent composition, e.g. in the presence of a chelator, e.g. EDTA orcitrate). The present invention further relates to detergentcompositions comprising xanthan lyase and endoglucanase variants havingactivity on xanthan gum. The invention also relates to methods forproducing and using the compositions of the invention. The variantsdescribed herein are particularly suitable for use in cleaning processesand detergent compositions.

Description of the Related Art

Xanthan gum is a polysaccharide derived from the bacterial coat ofXanthomonas campestris. It is produced by the fermentation of glucose,sucrose, or lactose by the Xanthomonas campestris bacterium. After afermentation period, the polysaccharide is precipitated from a growthmedium with isopropyl alcohol, dried, and ground into a fine powder.Later, it is added to a liquid medium to form the gum. Xanthan gum is anatural polysaccharide consisting of different sugars which areconnected by several different bonds, such as□-D-mannosyl-□-D-1,4-glucuronosyl bonds and□-D-glucosyl-□-D-1,4-glucosyl bonds. Xanthan gum is at least partlysoluble in water and forms highly viscous solutions or gels. Completeenzymatic degradation of xanthan gum requires several enzymaticactivities including xanthan lyase activity and endo-□-1,4-glucanaseactivity. Xanthan lyases are enzymes that cleave the□-D-mannosyl-□-D-1,4-glucuronosyl bond of xanthan and have beendescribed in the literature. Xanthan lyases are known in the art, e.g.two xanthan lyases have been isolated from Paenibacillus alginolyticusXL-1 (e.g. Ruijssenaars et al. (1999) ‘A pyruvated mannose-specificxanthan lyase involved in xanthan degradation by Paenibacillusalginolyticus XL-1’, Appl. Environ. Microbiol. 65(6): 2446-2452, andRuijssenaars et al. (2000), ‘A novel gene encoding xanthan lyase ofPaenibacillus alginolyticus strain XL-1’, Appl. Environ. Microbiol.66(9): 3945-3950). Glycoside hydrolases are enzymes that catalyse thehydrolysis of the glycosyl bond to release smaller sugars. There areover 100 classes of glycoside hydrolases which have been classified, seeHenrissat et al. (1991) ‘A classification of glycosyl hydrolases basedon amino-acid sequence similarities’, J. Biochem. 280: 309-316 and theUniprot website at www.cazy.org. The glycoside hydrolase family 9 (GH9)consists of over 70 different enzymes that are mostly endo-glucanases(EC 3.2.1.4), cellobiohydrolases (EC 3.2.1.91), β-glucosidases (EC3.2.1.21) and exo-6-glucosaminidase (EC 3.2.1.165). In recent years,xanthan gum has been used as an ingredient in many consumer productsincluding foods (e.g. as thickening agent in salad dressings and dairyproducts) and cosmetics (e.g. as stabilizer and thickener in toothpasteand make-up, creams and lotions to prevent ingredients from separatingand to provide the right texture of the product). Further xanthan gumhas found use in the oil industry as an additive to regulate theviscosity of drilling fluids etc. The widespread use of xanthan gum hasled to a desire to degrade solutions, gels or mixtures containingxanthan gum thereby allowing easier removal of the byproducts.Endoglucanases and xanthan lyases for the degradation of xanthan gum andthe use of such enzymes for cleaning purposes, such as the removal ofxanthan gum containing stains, and in the drilling and oil industriesare known in the art, e.g. WO2013/167581A1.

The known xanthan endoglucanase having SEQ ID NO:2 and the known xanthanlyase having SEQ ID NO:6 were both found to be sensitive to the presenceof detergents with chelators. To improve the applicability and/or costand/or the performance of such enzymes, there is an ongoing search forvariants with altered properties, such as increased stability, e.g.improved stability in a detergent composition, e.g. in the presence of achelator, e.g. EDTA or citrate, etc. However, mutagenesis of largeenzymes followed by purification and functional analysis of mutantlibraries can be very expensive and laborious.

SUMMARY OF THE INVENTION

In some aspects, the present invention relates to a detergentcomposition comprising

(A) an endoglucanase variant, comprising an alteration (e.g. asubstitution, deletion or insertion, preferably a substitution) at oneor more positions in a (chelator-induced instability) region selectedfrom the group consisting of: region 1 corresponding to amino acids 95to 105 of SEQ ID NO:2, region 2 corresponding to amino acids 115 to 138of SEQ ID NO:2, region 3 corresponding to amino acids 210 to 251 of SEQID NO:2, region 4 corresponding to amino acids 267 to 301 of SEQ IDNO:2, region 5 corresponding to amino acids 339 to 361 of SEQ ID NO:2,region 6 corresponding to amino acids 547 to 595 of SEQ ID NO:2, region7 corresponding to amino acids 612 to 660 of SEQ ID NO:2, region 8corresponding to amino acids 806 to 828 of SEQ ID NO:2, region 9corresponding to amino acids 839 to 1042 of SEQ ID NO:2, and/or region10 corresponding to amino acids 1 to 94 of SEQ ID NO:2, region 11corresponding to amino acids 106 to 114 of SEQ ID NO:2, region 12corresponding to amino acids 139 to 209 of SEQ ID NO:2, region 13corresponding to amino acids 252 to 266 of SEQ ID NO:2, region 14corresponding to amino acids 302 to 338 of SEQ ID NO:2, region 15corresponding to amino acids 362 to 546 of SEQ ID NO:2, region 16corresponding to amino acids 596 to 611 of SEQ ID NO:2, region 17corresponding to amino acids 661 to 805 of SEQ ID NO:2, region 18corresponding to amino acids 829 to 838 of SEQ ID NO:2, and region 19corresponding to amino acids 1043 to 1055 of SEQ ID NO:2; and

(B) a xanthan lyase variant, comprising an alteration (e.g. asubstitution, deletion or insertion, preferably a substitution) at oneor more positions in a (chelator-induced instability) region selectedfrom the group consisting of: region 1 corresponding to amino acids 154to 176 of SEQ ID NO:6, region 2 corresponding to amino acids 614 to 658of SEQ ID NO:6, region 3 corresponding to amino acids 731 to 803 of SEQID NO:6, region 4 corresponding to amino acids 807 to 846 of SEQ IDNO:6, region 5 corresponding to amino acids 872 to 885 of SEQ ID NO:6,region 6 corresponding to amino acids 903 to 1004 of SEQ ID NO:6, and/orregion 7 corresponding to amino acids 1 to 153 of SEQ ID NO:6, region 8corresponding to amino acids 177 to 613 of SEQ ID NO:6, region 9corresponding to amino acids 659 to 730 of SEQ ID NO:6, region 10corresponding to amino acids 804 to 806 of SEQ ID NO:6, region 11corresponding to amino acids 847 to 871 of SEQ ID NO:6, region 12corresponding to amino acids 886 to 902 of SEQ ID NO:6, and region 13corresponding to amino acids 1005 to 1037 of SEQ ID NO:6.

In various aspects, the endoglucanase variant (A) has at least 60% andless than 100% sequence identity to SEQ ID NO:2; preferably saidendoglucanase variant has activity on xanthan gum pre-treated withxanthan lyase; and/or the xanthan lyase variant (B) has at least 60% andless than 100% sequence identity to SEQ ID NO:6 preferably said xanthanlyase variant having an activity on xanthan gum.

In some aspects, the present invention defines a chelator-inducedinstability region of a parent endoglucanase (e.g. SEQ ID NO:2) or aparent xanthan lyase (e.g. SEQ ID NO:6) having one or more of thefollowing features: in the presence of a chelator it is relatively lessconformationally stable than one or more or all of its adjacent regions;and/or in the presence of a chelator it is relatively more exposed tosaid chelator than one or more or all of its adjacent regions; and/or inthe presence of a chelator it is relatively more accessible to saidchelator than one or more or all of its adjacent regions; and/or in thepresence of a chelator it is relatively more conformationally dynamicthan one or more or all of its adjacent regions; and/or in the presenceof a chelator it is relatively more receptive to deuterium incorporationthan one or more or all of its adjacent regions. In the presentinvention, when referring to “relatively” it means that the features ofa given region as indicated above are based on observed differencesbetween its features and/or properties in the presence of a chelator andits features and/or properties in the absence of a chelator (i.e. inorder to determine the impact of a chelator, each region is compared toitself in the absence of said chelator and any changes are determinedrelative to its native features/properties in the absence of achelator).

In some aspects, the present invention relates to a detergentcomposition, as defined herein, comprising an endoglucanase varianthaving at least 61%, 62%, 63%, 64%, 65%, 66%, 67%, 68%, 69%, 70%, 71%,72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%,86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, or 99%sequence identity to SEQ ID NO:2.

In some aspects, the present invention relates to a detergentcomposition, as defined herein, comprising a xanthan lyase varianthaving at least 61%, 62%, 63%, 64%, 65%, 66%, 67%, 68%, 69%, 70%, 71%,72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%,86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, or 99%sequence identity to SEQ ID NO:6.

In some aspects, the detergent composition comprises an endoglucanasevariant, comprising an alteration (e.g. a substitution, deletion orinsertion) at one or more positions in a region selected from the groupconsisting of:

i) region 1 corresponding to amino acids 95 to 105 of SEQ ID NO:2, e.g.said alteration at one or more positions selected from the groupconsisting of positions: 95, 96, 97, 98, 99, 100, 101, 102, 103, 104,105, wherein said positions correspond to amino acid positions of SEQ IDNO:2 (e.g. using the numbering of SEQ ID NO:2),

ii) region 2 corresponding to amino acids 115 to 138 of SEQ ID NO:2,e.g. said alteration at one or more positions selected from the groupconsisting of positions: 115, 116, 117, 118, 119, 120, 121, 122, 123,124, 125, 126, 127, 128, 129, 130, 131, 132, 133, 134, 135, 136, 137,138, wherein said positions correspond to amino acid positions of SEQ IDNO:2 (e.g. using the numbering of SEQ ID NO:2),

iii) region 3 corresponding to amino acids 210 to 251 of SEQ ID NO:2,e.g. said alteration at one or more positions selected from the groupconsisting of positions: 210, 211, 212, 213, 214, 215, 216, 217, 218,219, 220, 221, 222, 223, 224, 225, 226, 227, 228, 229, 230, 231, 232,233, 234, 235, 236, 237, 238, 239, 240, 241, 242, 243, 244, 245, 246,247, 248, 249, 250, 251, wherein said positions correspond to amino acidpositions of SEQ ID NO:2 (e.g. using the numbering of SEQ ID NO:2),

iv) region 4 corresponding to amino acids 267 to 301 of SEQ ID NO:2,e.g. said alteration at one or more positions selected from the groupconsisting of positions: 267, 268, 269, 270, 271, 272, 273, 274, 275,276, 277, 278, 279, 280, 281, 282, 283, 284, 285, 286, 287, 288, 289,290, 291, 292, 293, 294, 295, 296, 297, 298, 299, 300, 301, wherein saidpositions correspond to amino acid positions of SEQ ID NO:2 (e.g. usingthe numbering of SEQ ID NO:2),

v) region 5 corresponding to amino acids 339 to 361 of SEQ ID NO:2, e.g.said alteration at one or more positions selected from the groupconsisting of positions: 339, 340, 341, 342, 343, 344, 345, 346, 347,348, 349, 350, 351, 352, 353, 354, 355, 356, 357, 358, 359, 360, 361,wherein said positions correspond to amino acid positions of SEQ ID NO:2(e.g. using the numbering of SEQ ID NO:2),

vi) region 6 corresponding to amino acids 547 to 595 of SEQ ID NO:2,e.g. said alteration at one or more positions selected from the groupconsisting of positions: 547, 548, 549, 550, 551, 552, 553, 554, 555,556, 557, 558, 559, 560, 561, 562, 563, 564, 565, 566, 567, 568, 569,570, 571, 572, 573, 574, 575, 576, 577, 578, 579, 580, 581, 582, 583,584, 585, 586, 587, 588, 589, 590, 591, 592, 593, 594, 595, wherein saidpositions correspond to amino acid positions of SEQ ID NO:2 (e.g. usingthe numbering of SEQ ID NO:2),

vii) region 7 corresponding to amino acids 612 to 660 of SEQ ID NO:2,e.g. said alteration at one or more positions selected from the groupconsisting of positions: 612, 613, 614, 615, 616, 617, 618, 619, 620,621, 622, 623, 624, 625, 626, 627, 628, 629, 630, 631, 632, 633, 634,635, 636, 637, 638, 639, 640, 641, 642, 643, 644, 645, 646, 647, 648,649, 650, 651, 652, 653, 654, 655, 656, 657, 658, 659, 660, wherein saidpositions correspond to amino acid positions of SEQ ID NO:2 (e.g. usingthe numbering of SEQ ID NO:2),

viii) region 8 corresponding to amino acids 806 to 828 of SEQ ID NO:2,e.g. said alteration at one or more positions selected from the groupconsisting of positions: 806, 807, 808, 809, 810, 811, 812, 813, 814,815, 816, 817, 818, 819, 820, 821, 822, 823, 824, 825, 826, 827, 828,wherein said positions correspond to amino acid positions of SEQ ID NO:2(e.g. using the numbering of SEQ ID NO:2),

ix) region 9 corresponding to amino acids 839 to 1042 of SEQ ID NO:2,e.g. said alteration at one or more positions selected from the groupconsisting of positions: 839, 840, 841, 842, 843, 844, 845, 846, 847,848, 849, 850, 851, 852, 853, 854, 855, 856, 857, 858, 859, 860, 861,862, 863, 864, 865, 866, 867, 868, 869, 870, 871, 872, 873, 874, 875,876, 877, 878, 879, 880, 881, 882, 883, 884, 885, 886, 887, 888, 889,890, 891, 892, 893, 894, 895, 896, 897, 898, 899, 900, 901, 902, 903,904, 905, 906, 907, 908, 909, 910, 911, 912, 913, 914, 915, 916, 917,918, 919, 920, 921, 922, 923, 924, 925, 926, 927, 928, 929, 930, 931,932, 933, 934, 935, 936, 937, 938, 939, 940, 941, 942, 943, 944, 945,946, 947, 948, 949, 950, 951, 952, 953, 954, 955, 956, 957, 958, 959,960, 961, 962, 963, 964, 965, 966, 967, 968, 969, 970, 971, 972, 973,974, 975, 976, 977, 978, 979, 980, 981, 982, 983, 984, 985, 986, 987,988, 989, 990, 991, 992, 993, 994, 995, 996, 997, 998, 999, 1000, 1001,1002, 1003, 1004, 1005, 1006, 1007, 1008, 1009, 1010, 1011, 1012, 1013,1014, 1015, 1016, 1017, 1018, 1019, 1020, 1021, 1022, 1023, 1024, 1025,1026, 1027, 1028, 1029, 1030, 1031, 1032, 1033, 1034, 1035, 1036, 1037,1038, 1039, 1040, 1041, 1042, wherein said positions correspond to aminoacid positions of SEQ ID NO:2 (e.g. using the numbering of SEQ ID NO:2),

x) region 10 corresponding to amino acids 1 to 94 of SEQ ID NO:2, e.g.said alteration at one or more positions selected from the groupconsisting of positions: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14,15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32,33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50,51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68,69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86,87, 88, 89, 90, 91, 92, 93, 94, wherein said positions correspond toamino acid positions of SEQ ID NO:2 (e.g. using the numbering of SEQ IDNO:2),

xi) region 11 corresponding to amino acids 106 to 114 of SEQ ID NO:2,e.g. said alteration at one or more positions selected from the groupconsisting of positions: 107, 108, 109, 110, 111, 112, 113, 114, whereinsaid positions correspond to amino acid positions of SEQ ID NO:2 (e.g.using the numbering of SEQ ID NO:2),

xii) region 12 corresponding to amino acids 139 to 209 of SEQ ID NO:2,e.g. said alteration at one or more positions selected from the groupconsisting of positions: 139, 140, 141, 142, 143, 144, 145, 146, 147,148, 149, 150, 151, 152, 153, 154, 155, 156, 157, 158, 159, 160, 161,162, 163, 164, 165, 166, 167, 168, 169, 170, 171, 172, 173, 174, 175,176, 177, 178, 179, 180, 181, 182, 183, 184, 185, 186, 187, 188, 189,190, 191, 192, 193, 194, 195, 196, 197, 198, 199, 200, 201, 202, 203,204, 205, 206, 207, 208, 209, wherein said positions correspond to aminoacid positions of SEQ ID NO:2 (e.g. using the numbering of SEQ ID NO:2),

xiii) region 13 corresponding to amino acids 252 to 266 of SEQ ID NO:2,e.g. said alteration at one or more positions selected from the groupconsisting of positions: 252, 253, 254, 255, 256, 257, 258, 259, 260,261, 262, 263, 264, 265, 266, wherein said positions correspond to aminoacid positions of SEQ ID NO:2 (e.g. using the numbering of SEQ ID NO:2),

xiv) region 14 corresponding to amino acids 302 to 338 of SEQ ID NO:2,e.g. said alteration at one or more positions selected from the groupconsisting of positions: 302, 303, 304, 305, 306, 307, 308, 309, 310,311, 312, 313, 314, 315, 316, 317, 318, 319, 320, 321, 322, 323, 324,325, 326, 327, 328, 329, 330, 331, 332, 333, 334, 335, 336, 337, 338,wherein said positions correspond to amino acid positions of SEQ ID NO:2(e.g. using the numbering of SEQ ID NO:2),

xv) region 15 corresponding to amino acids 362 to 546 of SEQ ID NO:2,e.g. said alteration at one or more positions selected from the groupconsisting of positions: 362, 363, 364, 365, 366, 367, 368, 369, 370,371, 372, 373, 374, 375, 376, 377, 378, 379, 380, 381, 382, 383, 384,385, 386, 387, 388, 389, 390, 391, 392, 393, 394, 395, 396, 397, 398,399, 400, 401, 402, 403, 404, 405, 406, 407, 408, 409, 410, 411, 412,413, 414, 415, 416, 417, 418, 419, 420, 421, 422, 423, 424, 425, 426,427, 428, 429, 430, 431, 432, 433, 434, 435, 436, 437, 438, 439, 440,441, 442, 443, 444, 445, 446, 447, 448, 449, 450, 451, 452, 453, 454,455, 456, 457, 458, 459, 460, 461, 462, 463, 464, 465, 466, 467, 468,469, 470, 471, 472, 473, 474, 475, 476, 477, 478, 479, 480, 481, 482,483, 484, 485, 486, 487, 488, 489, 490, 491, 492, 493, 494, 495, 496,497, 498, 499, 500, 501, 502, 503, 504, 505, 506, 507, 508, 509, 510,511, 512, 513, 514, 515, 516, 517, 518, 519, 520, 521, 522, 523, 524,525, 526, 527, 528, 529, 530, 531, 532, 533, 534, 535, 536, 537, 538,539, 540, 541, 542, 543, 544, 545, 546, wherein said positionscorrespond to amino acid positions of SEQ ID NO:2 (e.g. using thenumbering of SEQ ID NO:2),

xvi) region 16 corresponding to amino acids 596 to 611 of SEQ ID NO:2,e.g. said alteration at one or more positions selected from the groupconsisting of positions: 596, 597, 598, 599, 600, 601, 602, 603, 604,605, 606, 607, 608, 609, 610, 611, wherein said positions correspond toamino acid positions of SEQ ID NO:2 (e.g. using the numbering of SEQ IDNO:2),

xvii) region 17 corresponding to amino acids 661 to 805 of SEQ ID NO:2,e.g. said alteration at one or more positions selected from the groupconsisting of positions: 661, 662, 663, 664, 665, 666, 667, 668, 669,670, 671, 672, 673, 674, 675, 676, 677, 678, 679, 680, 681, 682, 683,684, 685, 686, 687, 688, 689, 690, 691, 692, 693, 694, 695, 696, 697,698, 699, 700, 701, 702, 703, 704, 705, 706, 707, 708, 709, 710, 711,712, 713, 714, 715, 716, 717, 718, 719, 720, 721, 722, 723, 724, 725,726, 727, 728, 729, 730, 731, 732, 733, 734, 735, 736, 737, 738, 739,740, 741, 742, 743, 744, 745, 746, 747, 748, 749, 750, 751, 752, 753,754, 755, 756, 757, 758, 759, 760, 761, 762, 763, 764, 765, 766, 767,768, 769, 770, 771, 772, 773, 774, 775, 776, 777, 778, 779, 780, , 781,782, 783, 784, 785, 786, 787, 788, 789, 790, 791, 792, 793, 794, 795,796, 797, 798, 799, 800, 801, 802, 803, 804, 805, wherein said positionscorrespond to amino acid positions of SEQ ID NO:2 (e.g. using thenumbering of SEQ ID NO:2),

xviii) region 18 corresponding to amino acids 829 to 838 to 1042 of SEQID NO:2, e.g. said alteration at one or more positions selected from thegroup consisting of positions: 829, 830, 831, 832, 833, 834, 835, 836,837, 838, wherein said positions correspond to amino acid positions ofSEQ ID NO:2 (e.g. using the numbering of SEQ ID NO:2), and xix) region19 corresponding to amino acids 1043 to 1055 of SEQ ID NO:2, e.g. saidalteration at one or more positions selected from the group consistingof positions 1043, 1044, 1045, 1046, 1047, 1048, 1049, 1050, 1051, 1052,1053, 1054, 1055, wherein said positions correspond to amino acidpositions of SEQ ID NO:2 (e.g. using the numbering of SEQ ID NO:2).

In some aspects, the afore-mentioned variants have at least 60% and lessthan 100% sequence identity to SEQ ID NO:2, preferably saidendoglucanase variant having activity on xanthan gum pre-treated withxanthan lyase.

In some aspects, the detergent composition comprises a xanthan lyasevariant comprising an alteration (e.g. a substitution, deletion orinsertion, preferably a substitution) at one or more positions in aregion selected from the group consisting of:

i) region 1 corresponding to amino acids 154 to 176 of SEQ ID NO:6, e.g.said alteration at one or more positions selected from the groupconsisting of positions: 154, 155, 156, 157, 158, 159, 160, 161, 162,163, 164, 165, 166, 167, 168, 169, 170, 171, 172, 173, 174, 175, 176,wherein said positions correspond to amino acid positions of SEQ ID NO:6(e.g. using the numbering of SEQ ID NO:6),

ii) region 2 corresponding to amino acids 614 to 658 of SEQ ID NO:6,e.g. said alteration at one or more positions selected from the groupconsisting of positions: 614, 615, 616, 617, 618, 619, 620, 621, 622,623, 624, 625, 626, 627, 628, 629, 630, 631, 632, 633, 634, 635, 636,637, 638, 639, 640, 641, 642, 643, 644, 645, 646, 647, 648, 649, 650,651, 652, 653, 654, 655, 656, 657, 658, wherein said positionscorrespond to amino acid positions of SEQ ID NO:6 (e.g. using thenumbering of SEQ ID NO:6),

iii) region 3 corresponding to amino acids 731 to 803 of SEQ ID NO:6,e.g. said alteration at one or more positions selected from the groupconsisting of positions: 731, 732, 733, 734, 735, 736, 737, 738, 739,740, 741, 742, 743, 744, 745, 746, 747, 748, 749, 750, 751, 752, 753,754, 755, 756, 757, 758, 759, 760, 761, 762, 763, 764, 765, 766, 767,768, 769, 770, 771, 772, 773, 774, 775, 776, 777, 778, 779, 780, , 781,782, 783, 784, 785, 786, 787, 788, 789, 790, 791, 792, 793, 794, 795,796, 797, 798, 799, 800, 801, 802, 803, wherein said positionscorrespond to amino acid positions of SEQ ID NO:6 (e.g. using thenumbering of SEQ ID NO:6),

iv) region 4 corresponding to amino acids 807 to 846 of SEQ ID NO:6,e.g. said alteration at one or more positions selected from the groupconsisting of positions: 807, 808, 809, 810, 811, 812, 813, 814, 815,816, 817, 818, 819, 820, 821, 822, 823, 824, 825, 826, 827, 828, 829,830, 831, 832, 833, 834, 835, 836, 837, 838, 839, 840, 841, 842, 843,844, 845, 846, wherein said positions correspond to amino acid positionsof SEQ ID NO:6 (e.g. using the numbering of SEQ ID NO:6),

v) region 5 corresponding to amino acids 872 to 885 of SEQ ID NO:6, e.g.said alteration at one or more positions selected from the groupconsisting of positions: 872, 873, 874, 875, 876, 877, 878, 879, 880,881, 882, 883, 884, 885, wherein said positions correspond to amino acidpositions of SEQ ID NO:6 (e.g. using the numbering of SEQ ID NO:6),

vi) region 6 corresponding to amino acids 903 to 1004 of SEQ ID NO:6,e.g. said alteration at one or more positions selected from the groupconsisting of positions: 903, 904, 905, 906, 907, 908, 909, 910, 911,912, 913, 914, 915, 916, 917, 918, 919, 920, 921, 922, 923, 924, 925,926, 927, 928, 929, 930, 931, 932, 933, 934, 935, 936, 937, 938, 939,940, 941, 942, 943, 944, 945, 946, 947, 948, 949, 950, 951, 952, 953,954, 955, 956, 957, 958, 959, 960, 961, 962, 963, 964, 965, 966, 967,968, 969, 970, 971, 972, 973, 974, 975, 976, 977, 978, 979, 980, 981,982, 983, 984, 985, 986, 987, 988, 989, 990, 991, 992, 993, 994, 995,996, 997, 998, 999, 1000, 1001, 1002, 1003, 1004, wherein said positionscorrespond to amino acid positions of SEQ ID NO:6 (e.g. using thenumbering of SEQ ID NO:6), 6p vii) region 7 corresponding to amino acids1 to 153 of SEQ ID NO:6, e.g. said alteration at one or more positionsselected from the group consisting of positions: 1, 2, 3, 4, 5, 6, 7, 8,9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26,27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44,45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62,63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80,81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98,99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112,113, 114, 115, 116, 117, 118, 119, 120, 121, 122, 123, 124, 125, 126,127, 128, 129, 130, 131, 132, 133, 134, 135, 136, 137, 138, 139, 140,141, 142, 143, 144, 145, 146, 147, 148, 149, 150, 151, 152 and 153,wherein said positions correspond to amino acid positions of SEQ IDNO:6,

viii) region 8 corresponding to amino acids 177 to 613 of SEQ ID NO:6,e.g. said alteration at one or more positions selected from the groupconsisting of positions: 177, 178, 179, 180, 181, 182, 183, 184, 185,186, 187, 188, 189, 190, 191, 192, 193, 194, 195, 196, 197, 198, 199,200, 201, 202, 203, 204, 205, 206, 207, 208, 209, 210, 211, 212, 213,214, 215, 216, 217, 218, 219, 220, 221, 222, 223, 224, 225, 226, 227,228, 229, 230, 231, 232, 233, 234, 235, 236, 237, 238, 239, 240, 241,242, 243, 244, 245, 246, 247, 248, 249, 250, 251, 252, 253, 254, 255,256, 257, 258, 259, 260, 261, 262, 263, 264, 265, 266, 267, 268, 269,270, 271, 272, 273, 274, 275, 276, 277, 278, 279, 280, 281, 282, 283,284, 285, 286, 287, 288, 289, 290, 291, 292, 293, 294, 295, 296, 297,298, 299, 300, 301, 302, 303, 304, 305, 306, 307, 308, 309, 310, 311,312, 313, 314, 315, 316, 317, 318, 319, 320, 321, 322, 323, 324, 325,326, 327, 328, 329, 330, 331, 332, 333, 334, 335, 336, 337, 338, 339,340, 341, 342, 343, 344, 345, 346, 347, 348, 349, 350, 351, 352, 353,354, 355, 356, 357, 358, 359, 360, 361, 362, 363, 364, 365, 366, 367,368, 369, 370, 371, 372, 373, 374, 375, 376, 377, 378, 379, 380, 381,382, 383, 384, 385, 386, 387, 388, 389, 390, 391, 392, 393, 394, 395,396, 397, 398, 399, 400, 401, 402, 403, 404, 405, 406, 407, 408, 409,410, 411, 412, 413, 414, 415, 416, 417, 418, 419, 420, 421, 422, 423,424, 425, 426, 427, 428, 429, 430, 431, 432, 433, 434, 435, 436, 437,438, 439, 440, 441, 442, 443, 444, 445, 446, 447, 448, 449, 450, 451,452, 453, 454, 455, 456, 457, 458, 459, 460, 461, 462, 463, 464, 465,466, 467, 468, 469, 470, 471, 472, 473, 474, 475, 476, 477, 478, 479,480, 481, 482, 483, 484, 485, 486, 487, 488, 489, 490, 491, 492, 493,494, 495, 496, 497, 498, 499, 500, 501, 502, 503, 504, 505, 506, 507,508, 509, 510, 511, 512, 513, 514, 515, 516, 517, 518, 519, 520, 521,522, 523, 524, 525, 526, 527, 528, 529, 530, 531, 532, 533, 534, 535,536, 537, 538, 539, 540, 541, 542, 543, 544, 545, 546, 547, 548, 549,550, 551, 552, 553, 554, 555, 556, 557, 558, 559, 560, 561, 562, 563,564, 565, 566, 567, 568, 569, 570, 571, 572, 573, 574, 575, 576, 577,578, 579, 580, 581, 582, 583, 584, 585, 586, 587, 588, 589, 590, 591,592, 593, 594, 595, 596, 597, 598, 599, 600, 601, 602, 603, 604, 605,606, 607, 608, 609, 610, 611, 612 and 613, wherein said positionscorrespond to amino acid positions of SEQ ID NO:6,

ix) region 9 corresponding to amino acids 659 to 730 of SEQ ID NO:6,e.g. said alteration at one or more positions selected from the groupconsisting of positions: 659, 660, 661, 662, 663, 664, 665, 666, 667,668, 669, 670, 671, 672, 673, 674, 675, 676, 677, 678, 679, 680, 681,682, 683, 684, 685, 686, 687, 688, 689, 690, 691, 692, 693, 694, 695,696, 697, 698, 699, 700, 701, 702, 703, 704, 705, 706, 707, 708, 709,710, 711, 712, 713, 714, 715, 716, 717, 718, 719, 720, 721, 722, 723,724, 725, 726, 727, 728, 729 and 730, wherein said positions correspondto amino acid positions of SEQ ID NO:6,

x) region 10 corresponding to amino acids 804 to 806 of SEQ ID NO:6,e.g. said alteration at one or more positions selected from the groupconsisting of positions: 804, 805 and 806, wherein said positionscorrespond to amino acid positions of SEQ ID NO:6,

xi) region 11 corresponding to amino acids 847 to 871 of SEQ ID NO:6,e.g. said alteration at one or more positions selected from the groupconsisting of positions: 847, 848, 849, 850, 851, 852, 853, 854, 855,856, 857, 858, 859, 860, 861, 862, 863, 864, 865, 866, 867, 868, 869,870 and 871, wherein said positions correspond to amino acid positionsof SEQ ID NO:6,

xii) region 12 corresponding to amino acids 886 to 902 of SEQ ID NO:6,e.g. said alteration at one or more positions selected from the groupconsisting of positions: 886, 887, 888, 889, 890, 891, 892, 893, 894,895, 896, 897, 898, 899, 900, 901 and 902, wherein said positionscorrespond to amino acid positions of SEQ ID NO:6, and

xiii) region 13 corresponding to amino acids 1005 to 1037 of SEQ IDNO:6, e.g. said alteration at one or more positions selected from thegroup consisting of positions: 1005, 1006, 1007, 1008, 1009, 1010, 1011,1012, 1013, 1014, 1015, 1016, 1017, 1018, 1019, 1020, 1021, 1022, 1023,1024, 1025, 1026, 1027, 1028, 1029, 1030, 1031, 1032, 1033, 1034, 1035,1036 and 1037, wherein said positions correspond to amino acid positionsof SEQ ID NO:6.

In some aspects, the afore-mentioned variants have at least 60% and lessthan 100% sequence identity to SEQ ID NO:6, preferably said xanthanlyase variant having an activity on xanthan gum.

In some aspects, the detergent composition comprises an endoglucanasevariant, comprising an alteration (e.g. a substitution, deletion orinsertion, preferably a substitution) at one or more positions in:

a) one or more regions selected from the group consisting of:

i) region 1 corresponding to amino acids 95 to 105 of SEQ ID NO:2, e.g.said alteration at one or more positions selected from the groupconsisting of positions: 95, 96, 97, 98, 99, 100, 101, 102, 103, 104,105, wherein said positions correspond to amino acid positions of SEQ IDNO:2 (e.g. using the numbering of SEQ ID NO:2),

ii) region 2 corresponding to amino acids 115 to 138 of SEQ ID NO:2,e.g. said alteration at one or more positions selected from the groupconsisting of positions: 115, 116, 117, 118, 119, 120, 121, 122, 123,124, 125, 126, 127, 128, 129, 130, 131, 132, 133, 134, 135, 136, 137,138, wherein said positions correspond to amino acid positions of SEQ IDNO:2 (e.g. using the numbering of SEQ ID NO:2),

iii) region 3 corresponding to amino acids 210 to 251 of SEQ ID NO:2,e.g. said alteration at one or more positions selected from the groupconsisting of positions: 210, 211, 212, 213, 214, 215, 216, 217, 218,219, 220, 221, 222, 223, 224, 225, 226, 227, 228, 229, 230, 231, 232,233, 234, 235, 236, 237, 238, 239, 240, 241, 242, 243, 244, 245, 246,247, 248, 249, 250, 251, wherein said positions correspond to amino acidpositions of SEQ ID NO:2 (e.g. using the numbering of SEQ ID NO:2),

iv) region 4 corresponding to amino acids 267 to 301 of SEQ ID NO:2,e.g. said alteration at one or more positions selected from the groupconsisting of positions: 267, 268, 269, 270, 271, 272, 273, 274, 275,276, 277, 278, 279, 280, 281, 282, 283, 284, 285, 286, 287, 288, 289,290, 291, 292, 293, 294, 295, 296, 297, 298, 299, 300, 301, wherein saidpositions correspond to amino acid positions of SEQ ID NO:2 (e.g. usingthe numbering of SEQ ID NO:2),

v) region 5 corresponding to amino acids 339 to 361 of SEQ ID NO:2, e.g.said alteration at one or more positions selected from the groupconsisting of positions: 339, 340, 341, 342, 343, 344, 345, 346, 347,348, 349, 350, 351, 352, 353, 354, 355, 356, 357, 358, 359, 360, 361,wherein said positions correspond to amino acid positions of SEQ ID NO:2(e.g. using the numbering of SEQ ID NO:2),

vi) region 6 corresponding to amino acids 547 to 595 of SEQ ID NO:2,e.g. said alteration at one or more positions selected from the groupconsisting of positions: 547, 548, 549, 550, 551, 552, 553, 554, 555,556, 557, 558, 559, 560, 561, 562, 563, 564, 565, 566, 567, 568, 569,570, 571, 572, 573, 574, 575, 576, 577, 578, 579, 580, 581, 582, 583,584, 585, 586, 587, 588, 589, 590, 591, 592, 593, 594, 595, wherein saidpositions correspond to amino acid positions of SEQ ID NO:2 (e.g. usingthe numbering of SEQ ID NO:2),

vii) region 7 corresponding to amino acids 612 to 660 of SEQ ID NO:2,e.g. said alteration at one or more positions selected from the groupconsisting of positions: 612, 613, 614, 615, 616, 617, 618, 619, 620,621, 622, 623, 624, 625, 626, 627, 628, 629, 630, 631, 632, 633, 634,635, 636, 637, 638, 639, 640, 641, 642, 643, 644, 645, 646, 647, 648,649, 650, 651, 652, 653, 654, 655, 656, 657, 658, 659, 660, wherein saidpositions correspond to amino acid positions of SEQ ID NO:2 (e.g. usingthe numbering of SEQ ID NO:2),

viii) region 8 corresponding to amino acids 806 to 828 of SEQ ID NO:2,e.g. said alteration at one or more positions selected from the groupconsisting of positions: 806, 807, 808, 809, 810, 811, 812, 813, 814,815, 816, 817, 818, 819, 820, 821, 822, 823, 824, 825, 826, 827, 828,wherein said positions correspond to amino acid positions of SEQ ID NO:2(e.g. using the numbering of SEQ ID NO:2), and

ix) region 9 corresponding to amino acids 839 to 1042 of SEQ ID NO:2,e.g. said alteration at one or more positions selected from the groupconsisting of positions: 839, 840, 841, 842, 843, 844, 845, 846, 847,848, 849, 850, 851, 852, 853, 854, 855, 856, 857, 858, 859, 860, 861,862, 863, 864, 865, 866, 867, 868, 869, 870, 871, 872, 873, 874, 875,876, 877, 878, 879, 880, 881, 882, 883, 884, 885, 886, 887, 888, 889,890, 891, 892, 893, 894, 895, 896, 897, 898, 899, 900, 901, 902, 903,904, 905, 906, 907, 908, 909, 910, 911, 912, 913, 914, 915, 916, 917,918, 919, 920, 921, 922, 923, 924, 925, 926, 927, 928, 929, 930, 931,932, 933, 934, 935, 936, 937, 938, 939, 940, 941, 942, 943, 944, 945,946, 947, 948, 949, 950, 951, 952, 953, 954, 955, 956, 957, 958, 959,960, 961, 962, 963, 964, 965, 966, 967, 968, 969, 970, 971, 972, 973,974, 975, 976, 977, 978, 979, 980, 981, 982, 983, 984, 985, 986, 987,988, 989, 990, 991, 992, 993, 994, 995, 996, 997, 998, 999, 1000, 1001,1002, 1003, 1004, 1005, 1006, 1007, 1008, 1009, 1010, 1011, 1012, 1013,1014, 1015, 1016, 1017, 1018, 1019, 1020, 1021, 1022, 1023, 1024, 1025,1026, 1027, 1028, 1029, 1030, 1031, 1032, 1033, 1034, 1035, 1036, 1037,1038, 1039, 1040, 1041, 1042, wherein said positions correspond to aminoacid positions of SEQ ID NO:2 (e.g. using the numbering of SEQ ID NO:2);and/or

b) one or more adjacent regions, said adjacent region being selectedfrom the group consisting of: (i′) region 10 corresponding to aminoacids 1 to 94 of SEQ ID NO:2; (ii') region 11 corresponding to aminoacids 106 to 114 of SEQ ID NO:2; (iii′) region 12 corresponding to aminoacids 139 to 209 of SEQ ID NO:2; (iv′) region 13 corresponding to aminoacids 252 to 266 of SEQ ID NO:2; (v′) region 14 corresponding to aminoacids 302 to 338 of SEQ ID NO:2; (vi′) region 15 corresponding to aminoacids 362 to 546 of SEQ ID NO:2; (vii') region 16 corresponding to aminoacids 596 to 611 of SEQ ID NO:2; (viii′) region 17 corresponding toamino acids 661 to 805 of SEQ ID NO:2; (ix′) region 18 corresponding toamino acids 829 to 838 of SEQ ID NO:2; and (x′) region 19 correspondingto amino acids 1043 to 1055 of SEQ ID NO:2.

In some aspects, the present invention relates to a detergentcomposition comprising an endoglucanase variant, comprising analteration (e.g. a substitution, deletion or insertion, preferably asubstitution) at one or more positions in:

a) one or more regions selected from the group consisting of:

i) region 10 corresponding to amino acids 1 to 94 of SEQ ID NO:2, e.g.said alteration at one or more positions selected from the groupconsisting of positions: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14,15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32,33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50,51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68,69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86,87, 88, 89, 90, 91, 92, 93, 94, wherein said positions correspond toamino acid positions of SEQ ID NO:2 (e.g. using the numbering of SEQ IDNO:2),

ii) region 11 corresponding to amino acids 106 to 114 of SEQ ID NO:2,e.g. said alteration at one or more positions selected from the groupconsisting of positions: 107, 108, 109, 110, 111, 112, 113, 114, whereinsaid positions correspond to amino acid positions of SEQ ID NO:2 (e.g.using the numbering of SEQ ID NO:2),

iii) region 12 corresponding to amino acids 139 to 209 of SEQ ID NO:2,e.g. said alteration at one or more positions selected from the groupconsisting of positions: 139, 140, 141, 142, 143, 144, 145, 146, 147,148, 149, 150, 151, 152, 153, 154, 155, 156, 157, 158, 159, 160, 161,162, 163, 164, 165, 166, 167, 168, 169, 170, 171, 172, 173, 174, 175,176, 177, 178, 179, 180, 181, 182, 183, 184, 185, 186, 187, 188, 189,190, 191, 192, 193, 194, 195, 196, 197, 198, 199, 200, 201, 202, 203,204, 205, 206, 207, 208, 209, wherein said positions correspond to aminoacid positions of SEQ ID NO:2 (e.g. using the numbering of SEQ ID NO:2),

iv) region 13 corresponding to amino acids 252 to 266 of SEQ ID NO:2,e.g. said alteration at one or more positions selected from the groupconsisting of positions: 252, 253, 254, 255, 256, 257, 258, 259, 260,261, 262, 263, 264, 265, 266, wherein said positions correspond to aminoacid positions of SEQ ID NO:2 (e.g. using the numbering of SEQ ID NO:2),

v) region 14 corresponding to amino acids 302 to 338 of SEQ ID NO:2,e.g. said alteration at one or more positions selected from the groupconsisting of positions: 302, 303, 304, 305, 306, 307, 308, 309, 310,311, 312, 313, 314, 315, 316, 317, 318, 319, 320, 321, 322, 323, 324,325, 326, 327, 328, 329, 330, 331, 332, 333, 334, 335, 336, 337, 338,wherein said positions correspond to amino acid positions of SEQ ID NO:2(e.g. using the numbering of SEQ ID NO:2),

vi) region 15 corresponding to amino acids 362 to 546 of SEQ ID NO:2,e.g. said alteration at one or more positions selected from the groupconsisting of positions: 362, 363, 364, 365, 366, 367, 368, 369, 370,371, 372, 373, 374, 375, 376, 377, 378, 379, 380, 381, 382, 383, 384,385, 386, 387, 388, 389, 390, 391, 392, 393, 394, 395, 396, 397, 398,399, 400, 401, 402, 403, 404, 405, 406, 407, 408, 409, 410, 411, 412,413, 414, 415, 416, 417, 418, 419, 420, 421, 422, 423, 424, 425, 426,427, 428, 429, 430, 431, 432, 433, 434, 435, 436, 437, 438, 439, 440,441, 442, 443, 444, 445, 446, 447, 448, 449, 450, 451, 452, 453, 454,455, 456, 457, 458, 459, 460, 461, 462, 463, 464, 465, 466, 467, 468,469, 470, 471, 472, 473, 474, 475, 476, 477, 478, 479, 480, 481, 482,483, 484, 485, 486, 487, 488, 489, 490, 491, 492, 493, 494, 495, 496,497, 498, 499, 500, 501, 502, 503, 504, 505, 506, 507, 508, 509, 510,511, 512, 513, 514, 515, 516, 517, 518, 519, 520, 521, 522, 523, 524,525, 526, 527, 528, 529, 530, 531, 532, 533, 534, 535, 536, 537, 538,539, 540, 541, 542, 543, 544, 545, 546, wherein said positionscorrespond to amino acid positions of SEQ ID NO:2 (e.g. using thenumbering of SEQ ID NO:2),

vii) region 16 corresponding to amino acids 596 to 611 of SEQ ID NO:2,e.g. said alteration at one or more positions selected from the groupconsisting of positions: 596, 597, 598, 599, 600, 601, 602, 603, 604,605, 606, 607, 608, 609, 610, 611, wherein said positions correspond toamino acid positions of SEQ ID NO:2 (e.g. using the numbering of SEQ IDNO:2),

viii) region 17 corresponding to amino acids 661 to 805 of SEQ ID NO:2,e.g. said alteration at one or more positions selected from the groupconsisting of positions: 661, 662, 663, 664, 665, 666, 667, 668, 669,670, 671, 672, 673, 674, 675, 676, 677, 678, 679, 680, 681, 682, 683,684, 685, 686, 687, 688, 689, 690, 691, 692, 693, 694, 695, 696, 697,698, 699, 700, 701, 702, 703, 704, 705, 706, 707, 708, 709, 710, 711,712, 713, 714, 715, 716, 717, 718, 719, 720, 721, 722, 723, 724, 725,726, 727, 728, 729, 730, 731, 732, 733, 734, 735, 736, 737, 738, 739,740, 741, 742, 743, 744, 745, 746, 747, 748, 749, 750, 751, 752, 753,754, 755, 756, 757, 758, 759, 760, 761, 762, 763, 764, 765, 766, 767,768, 769, 770, 771, 772, 773, 774, 775, 776, 777, 778, 779, 780, , 781,782, 783, 784, 785, 786, 787, 788, 789, 790, 791, 792, 793, 794, 795,796, 797, 798, 799, 800, 801, 802, 803, 804, 805, wherein said positionscorrespond to amino acid positions of SEQ ID NO:2 (e.g. using thenumbering of SEQ ID NO:2),

ix) region 18 corresponding to amino acids 829 to 838 to 1042 of SEQ IDNO:2, e.g. said alteration at one or more positions selected from thegroup consisting of positions: 829, 830, 831, 832, 833, 834, 835, 836,837, 838, wherein said positions correspond to amino acid positions ofSEQ ID NO:2 (e.g. using the numbering of SEQ ID NO:2)

x) region 19 corresponding to amino acids 1043 to 1055 of SEQ ID NO:2,e.g. said alteration at one or more positions selected from the groupconsisting of positions 1043, 1044, 1045, 1046, 1047, 1048, 1049, 1050,1051, 1052, 1053, 1054, 1055, wherein said positions correspond to aminoacid positions of SEQ ID NO:2 (e.g. using the numbering of SEQ ID NO:2);and/or

b) one or more adjacent regions, said adjacent region being selectedfrom the group consisting of: (i′) region 1 corresponding to amino acids95 to 105 of SEQ ID NO:2; (ii′) region 2 corresponding to amino acids115 to 138 of SEQ ID NO:2; (iii′) region 3 corresponding to amino acids210 to 251 of SEQ ID NO:2; (iv′) region 4 corresponding to amino acids267 to 301 of SEQ ID NO:2; (v′) region 5 corresponding to amino acids339 to 361 of SEQ ID NO:2; (vi′) region 6 corresponding to amino acids547 to 595 of SEQ ID NO:2; (vii') region 7 corresponding to amino acids612 to 660 of SEQ ID NO:2; (viii′) region 8 corresponding to amino acids806 to 828 of SEQ ID NO:2; and (ix′) region 9 corresponding to aminoacids 839 to 1042 of SEQ ID NO:2.

In a particular aspect, the endoglucanase variant as described herein isone that does not comprise any amino acid alteration at a positionoutside of regions 10, 11, 12, 13, 14, 15, 16, 17, 18, and 19. In thisaspect, the endoglucanase variant thus does not comprise any alteration(e.g. a substitution, deletion or insertion, preferably a substitution)in a region selected from the group consisting of: region 1corresponding to amino acids 95 to 105 of SEQ ID NO:2, region 2corresponding to amino acids 115 to 138 of SEQ ID NO:2, region 3corresponding to amino acids 210 to 251 of SEQ ID NO:2, region 4corresponding to amino acids 267 to 301 of SEQ ID NO:2, region 5corresponding to amino acids 339 to 361 of SEQ ID NO:2, region 6corresponding to amino acids 547 to 595 of SEQ ID NO:2, region 7corresponding to amino aicds 612 to 660 of SEQ ID NO:2, region 8corresponding to amino acids 806 to 828 of SEQ ID NO:2, and region 9corresponding to amino acids 839 to 1045 of SEQ ID NO:2. Alternatively,the endoglucanase variant as described herein is one that does notcomprise any amino acid alteration at a position outside of regions 1,2, 3, 4, 5, 6, 7, 8, and 9. In this aspect, the endoglucanase variantthus does not comprise any alteration (e.g. a substitution, deletion orinsertion, preferably a substitution) in a region selected from thegroup consisting of: region 10, region 11, region 12, region 13, region14, region 15, region 16, region 17, region 18, and region 19. It ishowever preferred that the endoglucanase variant comprises at least onealteration in any of regions 1-9 and at least one alteration in any oneregions 10-19.

In some aspects, the detergent composition comprises an endoglucanasevariant having an alteration (e.g. a substitution, deletion orinsertion, preferably a substitution) at one or more positions in: (i)regions 6 and 17; (ii) regions 6, 15 and 17; (iii) regions 10, 12 and15; (iv) regions 6, 7, 16, and 17; (v) region 6, 9, 10, 12, 15, and 17;(vi) region 14 and 15; (vii) region 9; (viii) 6, 7, 9, 14, 15, 16, and17; or (ix) 3, 6, 7, 9, 14, 15, 16, and 17; wherein said variantpreferably has no alternation in the other regions besides thosementioned.

In some aspects, the detergent composition comprises an endoglucanasevariant having an alteration (e.g. a substitution, deletion orinsertion, preferably a substitution) at one or more positions selectedfrom the group consisting of positions: 4, 17, 18, 20, 51, 53, 55, 56,60, 63, 71, 79, 87, 92, 99, 120, 125, 126, 130, 137, 182, 186, 189, 192,213, 216, 221, 226, 228, 230, 231, 232, 233, 235, 240, 243, 247, 249,278, 279, 281, 283, 285, 289, 292, 294, 298, 302, 311, 313, 333, 346,353, 358, 386, 387, 388, 390, 403, 408, 410, 416, 441, 448, 451, 471,472, 476, 489, 507, 512, 515, 538, 555, 556, 557, 558, 559, 560, 561,562, 563, 564, 567, 568, 570, 575, 578, 579, 580, 581, 583, 589, 590,591, 592, 593, 595, 598, 599, 602, 603, 605, 607, 609, 616, 627, 630,631, 635, 636, 638, 639, 640, 641, 642, 643, 644, 651, 676, 683, 688,690, 694, 698, 699, 706, 711, 713, 1719, 720, 744, 749, 754, 756, 760, ,781, 786, 797, 810, 811, 812, 815, 823, 824, 825, 827, 828, 833, 834,835, 837, 843, 848, 868, 869, 870, 871, 872, 873, 874, 880, 881, 883,884, 885, 887, 888, 890, 892, 894, 898, 905, 906, 912, 920, 921, 924,926, 927, 928, 932, 933, 934, 935, 937, 938, 939, 940, 941, 942, 943,946, 948, 950, 952, 953, 954, 956, 957, 960, 966, 971, 972, 980, 989,991, 994, 995, 998, 999, 1006, 1009, 1010, 1011, 1029, 1030, 1031, 1032,1035, 1037, 1038, 1040, 1041, 1042, 1044, 1045, and 1048, whereinnumbering is according to SEQ ID NO:2.

In one alternative embodiment, the endoglucanase variant comprises analteration in one or more positions selected from the group of: 285,333, 353, 558, 633, 635, 638, 639, 994, 281, 563, 575, 921,558+559+560+561+562, 558, 559, 560, 561, 562 125, 126, 130, 213, 221,228, 230, 231, 232, 235, 240, 243, 249, 278, 292, 297, 346, 556, 564,565, 567, 568, 569, 570, 576, 578, 579, 580, 583, 589, 590, 591, 592,593, 616, 627, 630, 636, 641, 642, 643, 644, 651, 810, 811, 812, 815,823, 824, 825, 827, 843, 870, 871, 872, 873, 874, 881, 883, 884, 885,887, 894, 920, 932, 933, 934, 935, 937, 938, 939, 940, 941, 942, 943,950, 952, 953, 954, 960, 964, 966, 971, 974, 989, 991, 995, 998, 1006,1010, 1011, 1029, 1030, 1031, 1032, 1035, 1037, 1038, 1040, 1041, 1044,1045, 559+579, 564+579, 562+579, 559+579+99, 99, 559+579+281,281+559+579, 559+579+616, 559+579+636, 559+579+651, 559+579+948, 948,559+579+1009, 1009, 559+579+627, 579+921, 559+579+921, 99+579, 579+651,579+948, 579+1009, 559+579+934, 934, 559+579+921+934, 559+579+627,559+579+627+616, 559+579+627, 559+579+921+651, 559+579+921+627,559+579+921+636, 559+579+921+616, 559+579+921+636, 559+579+921+627+636,559+579+636+651, 559+579+616+651, 559+579+616+636, 559+579+616+921+934,559+579+651+627, 559+579+651+636, 559+579+651+627+636, 559+579+651+616,559+579+651+921+934, 636+934, 636+921, 636+627, 636+579, 638+934,638+921, 638+627, 638+579,627+51, 51,627+451, 451, 627+559,627+579,579+934, 651+638, 570+651, 570+921, 570+627, 570+559, 570+579, 570+638,570+579,570+638,570+651,570+636,570+934,570+638,570+921,570+627,570+559,570+885,885+934,885+627, 559+579+636, 559+579+638, 559+579+870, 559+579+560,559+579+564, 559+579+570, 559+579+570, 559+579+570, 559+579+570,559+579+570, 559+579+570, 559+579+570, 559+579+570, 559+570+579,559+570+579, 559+570+579, 559+570+579, 559+570+579, 559+570+579,559+570+579, 559+570+579, 559+560+579, 559+579+651, 559+579+651+934,559+579+638, 559+579+921, 559+579+616+921, 559+579+636, 559+579,559+579, 559+579+921, 559+579+616, 638+934,627+636,627+934,570+579,416+559+579+636, 128+559+579+627, 128+559+579+636, and 579+636 of SEQ IDNO:2.

In some aspects, the detergent composition comprises an endoglucanasevariant having an alteration (e.g. a substitution, deletion orinsertion, preferably a substitution) at one or more positions selectedfrom the group consisting of: N285G, W333L, T353D, N558NP, N558F, T633V,D635L, D635M, D635T, F638Y, T639D, G994N, and K281T, G563E, 1575M,1575A, K921D, N558K+A559K+S560F+T561P+G562W, N558K, A559K, S560F, T561P,G562W and 1125V, A126R, K130R, K213R, A221R, K228E, K2281, G230F, G230L,G230A, G230H, G230N, G230W, G230T, F231Y, F231N, V232R, V232G, H235D,N240Q, G243K, G243R, A249N, A278S, K281F, K281V, K281Y, K281H, K281Q,K281N, K281W, N285L, N285M, N285S, N285P, N285T, N285Y, N285H, N285K,N285D, N285W, N285R, T292F, T292L, T2921, T292V, T292S, T292P, T292Y,T292Q, T292N, T292K, T292D, T292G, F297L, A346H, G556S, N558D, N558M,N558Q, N5581, N558Y, N558H, A559N, A559F, A559M, A559P, A559Y, A559H,A559Q, A559D, A559R, A559G, A5591, A559S, S560P, S560K, S560G, S560D,T561P, T561E, T561Q, T561S, T561D, A5641, A564Y, A564H, A564Q, A564K,A564E, E565M, V567F, K568R, L569F, L569Y, L569D, L569E, P570F, P570L,P5701, P570M, P570V, P570S, P570T, P570A, P570Y, P570H, P570Q, P570N,P570K, P570E, P570W, P570R, P570G, 1575D, 1575E, 1576F, 1576M, 1576P,D578R, Y579F, Y579W, V580L, D583M, Q589G, P590S, P590T, P590E, E591L,G592D, S593P, S593H, S593Q, S593N, S593K, S593D, S593E, S593R, S616D,K627L, K627M, K627V, K627S, K627T, K627Q, K627R, 1630F, 1630V, 1630Y,D635A, D635P, D635N, D635K, D635E, D635G, D635W, S636L, S636M, S636A,S636H, S636Q, S636N, S636K, S636R, F6381, F638V, F638T, F638L, F638H,T639V, T639S, T639L, T6391, T639M, T639A, T639E, T639W, T639G, Y641E,S642T, S642N, N643D, N643H, N643T, T644F, A651P, S810R, A811S, V812F,V8121, V812M, V812W, V812R, N815V, N815Y, N815E, N815W, N815R, S823Q,A824T, T825N, T825W, T825A, T825D, V8271, V827M, V827S, T843V, D870F,D870L, D8701, D870M, D870V, D870S, D870T, D870Y, D870H, D870Q, D870N,D870K, D870E, D870W, D870R, D870G, P871F, P871L, P8711, P871M, P871V,P871S, P871T, P871A, P871Y, P871H, P871Q, T872S, T872F, T872A, T872Y,T872H, T872Q, T872N, T872K, T872D, T872E, T872W, T872R, T872G, D873K,D873E, T874V, T874S, T874P, T874A, T874H, T874Q, T874N, T874K, V881Q,T883K, Y884H, A885F, A885Q, A885N, T887L, T8871, T887S, T887H, T887R,K894E, N920D, K921R, K921E, T932A, N933V, N933S, Y934G, Y934M, Y934S,Y934A, Y934Q, Y934N, Y934E, Y934W, Y934R, T935W, A937F, A937V, A937S,A937T, A937Q, A937D, A937E, V9381, K9391, K939V, D940E, N941S, N941H,N941D, A942P, A942E, D943Y, D943H, R950V, R950H, R950N, F952S, F952W,N953Y, G954L, Y960F, A964N, A964C, N966P, N966C, G971A, Q974K, Q974C,Q9891, Q991L, Q9911, Q991M, Q991V, Q991T, Q991K, Q991C, S9951, S995V,S995Q, S995R, S995C, G998V, G998A, 51006T, 51006A, S1006K, 51006R,Y1010W, L1011M, L1011S, L1011A, L1011Q, L1011N, L1011D, L1011E, R1029N,F1030M, K10311, K1031S, K1031T, K1031H, V1032G, K1035A, A1037E, A1037W,51038L, S10381, L1040N, L1040E, G1041F, L1044F, L1044S, L1044N, L1044W,P1045Q, P1045W, and A559N+Y579F, A559N, Y579F, A564E+Y579F, A564E,Y579F, A559N+Y579W, A559N, Y579W, G562P+Y579W, G562P, Y579W,A564D+Y579W, A564D, Y579W, A559N+Y579W+K99R, A559N, Y579W, K99R,A559N+Y579W+K281R, A559N, Y579W, K281R, K281R+A559N+Y579W, K281R, A559N,Y579W, A559N+Y579W+S616D, A559N, Y579W, S616D, A559N+Y579W+S636N, A559N,Y579W, S636N, A559N+Y579W+A651P, A559N, Y579W, A651P, A559N+Y579W+K948E,A559N, Y579W, K948E, A559N+Y579W+K1009E, A559N, Y579W, K1009E,A559N+Y579W+K627R, A559N, Y579W, K627R, Y579W+K921R, Y579W, K921R,A559N+Y579W+K921R, A559N, Y579W, K921R, K99R+Y579W, K99R, Y579W,Y579W+A651P, Y579W, A651P, Y579W+K948E, Y579W, K948E, Y579W+K1009E,Y579W, K1009E, A559N+Y579W+Y934G, A559N, Y579W, Y934G,A559N+Y579W+K921R+Y934G, A559N, Y579W, K921R, Y934G, A559N+Y579W+K627M,A559N, Y579W, K627M, A559N+Y579W+K627R+S616D, A559N, Y579W, K627R,S616D, A559N+Y579F+K627R, A559N, Y579F, K627R, A559N+Y579W+K921R+A651P,A559N, Y579W, K921R, A651P, A559N+Y579W+K921R+K627R, A559N, Y579W,K921R, K627R, A559N+Y579W+K921R+S636K, A559N, Y579W, K921R, S636K,A559N+Y579W+K921R+S616D, A559N, Y579W, K921R, S616D,A559N+Y579W+K921R+S636N, A559N, Y579W, K921R, S636N,A559N+Y579W+K921R+K627R+S636N, A559N, Y579W, K921R, K627R, S636N,A559N+Y579W+S636N+A651P, A559N, Y579W, S636N, A651P,A559N+Y579W+S616D+A651P, A559N, Y579W, S616D, A651P,A559N+Y579W+S616D+S636K, A559N, Y579W, S616D, S636K,A559N+Y579W+S616D+K921R+Y934G, A559N, Y579W, S616D, K921R, Y934G,A559N+Y579W+A651P+K627M, A559N, Y579W, A651P, K627M,A559N+Y579W+A651P+S636K, A559N, Y579W, A651P, S636K,A559N+Y579W+A651P+K627R+S636N, A559N, Y579W, A651P, K627R, S636N,A559N+Y579W+A651P+S616D, A559N, Y579W, A651P, S616D,A559N+Y579W+A651P+K921R+Y934G, A559N, Y579W, A651P, K921R, Y934G,S636N+Y934G, S636N, Y934G, S636N+K921R, S636N, K921R, S636N+K627R,S636N, K627R, S636N+Y579W, S636N, Y579W, F6381+Y934G, F6381, Y934G,F638I+K921R, F6381, K921R, F6381+K627R, F6381, K627R, F6381+Y579W,F6381, Y579W, K627R+K51Q, K627R, K51Q, K627R+K451S, K627R, K451S,K627R+A559N, K627R, A559N, K627R+Y579W, K627R, Y579W, Y579W+Y934G,Y579W, Y934G, A651P+F638I, A651P, F6381, P570Q+A651P, P570Q, A651P,P570Q+K921R, P570Q, K921R, P570Q+K627R, P570Q, K627R, P570Q+A559N,P570Q, A559N, P570Q+Y579W, P570Q, Y579W, P570Q+F6381, P570Q, F6381,P570K+Y579W, P570K, Y579W, P570K+F6381, P570K, F6381, P570T+A651P,P570T, A651P, P570T+S636N, P570T, S636N, P570T+Y934G, P570T, Y934G,P570T+F6381, P570T, F6381, P570T+K921R, P570T, K921R, P570T+K627R,P570T, K627R, P570T+A559N, P570T, A559N, P570T+A885F, P570T, A885F,A885F+Y934G, A885F, Y934G, A885F+K627R, A885F, K627R, A559N+Y579W+S636L,A559N, Y579W, S636L, A559N+Y579W+F6381, A559N, Y579W, F6381,A559N+Y579W+D870M, D870M, A559N+Y579W+S560P, S560P, A559N+Y579W+A5641,A5641, A559N+Y579W+P570N, P570N, A559N+Y579W+P570K, P570K,A559N+Y579W+P570R, P570R, A559N+Y579W+P570A, P570A, A559N+Y579W+P570T,P570T, A559N+Y579W+P570S, P570S, A559N+Y579W+P570Q, P570Q,A559N+Y579W+P570H, P570H, and N558E, A559P, A559N, A559H, T561P, A564E,P570A, P570Q, P570R, P570S, P570K, P570T, P570N, Y579W, Y579F, T581M,S616D, K627R, K627M, K627Q, S636N, S636Q, S636R, S636K, S636M, S636H,F6381, F638L, N643D, A651P, A651S, A885F, A885Q, K921R, Y934R, Y934G,N966C, L1011A, K10311, and A559N+P570A+Y579W, A559N+P570H+Y579W,A559N+P570K+Y579W, A559N+P570N+Y579W, A559N+P570Q+Y579W,A559N+P570R+Y579W, A559N+P570T+Y579W, A559N+P570T+Y579W,A559N+S560P+Y579W, A559N+Y579W+A651 P, A559N+Y579W+A651P+Y934G,A559N+Y579W+F6381, A559N+Y579W+K921R, A559N+Y579W+S616D+K921R,A559N+Y579W+S636N, A559N+Y579F, A559N+Y579W, A559N+Y579W+K921R,A559N+Y579W+S616D, F6381+Y934G, K627R+S636N, K627R+Y934G, P570K+Y579W,Q416D+A559N+Y579W+S636N, Q416D, S128X+A559N+Y579W+K627R, S128X,S128X+A559N+Y579W+S636N, Y579W+S636N, V4T, S17A, N18G, F20P, F2ON, F20G,F20Y, K51Q, K51H, E53Y, E53P, E53G, Y55M, Y55D, V56M, Y60F, S63F, A71E,579W, T87R, T92S, A120P, N129D, F137L, H182Y, A186P, N189K, K192N,N216D, N216Q, N216R, L226K, G230H, L233H, D247N, G279E, K281R, A283D,N285D, N285G, Q289E, T292A, T292F, T292Y, A294V, Q298E, 1302D, 1302H,1302V, 1302M, H311N, S313D, A346D, A386P, 1387T, K388R, K390Q, 1403Y,E408D, E408N, E4085, E408P, E408A, E408G, P410G, Q4165, Q416D, N441G,A448E, A448W, A4485, K451S, K451Q, G471S, S472Y, D476R, Q489P, K507R,K512P, S515V, S538C, L555Q, G557R, N558E, A559N, A559P, A559H, A559D,S560P, 5560G, T561P, A564E, A5641, V567P, K568R, P570R, P570Q, P570K,P570A, P570T, P570G, P570S, P570H, P570N, 1575V, Y579W, Y579F, T581M,S593N, S593E, S595L, S598Q, A599S, 1602T, 1602D, V603P, 5605T, S607C,G609E, S616G, S616D, K627R, K627M, K627Q, K631R, K631A, D635A, D635E,D635M, D635N, D635L, D635W, S636N, S636K, S636L, S636Q, S636R, S636M,5636H, F638N, F6381, F638L, F638V, F638H, F638M, T639G, T6391, T639M,T639Y, T639W, T639P, T639E, T640S, S642N, S642T, N643D, N643H, A651P,A651S, D676H, Q683E, A688G, Y690F, T694A, T697G, R698W, T699A, T706Q,T7115, T711V, T711Y, K713R, W719R, K720H, K744H, K744Q, A749T, K754R,V756Y, V756H, 5760G, T, 781M, N786K, T797S, S810Q, A824D, T825G, N828D,N833D, Q834E, S835A, S835D, V8371, N848D, A868E, A869V, D870V, T872G,T872H, T872W, T872Q, R880K, V881Q, V881T, T883R, T883V, T883C, T883K,Y884H, A885N, A885Q, A885F, T887K, T887S, L888M, V890R, T892P, T892V,R898Q, N905D, F906A, Q912V, N920P, K921R, A924D, V926F, V926P, K927R,S928D, T932A, N933S, N933V, Y934G, Y934R, Y934Q, A937E, V9381, K939V,N941S, A942P, G946R, K948R, Q956Y, Q956S, A957L, A957P, N966C, T972K,M9801, G994D, T999R, L1011A, K10311, A1037E, S1038G, G1041R, Y1042N, andF1048W.

In some aspects, the present invention relates to a detergentcomposition comprising an endoglucanase variant having an alteration(e.g. a substitution, deletion or insertion, preferably a substitution)at one or more positions selected from the group consisting ofpositions: 17, 20, 51, 53, 55, 56, 60, 63, 79, 87, 192, 302, 387, 388,390, 403, 408, 410, 416, 448, 451, 471, 472, 507, 512, 515, 538, 598,602, 605, 609, 676, 694, 698, 699, 711, 754, 760, , 781, 786, 797, 834,and 835 of SEQ ID NO:2.

In some aspects, the present invention relates to a detergentcomposition comprising an endoglucanase variant having an alteration(e.g. a substitution, deletion or insertion, preferably a substitution)at one or more positions selected from the group consisting of: S17A,F20P, F20N, F20G, F20Y, K51Q, K51H, E53P, E53G, Y55M, V56M, Y60F, S63F,T87R, K192N, I302H, I302V, I302M, I387T, K388R, K390Q, I403Y, E408D,E408S, E408P, E408A, E408G, E408N, P410G, Q416S, Q416D, A448E, A448W,A448S, K451S, G471S, S472Y, K507R, K512P, S515V, S538C, Y579W, S598Q,I1602T, I602D, S605T, G609E, D676H, T694A, R698W, T699A, T711V, T711Y,K754R, S760G, T, 781M, N786K, T797S, Q834E, and S835D of SEQ ID NO:2.

In some aspects, the present invention relates to a detergentcomposition comprising an endoglucanase variant having an alteration(e.g. a substitution, deletion or insertion, preferably a substitution)in the positions selected from the group consisting of positions:559+579+697; 512+559+579+697; 18+71+186+408+579+602+651+688+756;18+189+408+559+579+688+697+756+921+934; 313+488; 880+905+921+934;302+313+408+579+602+651+697+880+921+934; or216+313+408+476+579+602+638+651+697+719+880+887+921+934 of SEQ ID NO:2.

In some aspects, the present invention relates to a detergentcomposition comprising an endoglucanase variant having an alteration(e.g. a substitution, deletion or insertion, preferably a substitution)selected from the group consisting of the following alterations:A559N+Y579W+T697G; K512P+A559N+Y579W+T697G;N18G+A71E+A186P+E408D+Y579W+1602T+A651P+A688G+V756Y;N18G+N189K+E408D+A559N+Y579W+A688G+T697G+V756Y+K921R+Y934G; S313D+E408D;R880K+N905D+K921R+Y934G;I302D+S313D+E408D+Y579W+I602T+A651P+T697G+R880K+K921R+Y934G; andN216Q+S313D+E408D+D476R+Y579W+I602T+F638N+A651P+T697G+W719R+R880K+T887K+K921R+Y934G of SEQ ID NO:2.

In some aspects, the present invention relates to a detergentcomposition comprising an endoglucanase variant having activity onxanthan gum pre-treated with xanthan lyase; preferably said activitycomprises endoglucanase EC 3.2.1.4 activity, further preferably saidactivity is endoglucanase EC 3.2.1.4 activity.

In some aspects, the present invention relates to a detergentcomposition comprising an endoglucanase variant having an improvedstability in a detergent composition compared to a parent endoglucanase(e.g. with SEQ ID NO:2).

In some aspects, the present invention relates to a detergentcomposition comprising an endoglucanase variant having a half-life whichis improved over the parent endoglucanase or a wild-type endoglucanase.

In one embodiment, the endoglucanase variant has a half-life of at least1.5 h when measured at a temperature of 25° C. and in a detergentconcentration of 90%. In a particular embodiment, the half-life ismeasured as described in Examples 3 and 7.

In some aspects, the present invention relates to a detergentcomposition comprising an endoglucanase variant having a half-lifeimprovement factor (HIF) of >1.0 relative to a parent endoglucanase,e.g. an endoglucanase of SEQ ID NO:2.

In some aspects, the invention relates to a detergent compositioncomprising an isolated GH9 endoglucanase variant having activity onxanthan gum pretreated with xanthan lyase.

In some aspects, the detergent composition comprises a xanthan lyasevariant comprising an alteration (e.g. a substitution, deletion orinsertion, preferably a substitution) at one or more positions in two ormore, three or more, four or more, five or all six regions selected fromthe group consisting of: (i) region 1 corresponding to amino acids 154to 176 of SEQ ID NO:6; (ii) region 2 corresponding to amino acids 614 to658 of SEQ ID NO:6; (iii) region 3 corresponding to amino acids 731 to803 of SEQ ID NO:6; (iv) region 4 corresponding to amino acids 807 to846 of SEQ ID NO:6; (v) region 5 corresponding to amino acids 872 to 885of SEQ ID NO:6; and (vi) region 6 corresponding to amino acids 903 to1004 of SEQ ID NO:6.

In some aspects, the detergent compositions comprise a xanthan lyasevariant comprising an alteration (e.g. a substitution, deletion orinsertion, preferably a substitution) at one or more positions in two ormore regions selected from the group consisting of: (i) region 7corresponding to amino acids 1 to 153 of SEQ ID NO:6; (ii) region 8corresponding to amino acids 177 to 613 of SEQ ID NO:6; (iii) region 9corresponding to amino acids 659 to 730 of SEQ ID NO:6; (iv) region 10corresponding to amino acids 804 to 806 of SEQ ID NO:6; (v) region 11corresponding to amino acids 847 to 871 of SEQ ID NO:6; (vi) region 12corresponding to amino acids 886 to 902 of SEQ ID NO:6; and (vii) region13 corresponding to amino acids 1005 to 1037 of SEQ ID NO:6.

In some aspects, the detergent composition comprises a xanthan lyasevariant comprising an alteration (e.g. a substitution, deletion orinsertion, preferably a substitution) at one or more positions in two ormore, three or more, four or more, five or more, six or more, seven ormore, eight or more, nine or more, ten or more, eleven or more, twelveor all thirteen regions selected from the group consisting of: (i)region 1 corresponding to amino acids 154 to 176 of SEQ ID NO:6; (ii)region 2 corresponding to amino acids 614 to 658 of SEQ ID NO:6; (iii)region 3 corresponding to amino acids 731 to 803 of SEQ ID NO:6; (iv)region 4 corresponding to amino acids 807 to 846 of SEQ ID NO:6; (v)region 5 corresponding to amino acids 872 to 885 of SEQ ID NO:6; (vi)region 6 corresponding to amino acids 903 to 1004 of SEQ ID NO:6; (vii)region 7 corresponding to amino acids 1 to 153 of SEQ ID NO:6; (iii)region 8 corresponding to amino acids 177 to 613 of SEQ ID NO:6; (xi)region 9 corresponding to amino acids 659 to 730 of SEQ ID NO:6; (x)region 10 corresponding to amino acids 804 to 806 of SEQ ID NO:6; (xi)region 11 corresponding to amino acids 847 to 871 of SEQ ID NO:6; (xii)region 12 corresponding to amino acids 886 to 902 of SEQ ID NO:6; and(xiii) region 13 corresponding to amino acids 1005 to 1037 of SEQ IDNO:6.

In some aspects of the detergent compoitions of the invention, thexanthan lyase variant comprises an alteration at one or more positionsin at least one chelator-induced instability region as well as analteration at one or more positions in at least one adjacent region.Thus, in some aspects the xanthan lyase variant, in addition to analteration in one or more positions in at least one region selected fromthe group consisting of regions 1, 2, 3, 4, 5 and 6 as set forth aboveand elsewhere herein, further comprises an alteration (e.g. asubstitution, deletion or insertion, preferably a substitution) at oneor more positions in at least one region selected from the groupconsisting of: (vii) region 7 corresponding to amino acids 1 to 153 ofSEQ ID NO:6; (viii) region 8 corresponding to amino acids 177 to 613 ofSEQ ID NO:6; (ix) region 9 corresponding to amino acids 659 to 730 ofSEQ ID NO:6; (x) region 10 corresponding to amino acids 804 to 806 ofSEQ ID NO:6; (xi) region 11 corresponding to amino acids 847 to 871 ofSEQ ID NO:6; (xii) region 12 corresponding to amino acids 886 to 902 ofSEQ ID NO:6; and (xiii) region 13 corresponding to amino acids 1005 to1037 of SEQ ID NO:6.

The xanthan lyase variant may e.g. comprise an alteration at one or morepositions in each of one or more, two or more, three or more, four ormore, five or more, six or all seven regions selected from the groupconsisting of regions 7, 8, 9, 10, 11, 12 and 13.

In a particular aspect, the xanthan lyase variant as described herein isone that does not comprise any amino acid alteration at a positionoutside of regions 7, 8, 9, 10, 11, 12 and 13. In this aspect, thexanthan lyase variant thus does not comprise any alteration (e.g. asubstitution, deletion or insertion, preferably a substitution) in aregion selected from the group consisting of: region 1 corresponding toamino acids 154 to 176 of SEQ ID NO:6, region 2 corresponding to aminoacids 614 to 658 of SEQ ID NO:6, region 3 corresponding to amino acids731 to 803 of SEQ ID NO:6, region 4 corresponding to amino acids 807 to846 of SEQ ID NO:6, region 5 corresponding to amino acids 872 to 885 ofSEQ ID NO:6, and region 6 corresponding to amino acids 903 to 1004 ofSEQ ID NO:6. Alternatively, the xanthan lyase variant as describedherein is one that does not comprise any amino acid alteration at aposition outside of regions 1, 2, 3, 4, 5 and 6. In this aspect, thexanthan lyase variant thus does not comprise any alteration (e.g. asubstitution, deletion or insertion, preferably a substitution) in aregion selected from the group consisting of: region 7, region 8, region9, region 10, region 11, region 12, and region 13. It is howeverpreferred that the endoglucanase variant comprises at least onealteration in any of regions 1-6 and at least one alteration in any oneregions 7-13.

In some aspects, the xanthan lyase variant as described herein is onethat comprises an alteration (e.g. a substitution, deletion orinsertion, preferably a substitution) at one or more positions in: (i)regions 3 and 5; (ii) regions 3, 5 and 12; (iii) regions 8, and 9; (iv)regions 2, 3, and 5; (v) regions 2, 3, 5, and 12; (vi) regions 3, 5, 8,9, and 12; (vii) regions 2, 3, 5, 8, and 9; (viii) 3, 5, 8, 9, and 12;(ix) 2, 3, 5, 8, 9, and 12; (x) region 3; (xi) regions 3, 4 and 5; (xii)regions 7, 8 and 9; (xiii) regions 12 and 13; (xiv) regions 3, 4, 5, 8,9, and 12; (xv) regions 8, 9, 12, and 13; (xvi) regions 7, 8, 9, 12, and13; (xvii) regions 3, 4, 5, 7, 8, 9, and 12; and (xviii) regions 3, 4,5, 7, 8, 9, 12, and 13, wherein said variant preferably has noalteration in the other regions besides those mentioned.

In some aspects, the detergent composition comprises a xanthan lyasevariant having an alteration (e.g. a substitution, deletion orinsertion, preferably a substitution) at one or more positions selectedfrom the group consisting of positions: 155, 159, 620, 624, 626, 631,635, 645, 649, 650, 656, 738, 745, 746, 748, 752, 753, 754, 757, 764,769, 774, 775, 777, 779, 782, 785, 786, 789, 792, 796, 799, 800, 801,819, 824, 843, 845, 875, 903, 911, 912, 915, 919, 921, 923, 925, 927,928, 930, 932, 933, 941, 966, 967, 991 and 998 of SEQ ID NO:6.

In some aspects, the present invention relates to a detergentcomposition comprising a xanthan lyase variant having one or moresubstitutions selected from the group consisting of: Y155E, A159P,K620R, A624E, A626G, T631N, T631E, S635E, S635T, S635Q, A645S, T649V,T649K, T649R, Q650G, 1656V, G738L, K745R, F746L, L748T, P752R, P752K,G753E, G753Q, G753S, S754E, S754L, S754Q, S754R, S757D, S757P, S757E,P764V, P764K, A769D, A769T, A769R, A769S, A769E, A769Q, A769*, A774V,L775M, L775Y, L775A, L7751, L775S, L775F, L775Q, D777K, D777R, P779V,Y7821, A785T, N786K, G789R, K792W, K792Y, K792V, K792A, N796Q, A799H,V800P, D801G, K819R, K819T, K824R, A843P, D845E, 875T, K875E, T903A,T903Q, A911V, A911M, A911S, A912T, A9121, A912Y, T915Q, T915S, T915V,T915A, T919F, T919G, T919D, T921R, T921S, T923H, T923D, T925Q, T925D,T925R, T927K, D928W, Y930H, Y930L, Y930F, A932P, D933M, G941E, G941D,A966P, A967D, N991D and V998K.

In some aspects, the present invention relates to a detergentcomposition comprising a xanthan lyase variant having an alteration(e.g. a substitution, deletion or insertion, preferably a substitution)at one or more positions selected from the group consisting of: 624,635, 649, 656, 738, 753, 754, 757, 769, 775, 777, 801, 843 and 875.

In some aspects, the present invention relates to a detergentcomposition comprising a xanthan lyase variant having one or moresubstitutions selected from the group consisting of: A624E, S635E,T649K, I656V, G738L, G753E, S754E, S754R, S757D, A769D, L775A, D777R,D801G, A843P and K875T.

In some aspects, the alteration at one or more positions in at least oneregion selected from the group consisting of regions 7, 8, 9, 10, 11, 12and 13 is an alteration at one or more positions selected from the groupconsisting of: 9, 15, 18, 46, 58, 66, 89, 95, 100, 106, 109, 183, 188,190, 203, 204, 221, 229, 234, 238, 240, 242, 243, 257, 258, 284, 291,293, 316, 317, 320, 324, 329, 333, 339, 341, 352, 354, 360, 372, 377,399, 400, 419, 440, 450, 451, 454, 458, 481, 492, 505, 533, 567, 568,576, 578, 579, 582, 664, 672, 703, 722, 726, 727, 728, 851, 855, 856,867, 887, 892, 899, 900, 901, 902, 915, 1008 and 1016 of SEQ ID NO:6.The xanthan lyase variant may e.g. comprise an alteration at two or moreof these positions, e.g. at three, four, five, six, seven, eight, nineor ten of these positions.

In some aspects, the alteration at one or more positions in at least oneregion selected from the group consisting of regions 7, 8, 9, 10, 11, 12and 13 comprises one or more substitutions selected from the groupconsisting of: K9R, N15T, T18D, L46D, A58L, S66H, Q89Y, K95E, S100D,N106Y, Q109R, Q109D, Q109F, Q109K, Q109A, K183Q, K183R, V188I, A190Q,A203P, K204R, A221P, E229N, E229S, E229V, I234V, I238W, I238L, I238M,I240W, N242S, G243V, Y257W, R258E, R284G, K291R, A293G, A293P, K316R,R317K, K320R, L324Q, K329R, K333R, L339M, I341P, V3521, S354P, K360G,K360R, Q372H, F377Y, N399K, K400R, F419Y, N440K, D450P, K451E, K451R,A454V, D458S, K481R, A492H, A492L, T505I, L5331, K567R, G568A, S578K,S578N, S578R, S579R, S579K, S582K, T664K, N672D, I703L, I722F, P726Q,T727P, M728V, S851F, K855R, E856D, P867S, K887R, N892Y, N892W, N892F,G899S, I900G, D901A, T902F, N1008D and K1016T of SEQ ID NO:6. Thexanthan lyase variant may e.g. comprise two or more of thesesubstitutions, e.g. three, four, five, six, seven, eight, nine or ten ofsaid substititions.

In some aspects of the detergent compositions of the invention, thexanthan lyase variant comprises an alteration at one or more positionsin at least one region selected from the group consisting of regions 1,2, 3, 4, 5 and 6, and an alteration at one or more positions in at leastone region selected from the group consisting of regions 7, 8, 9, 10,11, 12 and 13. In one aspect, the variant comprises an alteration at oneor more positions selected from the group consisting of positions 624,631, 635, 649, 656, 738, 752, 753, 754, 757, 769, 775, 777, 800, 801,843, 875, 911 and 915, and an alteration at one or more positionsselected from the group consisting of positions 89, 100, 190, 229, 234,352, 360, 399, 440, 458, 492, 567, 582, 664, 672, 703, 728, 892, 1008and 1016 of SEQ ID NO:6.

The variant may, for example, comprise an alteration at two or morepositions, e.g. three, four, five or more positions, selected from thegroup consisting of positions 624, 631, 635, 649, 656, 738, 752, 753,754, 757, 769, 775, 777, 800, 801, 843, 875, 911 and 915, and analteration at two or more positions, e.g. two, three, four, five or morepositions, selected from the group consisting of positions 89, 100, 190,229, 234, 352, 360, 399, 440, 458, 492, 567, 582, 664, 672, 703, 728,892, 1008 and 1016 of SEQ ID NO:6.

Preferred positions for alteration in this aspect include one or morepositions selected from the group consisting of positions 624, 635, 649,656, 738, 753, 754, 757, 769, 775, 777, 801, 843 and 875, and one ormore positions selected from the group consisting of positions 100, 190,229, 234, 360, 399, 440, 458, 492, 567, 582, 672, 892 and 1008 of SEQ IDNO:6.

In one embodment of this aspect, the xanthan lyase variant comprises oneor more substitutions selected from the group consisting of Q89Y, S100D,A190Q, E229S, I234V, V352I, K360G, N399K, N440K, D458S, A492H, A492L,K567R, S582K, T664K, N672D, I703L, M728V, N892Y N1008D and K1016T, andone or more substitutions selected from the group consisting of A624E,T631N, S635E, T649K, 1656V, G738L, P752K, P752R, G753E, S754E, S754R,S757D, A769D, L775A, D777R, V800P, D801G, A843P, K875T, A911V and T915A.The variant may, for example, comprise two or more substitutions, e.g.three, four, five or more substitutions, selected from the groupconsisting of Q89Y, S100D, A190Q, E229S, 1234V, V3521, K360G, N399K,N440K, D458S, A492H, A492L, K567R, S582K, T664K, N672D, 1703L, M728V,N892Y N1008D and K1016T, and two or more substitutions, e.g. three,four, five or more substiutitions, selected from the group consisting ofA624E, T631N, S635E, T649K, 1656V, G738L, P752K, P752R, G753E, S754E,S754R, S757D, A769D, L775A, D777R, V800P, D801G, A843P, K875T, A911V andT915A. Preferred substitutions in this embodiment include one or moresubstitutions selected from the group consisting of S100D, A190Q, E229S,1234V, K360G, N399K, N440K, D458S, A492H, K567R, S582K, N672D, N892Y andN1008D, and one or more substitutions selected from the group consistingof A624E, S635E, T649K, 1656V, G738L, G753E, S754E, S754R, S757D, A769D,L775A, D777R, D801G, A843P and K875T.

Non-limiting examples of such variants include:

-   -   A190Q, E229S, S635E, T649K, 1656V, N672D, I703L, G753E, S754E,        S757D, A769D, L775A, D801G, K875T, N892Y, N1008D    -   E229S, S635E, T649K, I656V, N672D, I703L, G753E, S754E, A769D,        L775A, D801G, K875T, N892Y, N1008D    -   E229S, V3521, S635E, T649K, 1656V, N672D, G753E, S754E, A769D,        L775A, V800P,    -   E229S, K360G, D458S, S582K, N672D, G753E, S754E, A769D, L775A,        D801G, K875T, N892Y, N1008D    -   S100D, E229S, K360G, D458S, S582K, T664K, N672D, G753E, S754E,        S757D, A769D, L775A, D801G, A843P, K875T, N892Y, A911V, N1008D,        K1016T    -   E229S, I234V, S582K, N672D, G753E, S754E, A769D, L775A, V800P,        D801G, K875T, N892Y    -   Q89Y, E229S, N440K, S582K, A624E, N672D, G753E, S754E, A769D,        L775A, D801G, K875T, N892Y    -   E229S, S635E, T649K, I656V, N672D, P752K, G753E, A769D, L775A,        D801G, A843P, K875T, N892Y    -   E229S, S635E, T649K, I656V, N672D, G753E, S754E, S757D, A769D,        L775A, D801G, K875T, N892Y    -   E229S, N440K, S582K, N672D, G753E, S754E, A769D, L775A, D801G,        A843P, K875T, N892Y, N1008D    -   E229S, N440K, S582K, A624E, N672D, G753E, S754E, A769D, L775A,        V800P, D801G, K875T, N892Y    -   A190Q, E229S, S635E, T649K, I656V, N672D, P752K, G753E, A769D,        L775A, D801G, A843P, K875T, N892Y    -   A190Q, E229S, S582K, N672D, G753E, S754E, A769D, L775A, D801G,        K875T, N892Y    -   E229S, N440K, S582K, N672D, P752R, G753E, S754E, S757D, A769D,        L775A, D801G, K875T, N892Y, N1008D    -   E229S, S582K, S635E, N672D, P752R, G753E, S754E, A769D, L775A,        D801G, K875T, N892Y, N1008D    -   A190Q, E229S, N440K, S582K, A624E, S635E, N672D, G753E, S754E,        A769D, L775A, D801G, K875T, N892Y    -   E229S, I234V, A492L, S582K, N672D, G753E, S754E, A769D, L775A,        D801G, K875T, N892Y    -   A190Q, E229S, K360G, D458S, S582K, T664K, N672D, G753E, S754E,        A769D, L775A, D801G, K875T, N892Y, N1008D    -   S100D, E229S, K360G, D458S, S582K, N672D, G753E, S754E, S757D,        A769D, L775A, D801G, A843P, K875T, N892Y, T915A, N1008D

E229S, N440K, S582K, A624E, S635E, N672D, G738L, G753E, S754E, S757D,A769D, L775A, D801G, K875T, N892Y

-   -   S100D, E229S, K360G, D458S, S582K, N672D, G753E, S754E, A769D,        L775A, D801G, K875T, N892Y, N1008D    -   A190Q, E229S, D458S, T631N, N672D, G753E, S754E, A769D, L775A,        D801G, A843P, K875T, N892Y    -   A190Q, E229S, K360G, D458S, S582K, N672D, G753E, S754E, A769D,        L775A, D801G, K875T, N892Y, N1008D    -   E229S, S635E, T649K, 1656V, N672D, G753E, S754R, S757D, A769D,        L775A, D801G, A843P, K875T, N892Y    -   E229S, D458S, S582K, T631N, S635E, N672D, M728V, G753E, S754E,        S757D, A769D, L775A, D801G, K875T, N892Y    -   A190Q, E229S, K360G, D458S, S582K, N672D, G753E, S754E, S757D,        A769D, L775A, D801G, K875T, N892Y, N1008D    -   E229S, A492L, S635E, T649K, 1656V, N672D, G753E, S757D, A769D,        L775A, D801G, K875T, N892Y    -   S100D, A190Q, E229S, K360G, D458S, S582K, N672D, G753E, S754E,        A769D, L775A, D801G, K875T, N892Y, N1008D    -   A190Q, E229S, I234V, S582K, N672D, G753E, S754E, S757D, A769D,        L775A, D801G, K875T, N892Y    -   E229S, N399K, D458S, A492H, K567R, S582K, S635E, T649K, N672D,        G753E, S754E, A769D, L775A, D777R, D801G, K875T, N892Y    -   E229S, D458S, A492L, T631N, N672D, G753E, S754E, S757D, A769D,        L775A, D801G, K875T, N892Y    -   E229S, D458S, A492H, K567R, S582K, S635E, N672D, G753E, S754E,        A769D, L775A, D777R, D801G, K875T, N892Y    -   S100D, E229S, K360G, D458S, S582K, N672D, G753E, S754E, S757D,        A769D, L775A, D801G, A843P, K875T, N892Y, N1008D    -   E229S, N399K, D458S, K567R, S582K, S635E, N672D, G753E, S754E,        A769D, L775A, D777R, D801G, K875T, N892Y.

In some aspects, the present invention relates to detergent compositionscomprising a xanthan lyase variant having an alteration (e.g. asubstitution, deletion or insertion, preferably a substitution) at oneor more positions selected from the group consisting of positions: 9,15, 46, 58, 66, 89, 95, 100, 106, 109, 183, 188, 190, 203, 204, 221,229, 234, 238, 240, 242, 243, 257, 258, 291, 293, 316, 320, 324, 329,333, 339, 341, 352, 354, 360, 377, 400, 419, 450, 451, 454, 481, 492,567, 568, 578, 579, 664, 672, 855, 887 and 892 of SEQ ID NO:6.

In some aspects, the present invention relates to detergent compositionscomprising a xanthan lyase variant having one or more substitutionsselected from the group consisting of: K9R, N15T, L46D, A58L, S66H,Q89Y, K95E, S100D, N106Y, Q109R, Q109D, Q109F, Q109K, Q109A, K183Q,K183R, V1881, A190Q, A203P, K204R, A221P, E229N, E229S, I234V, I238W,I238L, I238M, I240W, N242S, G243V, Y257W, R258E, K291R, A293G, A293P,K316R, K320R, L324Q, K329R, K333R, L339M, I341P, V3521, S354P, K360R,F377Y, K400R, F419Y, D450P, K451E, K451R, A454V, K481R, A492L, K567R,G568A, S578K, S578R, S579R, S579K, T664K, N672D, K855R, K887R, N892Y,N892W and N892F.

In some aspects, the present invention relates to a detergentcomposition comprising a xanthan lyase variant having an alteration(e.g. a substitution, deletion or insertion, preferably a substitution)in the positions selected from the group consisting of positions: 190,229, 234, 440, 582, 624, 631, 635, 672, 703, 738, 752, 753, 754, 757,769, 775, 801, 875, 892, and any combination thereof, preferably229+672+752+753+769+775+801+875+892;229+672+753+754+769+775+801+875+892;229+672+752+753+754+769+775+801+875+892;190+229+234+624+672+753+754+769+775+801+875;190+229+631+672+703+752+753+769+775+801+875;190+229+234+582+672+753+754+757+769+775+801+875+892;229+440+582+624+635+672+738+753+754+757+769+775+801+875+892; or100+229+360+458+582+672+753+754+757+769+775+801+843+875+892+1008 of SEQID NO:6.

In some aspects, the present invention relates to a detergentcomposition comprising a xanthan lyase variant having an alteration(e.g. a substitution, deletion or insertion, preferably a substitution)selected from the group consisting of the following alterations:E229N+N672D+P752K+G753E+A769D+L775A+D801G+K875T+N892Y;E2295+N672D+G753E+5754E+A769D+L775A+D801G+K875T+N892Y;E2295+N672D+P752R+G753E+5754E+A769D+L775A+D801G+K875T+N892Y;A190Q+E229S+1234V+A624E+N672D+G753E+S754E+A769D+L775A+D801G+K875T;A190Q+E229S+T631N+N672D+1703L+P752K+G753E+A769D+L775A+D801G+K875T;A190Q+E229S+1234V+S582K+N672D+G753E+S754E+S757D+A769D+L775A+D801G+K875T+N892Y;E229S+N440K+S582K+A624E+S635E+N672D+G738L+G753E+S754E+S757D+A769D+L775A+D801G+K875T+N892Y; orS100D+E229S+K360G+D458S+S582K+N672D+G753E+S754E+S757D+A769D+L775A+D801G+A843P+K875T+N892Y+N1008D of SEQ ID NO:6.

In some aspects, the present invention relates to a detergentcomposition comprising a xanthan lyase variant having activity onxanthan gum; preferably said activity comprises xanthan lyase EC4.2.2.12 activity, further preferably said activity is xanthan lyase EC4.2.2.12 activity.

In some aspects, the present invention relates to a detergentcomposition comprising a xanthan lyase variant having an improvedstability in said detergent composition compared to a parent xanthanlyase (e.g. with SEQ ID NO:6).

In some aspects, the present invention relates to a detergentcomposition comprising a xanthan lyase variant having a half-lifeimprovement factor (HIF) of >1.0 relative to a parent xanthan lyase.

In some aspects, the invention relates to a detergent compositioncomprising an isolated xanthan lyase variant having activity on xanthangum according to the invention.

In some aspects, the present invention relates to a detergentcomposition, as defined herein, comprising an endoglucanase varianthaving at least 61%, 62%, 63%, 64%, 65%, 66%, 67%, 68%, 69%, 70%, 71%,72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%,86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, or 98%sequence identity to SEQ ID NO:2 and having an alteration (e.g. asubstitution, deletion or insertion, preferably a substitution) in thepositions selected from the group consisting of positions: 559+579+697;512+559+579+697; 18+71+186+408+579+602+651+688+756;18+189+408+559+579+688+697+756+921+934; 313+488; 880+905+921+934;302+313+408+579+602+651+697+880+921+934; or216+313+408+476+579+602+638+651+697+719+880+887+921+934 of SEQ ID NO:2.Preferably, the endoglucanase variant has an alteration selected fromthe group consisting of the following alterations: A559N+Y579W+T697G;K512P+A559N+Y579W+T697G;N18G+A71E+A186P+E408D+Y579W+1602T+A651P+A688G+V756Y;N18G+N189K+E408D+A559N+Y579W+A688G+T697G+V756Y+K921R+Y934G; S313D+E408D;R880K+N905D+K921R+Y934G;I302D+S313D+E408D+Y579W+1602T+A651P+T697G+R880K+K921R+Y934G; andN216Q+S313D+E408D+D476R+Y579W+I602T+F638N+A651P+T697G+W719R+R880K+T887K+K921R+Y934G of SEQ ID NO:2. In a particular aspect, the endoglucanasevariant has besides the aforementioned alterations no furtheralterations relative to the parent enzyme of SEQ ID NO:2, i.e. theremaining sequence is identical to SEQ ID NO:2.

In some aspects, the present invention relates to a detergentcomposition, as defined herein, comprising a xanthan lyase varianthaving at least 61%, 62%, 63%, 64%, 65%, 66%, 67%, 68%, 69%, 70%, 71%,72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%,86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, or 98%sequence identity to SEQ ID NO:6 and having an alteration (e.g. asubstitution, deletion or insertion, preferably a substitution) in thepositions selected from the group consisting of positions:229+672+752+753+769+775+801+875+892;229+672+753+754+769+775+801+875+892;229+672+752+753+754+769+775+801+875+892;190+229+234+624+672+753+754+769+775+801+875;190+229+631+672+703+752+753+769+775+801+875;190+229+234+582+672+753+754+757+769+775+801+875+892;229+440+582+624+635+672+738+753+754+757+769+775+801+875+892; or100+229+360+458+582+672+753+754+757+769+775+801+843+875+892+1008 of SEQID NO:6. Preferably, the xanthan lyase variant has an alterationselected from the group consisting of the following alterations:E229N+N672D+P752K+G753E+A769D+L775A+D801G+K875T+N892Y;E2295+N672D+G753E+5754E+A769D+L775A+D801G+K875T+N892Y;E2295+N672D+P752R+G753E+5754E+A769D+L775A+D801G+K875T+N892Y;A190Q+E229S+1234V+A624E+N672D+G753E+S754E+A769D+L775A+D801G+K875T;A190Q+E229S+T631N+N672D+I703L+P752K+G753E+A769D+L775A+D801G+K875T;A190Q+E229S+1234V+S582K+N672D+G753E+S754E+S757D+A769D+L775A+D801G+K875T+N892Y;

E229S+N440K+S582K+A624E+S635E+N672D+G738L+G753E+S754E+S757D+A769D+L775A+D801G+K875T+N892Y; orS100D+E229S+K360G+D458S+S582K+N672D+G753E+S754E+S757D+A769D+L775A+D801G+A843P+K875T+N892Y+N1008D of SEQ ID NO:6. In a particular aspect, thexanthan lyase variant has besides the afore-mentioned alterations nofurther alterations relative to the parent enzyme of SEQ ID NO:6, i.e.the remaining sequence is identical to SEQ ID NO:6.

In various embodiments, the preferred endoglucanase variants arecombined with the preferred xanthan lyase variants. In some aspects, thedetergent composition thus comprises

(A) an endoglucanase variant selected from those having at least 61%,62%, 63%, 64%, 65%, 66%, 67%, 68%, 69%, 70%, 71%, 72%, 73%, 74%, 75%,76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%,90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, or 98% sequence identity to SEQID NO:2 and having an alteration (e.g. a substitution, deletion orinsertion, preferably a substitution) in the positions selected from thegroup consisting of positions: 559+579+697; 512+559+579+697;18+71+186+408+579+602+651+688+756;18+189+408+559+579+688+697+756+921+934; 313+488; 880+905+921+934;302+313+408+579+602+651+697+880+921+934; or216+313+408+476+579+602+638+651+697+719+880+887+921+934 of SEQ ID NO:2,preferably having an alteration selected from the group consisting ofthe following alterations: (A1) A559N+Y579W+T697G; (A2)K512P+A559N+Y579W+T697G; (A3)N18G+A71E+A186P+E408D+Y579W+I602T+A651P+A688G+V756Y; (A4)N18G+N189K+E408D+A559N+Y579W+A688G+T697G+V756Y+K921R+Y934G; (A5)S313D+E408D; (A6) R880K+N905D+K921R+Y934G; (A7)I302D+S313D+E408D+Y579W+I602T+A651P+T697G+R880K+K921R+Y934G; and (A8)N216Q+S313D+E408D+D476R+Y579W+I602T+F638N+A651P+T697G+W719R+R880K+T887K+K921R+Y934G of SEQ ID NO:2; and

(B) a xanthan lyase variant having at least 61%, 62%, 63%, 64%, 65%,66%, 67%, 68%, 69%, 70%, 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%,80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%,94%, 95%, 96%, 97%, or 98% sequence identity to SEQ ID NO:6 and havingan alteration (e.g. a substitution, deletion or insertion, preferably asubstitution) in the positions selected from the group consisting ofpositions: 229+672+752+753+769+775+801+875+892;229+672+753+754+769+775+801+875+892;229+672+752+753+754+769+775+801+875+892;190+229+234+624+672+753+754+769+775+801+875;190+229+631+672+703+752+753+769+775+801+875;190+229+234+582+672+753+754+757+769+775+801+875+892;229+440+582+624+635+672+738+753+754+757+769+775+801+875+892; or100+229+360+458+582+672+753+754+757+769+775+801+843+875+892+1008 of SEQID NO:6, preferably having an alteration selected from the groupconsisting of the following alterations: (B1)E229N+N672D+P752K+G753E+A769D+L775A+D801G+K875T+N892Y; (B2)E2295+N672D+G753E+5754E+A769D+L775A+D801G+K875T+N892Y; (B3)E229S+N672D+P752R+G753E+S754E+A769D+L775A+D801G+K875T+N892Y; (B4)A190Q+E229S+1234V+A624E+N672D+G753E+S754E+A769D+L775A+D801G+K875T; (B5)A190Q+E229S+T631N+N672D+1703L+P752K+G753E+A769D+L775A+D801G+K875T; (B6)A190Q+E229S+1234V+S582K+N672D+G753E+S754E+S757D+A769D+L775A+D801G+K875T+N892Y; (B7)E229S+N440K+S582K+A624E+S635E+N672D+G738L+G753E+S754E+S757D+A769D+L775A+D801G+K875T+N892Y; and (B8)

S100D+E229S+K360G+D458S+S582K+N672D+G753E+S754E+S757D+A769D+L775A+D801G+A843P+K875T+N892Y+N1008D of SEQ ID NO:6.

In some aspects, the endoglucanase and/or the xanthan lyase variant donot comprise any further substitution besides those explicitly mentionedabove, i.e. the remainder of the sequence is identical to that of theparent enzyme as set forth in SEQ ID NO:2 and SEQ ID NO:6, respectively.

In some aspects, in the detergent compositions of the invention theendoglucanase variant A1, as defined above, can be combined with any oneof the xanthan lyase variants B1, B2, B3, B4, B5, B6, B7 and B8, asdefined above, wherein the respective enzymes preferably have at least90%, preferably at least 91%, 92%, 93%, 94%, 95%, 96% or 97% sequenceidentity to SEQ ID NO:2 and SEQ ID NO:6, respectively, preferably areidentical to their respective parent sequence with the exception of thesubstitutions explicitly mentioned herein.

In some aspects, in the detergent compositions of the invention theendoglucanase variant A2, as defined above, can be combined with any oneof the xanthan lyase variants B1, B2, B3, B4, B5, B6, B7 and B8, asdefined above, wherein the respective enzymes preferably have at least90%, preferably at least 91%, 92%, 93%, 94%, 95%, 96% or 97% sequenceidentity to SEQ ID NO:2 and SEQ ID NO:6, respectively, preferably areidentical to their respective parent sequence with the exception of thesubstitutions explicitly mentioned herein.

In some aspects, in the detergent compositions of the invention theendoglucanase variant A3, as defined above, can be combined with any oneof the xanthan lyase variants B1, B2, B3, B4, B5, B6, B7 and B8, asdefined above, wherein the respective enzymes preferably have at least90%, preferably at least 91%, 92%, 93%, 94%, 95%, 96% or 97% sequenceidentity to SEQ ID NO:2 and SEQ ID NO:6, respectively, preferably areidentical to their respective parent sequence with the exception of thesubstitutions explicitly mentioned herein.

In some aspects, in the detergent compositions of the invention theendoglucanase variant A4, as defined above, can be combined with any oneof the xanthan lyase variants B1, B2, B3, B4, B5, B6, B7 and B8, asdefined above, wherein the respective enzymes preferably have at least90%, preferably at least 91%, 92%, 93%, 94%, 95%, 96% or 97% sequenceidentity to SEQ ID NO:2 and SEQ ID NO:6, respectively, preferably areidentical to their respective parent sequence with the exception of thesubstitutions explicitly mentioned herein.

In some aspects, in the detergent compositions of the invention theendoglucanase variant A5, as defined above, can be combined with any oneof the xanthan lyase variants B1, B2, B3, B4, B5, B6, B7 and B8, asdefined above, wherein the respective enzymes preferably have at least90%, preferably at least 91%, 92%, 93%, 94%, 95%, 96% or 97% sequenceidentity to SEQ ID NO:2 and SEQ ID NO:6, respectively, preferably areidentical to their respective parent sequence with the exception of thesubstitutions explicitly mentioned herein.

In some aspects, in the detergent compositions of the invention theendoglucanase variant A6, as defined above, can be combined with any oneof the xanthan lyase variants B1, B2, B3, B4, B5, B6, B7 and B8, asdefined above, wherein the respective enzymes preferably have at least90%, preferably at least 91%, 92%, 93%, 94%, 95%, 96% or 97% sequenceidentity to SEQ ID NO:2 and SEQ ID NO:6, respectively, preferably areidentical to their respective parent sequence with the exception of thesubstitutions explicitly mentioned herein.

In some aspects, in the detergent compositions of the invention theendoglucanase variant A7, as defined above, can be combined with any oneof the xanthan lyase variants B1, B2, B3, B4, B5, B6, B7 and B8, asdefined above, wherein the respective enzymes preferably have at least90%, preferably at least 91%, 92%, 93%, 94%, 95%, 96% or 97% sequenceidentity to SEQ ID NO:2 and SEQ ID NO:6, respectively, preferably areidentical to their respective parent sequence with the exception of thesubstitutions explicitly mentioned herein.

In some aspects, in the detergent compositions of the invention theendoglucanase variant A8, as defined above, can be combined with any oneof the xanthan lyase variants B1, B2, B3, B4, B5, B6, B7 and B8, asdefined above, wherein the respective enzymes preferably have at least90%, preferably at least 91%, 92%, 93%, 94%, 95%, 96% or 97% sequenceidentity to SEQ ID NO:2 and SEQ ID NO:6, respectively, preferably areidentical to their respective parent sequence with the exception of thesubstitutions explicitly mentioned herein.

In some aspects, preferred combinations of endoglucanase variants andxanthan lyase variants of the invention, are the combinations disclosedin Tables 34-36.

In some aspects, the present invention relates to a detergentcomposition additionally comprising one or more further detergentcomponents, preferably a surfactant.

In some aspects, the present invention relates to use of a compositionof the present invention, wherein said use is selected from the groupconsisting of: use for degrading xanthan gum and use in a cleaningprocess, such as laundry or hard surface cleaning such as dish wash.

In some aspects, the present invention further relates to the use of adetergent composition of the invention for degrading xanthan gum, forwashing or cleaning textiles and/or hard surfaces, such as dish wash,wherein the composition has an enzyme detergency benefit.

In some aspects, the present invention also relates to methods ofdegrading xanthan gum using detergent compositions of the presentinvention, wherein xanthan gum is on the surface of a hard surface ortextile.

OVERVIEW OF SEQUENCE LISTING

SEQ ID NO:1 is the DNA sequence of the parent mature endoglucanase froma strain of a Paenibacillus sp.

SEQ ID NO:2 is the amino acid sequence of mature polypeptide encoded bySEQ ID NO:1.

SEQ ID NO:3 is the DNA sequence of the alpha-amylase secretion signalfrom Bacillus licheniformis.

SEQ ID NO:4 is the amino acid sequence of the alpha-amylase secretionsignal from Bacillus licheniformis.

SEQ ID NO:5 is the DNA sequence of the parent mature xanthan lyase froma strain of a Paenibacillus sp.

SEQ ID NO:6 is the amino acid sequence of the mature polypeptide encodedby SEQ ID NO:5.

Definitions

cDNA: The term “cDNA” means a DNA molecule that can be prepared byreverse transcription from a mature, spliced, mRNA molecule obtainedfrom a eukaryotic or prokaryotic cell. cDNA lacks intron sequences thatmay be present in the corresponding genomic DNA. The initial, primaryRNA transcript is a precursor to mRNA that is processed through a seriesof steps, including splicing, before appearing as mature spliced mRNA.

Cleaning or Detergent Application: the term “cleaning or detergentapplication” means applying the endoglucanase of the application in anycomposition for the purpose of cleaning or washing, by hand, machine orautomated, a hard surface or a textile.

Cleaning Composition: the term “cleaning composition” refers tocompositions that find use in the removal of undesired compounds fromitems to be cleaned, such as textiles, dishes, and hard surfaces. Theterms encompass any materials/compounds selected for the particular typeof cleaning composition desired and the form of the product (e.g.liquid, gel, powder, granulate, paste, or spray compositions) andincludes, but is not limited to, detergent compositions (e.g. liquidand/or solid laundry detergents and fine fabric detergents; hard surfacecleaning formulations, such as for glass, wood, ceramic and metalcounter tops and windows; carpet cleaners; oven cleaners; fabricfresheners; fabric softeners; and textile and laundry pre-spotters, aswell as dish wash detergents). In addition to the endoglucanase andxanthan lyase, the detergent formulation may contain one or moreadditional enzymes (such as xanthan lyases, proteases, amylases,lipases, cutinases, cellulases, endoglucanases, xyloglucanases,pectinases, pectin lyases, xanthanases, peroxidaes, haloperoxygenases,catalases and mannanases, or any mixture thereof), and/or componentssuch as surfactants, builders, chelators or chelating agents, bleachsystem or bleach components, polymers, fabric conditioners, foamboosters, suds suppressors, dyes, perfume, tannish inhibitors, opticalbrighteners, bactericides, fungicides, soil suspending agents, anticorrosion agents, enzyme inhibitors or stabilizers, enzyme activators,transferase(s), hydrolytic enzymes, oxido reductases, bluing agents andfluorescent dyes, antioxidants, and solubilizers.

Coding sequence: The term “coding sequence” means a polynucleotide,which directly specifies the amino acid sequence of a polypeptide. Theboundaries of the coding sequence are generally determined by an openreading frame, which begins with a start codon such as ATG, GTG, or TTGand ends with a stop codon such as TAA, TAG, or TGA. The coding sequencemay be a genomic DNA, cDNA, synthetic DNA, or a combination thereof.

Colour clarification: During washing and wearing loose or broken fiberscan accumulate on the surface of the fabrics. One consequence can bethat the colours of the fabric appear less bright or less intensebecause of the surface contaminations. Removal of the loose or brokenfibers from the textile will partly restore the original colours andlooks of the textile. By the term “colour clarification”, as usedherein, is meant the partial restoration of the initial colours oftextile.

Control sequences: The term “control sequences” means nucleic acidsequences necessary for expression of a polynucleotide encoding a maturepolypeptide of the present invention. Each control sequence may benative (i.e. from the same gene) or foreign (i.e. from a different gene)to the polynucleotide encoding the polypeptide or native or foreign toeach other. Such control sequences include, but are not limited to, aleader, polyadenylation sequence, propeptide sequence, promoter, signalpeptide sequence, and transcription terminator. At a minimum, thecontrol sequences include a promoter, and transcriptional andtranslational stop signals. The control sequences may be provided withlinkers for the purpose of introducing specific restriction sitesfacilitating ligation of the control sequences with the coding region ofthe polynucleotide encoding a polypeptide.

Corresponding to: The term “corresponding to” as used herein, refers toa way of determining the specific amino acid of a sequence whereinreference is made to a specific amino acid sequence. E.g. for thepurposes of the present invention, when references are made to specificamino acid positions, the skilled person would be able to align anotheramino acid sequence to said amino acid sequence that reference has beenmade to, in order to determine which specific amino acid may be ofinterest in said another amino acid sequence. Alignment of another aminoacid sequence with e.g. the sequence as set forth in SEQ ID NO:2, or anyother amino acid sequence listed herein, has been described elsewhereherein. Alternative alignment methods may be used, and are well-knownfor the skilled person.

Degrading xanthan gum and xanthan gum degrading activity: The terms“degrading xanthan gum” and “xanthan gum degrading activity” are usedinterchangebly and are defined as the depolymerization, degradation orbreaking down of xanthan gum into smaller components. The degradation ofxanthan gum can either be the removal of one or more side chainsaccharides, the cutting of the backbone of xanthan gum into smallercomponents or the removal of one or more side chain saccharides and thecutting of the backbone of xanthan gum into smaller components. Apreferred assay for measuring degradation of xanthan gum is the reducingsugar assay as described in Examples 3 and 7 herein. Non-limitingexamples of the xanthan gum degrading activity include endoglucanase EC3.2.1.4 activity and/or xanthan lyase EC 4.2.2.12 activity.

Detergent component: the term “detergent component” is defined herein tomean the types of chemicals which can be used in detergent compositions.Examples of detergent components are surfactants, hydrotropes, builders,co-builders, chelators or chelating agents, bleaching system or bleachcomponents, polymers, fabric hueing agents, fabric conditioners, foamboosters, suds suppressors, dispersants, dye transfer inhibitors,fluorescent whitening agents, perfume, optical brighteners,bactericides, fungicides, soil suspending agents, soil release polymers,anti-redeposition agents, enzyme inhibitors or stabilizers, enzymeactivators, antioxidants, and solubilizers. The detergent compositionmay comprise of one or more of any type of detergent component.

Detergent composition: the term “detergent composition” refers tocompositions that find use in the removal of undesired compounds fromitems to be cleaned, such as textiles, dishes, and hard surfaces. Thedetergent composition may be used to e.g. clean textiles, dishes andhard surfaces for both household cleaning and industrial cleaning. Theterms encompass any materials/compounds selected for the particular typeof cleaning composition desired and the form of the product (e.g.liquid, gel, powder, granulate, paste, or spray compositions) andincludes, but is not limited to, detergent compositions (e.g. liquidand/or solid laundry detergents and fine fabric detergents; hard surfacecleaning formulations, such as for glass, wood, ceramic and metalcounter tops and windows; carpet cleaners; oven cleaners; fabricfresheners; fabric softeners; and textile and laundry pre-spotters, aswell as dish wash detergents). In addition to containing a GH9endoglucanase as described herein and/or xanthan lyase, the detergentformulation may contain one or more additional enzymes (such as xanthanlyases, proteases, amylases, lipases, cutinases, cellulases,endoglucanases, xyloglucanases, pectinases, pectin lyases, xanthanases,peroxidaes, haloperoxygenases, catalases and mannanases, or any mixturethereof), and/or components such as surfactants, builders, chelators orchelating agents, bleach system or bleach components, polymers, fabricconditioners, foam boosters, suds suppressors, dyes, perfume, tannishinhibitors, optical brighteners, bactericides, fungicides, soilsuspending agents, anti corrosion agents, enzyme inhibitors orstabilizers, enzyme activators, transferase(s), hydrolytic enzymes,oxido reductases, bluing agents and fluorescent dyes, antioxidants, andsolubilizers.

Dish wash: The term “dish wash” refers to all forms of washing dishes,e.g. by hand or automatic dish wash. Washing dishes includes, but is notlimited to, the cleaning of all forms of crockery such as plates, cups,glasses, bowls, all forms of cutlery such as spoons, knives, forks andserving utensils as well as ceramics, plastics, metals, china, glass andacrylics.

Dish washing composition: The term “dish washing composition” refers toall forms of compositions for cleaning hard surfaces. The presentinvention is not restricted to any particular type of dish washcomposition or any particular detergent.

Endoglucanase: The term “endoglucanase” or “EG” means anendo-1,4-(1,3;1,4)-beta-D-glucan 4-glucanohydrolase (EC 3.2.1.4) thatcatalyzes endohydrolysis of 1,4-beta-D-glycosidic linkages in cellulose,cellulose derivatives (such as carboxymethyl cellulose and hydroxyethylcellulose), lichenin, beta-1,4 bonds in mixed beta-1,3 glucans such ascereal beta-D-glucans, xyloglucans, xanthans and other plant materialcontaining cellulosic components. Endoglucanase activity can bedetermined by measuring reduction in substrate viscosity or increase inreducing ends determined by a reducing sugar assay (Zhang et al., 2006,Biotechnology Advances 24: 452-481). A preferred assay for measuringendoglucanase activity is the reducing sugar assay as described inExamples 3 and 7 herein. Non-limiting examples of endoglucanases includethe mature parent endoglucanase having SEQ ID NO:2.

Enzyme detergency benefit: The term “enzyme detergency benefit” isdefined herein as the advantageous effect an enzyme may add to adetergent compared to the same detergent without the enzyme. Importantdetergency benefits which can be provided by enzymes are stain removalwith no or very little visible soils after washing and or cleaning,prevention or reduction of redeposition of soils released in the washingprocess an effect that also is termed anti-redeposition, restoring fullyor partly the whiteness of textiles, which originally were white butafter repeated use and wash have obtained a greyish or yellowishappearance an effect that also is termed whitening. Textile carebenefits, which are not directly related to catalytic stain removal orprevention of redeposition of soils are also important for enzymedetergency benefits. Examples of such textile care benefits areprevention or reduction of dye transfer from one fabric to anotherfabric or another part of the same fabric an effect that is also termeddye transfer inhibition or anti-backstaining, removal of protruding orbroken fibers from a fabric surface to decrease pilling tendencies orremove already existing pills or fuzz an effect that also is termedanti-pilling, improvement of the fabric-softness, colour clarificationof the fabric and removal of particulate soils which are trapped in thefibers of the fabric or garment. Enzymatic bleaching is a further enzymedetergency benefit where the catalytic activity generally is used tocatalyze the formation of bleaching component such as hydrogen peroxideor other peroxides.

Expression: The term “expression” includes any step involved in theproduction of a polypeptide including, but not limited to,transcription, post-transcriptional modification, translation,post-translational modification, and secretion.

Expression vector: The term “expression vector” means a linear orcircular DNA molecule that comprises a polynucleotide encoding apolypeptide and is operably linked to control sequences that provide forits expression.

Fragment: The term “fragment” means a polypeptide having one or more(e.g. several) amino acids absent from the amino and/or carboxylterminus of a mature polypeptide; wherein the fragment has endoglucanaseactivity. In one aspect, a fragment contains at least 85%, 86%, 87%,88%, 89%, 90%, 91%, 92%, 93%, 94% or 95% of the number of amino acids ofthe mature polypeptide.

Endoglucanase variant having activity on xanthan gum pretreated withxanthan lyase: The term “Endoglucanase variant having activity onxanthan gum pretreated with xanthan lyase” or an “endoglucanase havingactivity on xanthan gum pretreated with xanthan lyase and belonging tothe GH9 class of glycosyl hydrioases” is defined as a polypeptidecomprising a domain belonging to the GH9 class of glycosyl hydrolases,and having activity (e.g. enzymatic activity, xanthan degradingactivity, endoglucanase EC 3.2.1.4 activity) on xanthan gum pretreatedwith xanthan lyase. A preferred assay for measuring activity on xanthangum pretreated with xanthan lyase is disclosed in Example 3 herein.

Xanthan lyase variant having activity on xanthan gum: The term “Xanthanlyase variant having activity on xanthan gum” is defined as apolypeptide that cleaves the □-D-mannosyl-□-D-1,4-glucuronosyl bond ofxanthan (e.g. xanthan lyase EC 4.2.2.12 activity). A preferred assay formeasuring activity on xanthan gum is disclosed in Example 7 herein.Examples of the xanthan lyase variants having activity on xanthan gum,are xanthan lyase polypeptides as such. Thus, polypeptides that thatcleaves the □-D-mannosyl-□-D-1,4-glucuronosyl bond of xanthan.

Half-life: the term “half-life” is the time it takes for an enzyme tolose half of its enzymatic activity under a given set of conditions. Itis denoted as T_(1/2) and is measured in hours (h). Half-lifes can becalculated at a given detegent concentration and storage temperature fora Wild-type control and/or variants, as the degradation follows anexponential decay and the incubation time (hours) is known, i.e.according to the following formulas:

T½ (variant)=(Ln (0.5)/Ln (RA-variant/100))*Time

T½ (wild-type)=(Ln (0.5)/Ln (RA-wild-type/100))*Time

Wherein ‘RA’ is the residual activity in percent and ‘Time’ is theincubation time in hours.

Half-life improvement factor: the term “Half-life improvement factor” or“HIF” is the improvement of half-life of a variant compared to theparent polypeptide, such as the parent endoglucanase. A half-lifeimprovement factor (HIF) under a given set of storage conditions(detergent concentration and temperature) can be calculated as: HIF=T½(variant)/T½ (wild-type) where the wild-type (wt) is incubated under thesame storage condition (detergent concentration and incubationtemperature) as the variant. In the cases where the difference instability between wild-type and variant is too big to accurately assesshalf-life for both wild-type and variant using the same incubation time,the incubation time for wild-type and variant is different e.g. 1 h forwild-type and >100 h for more stable variants. The half-life improvementfactor may also be calculated based on the half-life of a parent xanthanlyase (see the definition of “parent” below) that is not necessarily awild-type. Preferred ways of calculating HIF are also described inExamples 3 and 7 herein.

Hard surface cleaning: The term “Hard surface cleaning” is definedherein as cleaning of hard surfaces wherein hard surfaces may includefloors, tables, walls, roofs etc. as well as surfaces of hard objectssuch as cars (car wash) and dishes (dish wash). Dish washing includesbut are not limited to cleaning of plates, cups, glasses, bowls, andcutlery such as spoons, knives, forks, serving utensils, ceramics,plastics, metals, china, glass and acrylics.

Host cell: The term “host cell” means any cell type that is susceptibleto transformation, transfection, transduction, or the like with anucleic acid construct or expression vector comprising a polynucleotideof the present invention. The term “host cell” encompasses any progenyof a parent cell that is not identical to the parent cell due tomutations that occur during replication.

Improved property: The term “improved property” means a characteristicassociated with a variant that is improved compared to the parent. Suchimproved properties include, but are not limited to, catalyticefficiency, catalytic rate, chemical stability, oxidation stability, pHactivity, pH stability, specific activity, stability under storageconditions, chelator stability, substrate binding, substrate cleavage,substrate specificity, substrate stability, surface properties, thermalactivity, and thermostability.

Improved wash performance: The term “improved wash performance” isdefined herein as a (variant) enzyme (also a blend of enzymes, notnecessarily only variants but also backbones, and in combination withcertain cleaning composition etc.) displaying an alteration of the washperformance of a protease variant relative to the wash performance ofthe parent protease variant e.g. by increased stain removal. The term“wash performance” includes wash performance in laundry but also e.g. indish wash.

Isolated: The term “isolated” means a substance in a form or environmentthat does not occur in nature. Non-limiting examples of isolatedsubstances include (1) any non-naturally occurring substance, (2) anysubstance including, but not limited to, any enzyme, variant, nucleicacid, protein, peptide or cofactor, that is at least partially removedfrom one or more or all of the naturally occurring constituents withwhich it is associated in nature; (3) any substance modified by the handof man relative to that substance found in nature; or (4) any substancemodified by increasing the amount of the substance relative to othercomponents with which it is naturally associated (e.g. multiple copiesof a gene encoding the substance; use of a stronger promoter than thepromoter naturally associated with the gene encoding the substance). Anisolated substance may be present in a fermentation broth sample.

Laundering: The term “laundering” relates to both household launderingand industrial laundering and means the process of treating textileswith a solution containing a cleaning or detergent composition of thepresent invention. The laundering process can for example be carried outusing e.g. a household or an industrial washing machine or can becarried out by hand.

Mature polypeptide: The term “mature polypeptide” means a polypeptide inits final form following translation and any post-translationalmodifications, such as N-terminal processing, C-terminal truncation,glycosylation, phosphorylation, etc. In one aspect, the maturepolypeptide is amino acids 1 to 1055 of SEQ ID NO:2 or amino acids 1 to1037 of SEQ ID NO:6.

It is known in the art that a host cell may produce a mixture of two ofmore different mature polypeptides (i.e. with a different C-terminaland/or N-terminal amino acid) expressed by the same polynucleotide. Itis also known in the art that different host cells process polypeptidesdifferently, and thus, one host cell expressing a polynucleotide mayproduce a different mature polypeptide (e.g. having a differentC-terminal and/or N-terminal amino acid) as compared to another hostcell expressing the same polynucleotide.

Mature polypeptide coding sequence: The term “mature polypeptide codingsequence” means a polynucleotide that encodes a mature polypeptidehaving enzymatic activity such as activity on xanthan gum pretreatedwith xanthan lyase or xanthan lyase activity. In one aspect, the maturepolypeptide coding sequence is nucleotides 1 to 3165 of SEQ ID NO:1 ornucleotides 1 to 3111 of SEQ ID NO:5.

Mutant: The term “mutant” means a polynucleotide encoding a variant.

Nucleic acid construct: The term “nucleic acid construct” means anucleic acid molecule, either single- or double-stranded, which isisolated from a naturally occurring gene or is modified to containsegments of nucleic acids in a manner that would not otherwise exist innature or which is synthetic, which comprises one or more controlsequences.

Operably linked: The term “operably linked” means a configuration inwhich a control sequence is placed at an appropriate position relativeto the coding sequence of a polynucleotide such that the controlsequence directs expression of the coding sequence.

Parent: The term “parent” or “parent endoglucanase” means anypolypeptide with endoglucanase activity to which an alteration is madeto produce the enzyme variants of the present invention. In one aspect,the parent is an endoglucanase having the identical amino acid sequenceof the variant, but not having the alterations at one or more of thespecified positions. It will be understood, that the expression “havingidentical amino acid sequence” relates to 100% sequence identity.Non-limiting examples of parent endoglucanases include the mature parentendoglucanase having SEQ ID NO:2.

Sequence identity: The relatedness between two amino acid sequences orbetween two nucleotide sequences is described by the parameter “sequenceidentity”. For purposes of the present invention, the sequence identitybetween two amino acid sequences is determined using theNeedleman-Wunsch algorithm (Needleman and Wunsch, 1970, J. Mol. Biol.48: 443-453) as implemented in the Needle program of the EMBOSS package(EMBOSS: The European Molecular Biology Open Software Suite, Rice etal., 2000, Trends Genet. 16: 276-277), preferably version 5.0.0 orlater. The parameters used are gap open penalty of 10, gap extensionpenalty of 0.5, and the EBLOSUM62 (EMBOSS version of BLOSUM62)substitution matrix. The output of Needle labeled “longest identity”(obtained using the—nobrief option) is used as the percent identity andis calculated as follows:

(Identical Residues×100)/(Length of Alignment—Total Number of Gaps inAlignment)

For purposes of the present invention, the sequence identity between twodeoxyribonucleotide sequences is determined using the Needleman-Wunschalgorithm (Needleman and Wunsch, 1970, supra) as implemented in theNeedle program of the EMBOSS package (EMBOSS: The European MolecularBiology Open Software Suite, Rice et al., 2000, supra), preferablyversion 5.0.0 or later. The parameters used are gap open penalty of 10,gap extension penalty of 0.5, and the EDNAFULL (EMBOSS version of NCBINUC4.4) substitution matrix. The output of Needle labeled “longestidentity” (obtained using the—nobrief option) is used as the percentidentity and is calculated as follows:

(Identical Deoxyribonucleotides x 100)/(Length of Alignment−Total Numberof Gaps in Alignment)

Stringency conditions: The different strigency conditions are defined asfollows.

The term “very low stringency conditions” means for probes of at least100 nucleotides in length, prehybridization and hybridization at 42° C.in 5× SSPE, 0.3% SDS, 200 mg/mL sheared and denatured salmon sperm DNA,and 25% formamide, following standard Southern blotting procedures for12 to 24 h. The carrier material is finally washed three times each for15 minutes using 2× SSC, 0.2% SDS at 45° C.

The term “low stringency conditions” means for probes of at least 100nucleotides in length, prehybridization and hybridization at 42° C. in5× SSPE, 0.3% SDS, 200 mg/mL sheared and denatured salmon sperm DNA, and25% formamide, following standard Southern blotting procedures for 12 to24 h. The carrier material is finally washed three times each for 15minutes using 2× SSC, 0.2% SDS at 50° C.

The term “medium stringency conditions” means for probes of at least 100nucleotides in length, prehybridization and hybridization at 42° C. in5× SSPE, 0.3% SDS, 200 mg/mL sheared and denatured salmon sperm DNA, and35% formamide, following standard Southern blotting procedures for 12 to24 h. The carrier material is finally washed three times each for 15minutes using 2× SSC, 0.2% SDS at 55° C.

The term “medium-high stringency conditions” means for probes of atleast 100 nucleotides in length, prehybridization and hybridization at42° C. in 5× SSPE, 0.3% SDS, 200 mg/mL sheared and denatured salmonsperm DNA, and 35% formamide, following standard Southern blottingprocedures for 12 to 24 h. The carrier material is finally washed threetimes each for 15 minutes using 2× SSC, 0.2% SDS at 60° C.

The term “high stringency conditions” means for probes of at least 100nucleotides in length, prehybridization and hybridization at 42° C. in5× SSPE, 0.3% SDS, 200 mg/mL sheared and denatured salmon sperm DNA, and50% formamide, following standard Southern blotting procedures for 12 to24 h. The carrier material is finally washed three times each for 15minutes using 2× SSC, 0.2% SDS at 65° C.

The term “very high stringency conditions” means for probes of at least100 nucleotides in length, prehybridization and hybridization at 42° C.in 5× SSPE, 0.3% SDS, 200 mg/mL sheared and denatured salmon sperm DNA,and 50% formamide, following standard Southern blotting procedures for12 to 24 h. The carrier material is finally washed three times each for15 minutes using 2× SSC, 0.2% SDS at 70° C.

Subsequence: The term “subsequence” means a polynucleotide having one ormore (e.g. several) nucleotides absent from the 5′ and/or 3′ end of amature polypeptide coding sequence; wherein the subsequence encodes afragment having enzymatic activity, such as activity on xanthan gumpretreated with xanthan lyase or xanthan lyase activity.

Textile: The term “textile” means any textile material including yarns,yarn intermediates, fibers, non-woven materials, natural materials,synthetic materials, and any other textile material, fabrics made ofthese materials and products made from fabrics (e.g. garments and otherarticles). The textile or fabric may be in the form of knits, wovens,denims, non-wovens, felts, yarns, and towelling. The textile may becellulose based such as natural cellulosics, including cotton,flax/linen, jute, ramie, sisal or coir or manmade cellulosics (e.g.originating from wood pulp) including viscose/rayon, ramie, celluloseacetate fibers (tricell), lyocell or blends thereof. The textile orfabric may also be non-cellulose based such as natural polyamidesincluding wool, camel, cashmere, mohair, rabit and silk or syntheticpolymer such as nylon, aramid, polyester, acrylic, polypropylen andspandex/elastane, or blends thereof as well as blend of cellulose basedand non-cellulose based fibers. Examples of blends are blends of cottonand/or rayon/viscose with one or more companion material such as wool,synthetic fibers (e.g. polyamide fibers, acrylic fibers, polyesterfibers, polyvinyl alcohol fibers, polyvinyl chloride fibers,polyurethane fibers, polyurea fibers, aramid fibers), andcellulose-containing fibers (e.g. rayon/viscose, ramie, flax/linen,jute, cellulose acetate fibers, lyocell). Fabric may be conventionalwashable laundry, for example stained household laundry. When the termfabric or garment is used it is intended to include the broader termtextiles as well.

Textile care benefit: “Textile care benefits”, which are not directlyrelated to catalytic stain removal or prevention of redeposition ofsoils, are also important for enzyme detergency benefits. Examples ofsuch textile care benefits are prevention or reduction of dye transferfrom one textile to another textile or another part of the same textilean effect that is also termed dye transfer inhibition oranti-backstaining, removal of protruding or broken fibers from a textilesurface to decrease pilling tendencies or remove already existing pillsor fuzz an effect that also is termed anti-pilling, improvement of thetextile-softness, colour clarification of the textile and removal ofparticulate soils which are trapped in the fibers of the textile.Enzymatic bleaching is a further enzyme detergency benefit where thecatalytic activity generally is used to catalyze the formation ofbleaching component such as hydrogen peroxide or other peroxides orother bleaching species.

Variant: The term “variant” means a polypeptide (e.g. a GH9endoglucanase polypeptide) comprising an alteration i.e. a substitution,insertion, and/or deletion, at one or more (e.g. several) positions. Asubstitution means replacement of the amino acid occupying a positionwith a different amino acid; a deletion means removal of the amino acidoccupying a position; and an insertion means adding one or more (e.g.several) amino acids e.g. 1-5 amino acids adjacent to and immediatelyfollowing the amino acid occupying a position. Non-limiting examples ofendoglucanase/xanthan lyase variants described herein includeendoglucanase/xanthan lyase variants having an activity on xanthan gum(for xanthan lyase) and xanthan gum pretreated with xanthan lyase (forendoglucanase). Non-limiting examples of variants escribed hereinfurther include variants having at least 20%, e.g. at least 40%, 50%,60%, 70%, 80%, 90%, 95%, or 100% endoglucanase activity of the matureparent having SEQ ID NO:2 or SEQ ID NO:6. A preferred assay formeasuring activity on xanthan gum (optionally pretreated with xanthanlyase) is disclosed in Examples 3 and 7 herein.

Stability: The term “stability” means resistance or the degree ofresistance to change, unfolding, disintegration, denaturation oractivity loss. Non-limiting examples of stability include conformationalstability, storage stability and stability during use, e.g. during awash process and reflects the stability of a polypeptide (e.g. anendoglucanase or xanthan lyase variant according to the invention) as afunction of time, e.g. how much activity is retained when saidpolypeptide (e.g. said endoglucanase or xanthan lyase variant) is keptin solution, in particular in a detergent solution. The stability isinfluenced by many factors, e.g. presence of chelator(s), pH,temperature, detergent composition, e.g. amount of builders,surfactants, chelators etc. The endoglucanase or xanthan lyase stabilitymay be measured using a half-life improvement factor (HIF) as describedin Examples 3 and 7 herein, e.g. relative to the parent enzyme havingSEQ ID NO:2 or 6. The endoglucanase stability may also be measured usinga reducing sugar assay as described in Example 3 herein.

Improved stability: The term “improved stability” or “increasedstability” is defined herein as increased stability in a detergentcomposition (e.g. in solutions, e.g. in the presence of a chelator, e.g.EDTA or citrate), relative to the stability of the parentendoglucanase/xanthan lyase, relative to an endoglucanase/xanthan lyasehaving the identical amino acid sequence of the variant, but not havingthe alterations at one or more of the specified positions, or relativeto SEQ ID NO:2 and SEQ ID NO:6, respectively. The terms “improvedstability” and “increased stability” includes “improved chemicalstability”, “detergent stability” and “improved detergent stability.

Improved chemical stability: The term “improved chemical stability” isdefined herein as a variant enzyme displaying retention of enzymaticactivity after a period of incubation in the presence of a chemical orchemicals, either naturally occurring or synthetic, which reduces theenzymatic activity of the parent enzyme. Improved chemical stability mayalso result in variants being more able (e.g. better that the parent) tocatalyze a reaction in the presence of such chemicals. In a particularaspect of the invention the improved chemical stability is an improvedstability in a detergent, in particular in a liquid detergent. The term“detergent stability” or “improved detergent stability is in particularan improved stability of the endoglucanase/xanthan lyase compared to theparent endoglucanase/xanthan lyase, when an endoglucanasevariant/xanthan lyase variant of the present invention is mixed into aliquid detergent formulation, especially into a liquid detergentformulation comprising a chelator (e.g. EDTA or citrate).

Conformational stability: The term “conformational stability” meansresistance or a degree of resistance to conformational change, unfoldingor disintegration. Accordingly, the term “less conformationally stable”means less resistant or having lesser degree of resistance toconformational change, unfolding or disintegration.

Instability: The term “instability” means lack of stability.Non-limiting examples of instability include conformational instability,unfolding, denaturation, desintegration, activity loss.

Chelator-induced instability region: The term “chelator-inducedinstability region” means any region of a polypeptide contibuting toinstability of said polypeptide in the presence of a chelator.Non-limiting examples of chelators include EDTA(Ethylenediaminetetraacetic acid) and citrate. Non-limiting examples ofchelator-induced instability regions include any region of a polypeptidehaving one or more of the following features: in the presence of achelator it is less conformationally stable than one or more or all ofits adjacent regions; and/or in the presence of a chelator it is moreexposed to said chelator than one or more or all of its adjacentregions; and/or in the presence of a chelator it is more accessible tosaid chelator than one or more or all of its adjacent regions; and/or inthe presence of a chelator it is more conformationally dynamic than oneor more or all of its adjacent regions; and/or in the presence of achelator it is more receptive to deuterium incorporation than one ormore or all of its adjacent regions. Non-limiting examples ofchelator-induced instability regions further include any region of apolypeptide responsible for chelator-induced instability. Non-limitingexamples of chelator-induced instability regions of a matureendoglucanase (e.g. having SEQ ID NO:2) or mature xanthan lyase (e.g.having SEQ ID NO:6) include the regions described above.

Adjacent region: The term “adjacent region” means any region of apolypeptide that is not a chelator-induced instability region.Non-limiting examples of adjacent regions of a mature endoglucanase(e.g. having SEQ ID NO:2) or mature xanthan lyase (e.g. having SEQ IDNO:6) hae been disclosed above.

Chelator exposure: The term “chelator exposure” means concentration oramount of a chelator that reaches a polypeptide. Accordingly, in thecontext of the present invention the term “more exposed to a chelator”means that chelator exposure of a particular region (e.g. achelator-induced instability region) is greater than a chelator exposureof a different region (e.g. an adjacent region). In one aspect, chelatorexposure can be expressed in numerical terms of concentration, duration,and frequency (e.g. for chemical agents, e.g. chelators) or intensity.

Chelator accessibility: The term “chelator accessibility” encompasesopenness to the influence by a chelator and easiness of approach bychelator. Accordingly, in the context of the present invention the term“more accessible to a chelator” means that chelator accessibility of aparticular region (e.g. a chelator-induced instability region) isgreater than a chelator accessibility of a different region (e.g. anadjacent region).

Conformational dynamics: The term “conformational dynamics” encompassesvibrations, structural rearrangements and transitions of a polypeptide(e.g. in solution). Accordingly, in the context of the present inventionthe term “more conformationally dynamic” means that conformationaldynamics of a particular region (e.g. a chelator-induced instabilityregion) is greater than conformational dynamics of a different region(e.g. an adjacent region).

Receptiveness to deuterium incorporation: The term “receptiveness todeuterium incorporation” means amount of hydrogen atoms replaced by adeuterium atoms during hydrogen-deuterium exchange. Said amount can bemeasured in relative (e.g. compared to another amount) or absolute (e.g.expressed numerically) terms. Accordingly, in the context of the presentinvention the term “more receptive to deuterium incorporation” meansthat receptiveness to deuterium incorporation of a particular region(e.g. a chelator-induced instability region) is greater thanreceptiveness to deuterium incorporation of a different region (e.g. anadjacent region).

Wash performance: The term “wash performance” is used as an enzyme'sability to remove stains present on the object to be cleaned during e.g.wash or hard surface cleaning. The improvement in the wash performancemay be quantified by calculating the so-called intensity value (Int) in‘Automatic Mechanical Stress Assay (AMSA) for laundry’ or the remissionvalue (Rem).

Conventions for Designation of Variants

For purposes of the present invention, the mature polypeptide disclosedin SEQ ID NO:2 is used to determine the corresponding amino acid residuein another endoglucanase and the mature polypeptide disclosed in SEQ IDNO:6 is used to determine the corresponding amino acid residue inanother xanthan lyase. The amino acid sequence of anotherendoglucanase/xanthan lyase is aligned with the mature polypeptidedisclosed in SEQ ID NO:2 or SEQ ID NO:6, and based on the alignment, theamino acid position number corresponding to any amino acid residue inthe mature polypeptide disclosed in SEQ ID NO:2 or 6 is determined usingthe Needleman-Wunsch algorithm (Needleman and Wunsch, 1970, J. Mol.Biol. 48: 443-453) as implemented in the Needle program of the EMBOSSpackage (EMBOSS: The European Molecular Biology Open Software Suite,Rice et al., 2000, Trends Genet. 16: 276-277), preferably version 5.0.0or later. The parameters used are gap open penalty of 10, gap extensionpenalty of 0.5, and the EBLOSUM62 (EMBOSS version of BLOSUM62)substitution matrix.

Identification of the corresponding amino acid residue in anotherendoglucanase/xanthan lyase can be determined by an alignment ofmultiple polypeptide sequences using several computer programsincluding, but not limited to, MUSCLE (multiple sequence comparison bylog-expectation; version 3.5 or later; Edgar, 2004, Nucleic AcidsResearch 32: 1792-1797), MAFFT (version 6.857 or later; Katoh and Kuma,2002, Nucleic Acids Research 30: 3059-3066; Katoh et al., 2005, NucleicAcids Research 33: 511-518; Katoh and Toh, 2007, Bioinformatics 23:372-374; Katoh et al., 2009, Methods in Molecular Biology 537:39-64;Katoh and Toh, 2010, Bioinformatics 26:_1899-1900), and EMBOSS EMMAemploying ClustalW (1.83 or later; Thompson et al., 1994, Nucleic AcidsResearch 22: 4673-4680), using their respective default parameters.

When the other enzyme has diverged from the mature polypeptide of SEQ IDNO:2 or 6 such that traditional sequence-based comparison fails todetect their relationship (Lindahl and Elofsson, 2000, J. Mol. Biol.295: 613-615), other pairwise sequence comparison algorithms can beused. Greater sensitivity in sequence-based searching can be attainedusing search programs that utilize probabilistic representations ofpolypeptide families (profiles) to search databases. For example, thePSI-BLAST program generates profiles through an iterative databasesearch process and is capable of detecting remote homologs (Atschul etal., 1997, Nucleic Acids Res. 25: 3389-3402). Even greater sensitivitycan be achieved if the family or superfamily for the polypeptide has oneor more representatives in the protein structure databases. Programssuch as GenTHREADER (Jones, 1999, J. Mol. Biol. 287: 797-815; McGuffinand Jones, 2003, Bioinformatics 19: 874-881) utilize information from avariety of sources (PSI-BLAST, secondary structure prediction,structural alignment profiles, and solvation potentials) as input to aneural network that predicts the structural fold for a query sequence.Similarly, the method of Gough et al., 2000, J. Mol. Biol. 313: 903-919,can be used to align a sequence of unknown structure with thesuperfamily models present in the SCOP database. These alignments can inturn be used to generate homology models for the polypeptide, and suchmodels can be assessed for accuracy using a variety of tools developedfor that purpose.

For proteins of known structure, several tools and resources areavailable for retrieving and generating structural alignments. Forexample, the SCOP superfamilies of proteins have been structurallyaligned, and those alignments are accessible and downloadable. Two ormore protein structures can be aligned using a variety of algorithmssuch as the distance alignment matrix (Holm and Sander, 1998, Proteins33: 88-96) or combinatorial extension (Shindyalov and Bourne, 1998,Protein Engineering 11: 739-747), and implementation of these algorithmscan additionally be utilized to query structure databases with astructure of interest in order to discover possible structural homologs(e.g. Holm and Park, 2000, Bioinformatics 16: 566-567).

In describing the variants of the present invention, the nomenclaturedescribed below is adapted for ease of reference. The accepted IUPACsingle letter or three letter amino acid abbreviation is employed.

Substitutions. For an amino acid substitution, the followingnomenclature is used: Original amino acid, position, substituted aminoacid. Accordingly, the substitution of threonine at position 226 withalanine is designated as “Thr226Ala” or “T226A”. Multiple mutations areseparated by addition marks (“+”), e.g. “Gly205Arg+Ser411Phe” or“G205R+S411F”, representing substitutions at positions 205 and 411 ofglycine (G) with arginine (R) and serine (S) with phenylalanine (F),respectively.

Deletions. For an amino acid deletion, the following nomenclature isused: Original amino acid, position, *. Accordingly, the deletion ofglycine at position 195 is designated as “Glyl95*” or “G195*”. Multipledeletions are separated by addition marks (“+”), e.g. “Glyl95*+Ser411*”or “G195*+S411*”.

Insertions. For an amino acid insertion, the following nomenclature isused: Original amino acid, position, original amino acid, inserted aminoacid. Accordingly, the insertion of lysine after glycine at position 195is designated “Glyl95GlyLys” or “G195GK”. An insertion of multiple aminoacids is designated [Original amino acid, position, original amino acid,inserted amino acid #1, inserted amino acid #2; etc.]. For example, theinsertion of lysine and alanine after glycine at position 195 isindicated as “Glyl95GlyLysAla” or “G195GKA”. An indication of aninsertion ata particular position is understood as being an insertionafter the original amino acid residue. For example, an “insertion atposition 195” is understood to be an insertion after the originalresidue in position 195.

In such cases the inserted amino acid residue(s) are numbered by theaddition of lower case letters to the position number of the amino acidresidue preceding the inserted amino acid residue(s). In the aboveexample, the sequence would thus be:

Parent: Variant: 195 195 195a 195b G G-K-A

Multiple alterations. Variants comprising multiple alterations areseparated by addition marks (“+”), e.g. “Arg170Tyr+Glyl95Glu” or“R170Y+G195E” representing a substitution of arginine and glycine atpositions 170 and 195 with tyrosine and glutamic acid, respectively.

Different alterations. Where different alterations can be introduced ata position, the different alterations are separated by a comma, e.g.“Arg170Tyr,Glu” represents a substitution of arginine at position 170with tyrosine or glutamic acid. Thus, “Tyr167Gly,Ala+Arg170Gly,Ala”designates the following variants: “Tyr167Gly+Arg170Gly”,“Tyr167Gly+Arg170Ala”, “Tyr167Ala+Arg170Gly”, and “Tyr167Ala+Arg170Ala”.Alternatively, different alterations or may be indicated using brackets,e.g. Arg170[Tyr, Gly] or in one-letter code R170[Y,G].

DETAILED DESCRIPTION OF THE INVENTION

The known xanthan endoglucanase having SEQ ID NO:2 and the xanthan lyasehaving SEQ ID NO:6 are both large enzymes (>1000 residues). It istherefore extremely laborious and expensive to target its properties forimprovement of, e.g. stability in a detergent composition, e.g. in thepresence of a chelator. In some aspects, the present invention narrowsdown the number of residues to target when trying to stabilizeendoglucanase molecules using protein engineering to a region selectedfrom the group consisting of: region 1 corresponding to amino acids 95to 105 of SEQ ID NO:2, region 2 corresponding to amino acids 115 to 138of SEQ ID NO:2, region 3 corresponding to amino acids 210 to 251 of SEQID NO:2, region 4 corresponding to amino acids 267 to 301 of SEQ IDNO:2, region 5 corresponding to amino acids 339 to 361 of SEQ ID NO:2,region 6 corresponding to amino acids 547 to 595 of SEQ ID NO:2, region7 corresponding to amino acids 612 to 660 of SEQ ID NO:2, region 8corresponding to amino acids 806 to 828 of SEQ ID NO:2, and region 9corresponding to amino acids 839 to 1042 of SEQ ID NO:2, and/or region10 corresponding to amino acids 1 to 94 of SEQ ID NO:2, region 11corresponding to amino acids 106 to 114 of SEQ ID NO:2, region 12corresponding to amino acids 139 to 209 of SEQ ID NO:2, region 13corresponding to amino acids 252 to 266 of SEQ ID NO:2, region 14corresponding to amino acids 302 to 338 of SEQ ID NO:2, region 15corresponding to amino acids 362 to 546 of SEQ ID NO:2, region 16corresponding to amino acids 596 to 611 of SEQ ID NO:2, region 17corresponding to amino acids 661 to 805 of SEQ ID NO:2, region 18corresponding to amino acids 829 to 838 of SEQ ID NO:2, and region 19corresponding to amino acids 1043 to 1055 of SEQ ID NO:2.

In some aspects, the present invention narrows down the number ofresidues to target when trying to stabilize xanthan lyase moleculesusing protein engineering to a region selected from the group consistingof: region 1 corresponding to amino acids 154 to 176 of SEQ ID NO:6,region 2 corresponding to amino acids 614 to 658 of SEQ ID NO:6, region3 corresponding to amino acids 731 to 803 of SEQ ID NO:6, region 4corresponding to amino acids 807 to 846 of SEQ ID NO:6, region 5corresponding to amino acids 872 to 885 of SEQ ID NO:6, and region 6corresponding to amino acids 903 to 1004 of SEQ ID NO:6, and/or region 7corresponding to amino acids 1 to 153 of SEQ ID NO:6, region 8corresponding to amino acids 177 to 613 of SEQ ID NO:6, region 9corresponding to amino acids 659 to 730 of SEQ ID NO:6, region 10corresponding to amino acids 804 to 806 of SEQ ID NO:6, region 11corresponding to amino acids 847 to 871 of SEQ ID NO:6, region 12corresponding to amino acids 886 to 902 of SEQ ID NO:6, and region 13corresponding to amino acids 1005 to 1037 of SEQ ID NO:6.

In one embodiment, the present invention dramatically narrows down thenumber of residues to target when trying to stabilizeendoglucanase/xanthan lyase molecules using protein engineering.

In another embodiment, the present invention provides detergentcompositions comprising variants of an endoglucanase and of a xanthanlyase, as described herein, both of which have significantly improvedstability as compared to the parent enzyme, such as the wild-typeendoglucanase/xanthan lyase. Such improved stability may be measured asimproved half-life of the variant compared to the parentendoglucanase/xanthan lyase, such as a wild-type endoglucanase/xanthanlyase. Furthermore, the stability of the variant is also proven to beimproved in the presence of a protease, which normally would cleaveproteins. The present invention discloses variants that have beenmodified so that they have an improved stability towards proteasecleavage.

Variants

In one embodiment, chelator-induced instability regions in the proteinsequence of the known xanthan endoglucanase having SEQ ID NO:2 that areaffected when the molecule is incubated in a buffer with EDTA, are thefollowing: region 1 corresponding to amino acids 95 to 105 of SEQ IDNO:2, region 2 corresponding to amino acids 115 to 138 of SEQ ID NO:2,region 3 corresponding to amino acids 210 to 251 of SEQ ID NO:2, region4 corresponding to amino acids 267 to 301 of SEQ ID NO:2, region 5corresponding to amino acids 339 to 361 of SEQ ID NO:2, region 6corresponding to amino acids 547 to 595 of SEQ ID NO:2, region 7corresponding to amino acids 612 to 660 of SEQ ID NO:2, region 8corresponding to amino acids 806 to 828 of SEQ ID NO:2, and region 9corresponding to amino acids 839 to 1042 of SEQ ID NO:2. This embodimentrelates to an important guidance on where to mutate an endoglucanase inorder to stabilize its molecule in a detergent, e.g. detergentcomposition comprising a chelator, e.g. EDTA or citrate.

Accordingly, in one embodiment the present invention relates to adetergent composition comprising an endoglucanase variant, comprising analteration (e.g. a substitution, deletion or insertion, preferably asubstitution) at one or more positions in a region selected from thegroup consisting of: region 1 corresponding to amino acids 95 to 105 ofSEQ ID NO:2, region 2 corresponding to amino acids 115 to 138 of SEQ IDNO:2, region 3 corresponding to amino acids 210 to 251 of SEQ ID NO:2,region 4 corresponding to amino acids 267 to 301 of SEQ ID NO:2, region5 corresponding to amino acids 339 to 361 of SEQ ID NO:2, region 6corresponding to amino acids 547 to 595 of SEQ ID NO:2, region 7corresponding to amino acids 612 to 660 of SEQ ID NO:2, region 8corresponding to amino acids 806 to 828 of SEQ ID NO:2, and region 9corresponding to amino acids 839 to 1042 of SEQ ID NO:2, wherein saidvariant has at least 60% and less than 100% sequence identity to SEQ IDNO:2; preferably said endoglucanase variant has activity on xanthan gumpretreated with xanthan lyase, further preferably said activity is axanthan gum degrading activity.

In one embodiment, regions in the protein sequence of the known xanthanendoglucanase having SEQ ID NO:2 that are affected when the molecule isincubated in a detergent, are the following: region 10 corresponding toamino acids 1 to 94 of SEQ ID NO:2, region 11 corresponding to aminoacids 106 to 114 of SEQ ID NO:2, region 12 corresponding to amino acids139 to 209 of SEQ ID NO:2, region 13 corresponding to amino acids 252 to266 of SEQ ID NO:2, region 14 corresponding to amino acids 302 to 338 ofSEQ ID NO:2, region 15 corresponding to amino acids 362 to 546 of SEQ IDNO:2, region 16 corresponding to amino acids 596 to 611 of SEQ ID NO:2,region 17 corresponding to amino acids 661 to 805 of SEQ ID NO:2, region18 corresponding to amino acids 829 to 838 of SEQ ID NO:2, and region 19corresponding to amino acids 1043 to 1055 of SEQ ID NO:2. Thisembodiment relates to an important guidance on where to mutate anendoglucanase in order to stabilize its molecule in a detergent.

In one embodiment the present invention relates to a detergentcomposition comprising an endoglucanase variant, comprising comprisingan alteration (e.g. a substitution, deletion or insertion, preferably asubstitution) at one or more positions in a region selected from thegroup consisting of: region 10 corresponding to amino acids 1 to 94 ofSEQ ID NO:2, region 11 corresponding to amino acids 106 to 114 of SEQ IDNO:2, region 12 corresponding to amino acids 139 to 209 of SEQ ID NO:2,region 13 corresponding to amino acids 252 to 266 of SEQ ID NO:2, region14 corresponding to amino acids 302 to 338 of SEQ ID NO:2, region 15corresponding to amino acids 362 to 546 of SEQ ID NO:2, region 16corresponding to amino acids 596 to 611 of SEQ ID NO:2, region 17corresponding to amino acids 661 to 805 of SEQ ID NO:2, region 18corresponding to amino acids 829 to 838 of SEQ ID NO:2, and region 19corresponding to amino acids 1043 to 1055 of SEQ ID NO:2; preferablysaid endoglucanase variant has activity on xanthan gum pretreated withxanthan lyase, further preferably said activity is a xanthan gumdegrading activity.

In one embodiment the present invention relates to a detergentcomposition comprising an endoglucanase variant, comprising analteration (e.g. a substitution, deletion or insertion, preferably asubstitution) at one or more positions in a region selected from thegroup consisting of:

i) region 1 corresponding to amino acids 95 to 105 of SEQ ID NO:2, e.g.said alteration at one or more positions selected from the groupconsisting of positions: 95, 96, 97, 98, 99, 100, 101, 102, 103, 104,and 105, wherein said positions correspond to amino acid positions ofSEQ ID NO:2 (e.g. using the numbering of SEQ ID NO:2),

ii) region 2 corresponding to amino acids 115 to 138 of SEQ ID NO:2,e.g. said alteration at one or more positions selected from the groupconsisting of positions: 115, 116, 117, 118, 119, 120, 121, 122, 123,124, 125, 126, 127, 128, 129, 130, 131, 132, 133, 134, 135, 136, 137,and 138, wherein said positions correspond to amino acid positions ofSEQ ID NO:2 (e.g. using the numbering of SEQ ID NO:2),

iii) region 3 corresponding to amino acids 210 to 251 of SEQ ID NO:2,e.g. said alteration at one or more positions selected from the groupconsisting of positions: 210, 211, 212, 213, 214, 215, 216, 217, 218,219, 220, 221, 222, 223, 224, 225, 226, 227, 228, 229, 230, 231, 232,233, 234, 235, 236, 237, 238, 239, 240, 241, 242, 243, 244, 245, 246,247, 248, 249, 250, and 251, wherein said positions correspond to aminoacid positions of SEQ ID NO:2 (e.g. using the numbering of SEQ ID NO:2),

iv) region 4 corresponding to amino acids 267 to 301 of SEQ ID NO:2,e.g. said alteration at one or more positions selected from the groupconsisting of positions: 267, 268, 269, 270, 271, 272, 273, 274, 275,276, 277, 278, 279, 280, 281, 282, 283, 284, 285, 286, 287, 288, 289,290, 291, 292, 293, 294, 295, 296, 297, 298, 299, 300, and 301, whereinsaid positions correspond to amino acid positions of SEQ ID NO:2 (e.g.using the numbering of SEQ ID NO:2),

v) region 5 corresponding to amino acids 339 to 361 of SEQ ID NO:2, e.g.said alteration at one or more positions selected from the groupconsisting of positions: 339, 340, 341, 342, 343, 344, 345, 346, 347,348, 349, 350, 351, 352, 353, 354, 355, 356, 357, 358, 359, 360, 361,wherein said positions correspond to amino acid positions of SEQ ID NO:2(e.g. using the numbering of SEQ ID NO:2),

vi) region 6 corresponding to amino acids 547 to 595 of SEQ ID NO:2,e.g. said alteration at one or more positions selected from the groupconsisting of positions: 547, 548, 549, 550, 551, 552, 553, 554, 555,556, 557, 558, 559, 560, 561, 562, 563, 564, 565, 566, 567, 568, 569,570, 571, 572, 573, 574, 575, 576, 577, 578, 579, 580, 581, 582, 583,584, 585, 586, 587, 588, 589, 590, 591, 592, 593, 594, and 595, whereinsaid positions correspond to amino acid positions of SEQ ID NO:2 (e.g.using the numbering of SEQ ID NO:2),

vii) region 7 corresponding to amino acids 612 to 660 of SEQ ID NO:2,e.g. said alteration at one or more positions selected from the groupconsisting of positions: 612, 613, 614, 615, 616, 617, 618, 619, 620,621, 622, 623, 624, 625, 626, 627, 628, 629, 630, 631, 632, 633, 634,635, 636, 637, 638, 639, 640, 641, 642, 643, 644, 645, 646, 647, 648,649, 650, 651, 652, 653, 654, 655, 656, 657, 658, 659, and 660, whereinsaid positions correspond to amino acid positions of SEQ ID NO:2 (e.g.using the numbering of SEQ ID NO:2),

viii) region 8 corresponding to amino acids 806 to 828 of SEQ ID NO:2,e.g. said alteration at one or more positions selected from the groupconsisting of positions: 806, 807, 808, 809, 810, 811, 812, 813, 814,815, 816, 817, 818, 819, 820, 821, 822, 823, 824, 825, 826, 827, and828, wherein said positions correspond to amino acid positions of SEQ IDNO:2 (e.g. using the numbering of SEQ ID NO:2), and

ix) region 9 corresponding to amino acids 839 to 1042 of SEQ ID NO:2,e.g. said alteration at one or more positions selected from the groupconsisting of positions: 839, 840, 841, 842, 843, 844, 845, 846, 847,848, 849, 850, 851, 852, 853, 854, 855, 856, 857, 858, 859, 860, 861,862, 863, 864, 865, 866, 867, 868, 869, 870, 871, 872, 873, 874, 875,876, 877, 878, 879, 880, 881, 882, 883, 884, 885, 886, 887, 888, 889,890, 891, 892, 893, 894, 895, 896, 897, 898, 899, 900, 901, 902, 903,904, 905, 906, 907, 908, 909, 910, 911, 912, 913, 914, 915, 916, 917,918, 919, 920, 921, 922, 923, 924, 925, 926, 927, 928, 929, 930, 931,932, 933, 934, 935, 936, 937, 938, 939, 940, 941, 942, 943, 944, 945,946, 947, 948, 949, 950, 951, 952, 953, 954, 955, 956, 957, 958, 959,960, 961, 962, 963, 964, 965, 966, 967, 968, 969, 970, 971, 972, 973,974, 975, 976, 977, 978, 979, 980, 981, 982, 983, 984, 985, 986, 987,988, 989, 990, 991, 992, 993, 994, 995, 996, 997, 998, 999, 1000, 1001,1002, 1003, 1004, 1005, 1006, 1007, 1008, 1009, 1010, 1011, 1012, 1013,1014, 1015, 1016, 1017, 1018, 1019, 1020, 1021, 1022, 1023, 1024, 1025,1026, 1027, 1028, 1029, 1030, 1031, 1032, 1033, 1034, 1035, 1036, 1037,1038, 1039, 1040, 1041, and 1042, wherein said positions correspond toamino acid positions of SEQ ID NO:2 (e.g. using the numbering of SEQ IDNO:2).

In one embodiment, the present invention relates to a detergentcomposition comprising an endoglucanase variant, comprising analteration (e.g. a substitution, deletion or insertion, preferably asubstitution) at one or more positions in a region selected from thegroup consisting of:

i) region 10 corresponding to amino acids 1 to 94 of SEQ ID NO:2, e.g.said alteration at one or more positions selected from the groupconsisting of positions: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14,15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32,33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50,51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68,69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86,87, 88, 89, 90, 91, 92, 93, 94, wherein said positions correspond toamino acid positions of SEQ ID NO:2 (e.g. using the numbering of SEQ IDNO:2),

ii) region 11 corresponding to amino acids 106 to 114 of SEQ ID NO:2,e.g. said alteration at one or more positions selected from the groupconsisting of positions: 107, 108, 109, 110, 111, 112, 113, 114, whereinsaid positions correspond to amino acid positions of SEQ ID NO:2 (e.g.using the numbering of SEQ ID NO:2),

iii) region 12 corresponding to amino acids 139 to 209of SEQ ID NO:2,e.g. said alteration at one or more positions selected from the groupconsisting of positions: 139, 140, 141, 142, 143, 144, 145, 146, 147,148, 149, 150, 151, 152, 153, 154, 155, 156, 157, 158, 159, 160, 161,162, 163, 164, 165, 166, 167, 168, 169, 170, 171, 172, 173, 174, 175,176, 177, 178, 179, 180, 181, 182, 183, 184, 185, 186, 187, 188, 189,190, 191, 192, 193, 194, 195, 196, 197, 198, 199, 200, 201, 202, 203,204, 205, 206, 207, 208, 209, wherein said positions correspond to aminoacid positions of SEQ ID NO:2 (e.g. using the numbering of SEQ ID NO:2),

iv) region 13 corresponding to amino acids 252 to 266 of SEQ ID NO:2,e.g. said alteration at one or more positions selected from the groupconsisting of positions: 252, 253, 254, 255, 256, 257, 258, 259, 260,261, 262, 263, 264, 265, 266, wherein said positions correspond to aminoacid positions of SEQ ID NO:2 (e.g. using the numbering of SEQ ID NO:2),

v) region 14 corresponding to amino acids 302 to 338 of SEQ ID NO:2,e.g. said alteration at one or more positions selected from the groupconsisting of positions: 302, 303, 304, 305, 306, 307, 308, 309, 310,311, 312, 313, 314, 315, 316, 317, 318, 319, 320, 321, 322, 323, 324,325, 326, 327, 328, 329, 330, 331, 332, 333, 334, 335, 336, 337, 338,wherein said positions correspond to amino acid positions of SEQ ID NO:2(e.g. using the numbering of SEQ ID NO:2),

vi) region 15 corresponding to amino acids 362 to 546 of SEQ ID NO:2,e.g. said alteration at one or more positions selected from the groupconsisting of positions: 362, 363, 364, 365, 366, 367, 368, 369, 370,371, 372, 373, 374, 375, 376, 377, 378, 379, 380, 381, 382, 383, 384,385, 386, 387, 388, 389, 390, 391, 392, 393, 394, 395, 396, 397, 398,399, 400, 401, 402, 403, 404, 405, 406, 407, 408, 409, 410, 411, 412,413, 414, 415, 416, 417, 418, 419, 420, 421, 422, 423, 424, 425, 426,427, 428, 429, 430, 431, 432, 433, 434, 435, 436, 437, 438, 439, 440,441, 442, 443, 444, 445, 446, 447, 448, 449, 450, 451, 452, 453, 454,455, 456, 457, 458, 459, 460, 461, 462, 463, 464, 465, 466, 467, 468,469, 470, 471, 472, 473, 474, 475, 476, 477, 478, 479, 480, 481, 482,483, 484, 485, 486, 487, 488, 489, 490, 491, 492, 493, 494, 495, 496,497, 498, 499, 500, 501, 502, 503, 504, 505, 506, 507, 508, 509, 510,511, 512, 513, 514, 515, 516, 517, 518, 519, 520, 521, 522, 523, 524,525, 526, 527, 528, 529, 530, 531, 532, 533, 534, 535, 536, 537, 538,539, 540, 541, 542, 543, 544, 545, 546, wherein said positionscorrespond to amino acid positions of SEQ ID NO:2 (e.g. using thenumbering of SEQ ID NO:2),

vii) region 16 corresponding to amino acids 596 to 611 of SEQ ID NO:2,e.g. said alteration at one or more positions selected from the groupconsisting of positions: 596, 597, 598, 599, 600, 601, 602, 603, 604,605, 606, 607, 608, 609, 610, 611, wherein said positions correspond toamino acid positions of SEQ ID NO:2 (e.g. using the numbering of SEQ IDNO:2),

viii) region 17 corresponding to amino acids 661 to 805 of SEQ ID NO:2,e.g. said alteration at one or more positions selected from the groupconsisting of positions: 661, 662, 663, 664, 665, 666, 667, 668, 669,670, 671, 672, 673, 674, 675, 676, 677, 678, 679, 680, 681, 682, 683,684, 685, 686, 687, 688, 689, 690, 691, 692, 693, 694, 695, 696, 697,698, 699, 700, 701, 702, 703, 704, 705, 706, 707, 708, 709, 710, 711,712, 713, 714, 715, 716, 717, 718, 719, 720, 721, 722, 723, 724, 725,726, 727, 728, 729, 730, 731, 732, 733, 734, 735, 736, 737, 738, 739,740, 741, 742, 743, 744, 745, 746, 747, 748, 749, 750, 751, 752, 753,754, 755, 756, 757, 758, 759, 760, 761, 762, 763, 764, 765, 766, 767,768, 769, 770, 771, 772, 773, 774, 775, 776, 777, 778, 779, 780, , 781,782, 783, 784, 785, 786, 787, 788, 789, 790, 791, 792, 793, 794, 795,796, 797, 798, 799, 800, 801, 802, 803, 804, 805, wherein said positionscorrespond to amino acid positions of SEQ ID NO:2 (e.g. using thenumbering of SEQ ID NO:2),

ix) region 18 corresponding to amino acids 829 to 838 of SEQ ID NO:2,e.g. said alteration at one or more positions selected from the groupconsisting of positions: 829, 830, 831, 832, 833, 834, 835, 836, 837,838, wherein said positions correspond to amino acid positions of SEQ IDNO:2 (e.g. using the numbering of SEQ ID NO:2) x) region 19corresponding to amino acids 1043 to 1055 of SEQ ID NO:2, e.g. saidalteration at one or more positions selected from the group consistingof positions 1043, 1044, 1045, 1046, 1047, 1048, 1049, 1050, 1051, 1052,1053, 1054, 1055, wherein said positions correspond to amino acidpositions of SEQ ID NO:2 (e.g. using the numbering of SEQ ID NO:2).

In one embodiment the present invention relates to a detergentcomposition comprising an endoglucanase variant, comprising analteration (e.g. a substitution, deletion or insertion, preferably asubstitution) at one or more positions in one or more regions ormultiple alterations (such as 2, 3, 4, 5, 6, 7, 8, 9 or 10) in oneregion or multiple alterations (e.g. 2, 3, 4, 5, 6, 7, 8, 9 or 10) inmultiple regions (e.g. 2, 3, 4, 5, 6, 7, 8, or 9) selected from thegroup consisting of: region 1 corresponding to amino acids 95 to 105 ofSEQ ID NO:2, region 2 corresponding to amino acids 115 to 138 of SEQ IDNO:2, region 3 corresponding to amino acids 210 to 251 of SEQ ID NO:2,region 4 corresponding to amino acids 267 to 301 of SEQ ID NO:2, region5 corresponding to amino acids 339 to 361 of SEQ ID NO:2, region 6corresponding to amino acids 547 to 595 of SEQ ID NO:2, region 7corresponding to amino acids 612 to 660 of SEQ ID NO:2, region 8corresponding to amino acids 806 to 828 of SEQ ID NO:2, and region 9corresponding to amino acids 839 to 1042 of SEQ ID NO:2, wherein saidvariant has at least 60% and less than 100% sequence identity to SEQ IDNO:2; preferably said endoglucanase variant has activity on xanthan gumpretreated with xanthan lyase, further preferably said activity is axanthan gum degrading activity.

In one embodiment the present invention relates to a detergentcomposition comprising an endoglucanase variant, comprising analteration (e.g. a substitution, deletion or insertion, preferably asubstitution) at one or more positions in one or more regions selectedfrom the group consisting of: region 10 corresponding to amino acids 1to 94 of SEQ ID NO:2, region 11 corresponding to amino acids 106 to 114of SEQ ID NO:2, region 12 corresponding to amino acids 139 to 209 of SEQID NO:2, region 13 corresponding to amino acids 252 to 266 of SEQ IDNO:2, region 14 corresponding to amino acids 302 to 338 of SEQ ID NO:2,region 15 corresponding to amino acids 362 to 546 of SEQ ID NO:2, region16 corresponding to amino acids 596 to 611 of SEQ ID NO:2, region 17corresponding to amino acids 661 to 805 of SEQ ID NO:2, region 18corresponding to amino acids 829 to 838 of SEQ ID NO:2, and region 19corresponding to amino acids 1043 to 1055 of SEQ ID NO:2, wherein saidvariant has at least 60% and less than 100% sequence identity to SEQ IDNO:2; preferably said endoglucanase variant has activity on xanthan gumpretreated with xanthan lyase, further preferably said activity is axanthan gum degrading activity.

In one embodiment the present invention relates to a detergentcomposition comprising an endoglucanase variant as described hereinhaving multiple alterations (such as 2, 3, 4, 5, 6, 7, 8, 9 or 10) inone region (e.g. of SEQ ID NO:2 or another parent endoglucanase)selected from the group consisting of: region 10 corresponding to aminoacids 1 to 94 of SEQ ID NO:2, region 11 corresponding to amino acids 106to 114 of SEQ ID NO:2, region 12 corresponding to amino acids 139 to 209of SEQ ID NO:2, region 13 corresponding to amino acids 252 to 266 of SEQID NO:2, region 14 corresponding to amino acids 302 to 338 of SEQ IDNO:2, region 15 corresponding to amino acids 362 to 546 of SEQ ID NO:2,region 16 corresponding to amino acids 596 to 611 of SEQ ID NO:2, region17 corresponding to amino acids 661 to 805 of SEQ ID NO:2, region 18corresponding to amino acids 829 to 838 of SEQ ID NO:2, and region 19corresponding to amino acids 1043 to 1055 of SEQ ID NO:2, wherein saidvariant has at least 60% and less than 100% sequence identity to SEQ IDNO:2, preferably said endoglucanase variant has activity on xanthan gumpretreated with xanthan lyase, further preferably said activity is axanthan gum degrading activity.

In one embodiment the present invention relates to a detergentcomposition comprising an endoglucanase variant as described hereinhaving multiple alterations (e.g. 2, 3, 4, 5, 6, 7, 8, 9 or 10) inmultiple regions (e.g. 2, 3, 4, 5, 6, 7, 8, 9 or 10) (e.g. of SEQ IDNO:2 or another parent endoglucanase) selected from the group consistingof: region 10 corresponding to amino acids 1 to 94 of SEQ ID NO:2,region 11 corresponding to amino acids 106 to 114 of SEQ ID NO:2, region12 corresponding to amino acids 139 to 209 of SEQ ID NO:2, region 13corresponding to amino acids 252 to 266 of SEQ ID NO:2, region 14corresponding to amino acids 302 to 338 of SEQ ID NO:2, region 15corresponding to amino acids 362 to 546 of SEQ ID NO:2, region 16corresponding to amino acids 596 to 611 of SEQ ID NO:2, region 17corresponding to amino acids 661 to 805 of SEQ ID NO:2, region 18corresponding to amino acids 829 to 838 of SEQ ID NO:2, and region 19corresponding to amino acids 1043 to 1055 of SEQ ID NO:2, preferablysaid endoglucanase variant has activity on xanthan gum pretreated withxanthan lyase, further preferably said activity is a xanthan gumdegrading activity. In one embodiment the present invention relates to adetergent composition comprising a parent endoglucanase of the invention(e.g. SEQ ID NO:2) having an alteration (e.g. a substitution, deletionor insertion, preferably a substitution) at one or more positions in aregion selected from the group consisting of regions 1-9, wherein saidregion is a chelator-induced instability region, preferably saidchelator-induced instability region has one or more of the followingfeatures: in the presence of a chelator it is less conformationallystable than one or more or all of its adjacent regions; and/or in thepresence of a chelator it is more exposed to said chelator than one ormore or all of its adjacent regions; and/or in the presence of achelator it is more accessible to said chelator than one or more or allof its adjacent regions; and/or in the presence of a chelator it is moreconformationally dynamic than one or more or all of its adjacentregions; and/or in the presence of a chelator it is more receptive todeuterium incorporation than one or more or all of its adjacent regions;further preferably said adjacent region is selected from the groupconsisting of: region 10 corresponding to amino acids 1 to 94 of SEQ IDNO:2, region 11 corresponding to amino acids 106 to 114 of SEQ ID NO:2,region 12 corresponding to amino acids 139 to 209 of SEQ ID NO:2, region13 corresponding to amino acids 252 to 266 of SEQ ID NO:2, region 14corresponding to amino acids 302 to 338 of SEQ ID NO:2, region 15corresponding to amino acids 362 to 546 of SEQ ID NO:2, region 16corresponding to amino acids 596 to 611 of SEQ ID NO:2, region 17corresponding to amino acids 661 to 805 of SEQ ID NO:2, region 18corresponding to amino acids 829 to 838 of SEQ ID NO:2, and region 19corresponding to amino acids 1043 to 1055 of SEQ ID NO:2; further mostpreferably said chelator is EDTA or citrate.

In one embodiment the adjacent regions referred to herein can be one ormore or all of the following: region 10 corresponding to amino acids 1to 94 of SEQ ID NO:2, region 11 corresponding to amino acids 106 to 114of SEQ ID NO:2, region 12 corresponding to amino acids 139 to 209 of SEQID NO:2, region 13 corresponding to amino acids 252 to 266 of SEQ IDNO:2, region 14 corresponding to amino acids 302 to 338 of SEQ ID NO:2,region 15 corresponding to amino acids 362 to 546 of SEQ ID NO:2, region16 corresponding to amino acids 596 to 611 of SEQ ID NO:2, region 17corresponding to amino acids 661 to 805 of SEQ ID NO:2, region 18corresponding to amino acids 829 to 838 of SEQ ID NO:2, and region 19corresponding to amino acids 1043 to 1055 of SEQ ID NO:2.

In one embodiment the present invention relates to a detergentcomposition comprising an endoglucanase variant of the invention havingan alteration (e.g. a substitution, deletion or insertion, preferably asubstitution) at one or more positions in a region selected from thegroup consisting of regions 1-9 (e.g. of SEQ ID NO:2 or another parentendoglucanase), wherein in an aqueous solution comprising a detergentcomponent said region (e.g. of SEQ ID NO:2 or another parentendoglucanase) is relatively more accessible to said detergent componentthan one or more or all of its adjacent regions.

In one embodiment the present invention relates to a detergentcomposition comprising an endoglucanase variant of the invention havingan alteration (e.g. a substitution, deletion or insertion, preferably asubstitution) at one or more positions in a region selected from thegroup consisting of regions 1-9 (e.g. of SEQ ID NO:2 or another parentendoglucanase), wherein in an aqueous solution comprising a detergentcomponent said region (e.g. of SEQ ID NO:2 or another parentendoglucanase) is relatively more exposed to said detergent componentthan one or more or all of its adjacent regions.

In one embodiment the present invention relates to a detergentcomposition comprising an endoglucanase variant of the invention havingan alteration (e.g. a substitution, deletion or insertion, preferably asubstitution) at one or more positions in a region selected from thegroup consisting of regions 1-9 (e.g. of SEQ ID NO:2 or another parentendoglucanase), wherein in an aqueous solution comprising a detergentcomponent said region (e.g. of SEQ ID NO:2 or another parentendoglucanase) is relatively more accessible to said detergent componentthan one or more or all of its adjacent regions.

In one embodiment the present invention relates to a detergentcomposition comprising an endoglucanase variant of the invention havingan alteration (e.g. a substitution, deletion or insertion, preferably asubstitution) at one or more positions in a region selected from thegroup consisting of regions 1-9 (e.g. of SEQ ID NO:2 or another parentendoglucanase), wherein in an aqueous solution comprising a detergentcomponent said region (e.g. of SEQ ID NO:2 or another parentendoglucanase) is relatively more conformationally dynamic than one ormore or all of its adjacent regions.

In one embodiment the present invention relates to a detergentcomposition comprising an endoglucanase variant of the invention havingan alteration (e.g. a substitution, deletion or insertion, preferably asubstitution) at one or more positions in a region selected from thegroup consisting of regions 1-9 (e.g. of SEQ ID NO:2 or another parentendoglucanase), wherein in an aqueous solution comprising a detergentcomponent said region (e.g. of SEQ ID NO:2 or another parentendoglucanase) is relatively more receptive to deuterium incorporationthan one or more or all of its adjacent regions.

In one embodiment the present invention relates to a detergentcomposition comprising an endoglucanase variant of the invention,further comprising an alteration (e.g. a substitution, deletion orinsertion, preferably a substitution) at one or more positions in one ormore regions selected from the group consisting of: region 1corresponding to amino acids 95 to 105 of SEQ ID NO:2, region 2corresponding to amino acids 115 to 138 of SEQ ID NO:2, region 3corresponding to amino acids 210 to 251 of SEQ ID NO:2, region 4corresponding to amino acids 267 to 301 of SEQ ID NO:2, region 5corresponding to amino acids 339 to 361 of SEQ ID NO:2, region 6corresponding to amino acids 547 to 595 of SEQ ID NO:2, region 7corresponding to amino acids 612 to 660 of SEQ ID NO:2, region 8corresponding to amino acids 806 to 828 of SEQ ID NO:2, and region 9corresponding to amino acids 839 to 1042 of SEQ ID NO:2, wherein saidvariant has at least 60% and less than 100% sequence identity to SEQ IDNO:2, preferably said endoglucanase variant has activity on xanthan gumpretreated with xanthan lyase, further preferably said activity is axanthan gum degrading activity.

In one embodiment the present invention relates to a detergentcomposition comprising an endoglucanase variant of the invention havingmultiple alterations (such as 2, 3, 4, 5, 6, 7, 8, 9 or 10) in oneregion (e.g. of SEQ ID NO:2 or another parent endoglucanase) selectedfrom the group consisting of: region 1 corresponding to amino acids 95to 105 of SEQ ID NO:2, region 2 corresponding to amino acids 115 to 138of SEQ ID NO:2, region 3 corresponding to amino acids 210 to 251 of SEQID NO:2, region 4 corresponding to amino acids 267 to 301 of SEQ IDNO:2, region 5 corresponding to amino acids 339 to 361 of SEQ ID NO:2,region 6 corresponding to amino acids 547 to 595 of SEQ ID NO:2, region7 corresponding to amino acids 612 to 660 of SEQ ID NO:2, region 8corresponding to amino acids 806 to 828 of SEQ ID NO:2, and region 9corresponding to amino acids 839 to 1042 of SEQ ID NO:2, wherein saidvariant has at least 60% and less than 100% sequence identity to SEQ IDNO:2, preferably said endoglucanase variant has activity on xanthan gumpretreated with xanthan lyase, further preferably said activity is axanthan gum degrading activity.

In one embodiment the present invention relates to a detergentcomposition comprising an endoglucanase variant of the invention havingmultiple alterations (e.g. 2, 3, 4, 5, 6, 7, 8, 9 or 10) in multipleregions (e.g. 2, 3, 4, 5, 6, 7, 8, or 9) (e.g. of SEQ ID NO:2 or anotherparent endoglucanase) selected from the group consisting of: region 1corresponding to amino acids 95 to 105 of SEQ ID NO:2, region 2corresponding to amino acids 115 to 138 of SEQ ID NO:2, region 3corresponding to amino acids 210 to 251 of SEQ ID NO:2, region 4corresponding to amino acids 267 to 301 of SEQ ID NO:2, region 5corresponding to amino acids 339 to 361 of SEQ ID NO:2, region 6corresponding to amino acids 547 to 595 of SEQ ID NO:2, region 7corresponding to amino acids 612 to 660 of SEQ ID NO:2, region 8corresponding to amino acids 806 to 828 of SEQ ID NO:2, and region 9corresponding to amino acids 839 to 1042 of SEQ ID NO:2, wherein saidvariant has at least 60% and less than 100% sequence identity to SEQ IDNO:2, preferably said endoglucanase variant has activity on xanthan gumpretreated with xanthan lyase, further preferably said activity is axanthan gum degrading activity.

In a further embodiment, the present invention relates to a detergentcomposition comprising an endoglucanase variant of the invention havingmultiple alterations (e.g. 2, 3, 4, 5, 6, 7, 8, 9 or 10) in multipleregions (e.g. 2, 3, 4, 5, 6, 7, 8, or 9) (e.g. of SEQ ID NO:2 or anotherparent endoglucanase) selected from the group consisting of: region 1corresponding to amino acids 95 to 105 of SEQ ID NO:2, region 2corresponding to amino acids 115 to 138 of SEQ ID NO:2, region 3corresponding to amino acids 210 to 251 of SEQ ID NO:2, region 4corresponding to amino acids 267 to 301 of SEQ ID NO:2, region 5corresponding to amino acids 339 to 361 of SEQ ID NO:2, region 6corresponding to amino acids 547 to 595 of SEQ ID NO:2, region 7corresponding to amino acids 612 to 660 of SEQ ID NO:2, region 8corresponding to amino acids 806 to 828 of SEQ ID NO:2, and region 9corresponding to amino acids 839 to 1042 of SEQ ID NO:2, and multiplealterations (e.g. 2, 3, 4, 5, 6, 7, 8, 9, or 10) in multiple adjacentregions (e.g. 2, 3, 4, 5, 6, 7, 8, 9, or 10) selected from the groupconsisting of: region 10 corresponding to amino acids 1 to 94 of SEQ IDNO:2, region 11 corresponding to amino acids 106 to 114 of SEQ ID NO:2,region 12 corresponding to amino acids 139 to 209 of SEQ ID NO:2, region13 corresponding to amino acids 252 to 266 of SEQ ID NO:2, region 14corresponding to amino acids 302 to 338 of SEQ ID NO:2, region 15corresponding to amino acids 362 to 546 of SEQ ID NO:2, region 16corresponding to amino acids 596 to 611 of SEQ ID NO:2, region 17corresponding to amino acids 661 to 805 of SEQ ID NO:2, region 18corresponding to amino acids 829 to 838 of SEQ ID NO:2, and region 19corresponding to amino acids 1043 to 1055 of SEQ ID NO:2; wherein saidvariant has at least 60% and less than 100% sequence identity to SEQ IDNO:2, preferably said endoglucanase variant has activity on xanthan gumpretreated with xanthan lyase, further preferably said activity is axanthan gum degrading activity.

In one embodiment, the present invention relates to a detergentcomposition comprising endoglucanase variants, comprising an alteration(e.g. a substitution, deletion or insertion, preferably a substitution)at one or more (e.g. several) positions of the mature parent polypeptide(e.g. SEQ ID NO:2), wherein each alteration is independently asubstitution, insertion or deletion, wherein the variant hasendoglucanase activity.

In an embodiment, the variant has sequence identity of at least 60%,e.g. at least 65%, 70%, 75%, 80%, 85%, 90%, 91%, 92%, 93%, 94%, 95%,96%, 97%, 98%, or 99%, but less than 100%, to the amino acid sequence ofthe parent endoglucanase.

In one embodiment, the variant has at least 60%, e.g. at least 65%, 70%,75%, 80%, 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, or 99%, butless than 100%, sequence identity to the mature polypeptide of SEQ IDNO:2.

In one embodiment the present invention relates to a detergentcomposition comprising an endoglucanase variant of the invention,wherein said variant has at least 61%, 62%, 63%, 64%, 65%, 66%, 67%,68%, 69%, 70%, 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%,82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%,96%, 97%, 98%, or 99% sequence identity to SEQ ID NO:2.

In another aspect, a variant comprises an alteration at one or more(e.g. several) positions corresponding to positions 4, 17, 18, 20, 51,53, 55, 56, 60, 63, 71, 79, 87, 92, 99, 120, 125, 126, 130, 137, 182,186, 189, 192, 213, 216, 221, 226, 228, 230, 231, 232, 233, 235, 240,243, 247, 249, 278, 279, 281, 283, 285, 289, 292, 294, 298, 302, 311,313, 333, 346, 353, 358, 386, 387, 388, 390, 403, 408, 410, 416, 441,448, 451, 471, 472, 476, 489, 507, 512, 515, 538, 555, 556, 557, 558,559, 560, 561, 562, 563, 564, 567, 568, 570, 575, 578, 579, 580, 581,583, 589, 590, 591, 592, 593, 595, 598, 599, 602, 603, 605, 607, 609,616, 627, 630, 631, 635, 636, 638, 639, 640, 641, 642, 643, 644, 651,676, 683, 688, 690, 694, 698, 699, 706, 711, 713, 1719, 720, 744, 749,754, 756, 760, , 781, 786, 797, 810, 811, 812, 815, 823, 824, 825, 827,828, 833, 834, 835, 837, 843, 848, 868, 869, 870, 871, 872, 873, 874,880, 881, 883, 884, 885, 887, 888, 890, 892, 894, 898, 905, 906, 912,920, 921, 924, 926, 927, 928, 932, 933, 934, 935, 937, 938, 939, 940,941, 942, 943, 946, 948, 950, 952, 953, 954, 956, 957, 960, 966, 971,972, 980, 989, 991, 994, 995, 998, 999, 1006, 1009, 1010, 1011, 1029,1030, 1031, 1032, 1035, 1037, 1038, 1040, 1041, 1042, 1044, 1045, 1048,wherein numbering is according to SEQ ID NO:2.

In another aspect, a variant comprises an alteration at two positionscorresponding to any of positions 4, 17, 18, 20, 51, 53, 55, 56, 60, 63,71, 79, 87, 92, 99, 120, 125, 126, 130, 137, 182, 186, 189, 192, 213,216, 221, 226, 228, 230, 231, 232, 233, 235, 240, 243, 247, 249, 278,279, 281, 283, 285, 289, 292, 294, 298, 302, 311, 313, 333, 346, 353,358, 386, 387, 388, 390, 403, 408, 410, 416, 441, 448, 451, 471, 472,476, 489, 507, 512, 515, 538, 555, 556, 557, 558, 559, 560, 561, 562,563, 564, 567, 568, 570, 575, 578, 579, 580, 581, 583, 589, 590, 591,592, 593, 595, 598, 599, 602, 603, 605, 607, 609, 616, 627, 630, 631,635, 636, 638, 639, 640, 641, 642, 643, 644, 651, 676, 683, 688, 690,694, 698, 699, 706, 711, 713, 1719, 720, 744, 749, 754, 756, 760, , 781,786, 797, 810, 811, 812, 815, 823, 824, 825, 827, 828, 833, 834, 835,837, 843, 848, 868, 869, 870, 871, 872, 873, 874, 880, 881, 883, 884,885, 887, 888, 890, 892, 894, 898, 905, 906, 912, 920, 921, 924, 926,927, 928, 932, 933, 934, 935, 937, 938, 939, 940, 941, 942, 943, 946,948, 950, 952, 953, 954, 956, 957, 960, 966, 971, 972, 980, 989, 991,994, 995, 998, 999, 1006, 1009, 1010, 1011, 1029, 1030, 1031, 1032,1035, 1037, 1038, 1040, 1041, 1042, 1044, 1045, 1048, wherein numberingis according to SEQ ID NO:2.

In another aspect, a variant comprises an alteration at three positionscorresponding to any of positions 4, 17, 18, 20, 51, 53, 55, 56, 60, 63,71, 79, 87, 92, 99, 120, 125, 126, 130, 137, 182, 186, 189, 192, 213,216, 221, 226, 228, 230, 231, 232, 233, 235, 240, 243, 247, 249, 278,279, 281, 283, 285, 289, 292, 294, 298, 302, 311, 313, 333, 346, 353,358, 386, 387, 388, 390, 403, 408, 410, 416, 441, 448, 451, 471, 472,476, 489, 507, 512, 515, 538, 555, 556, 557, 558, 559, 560, 561, 562,563, 564, 567, 568, 570, 575, 578, 579, 580, 581, 583, 589, 590, 591,592, 593, 595, 598, 599, 602, 603, 605, 607, 609, 616, 627, 630, 631,635, 636, 638, 639, 640, 641, 642, 643, 644, 651, 676, 683, 688, 690,694, 698, 699, 706, 711, 713, 1719, 720, 744, 749, 754, 756, 760, , 781,786, 797, 810, 811, 812, 815, 823, 824, 825, 827, 828, 833, 834, 835,837, 843, 848, 868, 869, 870, 871, 872, 873, 874, 880, 881, 883, 884,885, 887, 888, 890, 892, 894, 898, 905, 906, 912, 920, 921, 924, 926,927, 928, 932, 933, 934, 935, 937, 938, 939, 940, 941, 942, 943, 946,948, 950, 952, 953, 954, 956, 957, 960, 966, 971, 972, 980, 989, 991,994, 995, 998, 999, 1006, 1009, 1010, 1011, 1029, 1030, 1031, 1032,1035, 1037, 1038, 1040, 1041, 1042, 1044, 1045, 1048, wherein numberingis according to SEQ ID NO:2.

In another aspect, a variant comprises an alteration at each position(or at least four positions) corresponding to positions 4, 17, 18, 20,51, 53, 55, 56, 60, 63, 71, 79, 87, 92, 99, 120, 125, 126, 130, 137,182, 186, 189, 192, 213, 216, 221, 226, 228, 230, 231, 232, 233, 235,240, 243, 247, 249, 278, 279, 281, 283, 285, 289, 292, 294, 298, 302,311, 313, 333, 346, 353, 358, 386, 387, 388, 390, 403, 408, 410, 416,441, 448, 451, 471, 472, 476, 489, 507, 512, 515, 538, 555, 556, 557,558, 559, 560, 561, 562, 563, 564, 567, 568, 570, 575, 578, 579, 580,581, 583, 589, 590, 591, 592, 593, 595, 598, 599, 602, 603, 605, 607,609, 616, 627, 630, 631, 635, 636, 638, 639, 640, 641, 642, 643, 644,651, 676, 683, 688, 690, 694, 698, 699, 706, 711, 713, 1719, 720, 744,749, 754, 756, 760, , 781, 786, 797, 810, 811, 812, 815, 823, 824, 825,827, 828, 833, 834, 835, 837, 843, 848, 868, 869, 870, 871, 872, 873,874, 880, 881, 883, 884, 885, 887, 888, 890, 892, 894, 898, 905, 906,912, 920, 921, 924, 926, 927, 928, 932, 933, 934, 935, 937, 938, 939,940, 941, 942, 943, 946, 948, 950, 952, 953, 954, 956, 957, 960, 966,971, 972, 980, 989, 991, 994, 995, 998, 999, 1006, 1009, 1010, 1011,1029, 1030, 1031, 1032, 1035, 1037, 1038, 1040, 1041, 1042, 1044, 1045,1048, wherein numbering is according to SEQ ID NO:2.

In another aspect, a variant comprises an alteration at each position(or at least four positions) corresponding to positions 17, 20, 51, 53,55, 56, 60, 63, 79, 87, 192, 302, 387, 388, 390, 403, 408, 410, 416,448, 451, 471, 472, 507, 512, 515, 538, 598, 602, 605, 609, 676, 694,698, 699, 711, 754, 760, , 781, 786, 797, 834, and 835 of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 4. In another aspect, the aminoacid at a position corresponding to position 4 is substituted with Ala,Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro,Ser, Thr, Trp, Tyr, or Val. In another aspect, the variant comprises orconsists of the substitution V4T of the mature polypeptide of SEQ IDNO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 17. In another aspect, the aminoacid at a position corresponding to position 17 is substituted with Ala,Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro,Ser, Thr, Trp, Tyr, or Val. In another aspect, the variant comprises orconsists of the substitution S17A of the mature polypeptide of SEQ IDNO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 18. In another aspect, the aminoacid at a position corresponding to position 18 is substituted with Ala,Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro,Ser, Thr, Trp, Tyr, or Val. In another aspect, the variant comprises orconsists of the substitution N18G of the mature polypeptide of SEQ IDNO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 20. In another aspect, the aminoacid at a position corresponding to position 20 is substituted with Ala,Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro,Ser, Thr, Trp, Tyr, or Val. In another aspect, the variant comprises orconsists of the substitution F20P, F20N, F20G, or F20Y, preferably F20P,of the mature polypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 51. In another aspect, the aminoacid at a position corresponding to position 51 is substituted with Ala,Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro,Ser, Thr, Trp, Tyr, or Val. In another aspect, the variant comprises orconsists of the substitution K51Q or K51H, preferably K51Q, of themature polypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 53. In another aspect, the aminoacid at a position corresponding to position 53 is substituted with Ala,Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro,Ser, Thr, Trp, Tyr, or Val. In another aspect, the variant comprises orconsists of the substitution E53Y, E53P, or E53G, preferably E53Y, ofthe mature polypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 55. In another aspect, the aminoacid at a position corresponding to position 55 is substituted with Ala,Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro,Ser, Thr, Trp, Tyr, or Val. In another aspect, the variant comprises orconsists of the substitution Y55M or Y55D, preferably Y55M, of themature polypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 56. In another aspect, the aminoacid at a position corresponding to position 56 is substituted with Ala,Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro,Ser, Thr, Trp, Tyr, or Val. In another aspect, the variant comprises orconsists of the substitution V56M of the mature polypeptide of SEQ IDNO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 60. In another aspect, the aminoacid at a position corresponding to position 60 is substituted with Ala,Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro,Ser, Thr, Trp, Tyr, or Val. In another aspect, the variant comprises orconsists of the substitution Y6OF of the mature polypeptide of SEQ IDNO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 63. In another aspect, the aminoacid at a position corresponding to position 63 is substituted with Ala,Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro,Ser, Thr, Trp, Tyr, or Val. In another aspect, the variant comprises orconsists of the substitution S63F of the mature polypeptide of SEQ IDNO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 71. In another aspect, the aminoacid at a position corresponding to position 71 is substituted with Ala,Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro,Ser, Thr, Trp, Tyr, or Val. In another aspect, the variant comprises orconsists of the substitution A71E of the mature polypeptide of SEQ IDNO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 79. In another aspect, the aminoacid at a position corresponding to position 79 is substituted with Ala,Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro,Ser, Thr, Trp, Tyr, or Val. In another aspect, the variant comprises orconsists of the substitution S79W of the mature polypeptide of SEQ IDNO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 87. In another aspect, the aminoacid at a position corresponding to position 87 is substituted with Ala,Arg, Asn, Asp, Cys, Gin, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro,Ser, Thr, Trp, Tyr, or Val. In another aspect, the variant comprises orconsists of the substitution T87R of the mature polypeptide of SEQ IDNO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 92. In another aspect, the aminoacid at a position corresponding to position 92 is substituted with Ala,Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro,Ser, Thr, Trp, Tyr, or Val. In another aspect, the variant comprises orconsists of the substitution T92S of the mature polypeptide of SEQ IDNO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 120. In another aspect, the aminoacid at a position corresponding to position 120 is substituted withAla, Arg, Asn, Asp, Cys, Gin, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution A120P of the maturepolypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 129. In another aspect, the aminoacid at a position corresponding to position 129 is substituted withAla, Arg, Asn, Asp, Cys, Gin, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution N129D of the maturepolypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 137. In another aspect, the aminoacid at a position corresponding to position 137 is substituted withAla, Arg, Asn, Asp, Cys, Gin, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution F137L of the maturepolypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 182. In another aspect, the aminoacid at a position corresponding to position 182 is substituted withAla, Arg, Asn, Asp, Cys, Gin, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution H182Y of the maturepolypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 186. In another aspect, the aminoacid at a position corresponding to position 186 is substituted withAla, Arg, Asn, Asp, Cys, Gin, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution A186P of the maturepolypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 189. In another aspect, the aminoacid at a position corresponding to position 189 is substituted withAla, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution N189K of the maturepolypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 192. In another aspect, the aminoacid at a position corresponding to position 192 is substituted withAla, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution K192N of the maturepolypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 216. In another aspect, the aminoacid at a position corresponding to position 216 is substituted withAla, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution N216D, N216Q, r N216R,preferably N216D, of the mature polypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 226. In another aspect, the aminoacid at a position corresponding to position 226 is substituted withAla, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution L226K of the maturepolypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 228. In another aspect, the aminoacid at a position corresponding to position 228 is substituted withAla, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution K228E of the maturepolypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 230. In another aspect, the aminoacid at a position corresponding to position 230 is substituted withAla, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution G230H of the maturepolypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 233. In another aspect, the aminoacid at a position corresponding to position 233 is substituted withAla, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution L233H of the maturepolypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 247. In another aspect, the aminoacid at a position corresponding to position 247 is substituted withAla, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution D247N of the maturepolypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 278. In another aspect, the aminoacid at a position corresponding to position 278 is substituted withAla, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution A278S of the maturepolypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 279. In another aspect, the aminoacid at a position corresponding to position 279 is substituted withAla, Arg, Asn, Asp, Cys, Gin, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution G279E of the maturepolypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 281. In another aspect, the aminoacid at a position corresponding to position 281 is substituted withAla, Arg, Asn, Asp, Cys, Gin, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution K281R of the maturepolypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 283. In another aspect, the aminoacid at a position corresponding to position 283 is substituted withAla, Arg, Asn, Asp, Cys, Gin, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution A283D of the maturepolypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 285. In another aspect, the aminoacid at a position corresponding to position 285 is substituted withAla, Arg, Asn, Asp, Cys, Gin, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution N285L, N285M, N285S, N285P,N285T, N285Y, N285H, N285K, N285D, N285W, N285R, or N285G, preferablyN285G, of the mature polypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 289. In another aspect, the aminoacid at a position corresponding to position 289 is substituted withAla, Arg, Asn, Asp, Cys, Gin, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution Q289E of the maturepolypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 292. In another aspect, the aminoacid at a position corresponding to position 292 is substituted withAla, Arg, Asn, Asp, Cys, Gin, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution T292F, T292L, T2921, T292V,T292S, T292P, T292Y, T292Q, T292N, T292K, T292D, T292A, or T292G,preferably T292A, of the mature polypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 294. In another aspect, the aminoacid at a position corresponding to position 294 is substituted withAla, Arg, Asn, Asp, Cys, Gin, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution A294V of the maturepolypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 297. In another aspect, the aminoacid at a position corresponding to position 297 is substituted withAla, Arg, Asn, Asp, Cys, Gin, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution F297L of the maturepolypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 298. In another aspect, the aminoacid at a position corresponding to position 298 is substituted withAla, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution Q298E of the maturepolypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 302. In another aspect, the aminoacid at a position corresponding to position 302 is substituted withAla, Arg, Asn, Asp, Cys, Gin, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution I302D, I302H, I302V, or I302M,preferably I302D, of the mature polypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 311. In another aspect, the aminoacid at a position corresponding to position 311 is substituted withAla, Arg, Asn, Asp, Cys, Gin, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution H311N of the maturepolypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 313. In another aspect, the aminoacid at a position corresponding to position 313 is substituted withAla, Arg, Asn, Asp, Cys, Gin, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution S313D of the maturepolypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 333. In another aspect, the aminoacid at a position corresponding to position 333 is substituted withAla, Arg, Asn, Asp, Cys, Gin, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution W333L of the maturepolypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 346. In another aspect, the aminoacid at a position corresponding to position 346 is substituted withAla, Arg, Asn, Asp, Cys, Gin, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution A346H or A246D of the maturepolypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 353. In another aspect, the aminoacid at a position corresponding to position 353 is substituted withAla, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution T353D of the maturepolypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 386. In another aspect, the aminoacid at a position corresponding to position 386 is substituted withAla, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution A386P of the maturepolypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 387. In another aspect, the aminoacid at a position corresponding to position 387 is substituted withAla, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution I387T of the maturepolypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 388. In another aspect, the aminoacid at a position corresponding to position 388 is substituted withAla, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution K388R of the maturepolypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 390. In another aspect, the aminoacid at a position corresponding to position 390 is substituted withAla, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution K390Q of the maturepolypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 403. In another aspect, the aminoacid at a position corresponding to position 403 is substituted withAla, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution I403Y of the maturepolypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 408 In another aspect, the aminoacid at a position corresponding to position 408 is substituted withAla, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution E408D, E408N, E4085, E408P,E408A, E408G, or E408G, preferably E408D, of the mature polypeptide ofSEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 410. In another aspect, the aminoacid at a position corresponding to position 410 is substituted withAla, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution P410G of the maturepolypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 416. In another aspect, the aminoacid at a position corresponding to position 416 is substituted withAla, Arg, Asn, Asp, Cys, Gin, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution Q416S or Q416D, preferablyQ416S, of the mature polypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 441. In another aspect, the aminoacid at a position corresponding to position 441 is substituted withAla, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution N441G of the maturepolypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 448. In another aspect, the aminoacid at a position corresponding to position 448 is substituted withAla, Arg, Asn, Asp, Cys, Gin, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution A448E, A448W, or A448S,preferably A448E, of the mature polypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 451. In another aspect, the aminoacid at a position corresponding to position 451 is substituted withAla, Arg, Asn, Asp, Cys, Gin, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution K451S or K451Q, preferablyK451S or preferably K451Q, of the mature polypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 471. In another aspect, the aminoacid at a position corresponding to position 471 is substituted withAla, Arg, Asn, Asp, Cys, Gin, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution G471S of the maturepolypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 472. In another aspect, the aminoacid at a position corresponding to position 472 is substituted withAla, Arg, Asn, Asp, Cys, Gin, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution S472Y of the maturepolypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 476. In another aspect, the aminoacid at a position corresponding to position 476 is substituted withAla, Arg, Asn, Asp, Cys, Gin, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution D476R of the maturepolypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 489. In another aspect, the aminoacid at a position corresponding to position 489 is substituted withAla, Arg, Asn, Asp, Cys, Gin, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution Q489P of the maturepolypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 507. In another aspect, the aminoacid at a position corresponding to position 507 is substituted withAla, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution K507R of the maturepolypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 512. In another aspect, the aminoacid at a position corresponding to position 512 is substituted withAla, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution K512P of the maturepolypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 515. In another aspect, the aminoacid at a position corresponding to position 515 is substituted withAla, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution S515V of the maturepolypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 538. In another aspect, the aminoacid at a position corresponding to position 538 is substituted withAla, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution S538C of the maturepolypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 555. In another aspect, the aminoacid at a position corresponding to position 555 is substituted withAla, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution L555Q of the maturepolypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 557. In another aspect, the aminoacid at a position corresponding to position 557 is substituted withAla, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution G557R of the maturepolypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 558. In another aspect, the aminoacid at a position corresponding to position 558 is substituted withAla, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the alteration N558D, N558NP, N558F, N5581,N558E, or N558M of the mature polypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 559. In another aspect, the aminoacid at a position corresponding to position 559 is substituted withAla, Arg, Asn, Asp, Cys, Gin, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution A559S, A559N, A559F, A559M,A559P, A559Y, A559H, A559Q, A559D, or A559G, preferably A559N, of themature polypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 560. In another aspect, the aminoacid at a position corresponding to position 560 is substituted withAla, Arg, Asn, Asp, Cys, Gin, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution S560P and S560G, preferablyS560P, of the mature polypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 561. In another aspect, the aminoacid ata position corresponding to position 561 is substituted with Ala,Arg, Asn, Asp, Cys, Gin, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro,Ser, Thr, Trp, Tyr, or Val. In another aspect, the variant comprises orconsists of the substitution T561P of the mature polypeptide of SEQ IDNO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 564. In another aspect, the aminoacid at a position corresponding to position 564 is substituted withAla, Arg, Asn, Asp, Cys, Gin, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution A564I, A564Y, A564Q, A564E, orA564K, preferably A564I, of the mature polypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 567. In another aspect, the aminoacid at a position corresponding to position 567 is substituted withAla, Arg, Asn, Asp, Cys, Gin, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution V567F or V567P of the maturepolypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 568. In another aspect, the aminoacid at a position corresponding to position 568 is substituted withAla, Arg, Asn, Asp, Cys, Gin, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution K568R of the maturepolypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 570. In another aspect, the aminoacid at a position corresponding to position 570 is substituted withAla, Arg, Asn, Asp, Cys, Gin, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution P570K, P570Q, P570R, P570T,P570S, P570A, P570H, P570G, and P570N, preferably P570K or P570R, of themature polypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 575. In another aspect, the aminoacid at a position corresponding to position 575 is substituted withAla, Arg, Asn, Asp, Cys, Gin, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution I575V of the maturepolypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 579. In another aspect, the aminoacid at a position corresponding to position 579 is substituted withAla, Arg, Asn, Asp, Cys, Gin, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution Y579W or Y579F, preferablyY579W, of the mature polypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 581. In another aspect, the aminoacid at a position corresponding to position 581 is substituted withAla, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution T581M of the maturepolypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 592. In another aspect, the aminoacid at a position corresponding to position 592 is substituted withAla, Arg, Asn, Asp, Cys, Gin, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution G592D of the maturepolypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 593. In another aspect, the aminoacid at a position corresponding to position 593 is substituted withAla, Arg, Asn, Asp, Cys, Gin, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution S593N and S593E, preferablyS593N, of the mature polypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 595. In another aspect, the aminoacid at a position corresponding to position 595 is substituted withAla, Arg, Asn, Asp, Cys, Gin, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution S595L of the maturepolypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 598. In another aspect, the aminoacid at a position corresponding to position 598 is substituted withAla, Arg, Asn, Asp, Cys, Gin, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution S598Q of the maturepolypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 599. In another aspect, the aminoacid at a position corresponding to position 599 is substituted withAla, Arg, Asn, Asp, Cys, Gin, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution A599S of the maturepolypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 602. In another aspect, the aminoacid at a position corresponding to position 602 is substituted withAla, Arg, Asn, Asp, Cys, Gin, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution I602T or I602D, preferablyI602T, of the mature polypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 603. In another aspect, the aminoacid at a position corresponding to position 603 is substituted withAla, Arg, Asn, Asp, Cys, Gin, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution V603P of the maturepolypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 605. In another aspect, the aminoacid at a position corresponding to position 605 is substituted withAla, Arg, Asn, Asp, Cys, Gin, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution S605T of the maturepolypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 607. In another aspect, the aminoacid at a position corresponding to position 607 is substituted withAla, Arg, Asn, Asp, Cys, Gin, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution S607C of the maturepolypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 609. In another aspect, the aminoacid at a position corresponding to position 609 is substituted withAla, Arg, Asn, Asp, Cys, Gin, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution G609E of the maturepolypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 616. In another aspect, the aminoacid at a position corresponding to position 616 is substituted withAla, Arg, Asn, Asp, Cys, Gin, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution S616D or S616G, preferablyS616D, of the mature polypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 627. In another aspect, the aminoacid at a position corresponding to position 627 is substituted withAla, Arg, Asn, Asp, Cys, Gin, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution K627L, K627M, K627V, K627S,K627T, K627Q, or K627R, preferably K627R, of the mature polypeptide ofSEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 630. In another aspect, the aminoacid at a position corresponding to position 630 is substituted withAla, Arg, Asn, Asp, Cys, Gin, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution I630F, I630V, or I630Y of themature polypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 631. In another aspect, the aminoacid at a position corresponding to position 631 is substituted withAla, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution K631A or K631R, preferablyK631R, of the mature polypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 633. In another aspect, the aminoacid at a position corresponding to position 633 is substituted withAla, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution T633V of the maturepolypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 635. In another aspect, the aminoacid at a position corresponding to position 635 is substituted withAla, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution D635P, D635N, D635K, D635E,D635W, D635L, D635M, D635T, D635A, or D635G, preferably D635A, of themature polypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 636. In another aspect, the aminoacid at a position corresponding to position 636 is substituted withAla, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution S636M, S636A, S636H, S636Q,S636N, S636R, S636L, S636H, or S636K, preferably S636N, of the maturepolypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 638. In another aspect, the aminoacid at a position corresponding to position 638 is substituted withAla, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution F638N, F6381, F638V, F638T,F638L, F638Y, F638M or F638H, preferably F638N, of the maturepolypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 639. In another aspect, the aminoacid at a position corresponding to position 639 is substituted withAla, Arg, Asn, Asp, Cys, Gin, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution T639S, T6391, T639M, T639V,T639A, T639D, T639E, T639Y, T639W, T639P, or T639G, preferably T639G orT6391, of the mature polypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 640. In another aspect, the aminoacid at a position corresponding to position 640 is substituted withAla, Arg, Asn, Asp, Cys, Gin, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution T640S of the maturepolypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 642. In another aspect, the aminoacid at a position corresponding to position 642 is substituted withAla, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution S642T or S642N, preferablyS642N, of the mature polypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 643. In another aspect, the aminoacid at a position corresponding to position 643 is substituted withAla, Arg, Asn, Asp, Cys, Gin, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution N643D or N643H, preferablyN643D, of the mature polypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 651. In another aspect, the aminoacid at a position corresponding to position 651 is substituted withAla, Arg, Asn, Asp, Cys, Gin, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution A651P or A651S, preferablyA651P, of the mature polypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 676. In another aspect, the aminoacid at a position corresponding to position 676 is substituted withAla, Arg, Asn, Asp, Cys, Gin, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution D676H of the maturepolypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 683. In another aspect, the aminoacid at a position corresponding to position 683 is substituted withAla, Arg, Asn, Asp, Cys, Gin, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution Q683E of the maturepolypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 688. In another aspect, the aminoacid at a position corresponding to position 688 is substituted withAla, Arg, Asn, Asp, Cys, Gin, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution A688G of the maturepolypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 690. In another aspect, the aminoacid at a position corresponding to position 690 is substituted withAla, Arg, Asn, Asp, Cys, Gin, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution Y690F of the maturepolypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 694. In another aspect, the aminoacid at a position corresponding to position 694 is substituted withAla, Arg, Asn, Asp, Cys, Gin, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution T694A of the maturepolypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 697. In another aspect, the aminoacid at a position corresponding to position 697 is substituted withAla, Arg, Asn, Asp, Cys, Gin, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution T697G of the maturepolypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 698. In another aspect, the aminoacid at a position corresponding to position 698 is substituted withAla, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution R698W of the maturepolypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 699. In another aspect, the aminoacid at a position corresponding to position 699 is substituted withAla, Arg, Asn, Asp, Cys, Gin, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution T699A of the maturepolypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 706. In another aspect, the aminoacid at a position corresponding to position 706 is substituted withAla, Arg, Asn, Asp, Cys, Gin, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution T706Q of the maturepolypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 711. In another aspect, the aminoacid at a position corresponding to position 711 is substituted withAla, Arg, Asn, Asp, Cys, Gin, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution T711S, T711V, or T711Y,preferably T711V, of the mature polypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 713. In another aspect, the aminoacid at a position corresponding to position 713 is substituted withAla, Arg, Asn, Asp, Cys, Gin, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution K713R of the maturepolypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 719. In another aspect, the aminoacid at a position corresponding to position 719 is substituted withAla, Arg, Asn, Asp, Cys, Gin, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution W719R of the maturepolypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 720. In another aspect, the aminoacid at a position corresponding to position 720 is substituted withAla, Arg, Asn, Asp, Cys, Gin, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution K720H of the maturepolypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 744. In another aspect, the aminoacid at a position corresponding to position 744 is substituted withAla, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution K744H or K744Q, preferablyK744H, of the mature polypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 749. In another aspect, the aminoacid at a position corresponding to position 749 is substituted withAla, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution A749T of the maturepolypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 754. In another aspect, the aminoacid at a position corresponding to position 754 is substituted withAla, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution K754R of the maturepolypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 756. In another aspect, the aminoacid at a position corresponding to position 756 is substituted withAla, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution V756Y or V756H, preferablyV756Y, of the mature polypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 760. In another aspect, the aminoacid at a position corresponding to position 760 is substituted withAla, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution S760G of the maturepolypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position , 781. In another aspect, the aminoacid at a position corresponding to position , 781 is substituted withAla, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution T, 781M of the maturepolypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 786. In another aspect, the aminoacid at a position corresponding to position 786 is substituted withAla, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution N786K of the maturepolypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 797. In another aspect, the aminoacid at a position corresponding to position 797 is substituted withAla, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution T797S of the maturepolypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 810. In another aspect, the aminoacid at a position corresponding to position 810 is substituted withAla, Arg, Asn, Asp, Cys, Gin, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution S810Q of the maturepolypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 824. In another aspect, the aminoacid at a position corresponding to position 824 is substituted withAla, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution A824D of the maturepolypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 825. In another aspect, the aminoacid at a position corresponding to position 825 is substituted withAla, Arg, Asn, Asp, Cys, Gin, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution T825G of the maturepolypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 828. In another aspect, the aminoacid at a position corresponding to position 828 is substituted withAla, Arg, Asn, Asp, Cys, Gin, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution N828D of the maturepolypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 833. In another aspect, the aminoacid at a position corresponding to position 833 is substituted withAla, Arg, Asn, Asp, Cys, Gin, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution N833D of the maturepolypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 834. In another aspect, the aminoacid at a position corresponding to position 834 is substituted withAla, Arg, Asn, Asp, Cys, Gin, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution Q834E of the maturepolypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 835. In another aspect, the aminoacid at a position corresponding to position 835 is substituted withAla, Arg, Asn, Asp, Cys, Gin, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution S835A or S835D, preferablyS835A, of the mature polypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 837. In another aspect, the aminoacid at a position corresponding to position 837 is substituted withAla, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution V837I of the maturepolypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 848. In another aspect, the aminoacid at a position corresponding to position 848 is substituted withAla, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution N848D of the maturepolypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 868. In another aspect, the aminoacid at a position corresponding to position 868 is substituted withAla, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution A868E of the maturepolypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 869. In another aspect, the aminoacid at a position corresponding to position 869 is substituted withAla, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution A869V of the maturepolypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 870. In another aspect, the aminoacid at a position corresponding to position 870 is substituted withAla, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution D870V of the maturepolypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 872. In another aspect, the aminoacid at a position corresponding to position 872 is substituted withAla, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution T872G, T872H, T872W, or T872Q,preferably T872G, of the mature polypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 880. In another aspect, the aminoacid at a position corresponding to position 880 is substituted withAla, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution R880K of the maturepolypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 881. In another aspect, the aminoacid at a position corresponding to position 881 is substituted withAla, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution V881Q or V881T, preferablyV881Q, of the mature polypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 883. In another aspect, the aminoacid at a position corresponding to position 883 is substituted withAla, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution T883R, T883V, T883C, or T883K,preferably T883R, of the mature polypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 884. In another aspect, the aminoacid at a position corresponding to position 884 is substituted withAla, Arg, Asn, Asp, Cys, Gin, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution Y884H of the maturepolypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 885. In another aspect, the aminoacid at a position corresponding to position 885 is substituted withAla, Arg, Asn, Asp, Cys, Gin, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution A885Q, A885N, or A885F,preferably A885F, of the mature polypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 887. In another aspect, the aminoacid at a position corresponding to position 887 is substituted withAla, Arg, Asn, Asp, Cys, Gin, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution T887S or T887K, preferablyT887K, of the mature polypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 888. In another aspect, the aminoacid at a position corresponding to position 888 is substituted withAla, Arg, Asn, Asp, Cys, Gin, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution L888M of the maturepolypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 890. In another aspect, the aminoacid at a position corresponding to position 890 is substituted withAla, Arg, Asn, Asp, Cys, Gin, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution V890R of the maturepolypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 892. In another aspect, the aminoacid at a position corresponding to position 892 is substituted withAla, Arg, Asn, Asp, Cys, Gin, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution T892V or T892P, preferablyT892P, of the mature polypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 898. In another aspec, the aminoacid at a position corresponding to position 898 is substituted withAla, Arg, Asn, Asp, Cys, Gin, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution R898Q of the maturepolypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 905. In another aspect, the aminoacid at a position corresponding to position 905 is substituted withAla, Arg, Asn, Asp, Cys, Gin, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution N905D of the maturepolypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 906. In another aspect, the aminoacid at a position corresponding to position 906 is substituted withAla, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution F906A of the maturepolypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 912. In another aspect, the aminoacid at a position corresponding to position 912 is substituted withAla, Arg, Asn, Asp, Cys, Gin, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution Q912V of the maturepolypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 920. In another aspect, the aminoacid at a position corresponding to position 920 is substituted withAla, Arg, Asn, Asp, Cys, Gin, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution N920D or N920P of the maturepolypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 921. In another aspect, the aminoacid at a position corresponding to position 921 is substituted withAla, Arg, Asn, Asp, Cys, Gin, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution K921R or K921E of the maturepolypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 924. In another aspect, the aminoacid at a position corresponding to position 924 is substituted withAla, Arg, Asn, Asp, Cys, Gin, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution A924D of the maturepolypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 926. In another aspect, the aminoacid at a position corresponding to position 926 is substituted withAla, Arg, Asn, Asp, Cys, Gin, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution V926F or V926P, preferablyV926P, of the mature polypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 927. In another aspect, the aminoacid at a position corresponding to position 927 is substituted withAla, Arg, Asn, Asp, Cys, Gin, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution K927R of the maturepolypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 928. In another aspect, the aminoacid at a position corresponding to position 928 is substituted withAla, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution S928D of the maturepolypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 932. In another aspect, the aminoacid at a position corresponding to position 932 is substituted withAla, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution T932A of the maturepolypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 933. In another aspect, the aminoacid at a position corresponding to position 933 is substituted withAla, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution N933S or N933V, preferablyN933S, of the mature polypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 934. In another aspect, the aminoacid at a position corresponding to position 934 is substituted withAla, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution Y934G, Y034R, or Y934Q,preferably Y934G, of the mature polypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 937. In another aspect, the aminoacid at a position corresponding to position 937 is substituted withAla, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution A937E of the maturepolypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 938. In another aspect, the aminoacid at a position corresponding to position 938 is substituted withAla, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution V938I of the maturepolypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 939. In another aspect, the aminoacid at a position corresponding to position 939 is substituted withAla, Arg, Asn, Asp, Cys, Gin, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution K939V of the maturepolypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 941. In another aspect, the aminoacid at a position corresponding to position 941 is substituted withAla, Arg, Asn, Asp, Cys, Gin, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution N941S of the maturepolypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 942. In another aspect, the aminoacid at a position corresponding to position 942 is substituted withAla, Arg, Asn, Asp, Cys, Gin, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution A942P of the maturepolypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 946. In another aspect, the aminoacid at a position corresponding to position 946 is substituted withAla, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution G946R of the maturepolypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 948. In another aspect, the aminoacid at a position corresponding to position 948 is substituted withAla, Arg, Asn, Asp, Cys, Gin, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution K948R of the maturepolypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 956. In another aspect, the aminoacid at a position corresponding to position 956 is substituted withAla, Arg, Asn, Asp, Cys, Gin, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution Q956Y or A956S, preferablyQ956Y, of the mature polypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 957. In another aspect, the aminoacid at a position corresponding to position 957 is substituted withAla, Arg, Asn, Asp, Cys, Gin, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution A957L or A957P, preferablyA957L, of the mature polypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 966. In another aspect, the aminoacid at a position corresponding to position 966 is substituted withAla, Arg, Asn, Asp, Cys, Gin, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution N966C of the maturepolypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 972. In another aspect, the aminoacid at a position corresponding to position 972 is substituted withAla, Arg, Asn, Asp, Cys, Gin, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution T972K of the maturepolypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 980. In another aspect, the aminoacid at a position corresponding to position 980 is substituted withAla, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution M980I of the maturepolypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 994. In another aspect, the aminoacid at a position corresponding to position 994 is substituted withAla, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution G994N or G994D of the maturepolypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 999. In another aspect, the aminoacid at a position corresponding to position 999 is substituted withAla, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution T999R of the maturepolypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 1011. In another aspect, the aminoacid at a position corresponding to position 1011 is substituted withAla, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution L1011A of the maturepolypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 1031. In another aspect, the aminoacid at a position corresponding to position 1031 is substituted withAla, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution K1031I of the maturepolypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 1037. In another aspect, the aminoacid at a position corresponding to position 1037 is substituted withAla, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution A1037E of the maturepolypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 1038. In another aspect, the aminoacid at a position corresponding to position 1038 is substituted withAla, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution S1038G of the maturepolypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 1041. In another aspect, the aminoacid at a position corresponding to position 1041 is substituted withAla, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution G1041R of the maturepolypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 1042. In another aspect, the aminoacid at a position corresponding to position 1042 is substituted withAla, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution Y1042N of the maturepolypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 1048. In another aspect, the aminoacid at a position corresponding to position 1048 is substituted withAla, Arg, Asn, Asp, Cys, Gin, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution F1048W of the maturepolypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of alterations atpositions corresponding to positions 559+579. In another aspect, theamino acids at positions corresponding to positions 559+579 areidependently substituted with Ala, Arg, Asn, Asp, Cys, Gin, Glu, Gly,His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val. Inanother aspect, the variant comprises or consists of the substitutionsA559N+Y579W or A559N+Y579F of the mature polypeptide of SEQ ID NO:2.

In another aspect, the variant comprises or consists of an alterationata position corresponding to position selected from the groupconsisting of alterations in positions: 17, 20, 51, 53, 55, 56, 60, 63,79, 87, 192, 302, 387, 388, 390, 403, 408, 410, 416, 448, 451, 471, 472,507, 512, 515, 538, 598, 602, 605, 609, 676, 694, 698, 699, 711, 754,760, , 781, 786, 797, 834, and 835 of SEQ ID NO:2. In another aspect,the amino acid at a position corresponding to any of positions asdescribed above is substituted with Ala, Arg, Asn, Asp, Cys, Gin, Glu,Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val. Inanother aspect, the variant comprises or consists of the substitutionselected from the group consisting of: S17A, F20P, F20N, F20G, F20Y,K51Q, K51H, E53P, E53G, Y55M, V56M, Y60F, S63F, T87R, K192N, I302H,I302V, I302M, I387T, K388R, K390Q, I403Y, E408D, E4085, E408P, E408A,E408G, E408N, P410G, Q4165, Q416D, A448E, A448W, A4485, K451S, G471S,S472Y, K507R, K512P, S515V, S538C, Y579W, S598Q, I602T, I602D, S605T,G609E, D676H, T694A, R698W, T699A, T711V, T711Y, K754R, S760G, T, 781M,N786K, T797S, Q834E, and S835D, wherein numbering is according to SEQ IDNO:2.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position selected from the group consistingof alterations in positions: 4, 17, 18, 20, 51, 53, 55, 56, 60, 63, 71,79, 87, 92, 99, 120, 125, 126, 130, 137, 182, 186, 189, 192, 213, 216,221, 226, 228, 230, 231, 232, 233, 235, 240, 243, 247, 249, 278, 279,281, 283, 285, 289, 292, 294, 298, 302, 311, 313, 333, 346, 353, 358,386, 387, 388, 390, 403, 408, 410, 416, 441, 448, 451, 471, 472, 476,489, 507, 512, 515, 538, 555, 556, 557, 558, 559, 560, 561, 562, 563,564, 567, 568, 570, 575, 578, 579, 580, 581, 583, 589, 590, 591, 592,593, 595, 598, 599, 602, 603, 605, 607, 609, 616, 627, 630, 631, 635,636, 638, 639, 640, 641, 642, 643, 644, 651, 676, 683, 688, 690, 694,698, 699, 706, 711, 713, 1719, 720, 744, 749, 754, 756, 760, , 781, 786,797, 810, 811, 812, 815, 823, 824, 825, 827, 828, 833, 834, 835, 837,843, 848, 868, 869, 870, 871, 872, 873, 874, 880, 881, 883, 884, 885,887, 888, 890, 892, 894, 898, 905, 906, 912, 920, 921, 924, 926, 927,928, 932, 933, 934, 935, 937, 938, 939, 940, 941, 942, 943, 946, 948,950, 952, 953, 954, 956, 957, 960, 966, 971, 972, 980, 989, 991, 994,995, 998, 999, 1006, 1009, 1010, 1011, 1029, 1030, 1031, 1032, 1035,1037, 1038, 1040, 1041, 1042, 1044, 1045, 1048 of SEQ ID NO:2.

In another aspect, the amino acid at a position corresponding to any ofpositions as described above is substituted with Ala, Arg, Asn, Asp,Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp,Tyr, or Val. In another aspect, the variant comprises or consists of thesubstitution selected from the group consisting of: N285G, W333L, T353D,N558NP, N558F, T633V, D635L, D635M, D635T, F638Y, T639D, G994N, andK281T, G563E, 1575M, 1575A, K921D, N558K+A559K+S560F+T561P+G562W, N558K,A559K, S560F, T561P, G562W and I125V, A126R, K130R, K213R, A221R, K228E,K2281, G230F, G230L, G230A, G230H, G230N, G230W, G230T, F231Y, F231N,V232R, V232G, H235D, N240Q, G243K, G243R, A249N, A278S, K281F, K281V,K281Y, K281H, K281Q, K281N, K281W, N285L, N285M, N285S, N285P, N285T,N285Y, N285H, N285K, N285D, N285W, N285R, T292F, T292L, T2921, T292V,T292S, T292P, T292Y, T292Q, T292N, T292K, T292D, T292G, F297L, A346H,G556S, N558D, N558M, N558Q, N5581, N558Y, N558H, A559N, A559F, A559M,A559P, A559Y, A559H, A559Q, A559D, A559R, A559G, A559I, A559S, S560P,S560K, S560G, S560D, T561P, T561E, T561Q, T561S, T561D, A5641, A564Y,A564H, A564Q, A564K, A564E, E565M, V567F, K568R, L569F, L569Y, L569D,L569E, P570F, P570L, P5701, P570M, P570V, P570S, P570T, P570A, P570Y,P570H, P570Q, P570N, P570K, P570E, P570W, P570R, P570G, I575D, I575E,I576F, I576M, I576P, D578R, Y579F, Y579W, V580L, D583M, Q589G, P590S,P590T, P590E, E591L, G592D, S593P, S593H, S593Q, S593N, S593K, S593D,S593E, S593R, S616D, K627L, K627M, K627V, K627S, K627T, K627Q, K627R,I630F, I630V, I630Y, D635A, D635P, D635N, D635K, D635E, D635G, D635W,S636L, S636M, S636A, S636H, S636Q, S636N, S636K, S636R, F638I, F638V,F638T, F638L, F638H, T639V, T639S, T639L, T639I, T639M, T639A, T639E,T639W, T639G, Y641E, S642T, S642N, N643D, N643H, N643T, T644F, A651P,S810R, A811S, V812F, V812I, V812M, V812W, V812R, N815V, N815Y, N815E,N815W, N815R, S823Q, A824T, T825N, T825W, T825A, T825D, V827I, V827M,V827S, T843V, D870F, D870L, D870I, D870M, D870V, D870S, D870T, D870Y,D870H, D870Q, D870N, D870K, D870E, D870W, D870R, D870G, P871F, P871L,P871I, P871M, P871V, P871S, P871T, P871A, P871Y, P871H, P871Q, T872S,T872F, T872A, T872Y, T872H, T872Q, T872N, T872K, T872D, T872E, T872W,T872R, T872G, D873K, D873E, T874V, T874S, T874P, T874A, T874H, T874Q,T874N, T874K, V881Q, T883K, Y884H, A885F, A885Q, A885N, T887L, T8871,T887S, T887H, T887R, K894E, N920D, K921R, K921E, T932A, N933V, N933S,Y934G, Y934M, Y934S, Y934A, Y934Q, Y934N, Y934E, Y934W, Y934R, T935W,A937F, A937V, A937S, A937T, A937Q, A937D, A937E, V938I, K939I, K939V,D940E, N941S, N941H, N941D, A942P, A942E, D943Y, D943H, R950V, R950H,R950N, F952S, F952W, N953Y, G954L, Y960F, A964N, A964C, N966P, N966C,G971A, Q974K, Q974C, Q9891, Q991L, Q991I, Q991M, Q991V, Q991T, Q991K,Q991C, S995I, S995V, S995Q, S995R, S995C, G998V, G998A, 51006T, 51006A,S1006K, 51006R, Y1010W, L1011M, L1011S, L1011A, L1011Q, L1011N, L1011D,L1011E, R1029N, F1030M, K1031I, K1031S, K1031T, K1031H, V1032G, K1035A,A1037E, A1037W, 51038L, S10381, L1040N, L1040E, G1041F, L1044F, L1044S,L1044N, L1044W, P1045Q, P1045W, and A559N+Y579F, A559N, Y579F,A564E+Y579F, A564E, Y579F, A559N+Y579W, A559N, Y579W, G562P+Y579W,G562P, Y579W, A564D+Y579W, A564D, Y579W, A559N+Y579W+K99R, A559N, Y579W,K99R, A559N+Y579W+K281R, A559N, Y579W, K281R, K281R+A559N+Y579W, K281R,A559N, Y579W, A559N+Y579W+S616D, A559N, Y579W, S616D, A559N+Y579W+S636N,A559N, Y579W, S636N, A559N+Y579W+A651P, A559N, Y579W, A651P,A559N+Y579W+K948E, A559N, Y579W, K948E, A559N+Y579W+K1009E, A559N,Y579W, K1009E, A559N+Y579W+K627R, A559N, Y579W, K627R, Y579W+K921R,Y579W, K921R, A559N+Y579W+K921R, A559N, Y579W, K921R, K99R+Y579W, K99R,Y579W, Y579W+A651P, Y579W, A651P, Y579W+K948E, Y579W, K948E,Y579W+K1009E, Y579W, K1009E, A559N+Y579W+Y934G, A559N, Y579W, Y934G,A559N+Y579W+K921R+Y934G, A559N, Y579W, K921R, Y934G, A559N+Y579W+K627M,A559N, Y579W, K627M, A559N+Y579W+K627R+S616D, A559N, Y579W, K627R,S616D, A559N+Y579F+K627R, A559N, Y579F, K627R, A559N+Y579W+K921R+A651P,A559N, Y579W, K921R, A651P, A559N+Y579W+K921R+K627R, A559N, Y579W,K921R, K627R, A559N+Y579W+K921R+S636K, A559N, Y579W, K921R, S636K,A559N+Y579W+K921R+S616D, A559N, Y579W, K921R, S616D,A559N+Y579W+K921R+S636N, A559N, Y579W, K921R, S636N,A559N+Y579W+K921R+K627R+S636N, A559N, Y579W, K921R, K627R, S636N,A559N+Y579W+S636N+A651P, A559N, Y579W, S636N, A651P,A559N+Y579W+S616D+A651P, A559N, Y579W, S616D, A651P,A559N+Y579W+S616D+S636K, A559N, Y579W, S616D, S636K,A559N+Y579W+S616D+K921R+Y934G, A559N, Y579W, S616D, K921R, Y934G,A559N+Y579W+A651P+K627M, A559N, Y579W, A651P, K627M,A559N+Y579W+A651P+S636K, A559N, Y579W, A651P, S636K,A559N+Y579W+A651P+K627R+S636N, A559N, Y579W, A651P, K627R, S636N,A559N+Y579W+A651P+S616D, A559N, Y579W, A651P, S616D,A559N+Y579W+A651P+K921R+Y934G, A559N, Y579W, A651P, K921R, Y934G,S636N+Y934G, S636N, Y934G, S636N+K921R, S636N, K921R, S636N+K627R,S636N, K627R, S636N+Y579W, S636N, Y579W, F6381+Y934G, F6381, Y934G,F638I+K921R, F638I, K921R, F638I+K627R, F638I, K627R, F638I+Y579W,F638I, Y579W, K627R+K51Q, K627R, K51Q, K627R+K451S, K627R, K451S,K627R+A559N, K627R, A559N, K627R+Y579W, K627R, Y579W, Y579W+Y934G,Y579W, Y934G, A651P+F638I, A651P, F638I, P570Q+A651P, P570Q, A651P,P570Q+K921R, P570Q, K921R, P570Q+K627R, P570Q, K627R, P570Q+A559N,P570Q, A559N, P570Q+Y579W, P570Q, Y579W, P570Q+F638I, P570Q, F638I,P570K+Y579W, P570K, Y579W, P570K+F638I, P570K, F638I, P570T+A651P,P570T, A651P, P570T+S636N, P570T, S636N, P570T+Y934G, P570T, Y934G,P570T+F638I, P570T, F638I, P570T+K921R, P570T, K921R, P570T+K627R,P570T, K627R, P570T+A559N, P570T, A559N, P570T+A885F, P570T, A885F,A885F+Y934G, A885F, Y934G, A885F+K627R, A885F, K627R, A559N+Y579W+S636L,A559N, Y579W, S636L, A559N+Y579W+F638I, A559N, Y579W, F6381I,A559N+Y579W+D870M, D870M, A559N+Y579W+S560P, S560P, A559N+Y579W+A564I,A564I, A559N+Y579W+P570N, P570N, A559N+Y579W+P570K, P570K,A559N+Y579W+P570R, P570R, A559N+Y579W+P570A, P570A, A559N+Y579W+P570T,P570T, A559N+Y579W+P570S, P570S, A559N+Y579W+P570Q, P570Q,A559N+Y579W+P570H, P570H, and N558E, A559P, A559N, A559H, T561P, A564E,P570A, P570Q, P570R, P570S, P570K, P570T, P570N, Y579W, Y579F, T581M,S616D, K627R, K627M, K627Q, S636N, S636Q, S636R, S636K, S636M, 5636H,F6381, F638L, N643D, A651P, A651S, A885F, A885Q, K921R, Y934R, Y934G,N966C, L1011A, K1031I, and A559N+P570A+Y579W, A559N+P570H+Y579W,A559N+P570K+Y579W, A559N+P570N+Y579W, A559N+P570Q+Y579W,A559N+P570R+Y579W, A559N+P570S+Y579W, A559N+P570T+Y579W,A559N+S560P+Y579W, A559N+Y579W+A651P, A559N+Y579W+A651P+Y934G,A559N+Y579W+F6381, A559N+Y579W+K921R, A559N+Y579W+S616D+K921R,A559N+Y579W+S636N, A559N+Y579F, A559N+Y579W, A559N+Y579W+K921R,A559N+Y579W+S616D, F638I+Y934G, K627R+S636N, K627R+Y934G, P570K+Y579W,Q416D+A559N+Y579W+S636N, Q416D, S128X+A559N+Y579W+K627R, S128X,S128X+A559N+Y579W+S636N, Y579W+S636N, V4T, S17A, N18G, F20P, F20N, F20G,F20Y, K51Q, K51H, E53Y, E53P, E53G, Y55M, Y55D, V56M, Y60F, S63F, A71E,579W, T87R, T92S, A120P, N129D, F137L, H182Y, A186P, N189K, K192N,N216D, N216Q, N216R, L226K, G230H, L233H, D247N, G279E, K281R, A283D,N285D, N285G, Q289E, T292A, T292F, T292Y, A294V, Q298E, I302D, I302H,I302V, I302M, H311N, S313D, A346D, A386P, I387T, K388R, K390Q, I403Y,E408D, E408N, E4085, E408P, E408A, E408G, P410G, Q4165, Q416D, N441G,A448E, A448W, A4485, K451S, K451Q, G471S, S472Y, D476R, Q489P, K507R,K512P, S515V, S538C, L555Q, G557R, N558E, A559N, A559P, A559H, A559D,S560P, S560G, T561P, A564E, A564I, V567P, K568R, P570R, P570Q, P570K,P570A, P570T, P570G, P570S, P570H, P570N, I575V, Y579W, Y579F, T581M,S593N, S593E, S595L, S598Q, A599S, I602T, I602D, V603P, S605T, S607C,G609E, S616G, S616D, K627R, K627M, K627Q, K631R, K631A, D635A, D635E,D635M, D635N, D635L, D635W, S636N, S636K, S636L, S636Q, S636R, S636M,5636H, F638N, F638I, F638L, F638V, F638H, F638M, T639G, T639I, T639M,T639Y, T639W, T639P, T639E, T640S, S642N, S642T, N643D, N643H, A651P,A651S, D676H, Q683E, A688G, Y690F, T694A, T697G, R698W, T699A, T706Q,T711S, T711V, T711Y, K713R, W719R, K720H, K744H, K744Q, A749T, K754R,V756Y, V756H, 5760G, T, 781M, N786K, T797S, S810Q, A824D, T825G, N828D,N833D, Q834E, S835A, S835D, V8371, N848D, A868E, A869V, D870V, T872G,T872H, T872W, T872Q, R880K, V881Q, V881T, T883R, T883V, T883C, T883K,Y884H, A885N, A885Q, A885F, T887K, T887S, L888M, V890R, T892P, T892V,R898Q, N905D, F906A, Q912V, N920P, K921R, A924D, V926F, V926P, K927R,S928D, T932A, N933S, N933V, Y934G, Y934R, Y934Q, A937E, V9381, K939V,N941S, A942P, G946R, K948R, Q956Y, Q956S, A957L, A957P, N966C, T972K,M9801, G994D, T999R, L1011A, K1031I, A1037E, S1038G, G1041R, Y1042N, andF1048W, wherein numbering is according to SEQ ID NO:2.

In another aspect, a variant comprises an alteration at one or more(e.g. several) positions corresponding to positions 17, 20, 51, 53, 55,56, 60, 63, 79, 87, 192, 302, 387, 388, 390, 403, 408, 410, 416, 448,451, 471, 472, 507, 512, 515, 538, 598, 602, 605, 609, 676, 694, 698,699, 711, 754, 760, , 781, 786, 797, 834, and 835 of SEQ ID NO:2.

In another aspect, a variant comprises an alteration at two positionscorresponding to any of positions positions 17, 20, 51, 53, 55, 56, 60,63, 79, 87, 192, 302, 387, 388, 390, 403, 408, 410, 416, 448, 451, 471,472, 507, 512, 515, 538, 598, 602, 605, 609, 676, 694, 698, 699, 711,754, 760, , 781, 786, 797, 834, and 835 of SEQ ID NO:2.

In another aspect, a variant comprises an alteration at three positionscorresponding to any of positions positions 17, 20, 51, 53, 55, 56, 60,63, 79, 87, 192, 302, 387, 388, 390, 403, 408, 410, 416, 448, 451, 471,472, 507, 512, 515, 538, 598, 602, 605, 609, 676, 694, 698, 699, 711,754, 760, , 781, 786, 797, 834, and 835 of SEQ ID NO:2. Thus, in oneembodiment, the variant comprises an alteration in the positionscorresponding to: 17+20, 17+51, 17+53, 17+55, 17+56, 17+60, 17+63,17+79, 17+87, 17+192, 17+302, 7+387, 17+388, 17+390, 17+403, 17+408,17+410, 17+416, 17+448, 17+451, 17+471, 17+472, 17+507, 17+512, 17+515,17+538, 17+598, 17+602, 17+605, 17+609, 17+676, 17+694, 17+698, 17+699,17+711, 17+754, 17+760, 17+781, 17+786, 17+797, 17+834, 17+835, 20+51,20+53, 20+55, 20+56, 20+60, 20+63, 20+79, 20+87, 20+192, 20+302, 20+387,20+388, 20+390, 20+403, 20+408, 20+410, 20+416, 20+448, 20+451, 20+471,20+472, 20+507, 20+512, 20+515, 20+538, 20+598, 20+602, 20+605, 20+609,20+676, 20+694, 20+698, 20+699, 20+711, 20+754, 20+760, 20+781, 20+786,20+797, 20+834, 20+835, 51+53, 51+55, 51+56, 51+60, 51+63, 51+79, 51+87,51+192, 51+302, 51+387, 51+388, 51+390, 51+403, 51+408, 51+410, 51+416,51+448, 51+451, 51+471, 51+472, 51+507, 51+512, 51+515, 51+538, 51+598,51+602, 51+605, 51+609, 51+676, 51+694, 51+698, 51+699, 51+711, 51+754,51+760, 51+781, 51+786, 51+797, 51+834, 51+835, 53+55, 53+56, 53+60,53+63, 53+79, 53+87, 53+192, 53+302, 53+387, 53+388, 53+390, 53+403,53+408, 53+410, 53+416, 53+448, 53+451, 53+471, 53+472, 53+507, 53+512,53+515, 53+538, 53+598, 53+602, 53+605, 53+609,3+676, 53+694, 53+698,53+699, 53+711, 53+754, 53+760, 53+781, 53+786, 53+797, 53+834, 53+835,55+56, 55+60, 55+63, 55+79, 55+87, 55+192, 55+302, 55+387, 55+388,55+390, 55+403, 55+408, 55+410, 55+416, 55+448, 55+451, 55+471, 55+472,55+507, 55+512, 55+515, 55+538, 55+598, 55+602, 55+605, 55+609, 55+676,55+694, 55+698, 55+699, 55+711, 55+754, 55+760, 55+781, 55+786, 55+797,55+834, 55+835, 56+60, 56+63, 56+79, 56+87, 56+192, 56+302, 56+387,56+388, 56+390, 56+403, 56+408, 56+410, 56+416, 56+448, 56+451, 56+471,56+472, 56+507, 56+512, 56+515, 56+538, 56+598, 56+602, 56+605, 56+609,56+676, 56+694, 56+698, 56+699, 56+711, 56+754, 56+760, 56+781, 56+786,56+797, 56+834, 56+835, 60+63, 60+79, 60+87, 60+192, 60+302, 60+387,60+388, 60+390, 60+403, 60+408, 60+410, 60+416, 60+448, 60+451, 60+471,60+472, 60+507, 60+512, 60+515, 60+538, 60+598, 60+602, 60+605, 60+609,60+676, 60+694, 60+698, 60+699, 60+711, 60+754, 60+760, 60+781, 60+786,60+797, 60+834, 60+835, 63+79, 63+87, 63+192, 63+302, 63+387, 63+388,63+390, 63+403, 63+408, 63+410, 63+416, 63+448, 63+451, 63+471, 63+472,63+507, 63+512, 63+515, 63+538, 63+598, 63+602, 63+605, 63+609, 63+676,63+694, 63+698, 63+699, 63+711, 63+754, 63+760, 63+781, 63+786, 63+797,63+834, 63+835, 79+87, 79+192, 79+302, 79+387, 79+388, 79+390, 79+403,79+408, 79+410, 79+416, 79+448, 79+451, 79+471, 79+472, 79+507, 79+512,79+515, 79+538, 79+598, 79+602, 79+605, 79+609, 79+676, 79+694, 79+698,79+699, 79+711, 79+754, 79+760, 79+781, 79+786, 79+797, 79+834, 79+835,87+192, 87+302, 87+387, 87+388, 87+390, 87+403, 87+408, 87+410, 87+416,87+448, 87+451, 87+471, 87+472, 87+507, 87+512, 87+515, 87+538, 87+598,87+602, 87+605, 87+609, 87+676, 87+694, 87+698, 87+699, 87+711, 87+754,87+760, 87+781, 87+786, 87+797, 87+834, 87+835, 192+302, 192+387,192+388, 192+390, 192+403, 192+408, 192+410, 192+416, 192+448, 192+451,192+47t192+472, 192+507, 192+512, 192+515, 192+538, 192+598, 192+602,192+605, 192+609, 192+676, 192+694, 192+698, 192+699, 192+711, 192+754,192+760, 192+78t192+786, 192+797, 192+834, 192+835, 302+387, 302+388,302+390, 302+403, 302+408, 302+410, 302+416, 302+448, 302+451, 302+471,302+472, 302+507, 302+512, 302+515, 302+538, 302+598, 302+602, 302+605,302+609, 302+676, 302+694, 302+698, 302+699, 302+711, 302+754, 302+760,302+781, 302+786, 302+797, 302+834, 302+835, 387+388, 387+390, 387+403,387+408, 387+410, 387+416, 387+448, 387+451, 387+471, 387+472, 387+507,387+512, 387+515, 387+538, 387+598, 387+602, 387+605, 87+609, 387+676,387+694, 387+698, 387+699, 387+711387+754, 387+760, 387+781, 387+786,387+797, 387+834, 387+835, 388+390, 388+403, 388+408, 388+410, 388+416,388+448, 388+451, 388+471, 388+472, 388+507, 388+512, 388+515, 388+538,388+598, 388+602, 388+605, 388+609, 388+676, 388+694, 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605+698+754,605+698+760, 605+698+781, 605+698+786, 605+698+797, 605+698+834,605+698+835, 605+699+711, 605+699+754, 605+699+760, 605+699+781,605+699+786, 605+699+797, 605+699+834, 605+699+835, 605+711+754,605+711+760, 605+711+781, 605+711+786, 605+711+797, 605+711+834,605+711+835, 605+754+760, 605+754+781, 605+754+786, 605+754+797,605+754+834, 605+754+835, 605+760+781, 605+760+786, 605+760+797,605+760+834, 605+760+835, 605+781+786, 605+781+797, 605+781+834,605+781+835, 605+786+797, 605+786+834, 605+786+835, 605+797+834,605+797+835, 605+834+835, 609+676+694, 609+676+698, 609+676+699,609+676+711, 609+676+754, 609+676+760, 609+676+781, 609+676+786,609+676+797, 609+676+834, 609+676+835, 609+694+698, 609+694+699,609+694+711, 609+694+754, 609+694+760, 609+694+781, 609+694+786,609+694+797, 609+694+834, 609+694+835, 609+698+699, 609+698+711,609+698+754, 609+698+760, 609+698+781, 609+698+786, 609+698+797,609+698+834, 609+698+835, 609+699+711, 609+699+754, 609+699+760,609+699+781, 609+699+786, 609+699+797, 609+699+834, 609+699+835,609+711+754, 609+711+760, 609+711+781, 609+711+786, 609+711+797,609+711+834, 609+711+835, 609+754+760, 609+754+781, 609+754+786,609+754+797, 609+754+834, 609+754+835, 609+760+781, 609+760+786,609+760+797, 609+760+834, 609+760+835, 609+781+786, 609+781+797,609+781+834, 609+781+835, 609+786+797, 609+786+834, 609+786+835,609+797+834, 609+797+835, 609+834+835, 676+694+698, 676+694+699,676+694+711, 676+694+754, 676+694+760, 676+694+781, 676+694+786,676+694+797, 676+694+834, 676+694+835, 676+698+699, 676+698+711,676+698+754, 676+698+760, 676+698+781, 676+698+786, 676+698+797,676+698+834, 676+698+835, 676+699+711, 676+699+754, 676+699+760,676+699+781, 676+699+786, 676+699+797, 676+699+834, 676+699+835,676+711+754, 676+711+760, 676+711+781, 676+711+786, 676+711+797,676+711+834, 676+711+835, 676+754+760, 676+754+781, 676+754+786,676+754+797, 676+754+834, 676+754+835, 676+760+781, 676+760+786,676+760+797, 676+760+834, 676+760+835, 676+781+786, 676+781+797,676+781+834, 676+781+835, 676+786+797, 676+786+834, 676+786+835,676+797+834, 676+797+835, 676+834+835, 694+698+699, 694+698+711,694+698+754, 694+698+760, 694+698+781, 694+698+786, 694+698+797,694+698+834, 694+698+835, 694+699+711, 694+699+754, 694+699+760,694+699+781, 694+699+786, 694+699+797, 694+699+834, 694+699+835,694+711+754, 694+711+760, 694+711+781, 694+711+786, 694+711+797,694+711+834, 694+711+835, 694+754+760, 694+754+781, 694+754+786,694+754+797, 694+754+834, 694+754+835, 694+760+781, 694+760+786,694+760+797, 694+760+834, 694+760+835, 694+781+786, 694+781+797,694+781+834, 694+781+835, 694+786+797, 694+786+834, 694+786+835,694+797+834, 694+797+835, 694+834+835, 698+699+711, 698+699+754,698+699+760, 698+699+781, 698+699+786, 698+699+797, 698+699+834,698+699+835, 968+711+754, 698+711+760, 698+711+781, 698+711+786,698+711+797, 698+711+834, 698+711+835, 698+754+760, 698+754+781,698+754+786, 698+754+797, 698+754+834, 698+754+835, 698+760+781,698+760+786, 698+760+797, 698+760+834, 698+760+835, 698+781+786,698+781+797, 698+781+834, 698+781+835, 698+786+797, 698+786+834,698+786+835, 698+797+834, 698+797+835, 698+834+835, 699+711+754,699+711+760, 699+711+781, 699+711+786, 699+711+797, 699+711+834,699+711+835, 699+754+760, 699+754+781, 699+754+786, 699+754+797,699+754+834, 699+754+835, 699+760+781, 699+760+786, 699+760+797,699+760+834, 699+760+835, 699+781+786, 699+781+797, 699+781+834,699+781+835, 699+786+797, 699+786+834, 699+786+835, 699+797+834,699+797+835, 699+834+835, 711+754+760, 711+754+781, 711+754+786,711+754+797, 711+754+834, 711+754+835, 711+760+781, 711+760+786,711+760+797, 711+760+834, 711+760+835, 711+781+786, 711+781+797,711+781+834, 711+781+835, 711+786+797, 711+786+834, 711+786+835,711+797+834, 711+797+835, 711+834+835, 754+760+781, 754+760+786,754+760+797, 754+760+834, 754+760+835, 754+781+786, 754+781+797,754+781+834, 754+781+835, 754+786+797, 754+786+834, 754+786+835,754+797+834, 754+797+835, 754+834+835, 760+781+786, 60+781+797,760+781+834, 760+781+835, 760+786+797, 760+786+834, 760+786+835,760+797+834, 760+797+835 760+834+835, 781+786+797,, 781+786+834,,781+786+835,, 781+797+834,, 781+797+835,, 781+834+835, 786+797+834,786+797+835, 786+834+835, and 797+834+835 of SEQ ID NO:2.

The concrete substitutions in the positions indicated in the above listare preferably those described above in relation to the individualpositions.

Preferred are endoglucanase variants having an alteration (e.g. asubstitution, deletion or insertion, preferably a substitution) in thepositions selected from the group consisting of positions: 559+579+697;512+559+579+697; 18+71+186+408+579+602+651+688+756;18+189+408+559+579+688+697+756+921+934; 313+488; 880+905+921+934;302+313+408+579+602+651+697+880+921+934; or216+313+408+476+579+602+638+651+697+719+880+887+921+934 of SEQ ID NO:2.

Particularly preferred are endoglucanase variants having an alteration(e.g. a substitution, deletion or insertion, preferably a substitution)selected from the group consisting of the following alterations:A559N+Y579W+T697G; K512P+A559N+Y579W+T697G;N18G+A71E+A186P+E408D+Y579W+I602T+A651P+A688G+V756Y;N18G+N189K+E408D+A559N+Y579W+A688G+T697G+V756Y+K921R+Y934G; S313D+E408D;R880K+N905D+K921R+Y934G;I302D+S313D+E408D+Y579W+I602T+A651P+T697G+R880K+K921R+Y934G; andN216Q+S313D+E408D+D476R+Y579W+I602T+F638N+A651P+T697G+W719R+R880K+T887K+K921R+Y934G.

All of the above alterations are relative to SEQ ID NO:2.

In a particular embodiment, the invention relates to a detergentcomposition comprising an endoglucanase variant selected from the groupconsisting of the endoglucananase variants set forth in Tables 2-12 or13 herein.

In a particular embodiment, the invention relates to a detergentcomposition comprising an endoglucananase variant selected from thegroup consisting of the endoglucananase variants set forth in Table 14herein.

In a particular embodiment, the invention relates to a detergentcomposition comprising an endoglucananase variant selected from thegroup consisting of the endoglucananase variants set forth in Table 15herein.

In a particular embodiment, the invention relates to a detergentcomposition comprising an endoglucananase variant selected from thegroup consisting of the endoglucananase variants set forth in Table 16herein.

In one embodiment, the chelator-induced instability regions in theprotein sequence of the known xanthan lyase having SEQ ID NO:6 that areaffected when the molecule is incubated in a buffer with EDTA, are thefollowing: region 1 corresponding to amino acids 154 to 176 of SEQ IDNO:6, region 2 corresponding to amino acids 614 to 658 of SEQ ID NO:6,region 3 corresponding to amino acids 731 to 803 of SEQ ID NO:6, region4 corresponding to amino acids 807 to 846 of SEQ ID NO:6, region 5corresponding to amino acids 872 to 885 of SEQ ID NO:6, and region 6corresponding to amino acids 903 to 1004 of SEQ ID NO:6. This relates toan important guidance on where to mutate a xanthan lyase in order tostabilize the molecule in a detergent, e.g. detergent compositioncomprising a chelator, e.g. EDTA or citrate.

In one embodiment, the detergent composition comprises a xanthan lyasevariant, comprising an alteration (e.g. a substitution, deletion orinsertion, preferably a substitution) at one or more positions in aregion selected from the group consisting of: region 1 corresponding toamino acids 154 to 176 of SEQ ID NO:6, region 2 corresponding to aminoacids 614 to 658 of SEQ ID NO:6, region 3 corresponding to amino acids731 to 803 of SEQ ID NO:6, region 4 corresponding to amino acids 807 to846 of SEQ ID NO:6, region 5 corresponding to amino acids 872 to 885 ofSEQ ID NO:6, and region 6 corresponding to amino acids 903 to 1004 ofSEQ ID NO:6, wherein said variant has at least 60% and less than 100%sequence identity to SEQ ID NO:6; preferably said xanthan lyase varianthas activity on xanthan gum, further preferably said activity is axanthan gum degrading activity.

In one embodiment, the detergent composition comprises a xanthan lyasevariant, comprising an alteration (e.g. a substitution, deletion orinsertion, preferably a substitution) at one or more positions in two ormore regions selected from the group consisting of: region 1corresponding to amino acids 154 to 176 of SEQ ID NO:6, region 2corresponding to amino acids 614 to 658 of SEQ ID NO:6, region 3corresponding to amino acids 731 to 803 of SEQ ID NO:6, region 4corresponding to amino acids 807 to 846 of SEQ ID NO:6, region 5corresponding to amino acids 872 to 885 of SEQ ID NO:6, and region 6corresponding to amino acids 903 to 1004 of SEQ ID NO:6, wherein saidvariant has at least 60% and less than 100% sequence identity to SEQ IDNO:6; preferably said xanthan lyase variant has activity on xanthan gum,further preferably said activity is a xanthan gum degrading activity.

In one embodiment, the detergent composition comprises a parent xanthanlyase as described herein (e.g. SEQ ID NO:6) having an alteration (e.g.a substitution, deletion or insertion, preferably a substitution) at oneor more positions in a region selected from the group consisting ofregions 1-6, wherein said region is a chelator-induced instabilityregion, preferably said chelator-induced instability region has one ormore of the following features: in the presence of a chelator it is lessconformationally stable than one or more or all of its adjacent regions;and/or in the presence of a chelator it is more exposed to said chelatorthan one or more or all of its adjacent regions; and/or in the presenceof a chelator it is more accessible to said chelator than one or more orall of its adjacent regions; and/or in the presence of a chelator it ismore conformationally dynamic than one or more or all of its adjacentregions; and/or in the presence of a chelator it is more receptive todeuterium incorporation than one or more or all of its adjacent regions;further preferably said adjacent region is selected from the groupconsisting of: region 7 corresponding to amino acids 1 to 153 of SEQ IDNO:6, region 8 corresponding to amino acids 177 to 613 of SEQ ID NO:6,region 9 corresponding to amino acids 659 to 730 of SEQ ID NO:6, region10 corresponding to amino acids 804 to 806 of SEQ ID NO:6, region 11corresponding to amino acids 847 to 871 of SEQ ID NO:6, region 12corresponding to amino acids 886 to 902 of SEQ ID NO:6, and region 13corresponding to amino acids 1005 to 1037 of SEQ ID NO:6, further mostpreferably said chelator is EDTA or citrate.

In one embodiment the adjacent regions can be one or more or all of thefollowing: region 7 corresponding to amino acids 1 to 153 of SEQ IDNO:6, region 8 corresponding to amino acids 177 to 613 of SEQ ID NO:6,region 9 corresponding to amino acids 659 to 730 of SEQ ID NO:6, region10 corresponding to amino acids 804 to 806 of SEQ ID NO:6, region 11corresponding to amino acids 847 to 871 of SEQ ID NO:6, region 12corresponding to amino acids 886 to 902 of SEQ ID NO:6, and region 13corresponding to amino acids 1005 to 1037 of SEQ ID NO:6.

In one embodiment, the detergent composition comprises a xanthan lyasevariant having an alteration (e.g. a substitution, deletion orinsertion, preferably a substitution) at one or more positions in aregion selected from the group consisting of regions 1-6 (e.g. of SEQ IDNO:6 or another parent xanthan lyase), wherein in an aqueous solutioncomprising a detergent component said region (e.g. of SEQ ID NO:6 oranother parent xanthan lyase) is relatively more accessible to saiddetergent component than one or more or all of its adjacent regions.

In one embodiment, the detergent composition comprises a xanthan lyasevariant having an alteration (e.g. a substitution, deletion orinsertion, preferably a substitution) at one or more positions in aregion selected from the group consisting of regions 1-6 (e.g. of SEQ IDNO:6 or another parent xanthan lyase), wherein in an aqueous solutioncomprising a detergent component said region (e.g. of SEQ ID NO:6 oranother parent xanthan lyase) is relatively more exposed to saiddetergent component than one or more or all of its adjacent regions.

In one embodiment, the detergent composition comprises a xanthan lyasevariant having an alteration (e.g. a substitution, deletion orinsertion, preferably a substitution) at one or more positions in aregion selected from the group consisting of regions 1-6 (e.g. of SEQ IDNO:6 or another parent xanthan lyase), wherein in an aqueous solutioncomprising a detergent component said region (e.g. of SEQ ID NO:6 oranother parent xanthan lyase) is relatively more conformationallydynamic than one or more or all of its adjacent regions.

In one embodiment, the detergent composition comprises a xanthan lyasevariant having an alteration (e.g. a substitution, deletion orinsertion, preferably a substitution) at one or more positions in aregion selected from the group consisting of regions 1-6 (e.g. of SEQ IDNO:6 or another parent xanthan lyase), wherein in an aqueous solutioncomprising a detergent component said region (e.g. of SEQ ID NO:6 oranother parent xanthan lyase) is relatively more receptive to deuteriumincorporation than one or more or all of its adjacent regions.

In one embodiment, the detergent composition comprises a xanthan lyasevariant of the invention, comprising an alteration (e.g. a substitution,deletion or insertion, preferably a substitution) at one or morepositions in two or more regions selected from the group consisting of:region 1 corresponding to amino acids 154 to 176 of SEQ ID NO:6, region2 corresponding to amino acids 614 to 658 of SEQ ID NO:6, region 3corresponding to amino acids 731 to 803 of SEQ ID NO:6, region 4corresponding to amino acids 807 to 846 of SEQ ID NO:6, region 5corresponding to amino acids 872 to 885 of SEQ ID NO:6, and region 6corresponding to amino acids 903 to 1004 of SEQ ID NO:6, wherein saidvariant has at least 60% and less than 100% sequence identity to SEQ IDNO:6, preferably said variant has activity on xanthan gum, furtherpreferably said activity is a xanthan gum degrading activity.

In one embodiment, the detergent composition comprises a xanthan lyasevariant having multiple alterations (such as 2, 3, 4, 5, 6, 7, 8, 9 or10) in one region (e.g. of SEQ ID NO:6 or another parent xanthan lyase)selected from the group consisting of: region 1 corresponding to aminoacids 154 to 176 of SEQ ID NO:6, region 2 corresponding to amino acids614 to 658 of SEQ ID NO:6, region 3 corresponding to amino acids 731 to803 of SEQ ID NO:6, region 4 corresponding to amino acids 807 to 846 ofSEQ ID NO:6, region 5 corresponding to amino acids 872 to 885 of SEQ IDNO:6, and region 6 corresponding to amino acids 903 to 1004 of SEQ IDNO:6, wherein said variant has at least 60% and less than 100% sequenceidentity to SEQ ID NO:6, preferably said variant has activity on xanthangum, further preferably said activity is a xanthan gum degradingactivity.

In one embodiment, the detergent composition comprises a xanthan lyasevariant having multiple alterations (e.g. 2, 3, 4, 5, 6, 7, 8, 9 or 10)in multiple regions (e.g. 2, 3, 4, 5, 6, 7, 8, 9 or 10) (e.g. of SEQ IDNO:6 or another parent xanthan lyase) selected from the group consistingof: region 1 corresponding to amino acids 154 to 176 of SEQ ID NO:6,region 2 corresponding to amino acids 614 to 658 of SEQ ID NO:6, region3 corresponding to amino acids 731 to 803 of SEQ ID NO:6, region 4corresponding to amino acids 807 to 846 of SEQ ID NO:6, region 5corresponding to amino acids 872 to 885 of SEQ ID NO:6, and region 6corresponding to amino acids 903 to 1004 of SEQ ID NO:6, wherein saidvariant has at least 60% and less than 100% sequence identity to SEQ IDNO:6, preferably said variant has activity on xanthan gum, furtherpreferably said activity is a xanthan gum degrading activity.

In one embodiment, the regions in the protein sequence of the knownxanthan lyase having SEQ ID NO:6 that have an impact on stability of themolecule, e.g. during storage in a liquid detergent composition, are thefollowing: region 7 corresponding to amino acids 1 to 153 of SEQ IDNO:6, region 8 corresponding to amino acids 177 to 613 of SEQ ID NO:6,region 9 corresponding to amino acids 659 to 730 of SEQ ID NO:6, region10 corresponding to amino acids 804 to 806 of SEQ ID NO:6, region 11corresponding to amino acids 847 to 871 of SEQ ID NO:6, region 12corresponding to amino acids 886 to 902 of SEQ ID NO:6, and region 13corresponding to amino acids 1005 to 1037 of SEQ ID NO:6. Thisembodiment relates to an important guidance on where to mutate a xanthanlyase in order to stabilize the molecule in a detergent.

In one embodiment, the detergent compositions comprises a xanthan lyasevariant, comprising an alteration (e.g. a substitution, deletion orinsertion, preferably a substitution) at one or more positions in aregion selected from the group consisting of: region 7 corresponding toamino acids 1 to 153 of SEQ ID NO:6, region 8 corresponding to aminoacids 177 to 613 of SEQ ID NO:6, region 9 corresponding to amino acids659 to 730 of SEQ ID NO:6, region 10 corresponding to amino acids 804 to806 of SEQ ID NO:6, region 11 corresponding to amino acids 847 to 871 ofSEQ ID NO:6, region 12 corresponding to amino acids 886 to 902 of SEQ IDNO:6, and region 13 corresponding to amino acids 1005 to 1037 of SEQ IDNO:6, wherein said variant has at least 60% and less than 100% sequenceidentity to SEQ ID NO:6; preferably said xanthan lyase variant hasactivity on xanthan gum, further preferably said activity is a xanthangum degrading activity.

In one embodiment, the detergent compositions comprise a xanthan lyasevariant, comprising an alteration (e.g. a substitution, deletion orinsertion, preferably a substitution) at one or more positions in aregion selected from the group consisting of: (i) region 7 correspondingto amino acids 1 to 153 of SEQ ID NO:6; (ii) region 8 corresponding toamino acids 177 to 613 of SEQ ID NO:6; (iii) region 9 corresponding toamino acids 659 to 730 of SEQ ID NO:6; (iv) region 10 corresponding toamino acids 804 to 806 of SEQ ID NO:6; (v) region 11 corresponding toamino acids 847 to 871 of SEQ ID NO:6; (vi) region 12 corresponding toamino acids 886 to 902 of SEQ ID NO:6; (vii) region 13 corresponding toamino acids 1005 to 1037 of SEQ ID NO:6.

In one embodiment, the detergent compositions comprise a xanthan lyasevariant, comprising an alteration (e.g. a substitution, deletion orinsertion, preferably a substitution) at one or more positions in two ormore regions selected from the group consisting of: region 7corresponding to amino acids 1 to 153 of SEQ ID NO:6, region 8corresponding to amino acids 177 to 613 of SEQ ID NO:6, region 9corresponding to amino acids 659 to 730 of SEQ ID NO:6, region 10corresponding to amino acids 804 to 806 of SEQ ID NO:6, region 11corresponding to amino acids 847 to 871 of SEQ ID NO:6, region 12corresponding to amino acids 886 to 902 of SEQ ID NO:6, and region 13corresponding to amino acids 1005 to 1037 of SEQ ID NO:6, wherein saidvariant has at least 60% and less than 100% sequence identity to SEQ IDNO:6; preferably said xanthan lyase variant has activity on xanthan gum,further preferably said activity is a xanthan gum degrading activity.

In one embodiment, the detergent compositions comprise a xanthan lyasevariant as described herein having multiple alterations (such as 2, 3,4, 5, 6, 7, 8, 9 or 10) in one region (e.g. of SEQ ID NO:6 or anotherparent xanthan lyase) selected from the group consisting of: region 7corresponding to amino acids 1 to 153 of SEQ ID NO:6, region 8corresponding to amino acids 177 to 613 of SEQ ID NO:6, region 9corresponding to amino acids 659 to 730 of SEQ ID NO:6, region 10corresponding to amino acids 804 to 806 of SEQ ID NO:6, region 11corresponding to amino acids 847 to 871 of SEQ ID NO:6, region 12corresponding to amino acids 886 to 902 of SEQ ID NO:6, and region 13corresponding to amino acids 1005 to 1037 of SEQ ID NO:6, wherein saidvariant has at least 60% and less than 100% sequence identity to SEQ IDNO:6, preferably said variant has activity on xanthan gum, furtherpreferably said activity is a xanthan gum degrading activity.

In one embodiment, the detergent compositions comprise a xanthan lyasevariant as described herein having multiple alterations (e.g. 2, 3, 4,5, 6, 7, 8, 9 or 10) in multiple regions (e.g. 2, 3, 4, 5, 6 or 7) (e.g.of SEQ ID NO:6 or another parent xanthan lyase) selected from the groupconsisting of: region 7 corresponding to amino acids 1 to 153 of SEQ IDNO:6, region 8 corresponding to amino acids 177 to 613 of SEQ ID NO:6,region 9 corresponding to amino acids 659 to 730 of SEQ ID NO:6, region10 corresponding to amino acids 804 to 806 of SEQ ID NO:6, region 11corresponding to amino acids 847 to 871 of SEQ ID NO:6, region 12corresponding to amino acids 886 to 902 of SEQ ID NO:6, and region 13corresponding to amino acids 1005 to 1037 of SEQ ID NO:6, wherein saidvariant has at least 60% and less than 100% sequence identity to SEQ IDNO:6, preferably said variant has activity on xanthan gum, furtherpreferably said activity is a xanthan gum degrading activity.

In one embodiment, the detergent composition comprises xanthan lyasevariants, comprising an alteration (e.g. a substitution, deletion orinsertion, preferably a substitution) at one or more positions of themature parent polypeptide (e.g. SEQ ID NO:6), wherein each alteration isindependently a substitution, insertion or deletion, wherein the varianthas xanthan lyase activity.

In an embodiment, the variant has sequence identity of at least 60%,e.g. at least 65%, 70%, 75%, 80%, 85%, 90%, 91%, 92%, 93%, 94%, 95%,96%, 97%, 98%, or 99%, but less than 100%, to the amino acid sequence ofthe parent xanthan lyase.

In one embodiment, the variant has at least 60%, e.g. at least 65%, 70%,75%, 80%, 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, or 99%, butless than 100%, sequence identity to the mature polypeptide of SEQ IDNO:6.

In one embodiment, the detergent composition comprises a xanthan lyasevariant as described herein, having at least 61%, 62%, 63%, 64%, 65%,66%, 67%, 68%, 69%, 70%, 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%,80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%,94%, 95%, 96%, 97%, 98%, or 99% sequence identity to SEQ ID NO:6.

In another aspect, a variant comprises an alteration at one or morepositions corresponding to positions 155, 159, 620, 624, 626, 631, 635,645, 649, 650, 656, 738, 745, 746, 748, 752, 753, 754, 757, 764, 769,774, 775, 777, 779, 782, 785, 786, 789, 792, 796, 799, 800, 801, 819,824, 843, 845, 875, 903, 911, 912, 915, 919, 921, 923, 925, 927, 928,930, 932, 933, 941, 966, 967, 991 and 998. In another aspect, a variantcomprises an alteration at two positions corresponding to any ofpositions 155, 159, 620, 624, 626, 631, 635, 645, 649, 650, 656, 738,745, 746, 748, 752, 753, 754, 757, 764, 769, 774, 775, 777, 779, 782,785, 786, 789, 792, 796, 799, 800, 801, 819, 824, 843, 845, 875, 903,911, 912, 915, 919, 921, 923, 925, 927, 928, 930, 932, 933, 941, 966,967, 991 and 998. In another aspect, a variant comprises an alterationat three positions corresponding to any of positions 155, 159, 620, 624,626, 631, 635, 645, 649, 650, 656, 738, 745, 746, 748, 752, 753, 754,757, 764, 769, 774, 775, 777, 779, 782, 785, 786, 789, 792, 796, 799,800, 801, 819, 824, 843, 845, 875, 903, 911, 912, 915, 919, 921, 923,925, 927, 928, 930, 932, 933, 941, 966, 967, 991 and 998. In anotheraspect, a variant comprises an alteration at four or more positions,e.g. five, six, seven, eight, nine, ten or more positions, correspondingto positions 155, 159, 620, 624, 626, 631, 635, 645, 649, 650, 656, 738,745, 746, 748, 752, 753, 754, 757, 764, 769, 774, 775, 777, 779, 782,785, 786, 789, 792, 796, 799, 800, 801, 819, 824, 843, 845, 875, 903,911, 912, 915, 919, 921, 923, 925, 927, 928, 930, 932, 933, 941, 966,967, 991 and 998.

In one embodiment, the detergent composition comprises a xanthan lyasevariant, having an alteration at one or more positions selected from thegroup consisting of positions: 155, 159, 620, 624, 626, 631, 635, 645,649, 650, 656, 738, 745, 746, 748, 752, 753, 754, 757, 764, 769, 774,775, 777, 779, 782, 785, 786, 789, 792, 796, 799, 800, 801, 819, 824,843, 845, 875, 903, 911, 912, 915, 919, 921, 923, 925, 927, 928, 930,932, 933, 941, 966, 967, 991 and 998 of SEQ ID NO:6, wherein eachposition corresponds to the positions of SEQ ID NO:6.

In another aspect, a variant comprises an alteration at one or morepositions corresponding to positions 9, 15, 46, 58, 66, 89, 95, 100,106, 109, 183, 188, 190, 203, 204, 221, 229, 234, 238, 240, 242, 243,257, 258, 291, 293, 316, 320, 324, 329, 333, 339, 341, 352, 354, 360,377, 400, 419, 450, 451, 454, 481, 492, 567, 568, 578, 579, 664, 672,855, 887 and 892. In another aspect, a variant comprises an alterationat two positions corresponding to any of positions 9, 15, 46, 58, 66,89, 95, 100, 106, 109, 183, 188, 190, 203, 204, 221, 229, 234, 238, 240,242, 243, 257, 258, 291, 293, 316, 320, 324, 329, 333, 339, 341, 352,354, 360, 377, 400, 419, 450, 451, 454, 481, 492, 567, 568, 578, 579,664, 672, 855, 887 and 892. In another aspect, a variant comprises analteration at three positions corresponding to any of positions 9, 15,46, 58, 66, 89, 95, 100, 106, 109, 183, 188, 190, 203, 204, 221, 229,234, 238, 240, 242, 243, 257, 258, 291, 293, 316, 320, 324, 329, 333,339, 341, 352, 354, 360, 377, 400, 419, 450, 451, 454, 481, 492, 567,568, 578, 579, 664, 672, 855, 887 and 892. In another aspect, a variantcomprises an alteration at four or more positions, e.g. five, six,seven, eight, nine, ten or more positions, corresponding to positions 9,15, 46, 58, 66, 89, 95, 100, 106, 109, 183, 188, 190, 203, 204, 221,229, 234, 238, 240, 242, 243, 257, 258, 291, 293, 316, 320, 324, 329,333, 339, 341, 352, 354, 360, 377, 400, 419, 450, 451, 454, 481, 492,567, 568, 578, 579, 664, 672, 855, 887 and 892.

In one embodiment, the detergent composition comprises a xanthan lyasevariant, having an alteration at one or more positions selected from thegroup consisting of: Y155E, A159P, K620R, A624E, A626G, T631N, T631E,S635E, S635T, S635Q, A645S, T649V, T649K, T649R, Q650G, I656V, G738L,K745R, F746L, L748T, P752R, P752K, G753E, G753Q, G753S, S754E, S754L,S754Q, S754R, S757D, S757P, S757E, P764V, P764K, A769D, A769T, A769R,A769S, A769E, A769Q, A769*, A774V, L775M, L775Y, L775A, L7751, L7755,L775F, L775Q, D777K, D777R, P779V, Y7821, A785T, N786K, G789R, K792W,K792Y, K792V, K792A, N796Q, A799H, V800P, D801G, K819R, K819T, K824R,A843P, D845E, K875T, K875E, T903A, T903Q, A911V, A911M, A911S, A912T,A9121, A912Y, T915Q, T915S, T915V, T915A, T919F, T919G, T919D, T921R,T921S, T923H, T923D, T925Q, T925D, T925R, T927K, D928W, Y930H, Y930L,Y930F, A932P, D933M, G941E, G941D, A966P, A967D, N991D and V998K.,wherein numbering is according to SEQ ID NO:6.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 155. In another aspect, the aminoacid at a position corresponding to position 155 is substituted withAla, Arg, Asn, Asp, Cys, Gin, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution Y155E of the maturepolypeptide of SEQ ID NO:6.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 159. In another aspect, the aminoacid at a position corresponding to position 159 is substituted withAla, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution A159P.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 620. In another aspect, the aminoacid at a position corresponding to position 620 is substituted withAla, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution K620R of the maturepolypeptide of SEQ ID NO:6.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 624. In another aspect, the aminoacid at a position corresponding to position 624 is substituted withAla, Arg, Asn, Asp, Cys, Gin, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution A624E of the maturepolypeptide of SEQ ID NO:6

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 626. In another aspect, the aminoacid at a position corresponding to position 626 is substituted withAla, Arg, Asn, Asp, Cys, Gin, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution A626Q of the maturepolypeptide of SEQ ID NO:6.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 631. In another aspect, the aminoacid at a position corresponding to position 631 is substituted withAla, Arg, Asn, Asp, Cys, Gin, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution T631N or T631E of the maturepolypeptide of SEQ ID NO:6. A preferred substitution at a positioncorresponding to position 631 is T631N.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 635. In another aspect, the aminoacid at a position corresponding to position 635 is substituted withAla, Arg, Asn, Asp, Cys, Gin, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution S635E, S635T or S635Q. Apreferred substitution at a position corresponding to position 635 isS635E.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 649. In another aspect, the aminoacid at a position corresponding to position 649 is substituted withAla, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution T649V, T649K or T649R of themature polypeptide of SEQ ID NO:6. A preferred substitution at aposition corresponding to position 649 is T649K.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 650. In another aspect, the aminoacid at a position corresponding to position 650 is substituted withAla, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution Q650G of the maturepolypeptide of SEQ ID NO:6.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 656. In another aspect, the aminoacid at a position corresponding to position 656 is substituted withAla, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution I656V of the maturepolypeptide of SEQ ID NO:6.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 738. In another aspect, the aminoacid at a position corresponding to position 738 is substituted withAla, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution G738L of the maturepolypeptide of SEQ ID NO:6.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 745. In another aspect, the aminoacid at a position corresponding to position 745 is substituted withAla, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution K745R of the maturepolypeptide of SEQ ID NO:6.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 746. In another aspect, the aminoacid at a position corresponding to position 746 is substituted withAla, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution F746L of the maturepolypeptide of SEQ ID NO:6.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 748. In another aspect, the aminoacid at a position corresponding to position 748 is substituted withAla, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution L748T of the maturepolypeptide of SEQ ID NO:6.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 752. In another aspect, the aminoacid at a position corresponding to position 752 is substituted withAla, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution P752R or P752K of the maturepolypeptide of SEQ ID NO:6.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 753. In another aspect, the aminoacid at a position corresponding to position 753 is substituted withAla, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution G753E, G753Q or G753S of themature polypeptide of SEQ ID NO:6. A preferred substitution at aposition corresponding to position 753 is G753E.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 754. In another aspect, the aminoacid at a position corresponding to position 754 is substituted withAla, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution S754E, S754L, S754Q or S754Rof the mature polypeptide of SEQ ID NO:6. A preferred substitution at aposition corresponding to position 754 is S754E or S754R.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 757. In another aspect, the aminoacid at a position corresponding to position 757 is substituted withAla, Arg, Asn, Asp, Cys, Gin, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution S757D, S757P or S757E of themature polypeptide of SEQ ID NO:6. A preferred substitution at aposition corresponding to position 757 is S757D.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 764. In another aspect, the aminoacid at a position corresponding to position 764 is substituted withAla, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution P764V or P764K of the maturepolypeptide of SEQ ID NO:6. A preferred substitution at a positioncorresponding to position 764 is P764V.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 769. In another aspect, the aminoacid at a position corresponding to position 769 is substituted withAla, Arg, Asn, Asp, Cys, Gin, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the alteration A769D, A769T, A769R, A769S,A769E, A769Q or A769* of the mature polypeptide of SEQ ID NO:6. Apreferred substitution at a position corresponding to position 769 isA769D.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 774. In another aspect, the aminoacid at a position corresponding to position 774 is substituted withAla, Arg, Asn, Asp, Cys, Gin, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution A774V of the maturepolypeptide of SEQ ID NO:6.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 775. In another aspect, the aminoacid at a position corresponding to position 775 is substituted withAla, Arg, Asn, Asp, Cys, Gin, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution L775A or L775F or L775I orL775M or L775Q or L7755 or L775Y of the mature polypeptide of SEQ IDNO:6. A preferred substitution at a position corresponding to position775 is L775M, L775Y or L775A.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 779. In another aspect, the aminoacid at a position corresponding to position 779 is substituted withAla, Arg, Asn, Asp, Cys, Gin, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution P779V of the maturepolypeptide of SEQ ID NO:6.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 782. In another aspect, the aminoacid at a position corresponding to position 782 is substituted withAla, Arg, Asn, Asp, Cys, Gin, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution Y782I.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 786. In another aspect, the aminoacid at a position corresponding to position 786 is substituted withAla, Arg, Asn, Asp, Cys, Gin, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution N786K.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 789. In another aspect, the aminoacid at a position corresponding to position 789 is substituted withAla, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution G789R.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 792. In another aspect, the aminoacid at a position corresponding to position 792 is substituted withAla, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution K792W, K792Y, K792V or K792Aof the mature polypeptide of SEQ ID NO:6. A preferred substitution at aposition corresponding to position 792 is K792W or K792Y.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 796. In another aspect, the aminoacid at a position corresponding to position 796 is substituted withAla, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution N796Q of the maturepolypeptide of SEQ ID NO:6.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 799. In another aspect, the aminoacid at a position corresponding to position 799 is substituted withAla, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution A799H of the maturepolypeptide of SEQ ID NO:6.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 800. In another aspect, the aminoacid at a position corresponding to position 800 is substituted withAla, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution V800P of the maturepolypeptide of SEQ ID NO:6.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 801. In another aspect, the aminoacid at a position corresponding to position 801 is substituted withAla, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution D801G of the maturepolypeptide of SEQ ID NO:6.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 819. In another aspect, the aminoacid at a position corresponding to position 819 is substituted withAla, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution K819R or K819T of the maturepolypeptide of SEQ ID NO:6. A preferred substitution at a positioncorresponding to position 819 is K819R or K819T.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 824. In another aspect, the aminoacid at a position corresponding to position 824 is substituted withAla, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution K824R of the maturepolypeptide of SEQ ID NO:6.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 843. In another aspect, the aminoacid at a position corresponding to position 843 is substituted withAla, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution A843P.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 845. In another aspect, the aminoacid at a position corresponding to position 845 is substituted withAla, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. A preferred substitution at a positioncorresponding to position 845 is D845E.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 875. In another aspect, the aminoacid at a position corresponding to position 875 is substituted withAla, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution K875T or K875E of the maturepolypeptide of SEQ ID NO:6. A preferred substitution at a positioncorresponding to position 875 is K875T.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 903. In another aspect, the aminoacid at a position corresponding to position 903 is substituted withAla, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution T903A or T903Q of the maturepolypeptide of SEQ ID NO:6.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 911. In another aspect, the aminoacid at a position corresponding to position 911 is substituted withAla, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution A911M, A911V or A911S of themature polypeptide of SEQ ID NO:6. A preferred substitution at aposition corresponding to position 911 is A911V.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 912. In another aspect, the aminoacid at a position corresponding to position 912 is substituted withAla, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution A912I or A912T or A912Y of themature polypeptide of SEQ ID NO:6. A preferred substitution at aposition corresponding to position 912 is A912T.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 915. In another aspect, the aminoacid at a position corresponding to position 915 is substituted withAla, Arg, Asn, Asp, Cys, Gin, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution T915S, T915Q, T915A or T915Vof the mature polypeptide of SEQ ID NO:6.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 919. In another aspect, the aminoacid at a position corresponding to position 919 is substituted withAla, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution T919D, T919F or T919G of themature polypeptide of SEQ ID NO:6.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 921. In another aspect, the aminoacid ata position corresponding to position 921 is substituted with Ala,Arg, Asn, Asp, Cys, Gin, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro,Ser, Thr, Trp, Tyr, or Val. In another aspect, the variant comprises orconsists of the substitution T921R or T921S of the mature polypeptide ofSEQ ID NO:6.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 923. In another aspect, the aminoacid at a position corresponding to position 923 is substituted withAla, Arg, Asn, Asp, Cys, Gin, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution T923D or T923H of the maturepolypeptide of SEQ ID NO:6.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 925. In another aspect, the aminoacid at a position corresponding to position 925 is substituted withAla, Arg, Asn, Asp, Cys, Gin, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution T925D or T925Q or T925R of themature polypeptide of SEQ ID NO:6.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 927. In another aspect, the aminoacid at a position corresponding to position 927 is substituted withAla, Arg, Asn, Asp, Cys, Gin, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution T927K of the maturepolypeptide of SEQ ID NO:6.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 928. In another aspect, the aminoacid at a position corresponding to position 928 is substituted withAla, Arg, Asn, Asp, Cys, Gin, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution D928W of the maturepolypeptide of SEQ ID NO:6.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 930. In another aspect, the aminoacid at a position corresponding to position 930 is substituted withAla, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution Y930F or Y930H or Y930L of themature polypeptide of SEQ ID NO:6.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 933. In another aspect, the aminoacid at a position corresponding to position 933 is substituted withAla, Arg, Asn, Asp, Cys, Gin, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution D933M of the maturepolypeptide of SEQ ID NO:6.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 941. In another aspect, the aminoacid at a position corresponding to position 941 is substituted withAla, Arg, Asn, Asp, Cys, Gin, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution G941D or G941E of the maturepolypeptide of SEQ ID NO:6.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 966. In another aspect, the aminoacid at a position corresponding to position 966 is substituted withAla, Arg, Asn, Asp, Cys, Gin, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution A966P of the maturepolypeptide of SEQ ID NO:6.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 991. In another aspect, the aminoacid at a position corresponding to position 991 is substituted withAla, Arg, Asn, Asp, Cys, Gin, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution N991D of the maturepolypeptide of SEQ ID NO:6.

In another aspect, the variant comprises or consists of an alteration ata position corresponding to position 998. In another aspect, the aminoacid at a position corresponding to position 998 is substituted withAla, Arg, Asn, Asp, Cys, Gin, Glu, Gly, His, Ile, Leu, Lys, Met, Phe,Pro, Ser, Thr, Trp, Tyr, or Val. In another aspect, the variantcomprises or consists of the substitution V998K of the maturepolypeptide of SEQ ID NO:6.

In one aspect, the variant comprises or consists of an alteration at aposition corresponding to position 9. In one embodiment, the amino acidat this position may substituted with Ala, Arg, Asn, Asp, Cys, Gin, Glu,Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val. In aparticular embodiment, the variant comprises or consists of thesubstitution K9R of the mature polypeptide of SEQ ID NO:6.

In one aspect, the variant comprises or consists of an alteration at aposition corresponding to position 15. In one embodiment, the amino acidat this position may substituted with Ala, Arg, Asn, Asp, Cys, Gin, Glu,Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val. In aparticular embodiment, the variant comprises or consists of thesubstitution N15T of the mature polypeptide of SEQ ID NO:6.

In one aspect, the variant comprises or consists of an alteration at aposition corresponding to position 46. In one embodiment, the amino acidat this position may substituted with Ala, Arg, Asn, Asp, Cys, Gin, Glu,Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val. In aparticular embodiment, the variant comprises or consists of thesubstitution L46D of the mature polypeptide of SEQ ID NO:6.

In one aspect, the variant comprises or consists of an alteration at aposition corresponding to position 58. In one embodiment, the amino acidat this position may substituted with Ala, Arg, Asn, Asp, Cys, Gln, Glu,Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val. In aparticular embodiment, the variant comprises or consists of thesubstitution A58L of the mature polypeptide of SEQ ID NO:6.

In one aspect, the variant comprises or consists of an alteration at aposition corresponding to position 66. In one embodiment, the amino acidat this position may substituted with Ala, Arg, Asn, Asp, Cys, Gin, Glu,Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val. In aparticular embodiment, the variant comprises or consists of thesubstitution S66H of the mature polypeptide of SEQ ID NO:6.

In one aspect, the variant comprises or consists of an alteration at aposition corresponding to position 89. In one embodiment, the amino acidat this position may substituted with Ala, Arg, Asn, Asp, Cys, Gin, Glu,Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val. In aparticular embodiment, the variant comprises or consists of thesubstitution Q89Y of the mature polypeptide of SEQ ID NO:6.

In one aspect, the variant comprises or consists of an alteration at aposition corresponding to position 95. In one embodiment, the amino acidat this position may substituted with Ala, Arg, Asn, Asp, Cys, Gin, Glu,Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val. In aparticular embodiment, the variant comprises or consists of thesubstitution K95E of the mature polypeptide of SEQ ID NO:6.

In one aspect, the variant comprises or consists of an alteration at aposition corresponding to position 100. In one embodiment, the aminoacid at this position may substituted with Ala, Arg, Asn, Asp, Cys, Gin,Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val.In a particular embodiment, the variant comprises or consists of thesubstitution S100D of the mature polypeptide of SEQ ID NO:6.

In one aspect, the variant comprises or consists of an alteration at aposition corresponding to position 106. In one embodiment, the aminoacid at this position may substituted with Ala, Arg, Asn, Asp, Cys, Gin,Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val.In a particular embodiment, the variant comprises or consists of thesubstitution N106Y of the mature polypeptide of SEQ ID NO:6.

In one aspect, the variant comprises or consists of an alteration at aposition corresponding to position 109. In one embodiment, the aminoacid at this position may substituted with Ala, Arg, Asn, Asp, Cys, Gin,Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val.In a particular embodiment, the variant comprises or consists of thesubstitution Q109R, Q109D, Q109F, Q109K or Q109A of the maturepolypeptide of SEQ ID NO:6. A preferred substitution at a positioncorresponding to position 109 is Q109R.

In one aspect, the variant comprises or consists of an alteration at aposition corresponding to position 183. In one embodiment, the aminoacid at this position may substituted with Ala, Arg, Asn, Asp, Cys, Gln,Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val.In a particular embodiment, the variant comprises or consists of thesubstitution K183Q or K183R of the mature polypeptide of SEQ ID NO:6.

In one aspect, the variant comprises or consists of an alteration at aposition corresponding to position 188. In one embodiment, the aminoacid at this position may substituted with Ala, Arg, Asn, Asp, Cys, Gln,Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val.In a particular embodiment, the variant comprises or consists of thesubstitution V1881 of the mature polypeptide of SEQ ID NO:6.

In one aspect, the variant comprises or consists of an alteration at aposition corresponding to position 190. In one embodiment, the aminoacid at this position may substituted with Ala, Arg, Asn, Asp, Cys, Gin,Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val.In a particular embodiment, the variant comprises or consists of thesubstitution Al 90Q of the mature polypeptide of SEQ ID NO:6.

In one aspect, the variant comprises or consists of an alteration at aposition corresponding to position 203. In one embodiment, the aminoacid at this position may substituted with Ala, Arg, Asn, Asp, Cys, Gin,Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val.In a particular embodiment, the variant comprises or consists of thesubstitution A203P of the mature polypeptide of SEQ ID NO:6.

In one aspect, the variant comprises or consists of an alteration at aposition corresponding to position 204. In one embodiment, the aminoacid at this position may substituted with Ala, Arg, Asn, Asp, Cys, Gin,Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val.In a particular embodiment, the variant comprises or consists of thesubstitution K204R of the mature polypeptide of SEQ ID NO:6.

In one aspect, the variant comprises or consists of an alteration at aposition corresponding to position 221. In one embodiment, the aminoacid at this position may substituted with Ala, Arg, Asn, Asp, Cys, Gin,Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val.In a particular embodiment, the variant comprises or consists of thesubstitution A221P of the mature polypeptide of SEQ ID NO:6.

In one aspect, the variant comprises or consists of an alteration at aposition corresponding to position 229. In one embodiment, the aminoacid at this position may substituted with Ala, Arg, Asn, Asp, Cys, Gin,Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val.In a particular embodiment, the variant comprises or consists of thesubstitution E229N or E229S of the mature polypeptide of SEQ ID NO:6.

In one aspect, the variant comprises or consists of an alteration at aposition corresponding to position 234. In one embodiment, the aminoacid at this position may substituted with Ala, Arg, Asn, Asp, Cys, Gln,Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val.In a particular embodiment, the variant comprises or consists of thesubstitution I234V of the mature polypeptide of SEQ ID NO:6.

In one aspect, the variant comprises or consists of an alteration at aposition corresponding to position 238. In one embodiment, the aminoacid at this position may substituted with Ala, Arg, Asn, Asp, Cys, Gln,Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val.In a particular embodiment, the variant comprises or consists of thesubstitution I238W, I238L or I238M of the mature polypeptide of SEQ IDNO:6. Preferred substitutions at a position corresponding to position238 are I238W and I238L.

In one aspect, the variant comprises or consists of an alteration at aposition corresponding to position 240. In one embodiment, the aminoacid at this position may substituted with Ala, Arg, Asn, Asp, Cys, Gln,Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val.In a particular embodiment, the variant comprises or consists of thesubstitution 1240W of the mature polypeptide of SEQ ID NO:6.

In one aspect, the variant comprises or consists of an alteration at aposition corresponding to position 242. In one embodiment, the aminoacid at this position may substituted with Ala, Arg, Asn, Asp, Cys, Gln,Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val.In a particular embodiment, the variant comprises or consists of thesubstitution N242S of the mature polypeptide of SEQ ID NO:6.

In one aspect, the variant comprises or consists of an alteration at aposition corresponding to position 243. In one embodiment, the aminoacid at this position may substituted with Ala, Arg, Asn, Asp, Cys, Gln,Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val.In a particular embodiment, the variant comprises or consists of thesubstitution G243V of the mature polypeptide of SEQ ID NO:6.

In one aspect, the variant comprises or consists of an alteration at aposition corresponding to position 257. In one embodiment, the aminoacid at this position may substituted with Ala, Arg, Asn, Asp, Cys, Gln,Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val.In a particular embodiment, the variant comprises or consists of thesubstitution Y257W of the mature polypeptide of SEQ ID NO:6.

In one aspect, the variant comprises or consists of an alteration at aposition corresponding to position 258. In one embodiment, the aminoacid at this position may substituted with Ala, Arg, Asn, Asp, Cys, Gln,Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val.In a particular embodiment, the variant comprises or consists of thesubstitution R258E of the mature polypeptide of SEQ ID NO:6.

In one aspect, the variant comprises or consists of an alteration at aposition corresponding to position 291. In one embodiment, the aminoacid at this position may substituted with Ala, Arg, Asn, Asp, Cys, Gln,Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val.In a particular embodiment, the variant comprises or consists of thesubstitution K291R of the mature polypeptide of SEQ ID NO:6.

In one aspect, the variant comprises or consists of an alteration at aposition corresponding to position 293. In one embodiment, the aminoacid at this position may substituted with Ala, Arg, Asn, Asp, Cys, Gin,Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val.In a particular embodiment, the variant comprises or consists of thesubstitution A293G or A293P of the mature polypeptide of SEQ ID NO:6.

In one aspect, the variant comprises or consists of an alteration at aposition corresponding to position 316. In one embodiment, the aminoacid at this position may substituted with Ala, Arg, Asn, Asp, Cys, Gln,Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val.In a particular embodiment, the variant comprises or consists of thesubstitution K316R of the mature polypeptide of SEQ ID NO:6.

In one aspect, the variant comprises or consists of an alteration at aposition corresponding to position 320. In one embodiment, the aminoacid at this position may substituted with Ala, Arg, Asn, Asp, Cys, Gin,Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val.In a particular embodiment, the variant comprises or consists of thesubstitution K320R of the mature polypeptide of SEQ ID NO:6.

In one aspect, the variant comprises or consists of an alteration at aposition corresponding to position 324. In one embodiment, the aminoacid at this position may substituted with Ala, Arg, Asn, Asp, Cys, Gin,Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val.In a particular embodiment, the variant comprises or consists of thesubstitution L324Q of the mature polypeptide of SEQ ID NO:6.

In one aspect, the variant comprises or consists of an alteration at aposition corresponding to position 329. In one embodiment, the aminoacid at this position may substituted with Ala, Arg, Asn, Asp, Cys, Gin,Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val.In a particular embodiment, the variant comprises or consists of thesubstitution K329R of the mature polypeptide of SEQ ID NO:6.

In one aspect, the variant comprises or consists of an alteration at aposition corresponding to position 333. In one embodiment, the aminoacid at this position may substituted with Ala, Arg, Asn, Asp, Cys, Gin,Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val.In a particular embodiment, the variant comprises or consists of thesubstitution K333R of the mature polypeptide of SEQ ID NO:6.

In one aspect, the variant comprises or consists of an alteration at aposition corresponding to position 339. In one embodiment, the aminoacid at this position may substituted with Ala, Arg, Asn, Asp, Cys, Gin,Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val.In a particular embodiment, the variant comprises or consists of thesubstitution L339M of the mature polypeptide of SEQ ID NO:6.

In one aspect, the variant comprises or consists of an alteration at aposition corresponding to position 341. In one embodiment, the aminoacid at this position may substituted with Ala, Arg, Asn, Asp, Cys, Gin,Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val.In a particular embodiment, the variant comprises or consists of thesubstitution 1341P of the mature polypeptide of SEQ ID NO:6.

In one aspect, the variant comprises or consists of an alteration at aposition corresponding to position 352. In one embodiment, the aminoacid at this position may substituted with Ala, Arg, Asn, Asp, Cys, Gln,Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val.In a particular embodiment, the variant comprises or consists of thesubstitution V3521 of the mature polypeptide of SEQ ID NO:6.

In one aspect, the variant comprises or consists of an alteration at aposition corresponding to position 354. In one embodiment, the aminoacid at this position may substituted with Ala, Arg, Asn, Asp, Cys, Gln,Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val.In a particular embodiment, the variant comprises or consists of thesubstitution S354P of the mature polypeptide of SEQ ID NO:6.

In one aspect, the variant comprises or consists of an alteration at aposition corresponding to position 360. In one embodiment, the aminoacid at this position may substituted with Ala, Arg, Asn, Asp, Cys, Gin,Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val.In a particular embodiment, the variant comprises or consists of thesubstitution K360R of the mature polypeptide of SEQ ID NO:6.

In one aspect, the variant comprises or consists of an alteration at aposition corresponding to position 377. In one embodiment, the aminoacid at this position may substituted with Ala, Arg, Asn, Asp, Cys, Gin,Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val.In a particular embodiment, the variant comprises or consists of thesubstitution F377Y of the mature polypeptide of SEQ ID NO:6.

In one aspect, the variant comprises or consists of an alteration at aposition corresponding to position 400. In one embodiment, the aminoacid at this position may substituted with Ala, Arg, Asn, Asp, Cys, Gin,Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val.In a particular embodiment, the variant comprises or consists of thesubstitution K400R of the mature polypeptide of SEQ ID NO:6.

In one aspect, the variant comprises or consists of an alteration at aposition corresponding to position 419. In one embodiment, the aminoacid at this position may substituted with Ala, Arg, Asn, Asp, Cys, Gin,Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val.In a particular embodiment, the variant comprises or consists of thesubstitution F419Y of the mature polypeptide of SEQ ID NO:6.

In one aspect, the variant comprises or consists of an alteration at aposition corresponding to position 450. In one embodiment, the aminoacid at this position may substituted with Ala, Arg, Asn, Asp, Cys, Gin,Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val.In a particular embodiment, the variant comprises or consists of thesubstitution D450P of the mature polypeptide of SEQ ID NO:6.

In one aspect, the variant comprises or consists of an alteration at aposition corresponding to position 451. In one embodiment, the aminoacid at this position may substituted with Ala, Arg, Asn, Asp, Cys, Gin,Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val.In a particular embodiment, the variant comprises or consists of thesubstitution K451E or K451R of the mature polypeptide of SEQ ID NO:6.

In one aspect, the variant comprises or consists of an alteration at aposition corresponding to position 454. In one embodiment, the aminoacid at this position may substituted with Ala, Arg, Asn, Asp, Cys, Gln,Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val.In a particular embodiment, the variant comprises or consists of thesubstitution A454V of the mature polypeptide of SEQ ID NO:6.

In one aspect, the variant comprises or consists of an alteration at aposition corresponding to position 481. In one embodiment, the aminoacid at this position may substituted with Ala, Arg, Asn, Asp, Cys, Gln,Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val.In a particular embodiment, the variant comprises or consists of thesubstitution K481R of the mature polypeptide of SEQ ID NO:6.

In one aspect, the variant comprises or consists of an alteration at aposition corresponding to position 492. In one embodiment, the aminoacid at this position may substituted with Ala, Arg, Asn, Asp, Cys, Gln,Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val.In a particular embodiment, the variant comprises or consists of thesubstitution A492L of the mature polypeptide of SEQ ID NO:6.

In one aspect, the variant comprises or consists of an alteration at aposition corresponding to position 567. In one embodiment, the aminoacid at this position may substituted with Ala, Arg, Asn, Asp, Cys, Gln,Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val.In a particular embodiment, the variant comprises or consists of thesubstitution K567R of the mature polypeptide of SEQ ID NO:6.

In one aspect, the variant comprises or consists of an alteration at aposition corresponding to position 568. In one embodiment, the aminoacid at this position may substituted with Ala, Arg, Asn, Asp, Cys, Gln,Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val.In a particular embodiment, the variant comprises or consists of thesubstitution G568A of the mature polypeptide of SEQ ID NO:6.

In one aspect, the variant comprises or consists of an alteration at aposition corresponding to position 578. In one embodiment, the aminoacid at this position may substituted with Ala, Arg, Asn, Asp, Cys, Gln,Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val.In a particular embodiment, the variant comprises or consists of thesubstitution S578K or S578R of the mature polypeptide of SEQ ID NO:6.

In one aspect, the variant comprises or consists of an alteration at aposition corresponding to position 579. In one embodiment, the aminoacid at this position may substituted with Ala, Arg, Asn, Asp, Cys, Gln,Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val.In a particular embodiment, the variant comprises or consists of thesubstitution S579R or S579K of the mature polypeptide of SEQ ID NO:6. Apreferred substitution at a position corresponding to position 579 isS579R.

In one aspect, the variant comprises or consists of an alteration at aposition corresponding to position 664. In one embodiment, the aminoacid at this position may substituted with Ala, Arg, Asn, Asp, Cys, Gln,Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val.In a particular embodiment, the variant comprises or consists of thesubstitution T664K of the mature polypeptide of SEQ ID NO:6.

In one aspect, the variant comprises or consists of an alteration at aposition corresponding to position 672. In one embodiment, the aminoacid at this position may substituted with Ala, Arg, Asn, Asp, Cys, Gln,Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val.In a particular embodiment, the variant comprises or consists of thesubstitution N672D of the mature polypeptide of SEQ ID NO:6.

In one aspect, the variant comprises or consists of an alteration at aposition corresponding to position 885. In one embodiment, the aminoacid at this position may substituted with Ala, Arg, Asn, Asp, Cys, Gln,Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val.In a particular embodiment, the variant comprises or consists of thesubstitution K855R of the mature polypeptide of SEQ ID NO:6.

In one aspect, the variant comprises or consists of an alteration at aposition corresponding to position 887. In one embodiment, the aminoacid at this position may substituted with Ala, Arg, Asn, Asp, Cys, Gln,Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val.In a particular embodiment, the variant comprises or consists of thesubstitution K887R of the mature polypeptide of SEQ ID NO:6.

In one aspect, the variant comprises or consists of an alteration at aposition corresponding to position 892. In one embodiment, the aminoacid at this position may substituted with Ala, Arg, Asn, Asp, Cys, Gln,Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, or Val.In a particular embodiment, the variant comprises or consists of thesubstitution N892Y, N892W or N892F of the mature polypeptide of SEQ IDNO:6. A preferred substitution at a position corresponding to position892 is N892Y.

In one embodiment the present invention relates to detergentcompositions comprising a xanthan lyase variant as described herein,having an alteration at one or more positions selected from the groupconsisting of positions: 9, 15, 46, 58, 66, 89, 95, 100, 106, 109, 183,188, 190, 203, 204, 221, 229, 234, 238, 240, 242, 243, 257, 258, 291,293, 316, 320, 324, 329, 333, 339, 341, 352, 354, 360, 377, 400, 419,450, 451, 454, 481, 492, 567, 568, 578, 579, 664, 672, 855, 887 and 892of SEQ ID NO:6, wherein each position corresponds to the positions ofSEQ ID NO:6.

In one embodiment the present invention relates to detergentcompositions comprising a xanthan lyase variant as described hereinhaving one or more substitutions selected from the group consisting of:K9R, N15T, L46D, A58L, S66H, Q89Y, K95E, S100D, N106Y, Q109R, Q109D,Q109F, Q109K, Q109A, K183Q,K183R, V188I, A190Q, A203P, K204R, A221P,E229N, E229S, I234V, I238W, I238L, I238M, I240W, N242S, G243V, Y257W,R258E, K291R, A293G, A293P, K316R, K320R, L324Q, K329R, K333R, L339M,I341P, V352I, S354P, K360R, F377Y, K400R, F419Y, D450P, K451E, K451R,A454V, K481R, A492L, K567R, G568A, S578K, S578R, S579R, S579K, T664K,N672D, K855R, K887R, N892Y, N892W and N892F, wherein numbering isaccording to SEQ ID NO:6.

Preferred are xanthan lyase variants having an alteration (e.g. asubstitution, deletion or insertion, preferably a substitution) in thepositions selected from the group consisting of positions: 190, 229,234, 440, 582, 624, 631, 635, 672, 703, 738, 752, 753, 754, 757, 769,775, 801, 875, 892, and any combination thereof, preferably229+672+752+753+769+775+801+875+892;229+672+753+754+769+775+801+875+892;229+672+752+753+754+769+775+801+875+892;190+229+234+624+672+753+754+769+775+801+875;190+229+631+672+703+752+753+769+775+801+875;190+229+234+582+672+753+754+757+769+775+801+875+892; or229+440+582+624+635+672+738+753+754+757+769+775+801+875+892 of SEQ IDNO:6.

Particularly preferred are xanthan lyase variants having an alteration(e.g. a substitution, deletion or insertion, preferably a substitution)selected from the group consisting of the following alterations:E229N+N672D+P752K+G753E+A769D+L775A+D801G+K875T+N892Y;E2295+N672D+G753E+5754E+A769D+L775A+D801G+K875T+N892Y;E2295+N672D+P752R+G753E+S754E+A769D+L775A+D801G+K875T+N892Y;A190Q+E229S+I234V+A624E+N672D+G753E+S754E+A769D+L775A+D801G+K875T;A190Q+E229S+T631N+N672D+1703L+P752K+G753E+A769D+L775A+D801G+K875T;A190Q+E229S+1234V+S582K+N672D+G753E+S754E+S757D+A769D+L775A+D801G+K875T+N892Y;E229S+N440K+S582K+A624E+S635E+N672D+G738L+G753E+S754E+S757D+A769D+L775A+D801G+K875T+N892Y; andS100D+E229S+K360G+D458S+S582K+N672D+G753E+S754E+S757D+A769D+L775A+D801G+A843P+K875T+N892Y+N1008D.

All of the above alterations are relative to SEQ ID NO:6.

In a particular embodiment, the invention relates to a detergentcomposition comprising a xanthan lyase variant selected from the groupconsisting of the xanthan lyase variants set forth in Table 17 herein.

In a particular embodiment, the invention relates to a detergentcomposition comprising a xanthan lyase variant selected from the groupconsisting of the xanthan lyase variants set forth in Table 18 herein.

In a particular embodiment, the invention relates to a detergentcomposition comprising a xanthan lyase variant selected from the groupconsisting of the xanthan lyase variants set forth in Table 19 herein.

In a particular embodiment, the invention relates to a detergentcomposition comprising a xanthan lyase variant selected from the groupconsisting of the xanthan lyase variants set forth in Table 20 herein.

In a particular embodiment, the invention relates to a detergentcomposition comprising a xanthan lyase variant selected from the groupconsisting of the xanthan lyase variants set forth in Table 21 herein.

In a particular embodiment, the invention relates to a detergentcomposition comprising a xanthan lyase variant selected from the groupconsisting of the xanthan lyase variants set forth in Table 22 herein.

In a particular embodiment, the invention relates to a detergentcomposition comprising a xanthan lyase variant selected from the groupconsisting of the xanthan lyase variants set forth in Table 23 herein.

In a particular embodiment, the invention relates to a detergentcomposition comprising a xanthan lyase variant selected from the groupconsisting of the xanthan lyase variants set forth in Table 24 herein.

In a particular embodiment, the invention relates to a detergentcomposition comprising a xanthan lyase variant selected from the groupconsisting of the xanthan lyase variants set forth in Table 25 herein.

In a particular embodiment, the invention relates to a detergentcomposition comprising a xanthan lyase variant selected from the groupconsisting of the xanthan lyase variants set forth in Table 26 herein.

In a particular embodiment, the invention relates to a detergentcomposition comprising a xanthan lyase variant selected from the groupconsisting of the xanthan lyase variants set forth in Table 27 herein.

In a particular embodiment, the invention relates to a detergentcomposition comprising a xanthan lyase variant selected from the groupconsisting of the xanthan lyase variants set forth in Table 28 herein.

In a particular embodiment, the invention relates to a detergentcomposition comprising a xanthan lyase variant selected from the groupconsisting of the xanthan lyase variants set forth in Table 29 herein.

In a particular embodiment, the invention relates to detergentcompositions comprising a xanthan lyase variant selected from the groupconsisting of the xanthan lyase variants set forth in Table 30 herein.

In a particular embodiment, the invention relates to detergentcompositions comprising a xanthan lyase variant selected from the groupconsisting of the xanthan lyase variants set forth in Table 31 herein.

In a particular embodiment, the invention relates to detergentcompositions comprising a xanthan lyase variant selected from the groupconsisting of the xanthan lyase variants set forth in Table 32 herein.

In a particular embodiment, the invention relates to detergentcompositions comprising a xanthan lyase variant selected from the groupconsisting of the xanthan lyase variants set forth in Table 33 herein.

In various embodiments, the preferred endoglucanase variants arecombined with the preferred xanthan lyase variants. In some aspects, thedetergent composition thus comprises

(A) an endoglucanase variant selected from those having at least 61%,62%, 63%, 64%, 65%, 66%, 67%, 68%, 69%, 70%, 71%, 72%, 73%, 74%, 75%,76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%,90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, or 98% sequence identity to SEQID NO:2 and having an alteration (e.g. a substitution, deletion orinsertion, preferably a substitution) in the positions selected from thegroup consisting of positions: 559+579+697; 512+559+579+697;18+71+186+408+579+602+651+688+756;18+189+408+559+579+688+697+756+921+934; 313+488; 880+905+921+934;302+313+408+579+602+651+697+880+921+934; or216+313+408+476+579+602+638+651+697+719+880+887+921+934 of SEQ ID NO:2,preferably having an alteration selected from the group consisting ofthe following alterations: (A1) A559N+Y579W+T697G; (A2)K512P+A559N+Y579W+T697G; (A3)N18G+A71E+A186P+E408D+Y579W+1602T+A651P+A688G+V756Y; (A4)N18G+N189K+E408D+A559N+Y579W+A688G+T697G+V756Y+K921R+Y934G; (A5)S313D+E408D; (A6) R880K+N905D+K921R+Y934G; (A7)I302D+S313D+E408D+Y579W+I602T+A651P+T697G+R880K+K921R+Y934G; and (A8)N216Q+S313D+E408D+D476R+Y579W+I602T+F638N+A651P+T697G+W719R+R880K+T887K+K921R+Y934G of SEQ ID NO:2; and

(B) a xanthan lyase variant having at least 61%, 62%, 63%, 64%, 65%,66%, 67%, 68%, 69%, 70%, 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%,80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%,94%, 95%, 96%, 97%, or 98% sequence identity to SEQ ID NO:6 and havingan alteration (e.g. a substitution, deletion or insertion, preferably asubstitution) in the positions selected from the group consisting ofpositions: 229+672+752+753+769+775+801+875+892;229+672+753+754+769+775+801+875+892;229+672+752+753+754+769+775+801+875+892;190+229+234+624+672+753+754+769+775+801+875;190+229+631+672+703+752+753+769+775+801+875;190+229+234+582+672+753+754+757+769+775+801+875+892;229+440+582+624+635+672+738+753+754+757+769+775+801+875+892; or100+229+360+458+582+672+753+754+757+769+775+801+843+875+892+1008 of SEQID NO:6, preferably having an alteration selected from the groupconsisting of the following alterations: (B1)E229N+N672D+P752K+G753E+A769D+L775A+D801G+K875T+N892Y; (B2)E229S+N672D+G753E+5754E+A769D+L775A+D801G+K875T+N892Y; (B3)E229S+N672D+P752R+G753E+S754E+A769D+L775A+D801G+K875T+N892Y; (B4)A190Q+E229S+I234V+A624E+N672D+G753E+S754E+A769D+L775A+D801G+K875T; (B5)A190Q+E229S+T631N+N672D+I703L+P752K+G753E+A769D+L775A+D801G+K875T; (B6)A190Q+E229S+1234V+S582K+N672D+G753E+S754E+S757D+A769D+L775A+D801G+K875T+N892Y; (B7)E229S+N440K+S582K+A624E+S635E+N672D+G738L+G753E+S754E+S757D+A769D+L775A+D801G+K875T+N892Y; and (B8)S100D+E229S+K360G+D458S+S582K+N672D+G753E+S754E+S757D+A769D+L775A+D801G+A843P+K875T+N892Y+N1008D of SEQ ID NO:6.

In some aspects, the endoglucanase and/or the xanthan lyase variant donot comprise any further substitution besides those explicitly mentionedabove, i.e. the remainder of the sequence is identical to that of theparent enzyme as set forth in SEQ ID NO:2 and SEQ ID NO:6, respectively.

In some aspects, in the detergent compositions of the invention theendoglucanase variant A1, as defined above, can be combined with any oneof the xanthan lyase variants B1, B2, B3, B4, B5, B6, B7 and B8, asdefined above, wherein the respective enzymes preferably have at least90%, preferably at least 91%, 92%, 93%, 94%, 95%, 96% or 97% sequenceidentity to SEQ ID NO:2 and SEQ ID NO:6, respectively, preferably areidentical to their respective parent sequence with the exception of thesubstitutions explicitly mentioned herein.

In some aspects, in the detergent compositions of the invention theendoglucanase variant A2, as defined above, can be combined with any oneof the xanthan lyase variants B1, B2, B3, B4, B5, B6, B7 and B8, asdefined above, wherein the respective enzymes preferably have at least90%, preferably at least 91%, 92%, 93%, 94%, 95%, 96% or 97% sequenceidentity to SEQ ID NO:2 and SEQ ID NO:6, respectively, preferably areidentical to their respective parent sequence with the exception of thesubstitutions explicitly mentioned herein.

In some aspects, in the detergent compositions of the invention theendoglucanase variant A3, as defined above, can be combined with any oneof the xanthan lyase variants B1, B2, B3, B4, B5, B6, B7 and B8, asdefined above, wherein the respective enzymes preferably have at least90%, preferably at least 91%, 92%, 93%, 94%, 95%, 96% or 97% sequenceidentity to SEQ ID NO:2 and SEQ ID NO:6, respectively, preferably areidentical to their respective parent sequence with the exception of thesubstitutions explicitly mentioned herein.

In some aspects, in the detergent compositions of the invention theendoglucanase variant A4, as defined above, can be combined with any oneof the xanthan lyase variants B1, B2, B3, B4, B5, B6, B7 and B8, asdefined above, wherein the respective enzymes preferably have at least90%, preferably at least 91%, 92%, 93%, 94%, 95%, 96% or 97% sequenceidentity to SEQ ID NO:2 and SEQ ID NO:6, respectively, preferably areidentical to their respective parent sequence with the exception of thesubstitutions explicitly mentioned herein.

In some aspects, in the detergent compositions of the invention theendoglucanase variant A5, as defined above, can be combined with any oneof the xanthan lyase variants B1, B2, B3, B4, B5, B6, B7 and B8, asdefined above, wherein the respective enzymes preferably have at least90%, preferably at least 91%, 92%, 93%, 94%, 95%, 96% or 97% sequenceidentity to SEQ ID NO:2 and SEQ ID NO:6, respectively, preferably areidentical to their respective parent sequence with the exception of thesubstitutions explicitly mentioned herein.

In some aspects, in the detergent compositions of the invention theendoglucanase variant A6, as defined above, can be combined with any oneof the xanthan lyase variants B1, B2, B3, B4, B5, B6, B7 and B8, asdefined above, wherein the respective enzymes preferably have at least90%, preferably at least 91%, 92%, 93%, 94%, 95%, 96% or 97% sequenceidentity to SEQ ID NO:2 and SEQ ID NO:6, respectively, preferably areidentical to their respective parent sequence with the exception of thesubstitutions explicitly mentioned herein.

In some aspects, in the detergent compositions of the invention theendoglucanase variant A7, as defined above, can be combined with any oneof the xanthan lyase variants B1, B2, B3, B4, B5, B6, B7 and B8, asdefined above, wherein the respective enzymes preferably have at least90%, preferably at least 91%, 92%, 93%, 94%, 95%, 96% or 97% sequenceidentity to SEQ ID NO:2 and SEQ ID NO:6, respectively, preferably areidentical to their respective parent sequence with the exception of thesubstitutions explicitly mentioned herein.

In some aspects, in the detergent compositions of the invention theendoglucanase variant A8, as defined above, can be combined with any oneof the xanthan lyase variants B1, B2, B3, B4, B5, B6, B7 and B8, asdefined above, wherein the respective enzymes preferably have at least90%, preferably at least 91%, 92%, 93%, 94%, 95%, 96% or 97% sequenceidentity to SEQ ID NO:2 and SEQ ID NO:6, respectively, preferably areidentical to their respective parent sequence with the exception of thesubstitutions explicitly mentioned herein.

If not explicitly indicated otherwise, all the variants described abovemay further comprise one or more additional alterations at one or more(e.g. several) other positions in any of the regions described herein.

The amino acid changes may be of a minor nature, that is conservativeamino acid substitutions or insertions that do not significantly affectthe folding and/or activity of the protein; small deletions, typicallyof 1-30 amino acids; small amino- or carboxyl-terminal extensions, suchas an amino-terminal methionine residue; a small linker peptide of up to20-25 residues; or a small extension that facilitates purification bychanging net charge or another function, such as a poly-histidine tract,an antigenic epitope or a binding domain.

Examples of conservative substitutions are within the groups of basicamino acids (arginine, lysine and histidine), acidic amino acids(glutamic acid and aspartic acid), polar amino acids (glutamine andasparagine), hydrophobic amino acids (leucine, isoleucine and valine),aromatic amino acids (phenylalanine, tryptophan and tyrosine), and smallamino acids (glycine, alanine, serine, threonine and methionine). Aminoacid substitutions that do not generally alter specific activity areknown in the art and are described, for example, by H. Neurath and R.L.Hill, 1979, In, The Proteins, Academic Press, New York. Commonsubstitutions are Ala/Ser, Val/Ile, Asp/Glu, Thr/Ser, Ala/Gly, Ala/Thr,Ser/Asn, Ala/Val, Ser/Gly, Tyr/Phe, Ala/Pro, Lys/Arg, Asp/Asn, Leu/Ile,LeuNal, Ala/Glu, and Asp/Gly.

Alternatively, the amino acid changes are of such a nature that thephysico-chemical properties of the polypeptides are altered. Forexample, amino acid changes may improve the thermal stability of thepolypeptide, alter the substrate specificity, change the pH optimum, andthe like.

Essential amino acids in a polypeptide can be identified according toprocedures known in the art, such as site-directed mutagenesis oralanine-scanning mutagenesis (Cunningham and Wells, 1989, Science 244:1081-1085). In the latter technique, single alanine mutations areintroduced at every residue in the molecule, and the resultant mutantmolecules are tested for xanthan lyase activity to identify amino acidresidues that are critical to the activity of the molecule. See also,Hilton et al., 1996, J. Biol. Chem. 271: 4699-4708. The active site ofthe enzyme or other biological interaction can also be determined byphysical analysis of structure, as determined by such techniques asnuclear magnetic resonance, crystallography, electron diffraction, orphotoaffinity labeling, in conjunction with mutation of putative contactsite amino acids. See, for example, de Vos et al., 1992, Science 255:306-312; Smith et al., 1992, J. Mol. Biol. 224: 899-904; Wlodaver etal., 1992, FEBS Lett. 309: 59-64. The identity of essential amino acidscan also be inferred from an alignment with a related polypeptide.

In one embodiment, the present invention relates to a detergentcomposition comprising an endoglucanase variant of the invention, havingthe total number of alterations compared to SEQ ID NO:2 between 1 and20, e.g. between 1 and 18 or between 5 and 15 or between 8 and 14, suchas 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14 or 15 alterations, anda xanthan lyase variant as described herein, having a total number ofalterations compared to SEQ ID NO:6 between 1 and 20, e.g.

between 1 and 18 or between 5 and 17 or between 10 and 16, such as 1, 2,3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17 or 18 alterations.

In one embodiment, the present invention relates to a detergentcomposition comprising an endoglucanase variant of the invention and axanthan lyase variant of the invention, having an activity on xanthangum, preferably said activity on xanthan gum is a xanthan gum degradingactivity, further preferably said xanthan gum degrading activity is EC4.2.2.12 activity and endoglucanase EC 3.2.1.4 activity.

In an embodiment, the variant has an improved stability in a detergentcomposition compared to a parent enzyme (e.g. SEQ ID NO:2 or 6). In oneembodiment, the improved stability is measured as an improved half-life.In one embodiment, the improved stability is measured as half-lifeimprovement factor.

In one embodiment, the present invention relates to a detergentcomposition comprising an endoglucanase/xanthan lyase variant of theinvention, wherein said variant has an improved stability in a detergentcomposition compared to a parent enzyme (e.g. with SEQ ID NO:2 or 6);preferably said detergent composition comprises a chelator; furtherpreferably said chelator is EDTA or citrate.

In one embodiment, the present invention relates to a detergentcomposition comprising an endoglucanase/xanthan lyase variant of theinvention, wherein said variant has a half-life improvement factor (HIF)1.0; preferably said variant has a half-life improvement factor(HIF) >1.0, preferably at least 1.2, such as at least 1.5, e.g. at least2.0, relative to a parent endoglucanase/xanthan lyase. A preferred wayof calculating said half-life improvement factor (HIF) is described inExamples 3 and 7 herein. Accordingly, residual activity (RA) can becalculated using the following formula: RA (%)=(Abs(Stress)/Abs(Ref) x100%, wherein Abs(Stress) is the absorbance at 405 nm of the sample inthe stress microtiter plate (MTP) (e.g. incubated at 25° C. over-nightor any other combination of temperature and time) after subtractingrelevant background absorbance contributions, Abs(Ref) is the absorbanceat 405 nm of the sample in the reference MTP (e.g. incubated at 4° C.over-night) after subtracting relevant background absorbancecontributions, wherein half-lives for the degradation of each variantand parent endoglucanase/xanthan lyase (e.g. at 25° C.) are calculatedusing the following formula: T½ (variant or parent)=(Ln (0.5)/Ln(RA-variant/100))*time, wherein “RA” is the residual activity in percentand “Time” is the incubation time in hours for both the stress andreference MTP, wherein half-life-improvement factors (HIFs) arecalculated using the following formula: HIF =T½ (variant)/T½(wild-type), e.g. wherein T½ wt (or wild type) is the T½ of the matureparent endoglucanase/xanthan lyase with SEQ ID NO:2 or 6.

In one embodiment the present invention relates to a detergentcomposition comprising an endoglucanase/xanthan lyase variant of theinvention, wherein a half-life improvement factor (HIF) is determinedafter incubation of said endoglucanase/xanthan lyase variant in adetergent composition at 25° C. or 30° C. for a time period from about30 min to about 20 h.

Parent

The parent endoglucanase may be (a) a polypeptide having at least 60%sequence identity to the mature polypeptide of SEQ ID NO:2 or 6; (b) apolypeptide encoded by a polynucleotide that hybridizes under lowstringency conditions with (i) the mature polypeptide coding sequence ofSEQ ID NO:1 or 5, or (ii) the full-length complement of (i); or (c) apolypeptide encoded by a polynucleotide having at least 60% sequenceidentity to the mature polypeptide coding sequence of SEQ ID NO:1 or 5.

In an aspect, the parent has a sequence identity to the maturepolypeptide of SEQ ID NO:2 or 6 of at least 60%, e.g. at least 65%, 70%,75%, 80%, 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or100%, which have endoglucanase/xanthan lyase activity. In one aspect,the amino acid sequence of the parent differs by up to 10 amino acids,e.g. 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10, from the mature polypeptide ofSEQ ID NO:2 or 6.

In another aspect, the parent endoglucanase comprises or consists of theamino acid sequence of SEQ ID NO:2. In another aspect, the parentendoglucanase comprises or consists of the mature polypeptide of SEQ IDNO:2. In another aspect, the parent endoglucanase is a fragment of themature polypeptide of SEQ ID NO:2 containing at least 85%, 86%, 87%,88%, 89%, 90%, 91%, 92%, 93%, 94% or 95% of the number of amino acids ofSEQ ID NO:2. In another embodiment, the parent endoglucanase is anallelic variant of the mature polypeptide of SEQ ID NO:2.

In another aspect, the parent xanthan lyase comprises or consists of theamino acid sequence of SEQ ID NO:6. In another aspect, the parentxanthan lyase comprises or consists of the mature polypeptide of SEQ IDNO:6. In another aspect, the parent xanthan lyase is a fragment of themature polypeptide of SEQ ID NO:6 containing at least 85%, 86%, 87%,88%, 89%, 90%, 91%, 92%, 93%, 94% or 95% of the number of amino acids ofSEQ ID NO:6. In another embodiment, the parent xanthan lyase is anallelic variant of the mature polypeptide of SEQ ID NO:6.

In another aspect, the parent is encoded by a polynucleotide thathybridizes under very low stringency conditions, low stringencyconditions, medium stringency conditions, medium-high stringencyconditions, high stringency conditions, or very high stringencyconditions with (i) the mature polypeptide coding sequence of SEQ IDNO:1 or 5, or (ii) the full-length complement of (i) (Sambrook et al.,1989, Molecular Cloning, A Laboratory Manual, 2d edition, Cold SpringHarbor, N.Y.).

The polynucleotide of SEQ ID NO:1 or 5 or a subsequence thereof, as wellas the polypeptide of SEQ ID NO:2 or 6 or a fragment thereof, may beused to design nucleic acid probes to identify and clone DNA encoding aparent from strains of different genera or species according to methodswell known in the art. In particular, such probes can be used forhybridization with the genomic DNA or cDNA of a cell of interest,following standard Southern blotting procedures, in order to identifyand isolate the corresponding gene therein. Such probes can beconsiderably shorter than the entire sequence, but should be at least15, e.g. at least 25, at least 35, or at least 70 nucleotides in length.Preferably, the nucleic acid probe is at least 100 nucleotides inlength, e.g. at least 200 nucleotides, at least 300 nucleotides, atleast 400 nucleotides, at least 500 nucleotides, at least 600nucleotides, at least 700 nucleotides, at least 800 nucleotides, or atleast 900 nucleotides in length. Both DNA and RNA probes can be used.The probes are typically labeled for detecting the corresponding gene(for example, with ³²P, ³H, ³⁵S, biotin, or avidin). Such probes areencompassed by the present invention.

A genomic DNA or cDNA library prepared from such other strains may bescreened for DNA that hybridizes with the probes described above andencodes a parent. Genomic or other DNA from such other strains may beseparated by agarose or polyacrylamide gel electrophoresis, or otherseparation techniques. DNA from the libraries or the separated DNA maybe transferred to and immobilized on nitrocellulose or other suitablecarrier material. In order to identify a clone or DNA that hybridizeswith SEQ ID NO:1 or 5 or a subsequence thereof, the carrier material isused in a Southern blot.

For purposes of the present invention, hybridization indicates that thepolynucleotide hybridizes to a labeled nucleic acid probe correspondingto (i) SEQ ID NO:1 or 5; (ii) the mature polypeptide coding sequence ofSEQ ID NO:1 or 5; (iii) the full-length complement thereof; or (iv) asubsequence thereof; under very low to very high stringency conditions.Molecules to which the nucleic acid probe hybridizes under theseconditions can be detected using, for example, X-ray film or any otherdetection means known in the art.

In one aspect, the nucleic acid probe is the mature polypeptide codingsequence of SEQ ID NO:1 or 5. In another aspect, the nucleic acid probeis a polynucleotide that encodes the polypeptide of SEQ ID NO:2 or 6;the mature polypeptide thereof; or a fragment thereof. In anotheraspect, the nucleic acid probe is SEQ ID NO:1 or 5.

In another embodiment, the parent is encoded by a polynucleotide havinga sequence identity to the mature polypeptide coding sequence of SEQ IDNO:1 or 5 of at least 60%, e.g. at least 65%, 70%, 75%, 80%, 85%, 90%,91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100%.

The polypeptide may be a hybrid polypeptide in which a region of onepolypeptide is fused at the N-terminus or the C-terminus of a region ofanother polypeptide.

The parent may be a fusion polypeptide or cleavable fusion polypeptidein which another polypeptide is fused at the N-terminus or theC-terminus of the polypeptide of the present invention. A fusionpolypeptide is produced by fusing a polynucleotide encoding anotherpolypeptide to a polynucleotide of the present invention. Techniques forproducing fusion polypeptides are known in the art, and include ligatingthe coding sequences encoding the polypeptides so that they are in frameand that expression of the fusion polypeptide is under control of thesame promoter(s) and terminator. Fusion polypeptides may also beconstructed using intein technology in which fusion polypeptides arecreated post-translationally (Cooper et al., 1993, EMBO J. 12:2575-2583; Dawson et al., 1994, Science 266: 776-779).

A fusion polypeptide can further comprise a cleavage site between thetwo polypeptides. Upon secretion of the fusion protein, the site iscleaved releasing the two polypeptides. Examples of cleavage sitesinclude, but are not limited to, the sites disclosed in Martin et al.,2003, J. Ind. Microbiol. Biotechnol. 3: 568-576; Svetina et al., 2000,J. Biotechnol. 76: 245-251; Rasmussen-Wilson et al., 1997, Appl.Environ. Microbiol. 63: 3488-3493; Ward et al., 1995, Biotechnology 13:498-503; and Contreras et al., 1991, Biotechnology 9: 378-381; Eaton etal., 1986, Biochemistry 25: 505-512; Collins-Racie et al., 1995,Biotechnology 13: 982-987; Carter et al., 1989, Proteins: Structure,Function, and Genetics 6: 240-248; and Stevens, 2003, Drug DiscoveryWorld 4: 35-48.

The parent may be obtained from microorganisms of any genus. Forpurposes of the present invention, the term “obtained from” as usedherein in connection with a given source shall mean that the parentencoded by a polynucleotide is produced by the source or by a strain inwhich the polynucleotide from the source has been inserted. In oneaspect, the parent is secreted extracellularly.

The parent may be a bacterial enzyme. For example, the parent may be aGram-positive bacterial polypeptide such as a Bacillus, Clostridium,Enterococcus, Geobacillus, Lactobacillus, Lactococcus, Oceanobacillus,Staphylococcus, Streptococcus, or Streptomyces enzyme, or aGram-negative bacterial polypeptide such as a Campylobacter, E. coli,Flavobacterium, Fusobacterium, Helicobacter, Ilyobacter, Neisseria,Pseudomonas, Salmonella, or Ureaplasma enzyme.

In one aspect, the parent is a Bacillus alkalophilus, Bacillusamyloliquefaciens, Bacillus brevis, Bacillus circulans, Bacillusclausfi, Bacillus coagulans, Bacillus firmus, Bacillus lautus, Bacilluslentus, Bacillus licheniformis, Bacillus megaterium, Bacillus pumilus,Bacillus stearothermophilus, Bacillus subtilis, or Bacillusthuringiensis enzyme.

In another aspect, the parent is a Streptococcus equisimilis,Streptococcus pyogenes, Streptococcus uberis, or Streptococcus equisubsp. Zooepidemicus enzyme.

In another aspect, the parent is a Streptomyces achromogenes,Streptomyces avermitilis, Streptomyces coelicolor, Streptomyces griseus,or Streptomyces lividans enzyme.

The parent may be a fungal enzyme. For example, the parent may be ayeast enzyme such as a Candida, Kluyveromyces, Pichia, Saccharomyces,Schizosaccharomyces, or Yarrowia enzyme; or a filamentous fungal enzymesuch as an Acremonium, Agaricus, Alternaria, Aspergillus, Aureobasidium,Botryospaeria, Ceriporiopsis, Chaetomidium, Chrysosporium, Claviceps,Cochliobolus, Coprinopsis, Coptotermes, Corynascus, Cryphonectria,Cryptococcus, Dipodia, Exidia, Filibasidium, Fusarium, Gibberella,Holomastigotoides, Humicola, Irpex, Lentinula, Leptospaeria,Magnaporthe, Melanocarpus, Meripilus, Mucor, Myceliophthora,Neocallimastix, Neurospora, Paecilomyces, Penicillium, Phanerochaete,Piromyces, Poitrasia, Pseudoplectania, Pseudotrichonympha, Rhizomucor,Schizophyllum, Scytalidium, Talaromyces, Thermoascus, Thielavia,Tolypocladium, Trichoderma, Trichophaea, Verticillium, Volvariella, orXylaria enzyme.

In another aspect, the parent is a Saccharomyces carlsbergensis,Saccharomyces cerevisiae, Saccharomyces diastaticus, Saccharomycesdouglasfi, Saccharomyces kluyveri, Saccharomyces norbensis, orSaccharomyces oviformis enzyme.

In another aspect, the parent is an Acremonium cellulolyticus,Aspergillus aculeatus, Aspergillus awamori, Aspergillus foetidus,Aspergillus fumigatus, Aspergillus japonicus, Aspergillus nidulans,Aspergillus niger, Aspergillus oryzae, Chrysosporium inops,Chrysosporium keratinophilum, Chrysosporium lucknowense, Chrysosporiummerdarium, Chrysosporium pannicola, Chrysosporium queenslandicum,Chrysosporium tropicum, Chrysosporium zonatum, Fusarium bactridioides,Fusarium cerealis, Fusarium crookwellense, Fusarium culmorum, Fusariumgraminearum, Fusarium graminum, Fusarium heterosporum, Fusarium negundi,Fusarium oxysporum, Fusarium reticulatum, Fusarium roseum, Fusariumsambucinum, Fusarium sarcochroum, Fusarium sporotrichioides, Fusariumsulphureum, Fusarium torulosum, Fusarium trichothecioides, Fusariumvenenatum, Humicola grisea, Humicola insolens, Humicola lanuginosa,lrpex lacteus, Mucor miehei, Myceliophthora thermophila, Neurosporacrassa, Penicillium funiculosum, Penicillium purpurogenum, Phanerochaetechtysosporium, Thielavia achromatica, Thielavia albomyces, Thielaviaalbopilosa, Thielavia australeinsis, Thielavia fimeti, Thielaviamicrospora, Thielavia ovispora, Thielavia peruviana, Thielavia setosa,Thielavia spededonium, Thielavia subthermophila, Thielavia terrestris,Trichoderma harzianum, Trichoderma koningii, Trichodermalongibrachiatum, Trichoderma reesei, or Trichoderma viride enzyme.

In another aspect, the parent is a Paenibacillus sp. xanthan lyase, e.g.the xanthan lyase of SEQ ID NO:6.

In another aspect, the parent is a Paenibacillus sp. Endoglucanase ofthe GH9 family, e.g. the endoglucanase of SEQ ID NO:2.

It will be understood that for the aforementioned species, the inventionencompasses both the perfect and imperfect states, and other taxonomicequivalents, e.g. anamorphs, regardless of the species name by whichthey are known. Those skilled in the art will readily recognize theidentity of appropriate equivalents.

Strains of these species are readily accessible to the public in anumber of culture collections, such as the American Type CultureCollection (ATCC), Deutsche SammLung von Mikroorganismen andZellkulturen GmbH (DSMZ), Centraalbureau Voor Schimmelcultures (CBS),and Agricultural Research Service Patent Culture Collection, NorthernRegional Research Center (NRRL).

The parent may be identified and obtained from other sources includingmicroorganisms isolated from nature (e.g. soil, composts, water, etc.)or DNA samples obtained directly from natural materials (e.g. soil,composts, water, etc.) using the above-mentioned probes. Techniques forisolating microorganisms and DNA directly from natural habitats are wellknown in the art. A polynucleotide encoding a parent may then beobtained by similarly screening a genomic DNA or cDNA library of anothermicroorganism or mixed DNA sample. Once a polynucleotide encoding aparent has been detected with the probe(s), the polynucleotide can beisolated or cloned by utilizing techniques that are known to those ofordinary skill in the art (see, e.g. Sambrook et al., 1989, supra).

Embodiments

In one embodiment, the present invention relates to a detergentcomposition comprising at least one (e.g. 1, 2, 3, 4, 5, 6, 7, 8, 9 or10) endoglucanase variant as described herein and at least one (e.g. 1,2, 3, 4, 5, 6, 7, 8, 9 or 10) xanthan lyase variant as described herein.

In one embodiment, the detergent composition of the invention furthercomprises one or more additional enzymes selected from the groupconsisting of: proteases, amylases, lichenases, lipases, cutinases,cellulases, endoglucanases, xyloglucanases, pectinases, pectin lyases,xanthanases, peroxidases, haloperoxygenases, catalases and mannanases,or any mixture thereof.

The detergent compositions of the invention may further comprise one ormore detergent components. In some embodiments said detergent componentmay be a chelator, such as EDTA or citrate.

In one embodiment, the detergent composition further comprises one ormore detergent components, wherein said detergent composition is in formof a bar, a homogenous tablet, a tablet having two or more layers, apouch having one or more compartments, a regular or compact powder, agranule, a paste, a gel, or a regular, compact or concentrated liquid.

In one embodiment, the present invention relates to use of a detergentcomposition of the invention, wherein said use is selected from thegroup of: use for degrading xanthan gum and use in a cleaning process,such as laundry or hard surface cleaning such as dish wash.

In one embodiment, the present invention relates to use of a detergentcomposition of the invention, wherein said composition has an enzymedetergency benefit.

In one embodiment the present invention relates to a method fordegrading xanthan gum comprising: applying a detergent composition ofthe invention to a xanthan gum.

In one embodiment the present invention relates to a method fordegrading xanthan gum comprising: applying a detergent composition ofthe invention to a xanthan gum, wherein said xanthan gum is on thesurface of a textile or hard surface, such as dish wash.

Compositions

In one certain aspect, the variants according to the invention haveimproved stability in detergents compared to a parent enzyme or comparedto an endoglucanase/xanthan lyase having the identical amino acidsequence of the variant, but not having an alteration (e.g. asubstitution, deletion or insertion, preferably a substitution) at oneor more of the specified positions or compared to the endoglucanase withthe amino acid sequence set forth in SEQ ID NO:2 or the xanthan lyasewith the amino aicd sequence as set forth in SEQ ID NO:6, whereinactivity and/or stability in detergent is measured as disclosed inExamples 3 and 7 herein.

Besides enzymes the detergent compositions may comprise additionalcomponents. The choice of additional components is within the skill ofthe artisan and includes conventional ingredients, including theexemplary non-limiting components set forth below. The choice ofcomponents may include, for fabric care, the consideration of the typeof fabric to be cleaned, the type and/or degree of soiling, thetemperature at which cleaning is to take place, and the formulation ofthe detergent product. Although components mentioned below arecategorized by general header according to a particular functionality,this is not to be construed as a limitation, as a component may compriseadditional functionalities as will be appreciated by the skilledartisan.

The detergent composition may be suitable for the laundring of textilessuch as e.g. fabrics, cloths or linen, or for cleaning hard surfacessuch as e.g. floors, tables, or dish wash.

Detergent Compositions

In one embodiment, an endoglucanase variant as described herein may beadded to a detergent composition in an amount corresponding to0.0001-200 mg of enzyme protein, such as 0.0005-100 mg of enzymeprotein, preferably 0.001-30 mg of enzyme protein, more preferably0.005-8 mg of enzyme protein, even more preferably 0.01-2 mg of enzymeprotein per litre of wash liquor.

In one embodiment, an xanthan lyase variant as described herein may beadded to a detergent composition in an amount corresponding to0.0001-200 mg of enzyme protein, such as 0.0005-100 mg of enzymeprotein, preferably 0.001-30 mg of enzyme protein, more preferably0.005-8 mg of enzyme protein, even more preferably 0.01-2 mg of enzymeprotein per litre of wash liquor.

In some embodiments, each an endoglucanase variant as described hereinand a xanthan lyase variant as described herein may be added to adetergent composition each in an amount corresponding to 0.0001-200 mgof enzyme protein, such as 0.0005-100 mg of enzyme protein, preferably0.001-30 mg of enzyme protein, more preferably 0.005-8 mg of enzymeprotein, even more preferably 0.01-2 mg of enzyme protein per litre ofwash liquor.

A composition for use in automatic dishwash (ADW), for example, mayinclude 0.0001-50%, such as 0.001-20%, such as 0.01-10%, such as 0.05-5%of each of the enzyme proteins by weight of the composition.

A composition for use in laundry granulation or a solid/granular laundrycompositon in general, for example, may include 0.0001-50%, such as0.001-20%, such as 0.01-10%, such as 0.05-5% of each of the enzymeproteins by weight of the composition.

A composition for use in laundry liquid, for example, may include0.0001-10%, such as 0.001-7%, such as 0.1-5% of each of the enzymeproteins by weight of the composition.

The enzyme(s) of the detergent composition of the invention may bestabilized using conventional stabilizing agents, e.g. a polyol such aspropylene glycol or glycerol, a sugar or sugar alcohol, lactic acid,boric acid, or a boric acid derivative, e.g. an aromatic borate ester,or a phenyl boronic acid derivative such as 4-formylphenyl boronic acid,and the composition may be formulated as described in, for example,WO92/19709 and WO92/19708.

In certain markets different wash conditions and, as such, differenttypes of detergents are used. This is disclosed in e.g. EP1025240. Forexample, In Asia (Japan) a low detergent concentration system is used,while the United States uses a medium detergent concentration system,and Europe uses a high detergent concentration system.

A low detergent concentration system includes detergents where less thanabout 800 ppm of detergent components are present in the wash water.Japanese detergents are typically considered low detergent concentrationsystem as they have approximately 667 ppm of detergent componentspresent in the wash water.

A medium detergent concentration includes detergents where between about800 ppm and about 2000 ppm of detergent components are present in thewash water. North American detergents are generally considered to bemedium detergent concentration systems as they have approximately 975ppm of detergent components present in the wash water.

A high detergent concentration system includes detergents where greaterthan about 2000 ppm of detergent components are present in the washwater. European detergents are generally considered to be high detergentconcentration systems as they have approximately 4500-5000 ppm ofdetergent components in the wash water.

Latin American detergents are generally high suds phosphate builderdetergents and the range of detergents used in Latin America can fall inboth the medium and high detergent concentrations as they range from1500-6000 ppm of detergent components in the wash water. Such detergentcompositions are all embodiments of the invention.

A polypeptide of the present invention may also be incorporated in thedetergent formulations disclosed in WO97/07202, which is herebyincorporated by reference.

Surfactants

The detergent composition may comprise one or more surfactants, whichmay be anionic and/or cationic and/or non-ionic and/or semi-polar and/orzwitterionic, or a mixture thereof. In preferred embodiments, thedetergent compositions of the invention comprise at least onesurfactant. In a particular embodiment, the detergent compositionincludes a mixture of one or more nonionic surfactants and one or moreanionic surfactants. The surfactant(s) is typically present at a levelof from about 0.1-60% by weight, such as about 1-40%, or about 3-20%, orabout 3-10%. The surfactant(s) is chosen based on the desired cleaningapplication, and includes any conventional surfactant(s) known in theart. Any surfactant known in the art for use in detergents may beutilized.

When included therein the detergent will usually comprise from about1-40% by weight, such as from about 5-30%, including from about 5-15%,or from about 20-25% of an anionic surfactant. Non-limiting examples ofanionic surfactants include sulfates and sulfonates, in particular,linear alkylbenzenesulfonates (LAS), isomers of LAS, branchedalkylbenzenesulfonates (BABS), phenylalkanesulfonates,alpha-olefinsulfonates (AOS), olefin sulfonates, alkene sulfonates,alkane-2,3-diylbis(sulfates), hydroxyalkanesulfonates and disulfonates,alkyl sulfates (AS) such as sodium dodecyl sulfate (SDS), fatty alcoholsulfates (FAS), primary alcohol sulfates (PAS), alcohol ethersulfates(AES or AEOS or FES, also known as alcohol ethoxysulfates or fattyalcohol ether sulfates), secondary alkanesulfonates (SAS), paraffinsulfonates (PS), ester sulfonates, sulfonated fatty acid glycerolesters, alpha-sulfo fatty acid methyl esters (alpha-SFMe or SES)including methyl ester sulfonate (MES), alkyl- or alkenylsuccinic acid,dodecenyl/tetradecenyl succinic acid (DTSA), fatty acid derivatives ofamino acids, diesters and monoesters of sulfo-succinic acid or soap, andcombinations thereof.

When included therein the detergent will usually comprise from about0-10% by weight of a cationic surfactant. Non-limiting examples ofcationic surfactants include alklydimethylethanolamine quat (ADMEAQ),cetyltrimethylammonium bromide (CTAB), dimethyldistearylammoniumchloride (DSDMAC), and alkylbenzyldimethylammonium, alkyl quaternaryammonium compounds, alkoxylated quaternary ammonium (AQA) compounds, andcombinations thereof.

When included therein the detergent will usually comprise from about0.2-40% by weight of a non-ionic surfactant, for example from about0.5-30%, in particular from about 1-20%, from about 3-10%, such as fromabout 3-5%, or from about 8-12%. Non-limiting examples of non-ionicsurfactants include alcohol ethoxylates (AE or AEO), alcoholpropoxylates, propoxylated fatty alcohols (PFA), alkoxylated fatty acidalkyl esters, such as ethoxylated and/or propoxylated fatty acid alkylesters, alkylphenol ethoxylates (APE), nonylphenol ethoxylates (NPE),alkylpolyglycosides (APG), alkoxylated amines, fatty acidmonoethanolamides (FAM), fatty acid diethanolamides (FADA), ethoxylatedfatty acid monoethanolamides (EFAM), propoxylated fatty acidmonoethanolamides (PFAM), polyhydroxy alkyl fatty acid amides, or N-acylN-alkyl derivatives of glucosamine (glucamides, GA, or fatty acidglucamide, FAGA), as well as products available under the trade namesSPAN and TWEEN, and combinations thereof.

When included therein the detergent will usually comprise from about0-10% by weight of a semipolar surfactant. Non-limiting examples ofsemipolar surfactants include amine oxides (AO) such asalkyldimethylamineoxide, N-(coco alkyl)-N,N-dimethylamine oxide andN-(tallow-alkyl)-N,N-bis(2-hydroxyethyl)amine oxide, fatty acidalkanolamides and ethoxylated fatty acid alkanolamides, and combinationsthereof.

When included therein the detergent will usually comprise from about0-10% by weight of a zwitterionic surfactant. Non-limiting examples ofzwitterionic surfactants include betaine, alkyldimethylbetaine,sulfobetaine, and combinations thereof.

Hydrotropes

A hydrotrope is a compound that solubilises hydrophobic compounds inaqueous solutions (or oppositely, polar substances in a non-polarenvironment). Typically, hydrotropes have both hydrophilic and ahydrophobic character (so-called amphiphilic properties as known fromsurfactants); however, the molecular structure of hydrotropes generallydo not favor spontaneous self-aggregation, see e.g. review by Hodgdonand Kaler (2007), Current Opinion in Colloid & Interface Science 12:121-128. Hydrotropes do not display a critical concentration above whichself-aggregation occurs as found for surfactants and lipids formingmiceller, lamellar or other well defined meso-phases. Instead, manyhydrotropes show a continuous-type aggregation process where the sizesof aggregates grow as concentration increases. However, many hydrotropesalter the phase behavior, stability, and colloidal properties of systemscontaining substances of polar and non-polar character, includingmixtures of water, oil, surfactants, and polymers. Hydrotropes areclassically used across industries from pharma, personal care, food, totechnical applications. Use of hydrotropes in detergent compositionsallow for example more concentrated formulations of surfactants (as inthe process of compacting liquid detergents by removing water) withoutinducing undesired phenomena such as phase separation or high viscosity.

The detergent may comprise 0-5% by weight, such as about 0.5-5%, orabout 3-5%, of a hydrotrope. Any hydrotrope known in the art for use indetergents may be utilized. Non-limiting examples of hydrotropes includesodium benzene sulfonate, sodium p-toluene sulfonate (STS), sodiumxylene sulfonate (SXS), sodium cumene sulfonate (SCS), sodium cymenesulfonate, amine oxides, alcohols and polyglycolethers, sodiumhydroxynaphthoate, sodium hydroxynaphthalene sulfonate, sodiumethylhexyl sulfate, and combinations thereof.

Builders and Co-Builders

The detergent composition may comprise about 0-65% by weight, such asabout 5-45% of a detergent builder or co-builder, or a mixture thereof.In a dish wash deteregent, the level of builder is typically 40-65%,particularly 50-65%. The builder and/or co-builder may particularly be achelating agent that forms water-soluble complexes with Ca and Mg. Anybuilder and/or co-builder known in the art for use in laundry detergentsmay be utilized. Non-limiting examples of builders include zeolites,diphosphates (pyrophosphates), triphosphates such as sodium triphosphate(STP or STPP), carbonates such as sodium carbonate, soluble silicatessuch as sodium metasilicate, layered silicates (e.g. SKS-6 fromHoechst), ethanolamines such as 2-aminoethan-1-ol (MEA), diethanolamine(DEA, also known as iminodiethanol), triethanolamine (TEA, also known as2,2′,2″-nitrilotriethanol), and carboxymethyl inulin (CMI), andcombinations thereof.

The detergent composition may also comprise 0-20% by weight, such asabout 5-10%, of a detergent co-builder, or a mixture thereof. Thedetergent composition may include include a co-builder alone, or incombination with a builder, for example a zeolite builder. Non-limitingexamples of co-builders include homopolymers of polyacrylates orcopolymers thereof, such as poly(acrylic acid)

(PAA) or copoly(acrylic acid/maleic acid) (PAA/PMA). Furthernon-limiting examples include citrate, chelators such asaminocarboxylates, aminopolycarboxylates and phosphonates, and alkyl- oralkenylsuccinic acid. Additional specific examples include2,2′,2″-nitrilotriacetic acid (NTA), ethylenediaminetetraacetic acid(EDTA), diethylenetriaminepentaacetic acid (DTPA), iminodisuccinic acid(IDS), ethylenediamine-N,M-disuccinic acid (EDDS), methylglycinediaceticacid (MGDA), glutamic acid-N,N-diacetic acid (GLDA),1-hydroxyethane-1,1-diphosphonic acid (HEDP),ethylenediaminetetra-(methylenephosphonic acid) (EDTMPA),diethylenetriaminepentakis(methylenephosphonic acid) (DTPMPA or DTMPA),N-(2-hydroxyethyl)iminodiacetic acid (EDG), aspartic acid-N-monoaceticacid (ASMA), aspartic acid-N,N-diacetic acid (ASDA), asparticacid-N-monopropionic acid (ASMP), iminodisuccinic acid (IDA),N-(2-sulfomethyl)-aspartic acid (SMAS), N-(2-sulfoethyl)-aspartic acid(SEAS), N-(2-sulfomethyl)-glutamic acid (SMGL),N-(2-sulfoethyl)-glutamic acid (SEGL), N-methyliminodiacetic acid(MIDA), α-alanine-N, N-diacetic acid (α-ALDA), serine-N, N-diacetic acid(SEDA), isoserine-N, N-diacetic acid (ISDA), phenylalanine-N, N-diaceticacid (PHDA), anthranilic acid-N, N-diacetic acid (ANDA), sulfanilicacid-N, N-diacetic acid (SLDA), taurine-N, N-diacetic acid (TUDA) andsulfomethyl-N, N-diacetic acid (SMDA),N-(2-hydroxyethyl)ethylidenediamine-N,N;N′-triacetate (HEDTA),diethanolglycine (DEG), diethylenetriamine penta(methylenephosphonicacid) (DTPMP), aminotris(methylenephosphonic acid) (ATMP), andcombinations and salts thereof. Further exemplary builders and/orco-builders are described in, e.g. WO09/102854, U.S. Pat. No. 5,977,053

Bleaching Systems: The detergent may comprise 0-50% by weight, such asabout 0.1-25%, of a bleaching system. Any bleaching system known in theart for use in laundry detergents may be utilized. Suitable bleachingsystem components include bleaching catalysts, photobleaches, bleachactivators, sources of hydrogen peroxide such as sodium percarbonate andsodium perborates, preformed peracids and mixtures thereof. Suitablepreformed peracids include, but are not limited to, peroxycarboxylicacids and salts, percarbonic acids and salts, perimidic acids and salts,peroxymonosulfuric acids and salts, for example, Oxone (R), and mixturesthereof. Non-limiting examples of bleaching systems includeperoxide-based bleaching systems, which may comprise, for example, aninorganic salt, including alkali metal salts such as sodium salts ofperborate (usually mono- or tetra-hydrate), percarbonate, persulfate,perphosphate, persilicate salts, in combination with a peracid-formingbleach activator. The term bleach activator is meant herein as acompound which reacts with peroxygen bleach like hydrogen peroxide toform a peracid. The peracid thus formed constitutes the activatedbleach. Suitable bleach activators to be used herein include thosebelonging to the class of esters amides, imides or anhydrides. Suitableexamples are tetracetylethylene diamine (TAED), sodium4-[(3,5,5-trimethylhexanoyl)oxy]benzene sulfonate (ISONOBS), diperoxydodecanoic acid, 4-(dodecanoyloxy)benzenesulfonate (LOBS),4-(decanoyloxy)benzenesulfonate, 4-(decanoyloxy)benzoate (DOBS),4-(nonanoyloxy)-benzenesulfonate (NOBS), and/or those disclosed inWO98/17767. A particular family of bleach activators of interest wasdisclosed in EP624154 and particulary preferred in that family is acetyltriethyl citrate (ATC). ATC or a short chain triglyceride like triacetinhas the advantage that it is environmental friendly as it eventuallydegrades into citric acid and alcohol. Furthermore acetyl triethylcitrate and triacetin has a good hydrolytical stability in the productupon storage and it is an efficient bleach activator. Finally ATCprovides a good building capacity to the laundry additive.Alternatively, the bleaching system may comprise peroxyacids of, forexample, the amide, imide, or sulfone type. The bleaching system mayalso comprise peracids such as 6-(phthalimido)peroxyhexanoic acid (PAP).The bleaching system may also include a bleach catalyst. In someembodiments the bleach component may be an organic catalyst selectedfrom the group consisting of organic catalysts having the followingformulae:

(iii) and mixtures thereof; wherein each R¹ is independently a branchedalkyl group containing from 9 to 24 carbons or linear alkyl groupcontaining from 11 to 24 carbons, preferably each R¹ is independently abranched alkyl group containing from 9 to 18 carbons or linear alkylgroup containing from 11 to 18 carbons, more preferably each R¹ isindependently selected from the group consisting of 2-propylheptyl,2-butyloctyl, 2-pentylnonyl, 2-hexyldecyl, n-dodecyl, n-tetradecyl,n-hexadecyl, n-octadecyl, iso-nonyl, iso-decyl, iso-tridecyl andiso-pentadecyl. Other exemplary bleaching systems are described, e.g. inWO02007/087258, WO2007/087244, WO2007/087259 and WO2007/087242. Suitablephotobleaches may for example be sulfonated zinc phthalocyanine.

Polymers: The detergent may comprise 0-10% by weight, such as 0.5-5%,2-5%, 0.5-2% or 0.2-1% of a polymer. Any polymer known in the art foruse in detergents may be utilized. The polymer may function as aco-builder as mentioned above, or may provide antiredeposition, fiberprotection, soil release, dye transfer inhibition, grease cleaningand/or anti-foaming properties. Some polymers may have more than one ofthe above-mentioned properties and/or more than one of thebelow-mentioned motifs. Exemplary polymers include(carboxymethyl)cellulose (CMC), poly(vinyl alcohol) (PVA),poly(vinylpyrrolidone) (PVP), poly(ethyleneglycol) or poly(ethyleneoxide) (PEG), ethoxylated poly(ethyleneimine), carboxymethyl inulin(CMI), and polycarboxylates such as PAA, PAA/PMA, poly-aspartic acid,and lauryl methacrylate/acrylic acid copolymers, hydrophobicallymodified CMC (HM-CMC) and silicones, copolymers of terephthalic acid andoligomeric glycols, copolymers of poly(ethylene terephthalate) andpoly(oxyethene terephthalate) (PET-POET), PVP, poly(vinylimidazole)(PVI), poly(vinylpyridine-N-oxide) (PVPO or PVPNO) andpolyvinylpyrrolidone-vinylimidazole (PVPVI). Further exemplary polymersinclude sulfonated polycarboxylates, polyethylene oxide andpolypropylene oxide (PEO-PPO) and diquaternium ethoxy sulfate. Otherexemplary polymers are disclosed in, e.g. WO 2006/130575. Salts of theabove-mentioned polymers are also contemplated.

Fabric hueing agents:The detergent compositions of the present inventionmay also comprise fabric hueing agents such as dyes or pigments, whichwhen formulated in detergent compositions can deposit onto a fabric whensaid fabric is contacted with a wash liquor comprising said detergentcompositions and thus altering the tint of said fabric throughabsorption/reflection of visible light. Fluorescent whitening agentsemit at least some visible light. In contrast, fabric hueing agentsalter the tint of a surface as they absorb at least a portion of thevisible light spectrum. Suitable fabric hueing agents include dyes anddye-clay conjugates, and may also include pigments. Suitable dyesinclude small molecule dyes and polymeric dyes. Suitable small moleculedyes include small molecule dyes selected from the group consisting ofdyes falling into the Colour Index (C.I.) classifications of DirectBlue, Direct Red, Direct Violet, Acid Blue, Acid Red, Acid Violet, BasicBlue, Basic Violet and Basic Red, or mixtures thereof, for example asdescribed in WO2005/03274, WO2005/03275, WO2005/03276 and EP1876226(hereby incorporated by reference). The detergent composition preferablycomprises from about 0.00003-0.2 wt %, from about 0.00008-0.05 wt %, oreven from about 0.0001-0.04 wt % fabric hueing agent. The compositionmay comprise from 0.0001-0.2 wt % fabric hueing agent, this may beespecially preferred when the composition is in the form of a unit dosepouch. Suitable hueing agents are also disclosed in, e.g. WO2007/087257and WO2007/087243.

Additional Enzymes

The detergent additive as well as the detergent composition may compriseone or more [additional] enzymes such as a protease, lipase, cutinase,an amylase, lichenase, carbohydrase, cellulase, pectinase, mannanase,arabinase, galactanase, xylanase, oxidase, e.g. a laccase, and/orperoxidase.

In general, the properties of the selected enzyme(s) should becompatible with the selected detergent, (i.e. pH-optimum, compatibilitywith other enzymatic and non-enzymatic ingredients, etc.), and theenzyme(s) should be present in effective amounts.

Cellulases

Suitable cellulases include those of bacterial or fungal origin.Chemically modified or protein engineered mutants are included. Suitablecellulases include cellulases from the genera Bacillus, Pseudomonas,Humicola, Fusarium, Thielavia, Acremonium, e.g. the fungal cellulasesproduced from Humicola insolens, Myceliophthora thermophila and Fusariumoxysporum disclosed in U.S. Pat. Nos. 4,435,307, 5,648,263, 5,691,178,5,776,757 and WO89/09259.

Especially suitable cellulases are the alkaline or neutral cellulaseshaving color care benefits. Examples of such cellulases are cellulasesdescribed in EP0495257, EP0531372, WO96/11262, WO96/29397, WO98/08940.Other examples are cellulase variants such as those described inWO94/07998, EP0531315, U.S. Pat. Nos. 5,457,046, 5,686,593, 5,763,254,WO95/24471, WO98/12307 and PCT/DK98/00299.

Example of cellulases exhibiting endo-beta-1,4-glucanase activity (EC3.2.1.4) are those having described in WO02/099091.

Other examples of cellulases include the family 45 cellulases describedin WO96/29397, and especially variants thereof having substitution,insertion and/or deletion at one or more of the positions correspondingto the following positions in SEQ ID NO:8 of WO02/099091: 2, 4, 7, 8,10, 13, 15, 19, 20, 21, 25, 26, 29, 32, 33, 34, 35, 37, 40, 42, 42a, 43,44, 48, 53, 54, 55, 58, 59, 63, 64, 65, 66, 67, 70, 72, 76, 79, 80, 82,84, 86, 88, 90, 91, 93, 95, 95d, 95h, 95j, 97, 100, 101, 102, 103, 113,114, 117, 119, 121, 133, 136, 137, 138, 139, 140a, 141, 143a, 145, 146,147, 150e, 150j, 151, 152, 153, 154, 155, 156, 157, 158, 159, 160c,160e, 160k, 161, 162, 164, 165, 168, 170, 171, 172, 173, 175, 176, 178,181, 183, 184, 185, 186, 188, 191, 192, 195, 196, 200, and/or 20,preferably selected among P19A, G20K, Q44K, N48E, Q119H or Q146 R.

Commercially available cellulases include Celluzyme™, and Carezyme™(Novozymes NS), Clazinase™, and Puradax HA™ (Genencor InternationalInc.), and KAC-500(B)™ (Kao Corporation).

Proteases

The additional enzyme may be another protease or protease variant. Theprotease may be of animal, vegetable or microbial origin, includingchemically or genetically modified mutants. Microbial origin ispreferred. It may be an alkaline protease, such as a serine protease ora metalloprotease. A serine protease may for example be of the S1family, such as trypsin, or the S8 family such as subtilisin. Ametalloproteases protease may for example be a thermolysin from e.g.family M4, M5, M7 or M8.

The term “subtilases” refers to a sub-group of serine protease accordingto Siezen et al., Protein Engng. 4 (1991) 719-737 and Siezen et al.Protein Science 6 (1997) 501-523. Serine proteases are a subgroup ofproteases characterized by having a serine in the active site, whichforms a covalent adduct with the substrate. The subtilases may bedivided into 6 sub-divisions, i.e. the Subtilisin family, the Thermitasefamily, the Proteinase K family, the Lantibiotic peptidase family, theKexin family and the Pyrolysin family. In one aspect of the inventionthe protease may be a subtilase, such as a subtilisin or a varianthereof. Further the subtilases (and the serine proteases) arecharacterised by having two active site amino acid residues apart fromthe serine, namely a histidine and an aspartic acid residue.

Examples of subtilisins are those derived from Bacillus such assubtilisin lentus, Bacillus lentus, subtilisin Novo, subtilisinCarlsberg, Bacillus licheniformis, subtilisin BPN′, subtilisin 309,subtilisin 147 and subtilisin 168 described in WO89/06279 and proteasePD138 (WO93/18140). Additional serine protease examples are described inWO98/020115, WO01/44452, WO01/58275, WO01/58276, WO03/006602 andWO04/099401. An example of a subtilase variants may be those havingmutations in any of the positions: 3, 4, 9, 15, 27, 36, 68, 76, 87, 95,96, 97, 98, 99, 100, 101, 102, 103, 104, 106, 118, 120, 123, 128, 129,130, 160, 167, 170, 194, 195, 199, 205, 217, 218, 222, 232, 235, 236,245, 248, 252 and 274 using the BPN' numbering. More preferred thesubtilase variants may comprise the mutations: S3T, V4I, S9R, A15T,K27R, *36D, V68A, N76D, N87S,R, *97E, A98S, S99G,D,A, S99AD, S101G,M,RS103A, V104I, Y,N, S106A, G118V,R, H120D,N, N123S, S128L, P129Q, S130A,G160D, Y167A, R170S, A194P, G195E, V199M, V205I, L217D, N218D, M222S,A232V, K235L, Q236H, Q245R, N252K, T274A (using BPN′ numbering). Afurther preferred protease is the alkaline protease from Bacillus lentusDSM 5483, as described for example in WO95/23221, and variants thereofwhich are described in WO92/21760, WO95/23221, EP1921147 and EP1921148.

Examples of trypsin-like proteases are trypsin (e.g. of porcine orbovine origin) and the Fusarium protease described in WO89/06270 andWO94/25583. Examples of useful proteases are the variants described inWO92/19729, WO98/20115, WO98/20116, and WO98/34946, especially thevariants with substitutions in one or more of the following positions:27, 36, 57, 76, 87, 97, 101, 104, 120, 123, 167, 170, 194, 206, 218,222, 224, 235, and 274.

Examples of metalloproteases are the neutral metalloprotease asdescribed in WO 07/044993.

Preferred commercially available protease enzymes include Alcalase™,Coronase™, Duralase™, Durazym™, Esperase™, Everlase™, Kannase™,Liquanase™, Liquanase Ultra™, Ovozyme™, Polarzyme™, Primase™, Relase™,Savinase™ and Savinase Ultra™, (Novozymes NS), Axapem™ (Gist-BrocasesN.V.), BLAP and BLAP X (Henkel AG & Co. KGaA), Excellase™, FN2™, FN3™,FN4™, Maxaca™, Maxapem™, Maxatase™, Properase™, Purafast™, Purafect™,Purafect OxP™, Purafect Prime™ and Puramax™ (Genencor int.).

Lipases and Cutinases

Suitable lipases and cutinases include those of bacterial or fungalorigin. Chemically modified or protein engineered mutant enzymes areincluded. Examples include lipase from Thermomyces, e.g. from T.lanuginosus (previously named Humicola lanuginosa) as described inEP258068 and EP305216, cutinase from Humicola, e.g. H. insolens(WO96/13580), lipase from strains of Pseudomonas (some of these nowrenamed to Burkholderia), e.g. P. alcaligenes or P. pseudoalcaligenes(EP218272), P. cepacia (EP331376), P. sp. strain SD705 (WO95/06720 &WO96/27002), P. wisconsinensis (WO96/12012), GDSL-type Streptomyceslipases (WO10/065455), cutinase from Magnaporthe grisea (WO10/107560),cutinase from Pseudomonas mendocina (U.S. Pat. No. 5,389,536), lipasefrom Thermobifida fusca (WO11/084412), Geobacillus stearothermophiluslipase (WO11/084417), lipase from Bacillus subtilis (WO11/084599), andlipase from Streptomyces griseus (WO11/150157) and S. pristinaespiralis(WO12/137147).

Further examples are lipases sometimes referred to as acyltransferasesor perhydrolases, e.g. acyltransferases with homology to Candidaantarctica lipase A (WO10/111143), acyltransferase from Mycobacteriumsmegmatis (WO05/56782), perhydrolases from the CE 7 family (WO09/67279),and variants of the M. smegmatis perhydrolase in particular the S54Vvariant used in the commercial product Gentle Power Bleach from HuntsmanTextile Effects Pte Ltd (WO10/100028).

Other examples are lipase variants such as those described in EP407225,WO92/05249, WO94/01541, WO94/25578, WO95/14783, WO95/30744, WO95/35381,WO95/22615, WO96/00292, WO97/04079, WO97/07202, WO00/34450, WO00/60063,WO01/92502, WO07/87508 and WO09/109500.

Preferred commercial lipase products include include Lipolase™, Lipex™;Lipolex™ and Lipoclean™ (Novozymes NS), Lumafast (originally fromGenencor) and Lipomax (originally from Gist-Brocades).

Amylases

The amylase may be an alpha-amylase, a beta-amylase or a glucoamylaseand may be of bacterial or fungal origin. Chemically modified or proteinengineered mutants are included. Amylases include, for example,alpha-amylases obtained from Bacillus, e.g. a special strain of Bacilluslicheniformis, described in more detail in GB1296839.

Examples of amylases are those having SEQ ID NO:3 in WO95/10603 orvariants having 90% sequence identity to SEQ ID NO:3 thereof. Preferredvariants are described in WO94/02597, WO94/18314, WO97/43424 and SEQ IDNO:4 of WO99/019467, such as variants with substitutions in one or moreof the following positions: 15, 23, 105, 106, 124, 128, 133, 154, 156,178, 179, 181, 188, 190, 197, 201, 202, 207, 208, 209, 211, 243, 264,304, 305, 391, 408, and 444 of SEQ ID NO:3 in WO95/10603.

Further amylases which can be used are amylases having SEQ ID NO:6 inWO02/010355 or variants thereof having 90% sequence identity to SEQ IDNO:6. Preferred variants of SEQ ID NO:6 are those having a deletion inpositions 181 and 182 and a substitution in position 193.

Other amylase examples are hybrid alpha-amylase comprising residues 1-33of the alpha-amylase derived from B. amyloliquefaciens shown in SEQ IDNO:6 of WO2006/066594 and residues 36-483 of the B. licheniformisalpha-amylase shown in SEQ ID NO:4 of WO2006/066594 or variants having90% sequence identity thereof. Preferred variants of this hybridalpha-amylase are those having a substitution, a deletion or aninsertion in one of more of the following positions: G48, T49, G107,H156, A181, N190, M197, I201, A209 and Q264. Most preferred variants ofthe hybrid alpha-amylase comprising residues 1-33 of the alpha-amylasederived from B. amyloliquefaciens shown in SEQ ID NO:6 of WO2006/066594and residues 36-483 of SEQ ID NO:4 are those having the substitutions:M197T; H156Y+A181T+N190F+A209V+Q264S; orG48+T49+G107+H156+A181+N190+I201+A209+Q264.

Further amylase examples are amylases having SEQ ID NO:6 in WO99/019467or variants thereof having 90% sequence identity to SEQ ID NO:6.Preferred variants of SEQ ID NO:6 are those having a substitution, adeletion or an insertion in one or more of the following positions:R181, G182, H183, G184, N195, I206, E212, E216 and K269. Particularlypreferred amylases are those having deletion in positions G182 and H183or positions H183 and G184.

Additional amylases are those having SEQ ID NO:1, SEQ ID NO:2 or SEQ IDNO:7 of WO96/023873 or variants thereof having 90% sequence identity toSEQ ID NO:1, SEQ ID NO:2 or SEQ ID NO:7. Preferred variants of SEQ IDNO:1, SEQ ID NO:2 or SEQ ID NO:7 are those having a substitution, adeletion or an insertion in one or more of the following positions: 140,181, 182, 183, 184, 195, 206, 212, 243, 260, 269, 304 and 476. Morepreferred variants are those having a deletion in positions 182 and 183or positions 183 and 184. Most preferred amylase variants of SEQ IDNO:1, SEQ ID NO:2 or SEQ ID NO:7 are those having a deletion inpositions 183 and 184 and a substitution in positions 140, 195, 206,243, 260, 304 and 476.

Other amylases which can be used are amylases having SEQ ID NO:2 ofWO08/153815, SEQ ID NO:10 in WO01/66712 or variants thereof having 90%sequence identity to SEQ ID NO:2 of WO08/153815 or 90% sequence identityto SEQ ID NO:10 in WO 01/66712. Preferred variants of SEQ ID NO:10 inWO01/66712 are those having a substitution, a deletion or an insertionin one of more of the following positions: 176, 177, 178, 179, 190, 201,207, 211 and 264.

Further amylases which can be used are amylases having SEQ ID NO:2 ofWO09/061380 or variants thereof having 90% sequence identity to SEQ IDNO:2. Preferred variants of SEQ ID NO:2 are those having a substitution,a deletion or an insertion in one of more of the following positions:Q87, Q98, S125, N128, T131, T165, K178, R180, S181, T182, G183, M201,F202, N225, S243, N272, N282, Y305, R309, D319, Q320, Q359, K444 andG475. More preferred variants of SEQ ID NO:2 are those having thesubstitution in one of more of the following positions: Q87E,R, Q98R,S125A, N128C, T131I, T165I, K178L, T182G, M201L, F202Y, N225E,R,N272E,R, S243Q,A,E,D, Y305R, R309A, Q320R, Q359E, K444E and G475K and/ordeletion in position R180 and/or S181. Most preferred amylase variantsof SEQ ID NO:2 are those having the substitutions:N128C+K178L+T182G+Y305R+G475K;N128C+K178L+T182G+F202Y+Y305R+D319T+G475K;S125A+N128C+K178L+T182G+Y305R+G475K; orS125A+N128C+T1311+T1651+K178L+T182G+Y305R+G475K wherein the variantoptionally further comprises a substitution at position 243 and/or adeletion at position 180 and/or position 181.

Other amylases are variants of SEQ ID NO:1 of WO2016/203064 having atleast 75% sequence identity to SEQ ID NO:1 thereof. Preferred variantsare variants comprising a modification in one or more positionscorresponding to positions 1, 54, 56, 72, 109, 113, 116, 134, 140, 159,167, 169, 172, 173, 174, 181, 182, 183, 184, 189, 194, 195, 206, 255,260, 262, 265, 284, 289, 304, 305, 347, 391, 395, 439, 469, 444, 473,476, or 477 of SEQ ID NO:1, wherein said alpha-amylase variant has asequence identity of at least 75% but less than 100% to SEQ ID NO:1.

Other examples of amylases are the alpha-amylase having SEQ ID NO:12 inWO01/66712 or a variant having at least 90%, such as at least 95%,sequence identity to SEQ ID NO:12. Preferred amylase variants are thosehaving a substitution, a deletion or an insertion in one of more of thefollowing positions of SEQ ID NO:12 in WO01/66712: R28, R118, N174;R181, G182, D183, G184, G186, W189, N195, M202, Y298, N299, K302, S303,N306, R310, N314; R320, H324, E345, Y396, R400, W439, R444, N445, K446,Q449, R458, N471, N484. Particular preferred amylases include variantshaving a deletion of D183 and G184 and having the substitutions R118K,N195F, R320K and R458K, and a variant additionally having substitutionsin one or more position selected from the group: M9, G149, G182, G186,M202, T257, Y295, N299, M323, E345 and A339, most preferred a variantthat additionally has substitutions in all these positions.

Commercially available amylases are Duramyl™, Termamyl™, Fungamyl™,Stainzyme™, Stainzyme Plus™, Natalase™ and BAN™ (Novozymes NS),Rapidase™ and Purastar™ (from Genencor International Inc.).

Peroxidases/Oxidases: Suitable peroxidases/oxidases include those ofplant, bacterial or fungal origin. Chemically modified or proteinengineered mutants are included. Examples of useful peroxidases includeperoxidases from Coprinus, e.g. from C. cinereus, and variants thereofas those described in WO93/24618, WO95/10602, and WO98/15257.Commercially available peroxidases include Guardzyme™ (Novozymes NS).

The detergent enzyme(s) may be included in a detergent composition byadding separate additives containing one or more enzymes, or by adding acombined additive comprising all of these enzymes. A detergent additiveof the invention, i.e. a separate additive or a combined additive, canbe formulated, for example, as a granulate, liquid, slurry, etc.Preferred detergent additive formulations are granulates, in particularnon-dusting granulates, liquids, in particular stabilized liquids, orslurries.

Non-dusting granulates may be produced, e.g. as disclosed in U.S. Pat.Nos. 4,106,991 and 4,661,452 and may optionally be coated by methodsknown in the art. Examples of waxy coating materials are poly(ethyleneoxide) products (polyethyleneglycol, PEG) with mean molar weights of1000 to 20000;

ethoxylated nonylphenols having from 16 to 50 ethylene oxide units;ethoxylated fatty alcohols in which the alcohol contains from 12 to 20carbon atoms and in which there are 15 to 80 ethylene oxide units; fattyalcohols; fatty acids; and mono- and di- and triglycerides of fattyacids. Examples of film-forming coating materials suitable forapplication by fluid bed techniques are given in GB1483591. Liquidenzyme preparations may, for instance, be stabilized by adding a polyolsuch as propylene glycol, a sugar or sugar alcohol, lactic acid or boricacid according to established methods. Protected enzymes may be preparedaccording to the method disclosed in EP238216.

Adjunct materials: Any detergent components known in the art for use inlaundry detergents may also be utilized. Other optional detergentcomponents include anti-corrosion agents, anti-shrink agents, anti-soilredeposition agents, anti-wrinkling agents, bactericides, binders,corrosion inhibitors, disintegrants/disintegration agents, dyes, enzymestabilizers (including boric acid, borates, CMC, and/or polyols such aspropylene glycol), fabric conditioners including clays,fillers/processing aids, fluorescent whitening agents/opticalbrighteners, foam boosters, foam (suds) regulators, perfumes,soil-suspending agents, softeners, suds suppressors, tarnish inhibitors,and wicking agents, either alone or in combination. Any ingredient knownin the art for use in laundry detergents may be utilized. The choice ofsuch ingredients is well within the skill of the artisan.

Dispersants: The detergent compositions of the present invention canalso contain dispersants. In particular, powdered detergents maycomprise dispersants. Suitable water-soluble organic materials includethe homo- or co-polymeric acids or their salts, in which thepolycarboxylic acid comprises at least two carboxyl radicals separatedfrom each other by not more than two carbon atoms. Suitable dispersantsare for example described in Powdered Detergents, Surfactant scienceseries volume 71, Marcel Dekker, Inc.

Dye Transfer Inhibiting Agents: The detergent compositions of thepresent invention may also include one or more dye transfer inhibitingagents. Suitable polymeric dye transfer inhibiting agents include, butare not limited to, polyvinylpyrrolidone polymers, polyamine N-oxidepolymers, copolymers of N-vinylpyrrolidone and N-vinylimidazole,polyvinyloxazolidones and polyvinylimidazoles or mixtures thereof. Whenpresent in a subject composition, the dye transfer inhibiting agents maybe present at levels from about 0.0001-10%, from about 0.01-5% or evenfrom about 0.1-3% by weight of the composition.

Fluorescent whitening agent: The detergent compositions of the presentinvention will preferably also contain additional components that maytint articles being cleaned, such as fluorescent whitening agent oroptical brighteners. Where present the brightener is preferably at alevel of about 0,01-0,5%. Any fluorescent whitening agent suitable foruse in a laundry detergent composition may be used in the composition ofthe present invention. The most commonly used fluorescent whiteningagents are those belonging to the classes of diaminostilbene-sulphonicacid derivatives, diarylpyrazoline derivatives and bisphenyl-distyrylderivatives. Examples of the diaminostilbene-sulphonic acid derivativetype of fluorescent whitening agents include the sodium salts of:4,4′-bis-(2-diethanolamino-4-anilino-s-triazin-6-ylamino)stilbene-2,2′-disulphonate; 4,4′-bis-(2,4-dianilino-s-triazin-6-ylamino)stilbene-2.2′-disulphonate;4,4′-bis-(2-anilino-4(N-methyl-N-2-hydroxy-ethylamino)-s-triazin-6-ylamino)stilbene-2,2′-disulphonate,4,4′-bis-(4-phenyl-2,1,3-triazol-2-yl)stilbene-2,2′-disulphonate;4,4′-bis-(2-anilino-4(1-methyl-2-hydroxy-ethylamino)-s-triazin-6-ylamino)stilbene-2,2′-disulphonate and2-(stilbyl-4″-naptho-1.,2′:4,5)-1,2,3-trizole-2″-sulphonate. Preferredfluorescent whitening agents are Tinopal DMS and Tinopal CBS availablefrom Ciba-Geigy AG, Basel, Switzerland. Tinopal DMS is the disodium saltof 4,4′-bis-(2-morpholino-4 anilino-s-triazin-6-ylamino) stilbenedisulphonate. Tinopal CBS is the disodium salt of2,2′-bis-(phenyl-styryl) disulphonate. Also preferred are fluorescentwhitening agents is the commercially available Parawhite KX, supplied byParamount Minerals and Chemicals, Mumbai, India. Other fluorescerssuitable for use in the invention include the 1-3-diaryl pyrazolines andthe 7-alkylaminocoumarins. Suitable fluorescent brightener levelsinclude lower levels of from about 0.01, from 0.05, from about 0.1 oreven from about 0.2 wt.% to upper levels of 0.5 or even 0.75 wt %.

Soil release polymers: The detergent compositions of the presentinvention may also include one or more soil release polymers which aidthe removal of soils from fabrics such as cotton and polyester basedfabrics, in particular the removal of hydrophobic soils from polyesterbased fabrics. The soil release polymers may for example be nonionic oranionic terephthalte based polymers, polyvinyl caprolactam and relatedcopolymers, vinyl graft copolymers, polyester polyamides see for exampleChapter 7 in Powdered Detergents, Surfactant science series volume 71,Marcel Dekker, Inc. Another type of soil release polymers is amphiphilicalkoxylated grease cleaning polymers comprising a core structure and aplurality of alkoxylate groups attached to that core structure. The corestructure may comprise a polyalkylenimine structure or apolyalkanolamine structure as described in detail in WO2009/087523(hereby incorporated by reference). Furthermore random graft co-polymersare suitable soil release polymers Suitable graft co-polymers aredescribed in more detail in WO2007/138054, WO 2006/108856 andWO2006/113314 (hereby incorporated by reference). Other soil releasepolymers are substituted polysaccharide structures especiallysubstituted cellulosic structures such as modified cellulosederiviatives such as those described in EP1867808 or WO2003/040279 (bothare hereby incorporated by reference). Suitable cellulosic polymersinclude cellulose, cellulose ethers, cellulose esters, cellulose amidesand mixtures thereof. Suitable cellulosic polymers include anionicallymodified cellulose, nonionically modified cellulose, cationicallymodified cellulose, zwitterionically modified cellulose, and mixturesthereof. Suitable cellulosic polymers include methyl cellulose, carboxymethyl cellulose, ethyl cellulose, hydroxyl ethyl cellulose, hydroxylpropyl methyl cellulose, ester carboxy methyl cellulose, and mixturesthereof.

Anti-redeposition agents: The detergent compositions of the presentinvention may also include one or more anti-redeposition agents such ascarboxymethylcellulose (CMC), polyvinyl alcohol (PVA),polyvinylpyrrolidone (PVP), polyoxyethylene and/or polyethyleneglycol(PEG), homopolymers of acrylic acid, copolymers of acrylic acid andmaleic acid, and ethoxylated polyethyleneimines. The cellulose basedpolymers described under soil release polymers above may also functionas anti-redeposition agents.

Other suitable adjunct materials include, but are not limited to,anti-shrink agents, anti-wrinkling agents, bactericides, binders,carriers, dyes, enzyme stabilizers, fabric softeners, fillers, foamregulators, hydrotropes, perfumes, pigments, sod suppressors, solvents,and structurants for liquid detergents and/or structure elasticizingagents.

Formulation of Detergent Products

The detergent composition may be in any convenient form, e.g. a bar, ahomogenous tablet, a tablet having two or more layers, a pouch havingone or more compartments, a regular or compact powder, a granule, apaste, a gel, or a regular, compact or concentrated liquid. There are anumber of detergent formulation forms such as layers (same or differentphases), pouches, as well as forms for machine dosing unit.

Pouches can be configured as single or multicompartments. It can be ofany form, shape and material which is suitable for hold the composition,e.g. without allowing the release of the composition from the pouchprior to water contact. The pouch is made from water soluble film whichencloses an inner volume. Said inner volume can be devided intocompartments of the pouch. Preferred films are polymeric materialspreferably polymers which are formed into a film or sheet. Preferredpolymers, copolymers or derivates therof are selected polyacrylates, andwater soluble acrylate copolymers, methyl cellulose, carboxy methylcellulose, sodium dextrin, ethyl cellulose, hydroxyethyl cellulose,hydroxypropyl methyl cellulose, malto dextrin, poly methacrylates, mostpreferably polyvinyl alcohol copolymers and, hydroxyprpyl methylcellulose (HPMC). Preferably the level of polymer in the film forexample PVA is at least about 60%. Preferred average molecular weightwill typically be about 20,000 to about 150,000. Films can also be ofblend compositions comprising hydrolytically degradable and watersoluble polymer blends such as polyactide and polyvinyl alcohol (knownunder the Trade reference M8630 as sold by Chris Craft In. Prod. OfGary, Ind., US) plus plasticisers like glycerol, ethylene glycerol,Propylene glycol, sorbitol and mixtures thereof. The pouches cancomprise a solid laundry cleaning composition or part components and/ora liquid cleaning composition or part components separated by the watersoluble film. The compartment for liquid components can be different incomposition than compartments containing solids. Ref:(US2009/0011970A1).

Detergent ingredients can be separated physically from each other bycompartments in water dissolvable pouches or in different layers oftablets. Thereby negative storage interaction between components can beavoided. Different dissolution profiles of each of the compartments canalso give rise to delayed dissolution of selected components in the washsolution.

A liquid or gel detergent, which is not unit dosed, may be aqueous,typically containing at least 20% by weight and up to 95% water, such asup to about 70% water, up to about 65% water, up to about 55% water, upto about 45% water, up to about 35% water. Other types of liquids,including without limitation, alkanols, amines, diols, ethers andpolyols may be included in an aqueous liquid or gel. An aqueous liquidor gel detergent may contain from 0-30% organic solvent. A liquid or geldetergent may be non-aqueous.

Laundry Soap Bars

The enzymes of the invention may be added to laundry soap bars and usedfor hand washing laundry, fabrics and/or textiles. The term laundry soapbar includes laundry bars, soap bars, combo bars, syndet bars anddetergent bars. The types of bar usually differ in the type ofsurfactant they contain, and the term laundry soap bar includes thosecontaining soaps from fatty acids and/or synthetic soaps. The laundrysoap bar has a physical form which is solid and not a liquid, gel or apowder at room temperature. The term solid is defined as a physical formwhich does not significantly change over time, i.e. if a solid object(e.g. laundry soap bar) is placed inside a container, the solid objectdoes not change to fill the container it is placed in. The bar is asolid typically in bar form but can be in other solid shapes such asround or oval.

The laundry soap bar may contain one or more additional enzymes,protease inhibitors such as peptide aldehydes (or hydrosulfite adduct orhemiacetal adduct), boric acid, borate, borax and/or phenylboronic acidderivatives such as 4-formylphenylboronic acid, one or more soaps orsynthetic surfactants, polyols such as glycerine, pH controllingcompounds such as fatty acids, citric acid, acetic acid and/or formicacid, and/or a salt of a monovalent cation and an organic anion whereinthe monovalent cation may be for example Na⁺, K⁺ or NH₄ ⁺ and theorganic anion may be for example formate, acetate, citrate or lactatesuch that the salt of a monovalent cation and an organic anion may be,for example, sodium formate.

The laundry soap bar may also contain complexing agents like EDTA andHEDP, perfumes and/or different type of fillers, surfactants e.g.anionic synthetic surfactants, builders, polymeric soil release agents,detergent chelators, stabilizing agents, fillers, dyes, colorants, dyetransfer inhibitors, alkoxylated polycarbonates, suds suppressers,structurants, binders, leaching agents, bleaching activators, clay soilremoval agents, anti-redeposition agents, polymeric dispersing agents,brighteners, fabric softeners, perfumes and/or other compounds known inthe art.

The laundry soap bar may be processed in conventional laundry soap barmaking equipment such as but not limited to: mixers, plodders, e.g a twostage vacuum plodder, extruders, cutters, logo-stampers, cooling tunnelsand wrappers. The invention is not limited to preparing the laundry soapbars by any single method. The premix of the invention may be added tothe soap at different stages of the process. For example, the premixcontaining a soap, an enzyme, optionally one or more additional enzymes,a protease inhibitor, and a salt of a monovalent cation and an organicanion may be prepared and and the mixture is then plodded. The enzymeand optional additional enzymes may be added at the same time as theprotease inhibitor for example in liquid form. Besides the mixing stepand the plodding step, the process may further comprise the steps ofmilling, extruding, cutting, stamping, cooling and/or wrapping.

Method of Producing the Composition

The present invention also relates to methods of producing thecomposition. The method may be relevant for the (storage) stability ofthe detergent composition: e.g. Soap bar premix method WO2009155557.

Uses

The present invention is also directed to methods for using thedetergent compositions thereof. The present invention may be used forexample in any detergent application which requries the degradation ofxanthan gum.

Use to Degrade Xanthan Gum

Xanthan gum has been used as an ingredient in many consumer productsincluding foods and cosmetics and has found use in the oil industry.Therefore, the degradation of xanthan gum can result in improvedcleaning processes, such as the easier removal of stains containinggums, such as xanthan gum. Thus, the present invention is directed tothe use of detergent compositions comprising GH9 endoglucanases (e.g.variants described herein) of the invention to degrade xanthan gum. Thepresent invention is also directed to the use of xanthan lyases in thecompositions of the invention to degrade xanthan gum. An embodiment isthe use of a detergent composition comprising GH9 endoglucanases asdescribed herein (e.g. variants) together with xanthan lyases to degradexanthan gum. Degradation of xanthan gum can preferably be measured usingthe viscosity reduction assay (e.g. ViPr assay) or alternatively asdescribred in Examples 3 and 7 herein.

GH9 endoglucanase activity may alternatively be measured by assessmentof reducing ends on xanthan gum pre-treated with xanthan lyase using thecolorimetric assay developed by Lever (1972), Anal. Biochem. 47:273-279, 1972. A preferred embodiment is the use of 0.1% xanthan gumpre-treated with xanthan lyase. Degradation of xanthan gum pre-treatedwith xanthan lyase may be determined by calculating difference betweenblank and sample, wherein a difference of more than 0.5 mAU, preferablymore than 0.6 mAU, more preferably more than 0.7 mAU or even morepreferably more than 0.8 mAU, shows degradation of xanthan gumpre-treated with xanthan lyase.

Xanthan lyase activity may alternatively be measured by assessment ofreducing ends liberated from xanthan gum using the colorimetric assaydeveloped by Lever (1972), Anal. Biochem. 47: 273-279, 1972. A preferredembodiment is the use of 0.1% xanthan gum. Degradation of xanthan gummay be determined by calculating difference between blank and samplewherein a difference of more than 0.1 mAU, preferably more than 0.15mAU, more preferably more than 0.2 mAU or even more preferably more than0.25 mAU shows degradation of xanthan gum.

GH9 endoglucanase and xanthan lyase activity may alternatively bemeasured by assessment of reducing ends liberated from xanthan gum usingthe colorimetric assay developed by Lever (1972), Anal. Biochem. 47:273-279, 1972. A preferred embodiment is the use of 0.1% xanthan gum.Degradation of xanthan gum may be determined by calculating differencebetween blank and sample wherein a difference of more than 0.4 mAU,preferably more than 0.5 mAU, more preferably more than 0.6 mAU or evenmore preferably more than 0.8 mAU shows degradation of xanthan gum.

The invention also relates to methods for degrading xanthan gumcomprising applying a detergent composition comprising one or more GH9endoglucanases described herein (e.g. variants) and one or more xanthanlyases described herein (e.g. variants) to xanthan gum.

Use in Detergents

The present invention inter alia relates to the use of detergentcompositions comprising GH9 endoglucanases and xanthan lyases asdescribed herein (e.g. variants) in cleaning processes such as thelaundering of textiles and fabrics (e.g. household laundry washing andindustrial laundry washing), as well as household and industrial hardsurface cleaning, such as dish wash. For this, the GH9 endoglucanasesand xanthan lyases (e.g. variants) may be added to a detergentcomposition comprising of one or more detergent components.

The polypeptides described herein (e.g. variants) may be added to andthus become a component of a detergent composition. The detergentcomposition may be formulated, for example, as a hand or machine laundrydetergent composition for both household and industrial laundrycleaning, including a laundry additive composition suitable forpre-treatment of stained fabrics and a rinse added fabric softenercomposition, or be formulated as a detergent composition for use ingeneral household or industrial hard surface cleaning operations, or beformulated for hand or machine (both household and industrial)dishwashing operations. In a specific aspect, the present inventionrelates to a detergent additive comprising a polypeptide as describedherein.

The invention also relates to methods for degrading xanthan gum on thesurface of a textile or hard surface, such as dish wash, comprisingapplying a detergent composition as described herein to xanthan gum.

It has been contemplated that the use of a GH9 endoglucanase and xanthanlyase as described herein (e.g. a variant as described herein) alonegives an enzyme detergency benefit, preferably an enzyme detergencybenefit on xanthan gum.

In some aspects, the invention relates to the use of a detergentcomposition comprising one or more detergent components and an isolatedGH9 endoglucanase described herein (e.g. a variant) together with anisolated xanthan lyase described herein (e.g. variant).

The invention is further defined in the following paragraphs:

1. A detergent composition comprising

(A) an endoglucanase variant, comprising an alteration (e.g. asubstitution, deletion or insertion, preferably a substitution) at oneor more positions in a region selected from the group consisting of:

i) region 1 corresponding to amino acids 95 to 105 of SEQ ID NO:2, e.g.said alteration at one or more positions selected from the groupconsisting of positions: 95, 96, 97, 98, 99, 100, 101, 102, 103, 104,105, wherein said positions correspond to amino acid positions of SEQ IDNO:2 (e.g. using the numbering of SEQ ID NO:2),

ii) region 2 corresponding to amino acids 115 to 138 of SEQ ID NO:2,e.g. said alteration at one or more positions selected from the groupconsisting of positions: 115, 116, 117, 118, 119, 120, 121, 122, 123,124, 125, 126, 127, 128, 129, 130, 131, 132, 133, 134, 135, 136, 137,138, wherein said positions correspond to amino acid positions of SEQ IDNO:2 (e.g. using the numbering of SEQ ID NO:2),

iii) region 3 corresponding to amino acids 210 to 251 of SEQ ID NO:2,e.g. said alteration at one or more positions selected from the groupconsisting of positions: 210, 211, 212, 213, 214, 215, 216, 217, 218,219, 220, 221, 222, 223, 224, 225, 226, 227, 228, 229, 230, 231, 232,233, 234, 235, 236, 237, 238, 239, 240, 241, 242, 243, 244, 245, 246,247, 248, 249, 250, 251, wherein said positions correspond to amino acidpositions of SEQ ID NO:2 (e.g. using the numbering of SEQ ID NO:2),

iv) region 4 corresponding to amino acids 267 to 301 of SEQ ID NO:2,e.g. said alteration at one or more positions selected from the groupconsisting of positions: 267, 268, 269, 270, 271, 272, 273, 274, 275,276, 277, 278, 279, 280, 281, 282, 283, 284, 285, 286, 287, 288, 289,290, 291, 292, 293, 294, 295, 296, 297, 298, 299, 300, 301, wherein saidpositions correspond to amino acid positions of SEQ ID NO:2 (e.g. usingthe numbering of SEQ ID NO:2),

v) region 5 corresponding to amino acids 339 to 361 of SEQ ID NO:2, e.g.said alteration at one or more positions selected from the groupconsisting of positions: 339, 340, 341, 342, 343, 344, 345, 346, 347,348, 349, 350, 351, 352, 353, 354, 355, 356, 357, 358, 359, 360, 361,wherein said positions correspond to amino acid positions of SEQ ID NO:2(e.g. using the numbering of SEQ ID NO:2),

vi) region 6 corresponding to amino acids 547 to 595 of SEQ ID NO:2,e.g. said alteration at one or more positions selected from the groupconsisting of positions: 547, 548, 549, 550, 551, 552, 553, 554, 555,556, 557, 558, 559, 560, 561, 562, 563, 564, 565, 566, 567, 568, 569,570, 571, 572, 573, 574, 575, 576, 577, 578, 579, 580, 581, 582, 583,584, 585, 586, 587, 588, 589, 590, 591, 592, 593, 594, 595, wherein saidpositions correspond to amino acid positions of SEQ ID NO:2 (e.g. usingthe numbering of SEQ ID NO:2),

vii) region 7 corresponding to amino acids 612 to 660 of SEQ ID NO:2,e.g. said alteration at one or more positions selected from the groupconsisting of positions: 612, 613, 614, 615, 616, 617, 618, 619, 620,621, 622, 623, 624, 625, 626, 627, 628, 629, 630, 631, 632, 633, 634,635, 636, 637, 638, 639, 640, 641, 642, 643, 644, 645, 646, 647, 648,649, 650, 651, 652, 653, 654, 655, 656, 657, 658, 659, 660, wherein saidpositions correspond to amino acid positions of SEQ ID NO:2 (e.g. usingthe numbering of SEQ ID NO:2),

viii) region 8 corresponding to amino acids 806 to 828 of SEQ ID NO:2,e.g. said alteration at one or more positions selected from the groupconsisting of positions: 806, 807, 808, 809, 810, 811, 812, 813, 814,815, 816, 817, 818, 819, 820, 821, 822, 823, 824, 825, 826, 827, 828,wherein said positions correspond to amino acid positions of SEQ ID NO:2(e.g. using the numbering of SEQ ID NO:2), and

ix) region 9 corresponding to amino acids 839 to 1042 of SEQ ID NO:2,e.g. said alteration at one or more positions selected from the groupconsisting of positions: 839, 840, 841, 842, 843, 844, 845, 846, 847,848, 849, 850, 851, 852, 853, 854, 855, 856, 857, 858, 859, 860, 861,862, 863, 864, 865, 866, 867, 868, 869, 870, 871, 872, 873, 874, 875,876, 877, 878, 879, 880, 881, 882, 883, 884, 885, 886, 887, 888, 889,890, 891, 892, 893, 894, 895, 896, 897, 898, 899, 900, 901, 902, 903,904, 905, 906, 907, 908, 909, 910, 911, 912, 913, 914, 915, 916, 917,918, 919, 920, 921, 922, 923, 924, 925, 926, 927, 928, 929, 930, 931,932, 933, 934, 935, 936, 937, 938, 939, 940, 941, 942, 943, 944, 945,946, 947, 948, 949, 950, 951, 952, 953, 954, 955, 956, 957, 958, 959,960, 961, 962, 963, 964, 965, 966, 967, 968, 969, 970, 971, 972, 973,974, 975, 976, 977, 978, 979, 980, 981, 982, 983, 984, 985, 986, 987,988, 989, 990, 991, 992, 993, 994, 995, 996, 997, 998, 999, 1000, 1001,1002, 1003, 1004, 1005, 1006, 1007, 1008, 1009, 1010, 1011, 1012, 1013,1014, 1015, 1016, 1017, 1018, 1019, 1020, 1021, 1022, 1023, 1024, 1025,1026, 1027, 1028, 1029, 1030, 1031, 1032, 1033, 1034, 1035, 1036, 1037,1038, 1039, 1040, 1041, 1042, wherein said positions correspond to aminoacid positions of SEQ ID NO:2 (e.g. using the numbering of SEQ ID NO:2),

wherein said variant has at least 60%, e.g. at least 65%, 70%, 75%, 80%,85%, 90%, 95%, 96%, 97%, 98%, or 99%, and less than 100% sequenceidentity to SEQ ID NO:2; preferably said endoglucanase variant hasactivity on xanthan gum pretreated with xanthan lyase, furtherpreferably said activity is a xanthan gum degrading activity; and

(B) a xanthan lyase variant, comprising an alteration (e.g. asubstitution, deletion or insertion, preferably a substitution) at oneor more positions in a region selected from the group consisting of: (i)region 1 corresponding to amino acids 154 to 176 of SEQ ID NO:6; (ii)region 2 corresponding to amino acids 614 to 658 of SEQ ID NO:6; (iii)region 3 corresponding to amino acids 731 to 803 of SEQ ID NO:6; (iv)region 4 corresponding to amino acids 807 to 846 of SEQ ID NO:6; (v)region 5 corresponding to amino acids 872 to 885 of SEQ ID NO:6; (vi)region 6 corresponding to amino acids 903 to 1004 of SEQ ID NO:6; (vii)region 7 corresponding to amino acids 1 to 153 of SEQ ID NO:6; (viii)region 8 corresponding to amino acids 177 to 613 of SEQ ID NO:6; (ix)region 9 corresponding to amino acids 659 to 730 of SEQ ID NO:6; (x)region 10 corresponding to amino acids 804 to 806 of SEQ ID NO:6; (xi)region 11 corresponding to amino acids 847 to 871 of SEQ ID NO:6; (xii)region 12 corresponding to amino acids 886 to 902 of SEQ ID NO:6; (xiii)region 13 corresponding to amino acids 1005 to 1037 of SEQ ID NO:6;wherein said variant has at least 60%, e.g. at least 65%, 70%, 75%, 80%,85%, 90%, 95%, 96%, 97%, 98%, or 99%, and less than 100% sequenceidentity to SEQ ID NO:6; preferably said xanthan lyase variant hasactivity on xanthan gum, further preferably said activity is a xanthangum degrading activity.

2. The detergent composition comprising an endoglucanase variant ofparagraph 1, which is a variant of a parent endoglucanase selected fromthe group consisting of: (a) a polypeptide having at least 60% sequenceidentity to the mature polypeptide of SEQ ID NO:2; (b) a polypeptideencoded by a polynucleotide that hybridizes under low stringencyconditions with (i) the mature polypeptide coding sequence of SEQ IDNO:1, or (ii) the full-length complement of (i); (c) a polypeptideencoded by a polynucleotide having at least 60% identity to the maturepolypeptide coding sequence of SEQ ID NO:1; and (d) a fragment of themature polypeptide of SEQ ID NO:2, which has endoglucanase activity.

3. The detergent composition comprising an endoglucanase variant ofparagraph 2, wherein the parent endoglucanase having at least 60%, e.g.at least 65%, 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or 100%sequence identity to the mature polypeptide of SEQ ID NO:2.

4. The detergent composition comprising an endoglucanase variant of anyof paragraphs 2-3, wherein the parent endoglucanase is encoded by apolynucleotide that hybridizes under low stringency conditions, mediumstringency conditions, medium-high stringency conditions, highstringency conditions, or very high stringency conditions with (i) themature polypeptide coding sequence of SEQ ID NO:1 or (ii) thefull-length complement of (i).

5. The detergent composition comprising an endoglucanase variant of anyof paragraphs 2-4, wherein the parent endoglucanase is encoded by apolynucleotide having at least 60%, e.g. at least 65%, 70%, 75%, 80%,85%, 90%, 95%, 96%, 97%, 98%, 99%, or 100% sequence identity to themature polypeptide coding sequence of SEQ ID NO:1.

6. The detergent composition comprising an endoglucanase variant of anyof paragraphs 2-5, wherein the parent endoglucanase comprises orconsists of the mature polypeptide of SEQ ID NO:2.

7. The detergent composition comprising an endoglucanase variant of anyof paragraphs 2-6, wherein the parent endoglucanase is a fragment of themature polypeptide of SEQ ID NO:2, wherein the fragment hasendoglucanase activity.

8. The detergent composition comprising an endoglucanase variant of anyof paragraphs 2-7, which has at least 60%, e.g. at least 65%, 70%, 75%,80%, 85%, 90%, 95%, 96%, 97%, 98%, or 99%, but less than 100%, sequenceidentity to the amino acid sequence of the parent endoglucanase.

9. The detergent composition comprising an endoglucanase variant of anyof paragraphs 1-8, wherein said region selected from the groupconsisting of regions 1-9 is a chelator-induced instability region;

preferably said chelator-induced instability region (e.g. of SEQ ID NO:2or another parent endoglucanase) has one or more of the followingfeatures: (i) in the presence of a chelator it is less conformationallystable than one or more or all of its adjacent regions; and/or (ii) inthe presence of a chelator it is more exposed to said chelator than oneor more or all of its adjacent regions; and/or (iii) in the presence ofa chelator it is more accessible to said chelator than one or more orall of its adjacent regions; and/or (iv) in the presence of a chelatorit is more conformationally dynamic than one or more or all of itsadjacent regions; and/or (v) in the presence of a chelator it is morereceptive to deuterium incorporation than one or more or all of itsadjacent regions;

further preferably said adjacent region is selected from the groupconsisting of: (vi) region 10 corresponding to amino acids 1 to 94 ofSEQ ID NO:2; (vii) region 11 corresponding to amino acids 106 to 114 ofSEQ ID NO:2; (viii) region 12 corresponding to amino acids 139 to 209 ofSEQ ID NO:2; (ix) region 13 corresponding to amino acids 252 to 266 ofSEQ ID NO:2; (x) region 14 corresponding to amino acids 302 to 338 ofSEQ ID NO:2; (xi) region 15 corresponding to amino acids 362 to 546 ofSEQ ID NO:2; (xii) region 16 corresponding to amino acids 596 to 611 ofSEQ ID NO:2; (xiii) region 17 corresponding to amino acids 661 to 805 ofSEQ ID NO:2; (xiv) region 18 corresponding to amino acids 829 to 838 ofSEQ ID NO:2; and(xv) region 19 corresponding to amino acids 1043 to 1055of SEQ ID NO:2;

further most preferably said chelator is EDTA or citrate.

10. The detergent composition comprising an endoglucanase variant of anyone of paragraphs 1-9, wherein said variant further comprises analteration in at least one adjacent region, said adjacent region isselected from the group consisting of: (i′) region 10 corresponding toamino acids 1 to 94 of SEQ ID NO:2; (ii′) region 11 corresponding toamino acids 106 to 114 of SEQ ID NO:2; (iii′) region 12 corresponding toamino acids 139 to 209 of SEQ ID NO:2; (iv′) region 13 corresponding toamino acids 252 to 266 of SEQ ID NO:2; (v′) region 14 corresponding toamino acids 302 to 338 of SEQ ID NO:2; (vi′) region 15 corresponding toamino acids 362 to 546 of SEQ ID NO:2; (vii′) region 16 corresponding toamino acids 596 to 611 of SEQ ID NO:2; (viii′) region 17 correspondingto amino acids 661 to 805 of SEQ ID NO:2; (ix′) region 18 correspondingto amino acids 829 to 838 of SEQ ID NO:2; and (x′) region 19corresponding to amino acids 1043 to 1055 of SEQ ID NO:2.

11. The detergent composition comprising an endoglucanase variant of anyof paragraphs 1-10, wherein in an aqueous solution comprising adetergent component said region (e.g. of SEQ ID NO:2 or another parentendoglucanase) selected from the group consisting of regions 1-9 is lessconformationally stable than one or more or all of its adjacent regions;

preferably said adjacent region is selected from the group consistingof: (i) region 10 corresponding to amino acids 1 to 94 of SEQ ID NO:2;(ii) region 11 corresponding to amino acids 106 to 114 of SEQ ID NO:2;(iii) region 12 corresponding to amino acids 139 to 209 of SEQ ID NO:2;(iv) region 13 corresponding to amino acids 252 to 266 of SEQ ID NO:2;(v) region 14 corresponding to amino acids 302 to 338 of SEQ ID NO:2;(vi) region 15 corresponding to amino acids 362 to 546 of SEQ ID NO:2;(vii) region 16 corresponding to amino acids 596 to 611 of SEQ ID NO:2;(viii) region 17 corresponding to amino acids 661 to 805 of SEQ ID NO:2;(ix) region 18 corresponding to amino acids 829 to 838 of SEQ ID NO:2;and (x) region 19 corresponding to amino acids 1043 to 1055 of SEQ IDNO:2;

further preferably said detergent component comprises a chelator;further most preferably said chelator is EDTA or citrate.

12. The detergent composition comprising an endoglucanase variant of anyof paragraphs 1-11, wherein in an aqueous solution comprising adetergent component said region (e.g. of SEQ ID NO:2 or another parentendoglucanase) selected from the group consisting of regions 1-9 is moreexposed to said detergent component than one or more or all of itsadjacent regions;

preferably said adjacent region is selected from the group consistingof: (i) region 10 corresponding to amino acids 1 to 94 of SEQ ID NO:2;(ii) region 11 corresponding to amino acids 106 to 114 of SEQ ID NO:2;(iii) region 12 corresponding to amino acids 139 to 209 of SEQ ID NO:2;(iv) region 13 corresponding to amino acids 252 to 266 of SEQ ID NO:2;(v) region 14 corresponding to amino acids 302 to 338 of SEQ ID NO:2;(vi) region 15 corresponding to amino acids 362 to 546 of SEQ ID NO:2;(vii) region 16 corresponding to amino acids 596 to 611 of SEQ ID NO:2;(viii) region 17 corresponding to amino acids 661 to 805 of SEQ ID NO:2;(ix) region 18 corresponding to amino acids 829 to 838 of SEQ ID NO:2;and (x) region 19 corresponding to amino acids 1043 to 1055 of SEQ IDNO:2;

further preferably said detergent component comprises a chelator;further most preferably said chelator is EDTA or citrate.

13. The detergent composition comprising an endoglucanase variant of anyof paragraphs 1-12, wherein in an aqueous solution comprising adetergent component said region (e.g. of SEQ ID NO:2 or another parentendoglucanase) selected from the group consisting of regions 1-9 is moreaccessible to said detergent component than one or more or all of itsadjacent regions;

preferably said adjacent region is selected from the group consistingof: (i) region 10 corresponding to amino acids 1 to 94 of SEQ ID NO:2;(ii) region 11 corresponding to amino acids 106 to 114 of SEQ ID NO:2;(iii) region 12 corresponding to amino acids 139 to 209 of SEQ ID NO:2;(iv) region 13 corresponding to amino acids 252 to 266 of SEQ ID NO:2;(v) region 14 corresponding to amino acids 302 to 338 of SEQ ID NO:2;(vi) region 15 corresponding to amino acids 362 to 546 of SEQ ID NO:2;(vii) region 16 corresponding to amino acids 596 to 611 of SEQ ID NO:2;(viii) region 17 corresponding to amino acids 661 to 805 of SEQ ID NO:2;(ix) region 18 corresponding to amino acids 829 to 838 of SEQ ID NO:2;and (x) region 19 corresponding to amino acids 1043 to 1055 of SEQ IDNO:2;

further preferably said detergent component comprises a chelator;further most preferably said chelator is the EDTA or citrate.

14. The detergent composition comprising an endoglucanase variant of anyof paragraphs 1-13, wherein in an aqueous solution comprising adetergent component said region (e.g. of SEQ ID NO:2 or another parentendoglucanase) selected from the group consisting of regions 1-9 is moreconformationally dynamic than one or more or all of its adjacentregions;

preferably said adjacent region is selected from the group consistingof: (i) region 10 corresponding to amino acids 1 to 94 of SEQ ID NO:2;(ii) region 11 corresponding to amino acids 106 to 114 of SEQ ID NO:2;(iii) region 12 corresponding to amino acids 139 to 209 of SEQ ID NO:2;(iv) region 13 corresponding to amino acids 252 to 266 of SEQ ID NO:2;(v) region 14 corresponding to amino acids 302 to 338 of SEQ ID NO:2;(vi) region 15 corresponding to amino acids 362 to 546 of SEQ ID NO:2;(vii) region 16 corresponding to amino acids 596 to 611 of SEQ ID NO:2;(viii) region 17 corresponding to amino acids 661 to 805 of SEQ ID NO:2;(ix) region 18 corresponding to amino acids 829 to 838 of SEQ ID NO:2;and (x) region 19 corresponding to amino acids 1043 to 1055 of SEQ IDNO:2;

further preferably said detergent component comprises a chelator;further most preferably said chelator is EDTA or citrate.

15. The detergent composition comprising an endoglucanase variant of anyof paragraphs 1-14, wherein in an aqueous solution comprising adetergent component said region (e.g. of SEQ ID NO:2 or another parentendoglucanase) selected from the group consisting of regions 1-9 is morereceptive to deuterium incorporation than one or more or all of itsadjacent regions;

preferably said adjacent region is selected from the group consistingof: (i) region 10 corresponding to amino acids 1 to 94 of SEQ ID NO:2;(ii) region 11 corresponding to amino acids 106 to 114 of SEQ ID NO:2;(iii) region 12 corresponding to amino acids 139 to 209 of SEQ ID NO:2;(iv) region 13 corresponding to amino acids 252 to 266 of SEQ ID NO:2;(v) region 14 corresponding to amino acids 302 to 338 of SEQ ID NO:2;(vi) region 15 corresponding to amino acids 362 to 546 of SEQ ID NO:2;(vii) region 16 corresponding to amino acids 596 to 611 of SEQ ID NO:2;(viii) region 17 corresponding to amino acids 661 to 805 of SEQ ID NO:2;(ix) region 18 corresponding to amino acids 829 to 838 of SEQ ID NO:2;and (x) region 19 corresponding to amino acids 1043 to 1055 of SEQ IDNO:2;

further preferably said detergent component comprises a chelator;further most preferably said chelator is EDTA or citrate.

16. The detergent composition comprising an endoglucanase variant of anyof paragraphs 1-15, further comprising an alteration (e.g. asubstitution, deletion or insertion, preferably a substitution) at oneor more positions in:

a) one or more regions selected from the group consisting of:

i) region 1 corresponding to amino acids 95 to 105 of SEQ ID NO:2, e.g.said alteration at one or more positions selected from the groupconsisting of positions: 95, 96, 97, 98, 99, 100, 101, 102, 103, 104,105, wherein said positions correspond to amino acid positions of SEQ IDNO:2 (e.g. using the numbering of SEQ ID NO:2),

ii) region 2 corresponding to amino acids 115 to 138 of SEQ ID NO:2,e.g. said alteration at one or more positions selected from the groupconsisting of positions: 115, 116, 117, 118, 119, 120, 121, 122, 123,124, 125, 126, 127, 128, 129, 130, 131, 132, 133, 134, 135, 136, 137,138, wherein said positions correspond to amino acid positions of SEQ IDNO:2 (e.g. using the numbering of SEQ ID NO:2),

iii) region 3 corresponding to amino acids 210 to 251 of SEQ ID NO:2,e.g. said alteration at one or more positions selected from the groupconsisting of positions: 210, 211, 212, 213, 214, 215, 216, 217, 218,219, 220, 221, 222, 223, 224, 225, 226, 227, 228, 229, 230, 231, 232,233, 234, 235, 236, 237, 238, 239, 240, 241, 242, 243, 244, 245, 246,247, 248, 249, 250, 251, wherein said positions correspond to amino acidpositions of SEQ ID NO:2 (e.g. using the numbering of SEQ ID NO:2),

iv) region 4 corresponding to amino acids 267 to 301 of SEQ ID NO:2,e.g. said alteration at one or more positions selected from the groupconsisting of positions: 267, 268, 269, 270, 271, 272, 273, 274, 275,276, 277, 278, 279, 280, 281, 282, 283, 284, 285, 286, 287, 288, 289,290, 291, 292, 293, 294, 295, 296, 297, 298, 299, 300, 301, wherein saidpositions correspond to amino acid positions of SEQ ID NO:2 (e.g. usingthe numbering of SEQ ID NO:2),

v) region 5 corresponding to amino acids 339 to 361 of SEQ ID NO:2, e.g.said alteration at one or more positions selected from the groupconsisting of positions: 339, 340, 341, 342, 343, 344, 345, 346, 347,348, 349, 350, 351, 352, 353, 354, 355, 356, 357, 358, 359, 360, 361,wherein said positions correspond to amino acid positions of SEQ ID NO:2(e.g. using the numbering of SEQ ID NO:2),

vi) region 6 corresponding to amino acids 547 to 595 of SEQ ID NO:2,e.g. said alteration at one or more positions selected from the groupconsisting of positions: 547, 548, 549, 550, 551, 552, 553, 554, 555,556, 557, 558, 559, 560, 561, 562, 563, 564, 565, 566, 567, 568, 569,570, 571, 572, 573, 574, 575, 576, 577, 578, 579, 580, 581, 582, 583,584, 585, 586, 587, 588, 589, 590, 591, 592, 593, 594, 595, wherein saidpositions correspond to amino acid positions of SEQ ID NO:2 (e.g. usingthe numbering of SEQ ID NO:2),

vii) region 7 corresponding to amino acids 612 to 660 of SEQ ID NO:2,e.g. said alteration at one or more positions selected from the groupconsisting of positions: 612, 613, 614, 615, 616, 617, 618, 619, 620,621, 622, 623, 624, 625, 626, 627, 628, 629, 630, 631, 632, 633, 634,635, 636, 637, 638, 639, 640, 641, 642, 643, 644, 645, 646, 647, 648,649, 650, 651, 652, 653, 654, 655, 656, 657, 658, 659, 660, wherein saidpositions correspond to amino acid positions of SEQ ID NO:2 (e.g. usingthe numbering of SEQ ID NO:2),

viii) region 8 corresponding to amino acids 806 to 828 of SEQ ID NO:2,e.g. said alteration at one or more positions selected from the groupconsisting of positions: 806, 807, 808, 809, 810, 811, 812, 813, 814,815, 816, 817, 818, 819, 820, 821, 822, 823, 824, 825, 826, 827, 828,wherein said positions correspond to amino acid positions of SEQ ID NO:2(e.g. using the numbering of SEQ ID NO:2), and

ix) region 9 corresponding to amino acids 839 to 1042 of SEQ ID NO:2,e.g. said alteration at one or more positions selected from the groupconsisting of positions: 839, 840, 841, 842, 843, 844, 845, 846, 847,848, 849, 850, 851, 852, 853, 854, 855, 856, 857, 858, 859, 860, 861,862, 863, 864, 865, 866, 867, 868, 869, 870, 871, 872, 873, 874, 875,876, 877, 878, 879, 880, 881, 882, 883, 884, 885, 886, 887, 888, 889,890, 891, 892, 893, 894, 895, 896, 897, 898, 899, 900, 901, 902, 903,904, 905, 906, 907, 908, 909, 910, 911, 912, 913, 914, 915, 916, 917,918, 919, 920, 921, 922, 923, 924, 925, 926, 927, 928, 929, 930, 931,932, 933, 934, 935, 936, 937, 938, 939, 940, 941, 942, 943, 944, 945,946, 947, 948, 949, 950, 951, 952, 953, 954, 955, 956, 957, 958, 959,960, 961, 962, 963, 964, 965, 966, 967, 968, 969, 970, 971, 972, 973,974, 975, 976, 977, 978, 979, 980, 981, 982, 983, 984, 985, 986, 987,988, 989, 990, 991, 992, 993, 994, 995, 996, 997, 998, 999, 1000, 1001,1002, 1003, 1004, 1005, 1006, 1007, 1008, 1009, 1010, 1011, 1012, 1013,1014, 1015, 1016, 1017, 1018, 1019, 1020, 1021, 1022, 1023, 1024, 1025,1026, 1027, 1028, 1029, 1030, 1031, 1032, 1033, 1034, 1035, 1036, 1037,1038, 1039, 1040, 1041, 1042, wherein said positions correspond to aminoacid positions of SEQ ID NO:2 (e.g. using the numbering of SEQ ID NO:2);and/or

b) an adjacent region (e.g. an alteration at one or more positionscorresponding to positions: 51 (e.g, K51Q), 451 (e.g. K451S), 333 (e.g.W333L), 416 (e.g. Q416D)), preferably said adjacent region is selectedfrom the group consisting of: (i′) region 10 corresponding to aminoacids 1 to 94 of SEQ ID NO:2; (ii′) region 11 corresponding to aminoacids 106 to 114 of SEQ ID NO:2; (iii′) region 12 corresponding to aminoacids 139 to 209 of SEQ ID NO:2; (iv′) region 13 corresponding to aminoacids 252 to 266 of SEQ ID NO:2; (v′) region 14 corresponding to aminoacids 302 to 338 of SEQ ID NO:2; (vi′) region 15 corresponding to aminoacids 362 to 546 of SEQ ID NO:2; (vii′) region 16 corresponding to aminoacids 596 to 611 of SEQ ID NO:2; (viii′) region 17 corresponding toamino acids 661 to 805 of SEQ ID NO:2; (ix′) region 18 corresponding toamino acids 829 to 838 of SEQ ID NO:2; and (x′) region 19 correspondingto amino acids 1043 to 1055 of SEQ ID NO:2,

wherein said variant has at least 60%%, e.g. at least 65%, 70%, 75%,80%, 85%, 90%, 95%, 96%, 97%, 98%, or 99%, and less than 100% sequenceidentity to SEQ ID NO:2, preferably said variant has activity on xanthangum pretreated with xanthan lyase, further preferably said activity is axanthan gum degrading activity.

17. The detergent composition comprising an endoglucanase variant of anyof paragraphs 1-16, wherein said variant has at least 61%, 62%, 63%,64%, 65%, 66%, 67%, 68%, 69%, 70%, 71%, 72%, 73%, 74%, 75%, 76%, 77%,78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%,92%, 93%, 94%, 95%, 96%, 97%, 98%, or 99% sequence identity to SEQ IDNO:2.

18. The detergent composition comprising an endoglucanase variant of anyone of paragraphs 1-17, wherein said alteration at one or more positionis selected from the group consisting of alterations in positions: 4,17, 18, 20, 51, 53, 55, 56, 60, 63, 71, 79, 87, 92, 99, 120, 125, 126,130, 137, 182, 186, 189, 192, 213, 216, 221, 226, 228, 230, 231, 232,233, 235, 240, 243, 247, 249, 278, 279, 281, 283, 285, 289, 292, 294,298, 302, 311, 313, 333, 346, 353, 358, 386, 387, 388, 390, 403, 408,410, 416, 441, 448, 451, 471, 472, 476, 489, 507, 512, 515, 538, 555,556, 557, 558, 559, 560, 561, 562, 563, 564, 567, 568, 570, 575, 578,579, 580, 581, 583, 589, 590, 591, 592, 593, 595, 598, 599, 602, 603,605, 607, 609, 616, 627, 630, 631, 635, 636, 638, 639, 640, 641, 642,643, 644, 651, 676, 683, 688, 690, 694, 698, 699, 706, 711, 713, 1719,720, 744, 749, 754, 756, 760, , 781, 786, 797, 810, 811, 812, 815, 823,824, 825, 827, 828, 833, 834, 835, 837, 843, 848, 868, 869, 870, 871,872, 873, 874, 880, 881, 883, 884, 885, 887, 888, 890, 892, 894, 898,905, 906, 912, 920, 921, 924, 926, 927, 928, 932, 933, 934, 935, 937,938, 939, 940, 941, 942, 943, 946, 948, 950, 952, 953, 954, 956, 957,960, 966, 971, 972, 980, 989, 991, 994, 995, 998, 999, 1006, 1009, 1010,1011, 1029, 1030, 1031, 1032, 1035, 1037, 1038, 1040, 1041, 1042, 1044,1045, 1048, wherein numbering is according to SEQ ID NO:2.

19. The detergent composition comprising an endoglucanase variant of anyof paragraphs 1-18, wherein said alteration at one or more positions isselected from the group consisting of alterations in positions: 285,333, 353, *558, 558, 633, 635, 635, 635, 638, 639, 994, 281, 563, 575,575, 921, 558+559+560+561+562, 558, 559, 560, 561, 562 125, 126, 130,213, 221, 228, 228, 230, 230, 230, 230, 230, 230, 230, 231, 231, 232,232, 235, 240, 243, 243, 249, 278, 281, 281, 281, 281, 281, 281, 281,285, 285, 285, 285, 285, 285, 285, 285, 285, 285, 285, 292, 292, 292,292, 292, 292, 292, 292, 292, 292, 292, 292, 297, 346, 556, 558, 558,558, 558, 558, 558, 559, 559, 559, 559, 559, 559, 559, 559, 559, 559,559, 559, 560, 560, 560, 560, 561, 561, 561, 561, 561, 564, 564, 564,564, 564, 564, 565, 567, 568, 569, 569, 569, 569, 570, 570, 570, 570,570, 570, 570, 570, 570, 570, 570, 570, 570, 570, 570, 570, 570, 575,575, 576, 576, 576, 578, 579, 579, 580, 583, 589, 590, 590, 590, 591,592, 593, 593, 593, 593, 593, 593, 593, 593, 616, 627, 627, 627, 627,627, 627, 627, 630, 630, 630, 635, 635, 635, 635, 635, 635, 635, 636,636, 636, 636, 636, 636, 636, 636, 638, 638, 638, 638, 638, 639, 639,639, 639, 639, 639, 639, 639, 639, 641, 642, 642, 643, 643, 643, 644,651, 810, 811, 812, 812, 812, 812, 812, 815, 815, 815, 815, 815, 823,824, 825, 825, 825, 825, 827, 827, 827, 843, 870, 870, 870, 870, 870,870, 870, 870, 870, 870, 870, 870, 870, 870, 870, 870, 871, 871, 871,871, 871, 871, 871, 871, 871, 871, 871, 872, 872, 872, 872, 872, 872,872, 872, 872, 872, 872, 872, 872, 873, 873, 874, 874, 874, 874, 874,874, 874, 874, 881, 883, 884, 885, 885, 885, 887, 887, 887, 887, 887,894, 920, 921, 921, 932, 933, 933, 934, 934, 934, 934, 934, 934, 934,934, 934, 935, 937, 937, 937, 937, 937, 937, 937, 938, 939, 939, 940,941, 941, 941, 942, 942, 943, 943, 950, 950, 950, 952, 952, 953, 954,960, 964, 964, 966, 966, 971, 974, 974, 989, 991, 991, 991, 991, 991,991, 991, 995, 995, 995,995,995,998,998,1006,1006,1006,1006,1010,1011,1011,1011,1011,1011,1011,1011,1029,1030,1031,1031,1031,1031,1032,1035,1037,1037,1038,1038,1040,1040,1041,1044,1044,1044,1044,1045,1045, 559+579, 559,579, 564+579, 564,579, 559+579,559,579, 562+579, 562, 579, 564+579, 564,579, 559+579+99, 559,579,99,559+579+281, 559,579,281,281+559+579, 281, 559,579, 559+579+616,559,579,616, 559+579+636, 559,579, 636, 559+579+651, 559,579, 651,559+579+948, 559,579,948, 559+579+1009, 559,579,1009, 559+579+627,559,579,627, 579+921,579,921, 559+579+921,559,579,921,99+579,99,579,579+651,579,651,579+948,579,948,579+1009,579,1009, 559+579+934, 559,579,934, 559+579+921+934,559,579,921, 934, 559+579+627, 559,579,627, 559+579+627+616,559,579,627,616, 559+579+627, 559,579, 627, 559+579+921+651,559,579,921,651, 559+579+921+627, 559,579,921,627, 559+579+921+636,559,579,921, 636, 559+579+921+616, 559,579,921,616, 559+579+921+636,559,579,921, 636, 559+579+921+627+636, 559,579,921,627, 636,559+579+636+651, 559,579, 636,651, 559+579+616+651, 559,579,616,651,559+579+616+636, 559,579,616, 636, 559+579+616+921+934,559,579,616,921,934, 559+579+651+627, 559,579,651,627, 559+579+651+636,559,579,651, 636, 559+579+651+627+636, 559,579,651,627, 636,559+579+651+616, 559,579,651,616, 559+579+651+921+934,559,579,651,921,934, 636+934, 636,934, 636+921, 636,921, 636+627,636,627, 636+579, 636,579, 638+934, 638,934, 638+921, 638,921, 638+627,638,627, 638+579, 638,579,627+51,627, 51,627+451,627, 451,627+559, 627,559, 627+579, 627, 579, 579+934, 579, 934, 651+638, 651, 638, 570+651,570, 651, 570+921, 570, 921, 570+627, 570, 627, 570+559, 570, 559,570+579, 570, 579, 570+638, 570, 638, 570+579, 570, 579, 570+638, 570,638, 570+651, 570, 651, 570+636, 570, 636, 570+934, 570, 934, 570+638,570, 638, 570+921, 570, 921, 570+627, 570, 627, 570+559, 570, 559,570+885, 570, 885, 885+934, 885, 934, 885+627, 885, 627, 559+579+636,559, 579, 636, 559+579+638, 559, 579, 638, 559+579+870, 870,559+579+560, 560, 559+579+564, 564, 559+579+570, 570, 559+579+570, 570,559+579+570, 570, 559+579+570, 570, 559+579+570, 570, 559+579+570, 570,559+579+570, 570, 559+579+570, 570, 558, 559, 559, 559, 561, 564, 570,570, 570, 570, 570, 570, 570, 579, 579, 581, 616, 627, 627, 627, 636,636, 636, 636, 636, 636, 638, 638, 643, 651, 651, 885, 885, 921, 934,934, 966, 1011, 1031, 559+570+579, 559+570+579, 559+570+579,559+570+579, 559+570+579, 559+570+579, 559+570+579, 559+570+579,559+560+579, 559+579+651, 559+579+651+934, 559+579+638, 559+579+921,559+579+616+921, 559+579+636, 559+579, 559+579, 559+579+921,559+579+616, 638+934, 627+636, 627+934, 570+579, 416+559+579+636, 416,128+559+579+627, 128, 128+559+579+636, 579+636 of SEQ ID NO:2,preferably numbering is according to SEQ ID NO:2, further preferablyalterations in positions: 627, 636 or 638, wherein numbering isaccording to SEQ ID NO:2.

20. The detergent composition comprising an endoglucanase variant of anyof paragraphs 1-17, wherein said alteration at one or more positions isselected from the group consisting of: N285G, W333L, T353D, N558NP,N558F, T633V, D635L, D635M, D635T, F638Y, T639D, G994N, and K281T,G563E, I575M, I575A, K921D, N558K+A559K+S560F+T561P+G562W, N558K, A559K,S560F, T561P, G562W and I125V, A126R, K130R, K213R, A221R, K228E, K2281,G230F, G230L, G230A, G230H, G230N, G230W, G230T, F231Y, F231N, V232R,V232G, H235D, N240Q, G243K, G243R, A249N, A278S, K281F, K281V, K281Y,K281H, K281Q, K281N, K281W, N285L, N285M, N285S, N285P, N285T, N285Y,N285H, N285K, N285D, N285W, N285R, T292F, T292L, T292I, T292V, T292S,T292P, T292Y, T292Q, T292N, T292K, T292D, T292G, F297L, A346H, G556S,N558D, N558M, N558Q, N5581, N558Y, N558H, A559N, A559F, A559M, A559P,A559Y, A559H, A559Q, A559D, A559R, A559G, A5591, A559S, S560P, S560K,S560G, S560D, T561P, T561E, T561Q, T561S, T561D, A5641, A564Y, A564H,A564Q, A564K, A564E, E565M, V567F, K568R, L569F, L569Y, L569D, L569E,P570F, P570L, P570I, P570M, P570V, P570S, P570T, P570A, P570Y, P570H,P570Q, P570N, P570K, P570E, P570W, P570R, P570G, 1575D, I575E, I576F,I576M, I576P, D578R, Y579F, Y579W, V580L, D583M, Q589G, P590S, P590T,P590E, E591L, G592D, S593P, S593H, S593Q, S593N, S593K, S593D, S593E,S593R, S616D, K627L, K627M, K627V, K627S, K627T, K627Q, K627R, I630F,I630V, I630Y, D635A, D635P, D635N, D635K, D635E, D635G, D635W, S636L,S636M, S636A, S636H, S636Q, S636N, S636K, S636R, F6381, F638V, F638T,F638L, F638H, T639V, T639S, T639L, T6391, T639M, T639A, T639E, T639W,T639G, Y641E, S642T, S642N, N643D, N643H, N643T, T644F, A651P, S810R,A811S, V812F, V812I, V812M, V812W, V812R, N815V, N815Y, N815E, N815W,N815R, S823Q, A824T, T825N, T825W, T825A, T825D, V827I, V827M, V827S,T843V, D870F, D870L, D870I, D870M, D870V, D870S, D870T, D870Y, D870H,D870Q, D870N, D870K, D870E, D870W, D870R, D870G, P871F, P871L, P871I,P871M, P871V, P871S, P871T, P871A, P871Y, P871H, P871Q, T872S, T872F,T872A, T872Y, T872H, T872Q, T872N, T872K, T872D, T872E, T872W, T872R,T872G, D873K, D873E, T874V, T874S, T874P, T874A, T874H, T874Q, T874N,T874K, V881Q, T883K, Y884H, A885F, A885Q, A885N, T887L, T887I, T887S,T887H, T887R, K894E, N920D, K921R, K921E, T932A, N933V, N933S, Y934G,Y934M, Y934S, Y934A, Y934Q, Y934N, Y934E, Y934W, Y934R, T935W, A937F,A937V, A937S, A937T, A937Q, A937D, A937E, V9381, K939I, K939V, D940E,N941S, N941H, N941D, A942P, A942E, D943Y, D943H, R950V, R950H, R950N,F952S, F952W, N953Y, G954L, Y960F, A964N, A964C, N966P, N966C, G971A,Q974K, Q974C, Q9891, Q991L, Q991I, Q991M, Q991V, Q991T, Q991K, Q991C,S995I, S995V, S995Q, S995R, S995C, G998V, G998A, S1006T, S1006A, S1006K,S1006R, Y1010W, L1011M, L1011S, L1011A, L1011Q, L1011N, L1011D, L1011E,R1029N, F1030M, K10311, K1031S, K1031T, K1031H, V1032G, K1035A, A1037E,A1037W, S1038L, S1038I, L1040N, L1040E, G1041F, L1044F, L1044S, L1044N,L1044W, P1045Q, P1045W, and A559N+Y579F, A559N, Y579F, A564E+Y579F,A564E, Y579F, A559N+Y579W, A559N, Y579W, G562P+Y579W, G562P, Y579W,A564D+Y579W, A564D, Y579W, A559N+Y579W+K99R, A559N, Y579W, K99R,A559N+Y579W+K281R, A559N, Y579W, K281R, K281R+A559N+Y579W, K281R, A559N,Y579W, A559N+Y579W+S616D, A559N, Y579W, S616D, A559N+Y579W+S636N, A559N,Y579W, S636N, A559N+Y579W+A651P, A559N, Y579W, A651P, A559N+Y579W+K948E,A559N, Y579W, K948E, A559N+Y579W+K1009E, A559N, Y579W, K1009E,A559N+Y579W+K627R, A559N, Y579W, K627R, Y579W+K921R, Y579W, K921R,A559N+Y579W+K921R, A559N, Y579W, K921R, K99R+Y579W, K99R, Y579W,Y579W+A651P, Y579W, A651P, Y579W+K948E, Y579W, K948E, Y579W+K1009E,Y579W, K1009E, A559N+Y579W+Y934G, A559N, Y579W, Y934G,A559N+Y579W+K921R+Y934G, A559N, Y579W, K921R, Y934G, A559N+Y579W+K627M,A559N, Y579W, K627M, A559N+Y579W+K627R+S616D, A559N, Y579W, K627R,S616D, A559N+Y579F+K627R, A559N, Y579F, K627R, A559N+Y579W+K921R+A651P,A559N, Y579W, K921R, A651P, A559N+Y579W+K921R+K627R, A559N, Y579W,K921R, K627R, A559N+Y579W+K921R+S636K, A559N, Y579W, K921R, S636K,A559N+Y579W+K921R+S616D, A559N, Y579W, K921R, S616D,A559N+Y579W+K921R+S636N, A559N, Y579W, K921R, S636N,A559N+Y579W+K921R+K627R+S636N, A559N, Y579W, K921R, K627R, S636N,A559N+Y579W+S636N+A651P, A559N, Y579W, S636N, A651P,A559N+Y579W+S616D+A651P, A559N, Y579W, S616D, A651P,A559N+Y579W+S616D+S636K, A559N, Y579W, S616D, S636K,A559N+Y579W+S616D+K921R+Y934G, A559N, Y579W, S616D, K921R, Y934G,A559N+Y579W+A651P+K627M, A559N, Y579W, A651P, K627M,A559N+Y579W+A651P+S636K, A559N, Y579W, A651P, S636K,A559N+Y579W+A651P+K627R+S636N, A559N, Y579W, A651P, K627R, S636N,A559N+Y579W+A651P+S616D, A559N, Y579W, A651P, S616D,A559N+Y579W+A651P+K921R+Y934G, A559N, Y579W, A651P, K921R, Y934G,S636N+Y934G, S636N, Y934G, S636N+K921R, S636N, K921R, S636N+K627R,S636N, K627R, S636N+Y579W, S636N, Y579W, F6381+Y934G, F638I, Y934G,F638I+K921R, F638I, K921R, F6381+K627R, F638I, K627R, F6381+Y579W,F6381, Y579W, K627R+K51Q, K627R, K51Q, K627R+K451S, K627R, K451S,K627R+A559N, K627R, A559N, K627R+Y579W, K627R, Y579W, Y579W+Y934G,Y579W, Y934G, A651P+F638I, A651P, F638I, P570Q+A651P, P570Q, A651P,P570Q+K921R, P570Q, K921R, P570Q+K627R, P570Q, K627R, P570Q+A559N,P570Q, A559N, P570Q+Y579W, P570Q, Y579W, P570Q+F638I, P570Q, F638I,P570K+Y579W, P570K, Y579W, P570K+F6381, P570K, F638I, P570T+A651P,P570T, A651P, P570T+S636N, P570T, S636N, P570T+Y934G, P570T, Y934G,P570T+F638I, P570T, F638I, P570T+K921R, P570T, K921R, P570T+K627R,P570T, K627R, P570T+A559N, P570T, A559N, P570T+A885F, P570T, A885F,A885F+Y934G, A885F, Y934G, A885F+K627R, A885F, K627R, A559N+Y579W+S636L,A559N, Y579W, S636L, A559N+Y579W+F638I, A559N, Y579W, F638I,A559N+Y579W+D870M, D870M, A559N+Y579W+S560P, 5560P, A559N+Y579W+A564I,A564I, A559N+Y579W+P570N, P570N,

A559N+Y579W+P570K, P570K, A559N+Y579W+P570R, P570R, A559N+Y579W+P570A,P570A, A559N+Y579W+P570T, P570T, A559N+Y579W+P570S, P570S,A559N+Y579W+P570Q, P570Q, A559N+Y579W+P570H, P570H, and N558E, A559P,A559N, A559H, T561P, A564E, P570A, P570Q, P570R, P570S, P570K, P570T,P570N, Y579W, Y579F, T581M, S616D, K627R, K627M, K627Q, S636N, S636Q,S636R, S636K, S636M, S636H, F638I, F638L, N643D, A651P, A651S, A885F,A885Q, K921R, Y934R, Y934G, N966C, L1011A, K1031I, andA559N+P570A+Y579W, A559N+P570H+Y579W, A559N+P570K+Y579W,A559N+P570N+Y579W, A559N+P570Q+Y579W, A559N+P570R+Y579W,A559N+P570K+Y579W, A559N+P570T+Y579W, A559N+S560P+Y579W,A559N+Y579W+A651P, A559N+Y579W+A651P+Y934G, A559N+Y579W+F638I,A559N+Y579W+K921R, A559N+Y579W+S616D+K921R, A559N+Y579W+S636N,A559N+Y579F, A559N+Y579W, A559N+Y579W+K921R, A559N+Y579W+S616D,F638I+Y934G, K627R+S636N, K627R+Y934G, P570K+Y579W,Q416D+A559N+Y579W+S636N, Q416D, S128X+A559N+Y579W+K627R, S128X,S128X+A559N+Y579W+S636N, Y579W+S636N, V4T, S17A, N18G, F20P, F20N, F20G,F20Y, K51Q, K51H, E53Y, E53P, E53G, Y55M, Y55D, V56M, Y60F, S63F, A71E,579W, T87R, T92S, A120P, N129D, F137L, H182Y, A186P, N189K, K192N,N216D, N216Q, N216R, L226K, G230H, L233H, D247N, G279E, K281R, A283D,N285D, N285G, Q289E, T292A, T292F, T292Y, A294V, Q298E, I302D, I302H,I302V, I302M, H311N, S313D, A346D, A386P, I387T, K388R, K390Q, 1403Y,E408D, E408N, E4085, E408P, E408A, E408G, P410G, Q4165, Q416D, N441G,A448E, A448W, A4485, K451S, K451Q, G471S, S472Y, D476R, Q489P, K507R,K512P, S515V, S538C, L555Q, G557R, N558E, A559N, A559P, A559H, A559D,S560P, S560G, T561P, A564E, A5641, V567P, K568R, P570R, P570Q, P570K,P570A, P570T, P570G, P570S, P570H, P570N, I575V, Y579W, Y579F, T581M,S593N, S593E, S595L, S598Q, A599S, I602T, I602D, V603P, S605T, S607C,G609E, S616G, S616D, K627R, K627M, K627Q, K631R, K631A, D635A, D635E,D635M, D635N, D635L, D635W, S636N, S636K, S636L, S636Q, S636R, S636M,5636H, F638N, F638I, F638L, F638V, F638H, F638M, T639G, T639I, T639M,T639Y, T639W, T639P, T639E, T640S, S642N, S642T, N643D, N643H, A651P,A651S, D676H, Q683E, A688G, Y690F, T694A, T697G, R698W, T699A, T706Q,T711S, T711V, T711Y, K713R, W719R, K720H, K744H, K744Q, A749T, K754R,V756Y, V756H, S760G, T, 781M, N786K, T797S, S810Q, A824D, T825G, N828D,N833D, Q834E, S835A, S835D, V837I, N848D, A868E, A869V, D870V, T872G,T872H, T872W, T872Q, R880K, V881Q, V881T, T883R, T883V, T883C, T883K,Y884H, A885N, A885Q, A885F, T887K, T887S, L888M, V890R, T892P, T892V,R898Q, N905D, F906A, Q912V, N920P, K921R, A924D, V926F, V926P, K927R,S928D, T932A, N933S, N933V, Y934G, Y934R, Y934Q, A937E, V938I, K939V,N941S, A942P, G946R, K948R, Q956Y, Q956S, A957L, A957P, N966C, T972K,M9801, G994D, T999R, L1011A, K1031I, A1037E, S1038G, G1041R, Y1042N,F1048W, preferably numbering is according to SEQ ID NO:2, furtherpreferably alterations in positions: K627R, S636N or F6381, whereinnumbering is according to SEQ ID NO:2.

21. The detergent composition comprising an endoglucanase variant of anyof paragraphs 1-20, wherein said alteration at one or more positions isselected from the group consisting of alterations in positions: 17, 20,51, 53, 55, 56, 60, 63, 79, 87, 192, 302, 387, 388, 390, 403, 408, 410,416, 448, 451, 471, 472, 507, 512, 515, 538, 598, 602, 605, 609, 676,694, 698, 699, 711, 754, 760, , 781, 786, 797, 834, and 835 of SEQ IDNO:2, wherein numbering is according to SEQ ID NO:2.

22. The detergent composition comprising an endoglucanase variant of anyof paragraphs 1-21, wherein said alteration at one or more positions isselected from the group consisting of: 517A, F20P, F20N, F20G, F20Y,K51Q, K51H, E53P, E53G, Y55M, V56M, Y60F, S63F, T87R, K192N, I302H,I302V, I302M, I387T, K388R, K390Q, I403Y, E408D, E408S, E408P, E408A,E408G, E408N, P410G, Q416S, Q416D, A448E, A448W, A4485, K451S, G471S,S472Y, K507R, K512P, S515V, S538C, Y579W, S598Q, I602T, I602D, S605T,G609E, D676H, T694A, R698W, T699A, T711V, T711Y, K754R, 5760G, T, 781M,N786K, T797S, Q834E, and S835D of SEQ ID NO:2.

23. The detergent composition comprising an endoglucanase variant of anyof paragraphs 1-22, wherein the total number of alterations compared tothe parent endoglucanase (e.g. SEQ ID NO:2) is between 1 and 20, e.g.between 1 and 18 or between 5 and 16 or from 8 to 14, such as 1, 2, 3,4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, or 16 alterations.

24. The detergent composition comprising an endoglucanase variant of anyof paragraphs 1-23, wherein said activity on xanthan gum pretreated withxanthan lyase is a xanthan degrading activity, preferably said xanthandegrading activity is endoglucanase EC 3.2.1.4 activity.

25. The detergent composition comprising an endoglucanase variant of anyof paragraphs 1-24, wherein said variant has an improved stability in adetergent composition compared to a parent endoglucanase (e.g. with SEQID NO:2); optionally said detergent composition comprises a chelator;further preferably said chelator is EDTA or citrate.

26. The detergent composition comprising an endoglucanase variant of anyof paragraphs 1-25, wherein said variant has a half-life improvementfactor (HIF) of 1.0; preferably said variant has a half-life improvementfactor (HIF) of >1.0 relative to a parent endoglucanase, e.g. anendoglucanase of SEQ ID NO:2.

27. The detergent composition comprising an endoglucanase variant ofparagraph 24, wherein said half-life improvement factor (HIF) isdetermined after incubation of said endoglucanase variant in a detergentcomposition at 25° C. for a time period from about 5-140 h or from about17-20 h.

28. The detergent composition of any one of paragraphs 1-27, wherein thexanthan lyase variant is a variant of a parent xanthan lyase selectedfrom the group consisting of: a) a polypeptide having at least 60%sequence identity to the mature polypeptide of SEQ ID NO:6; b) apolypeptide encoded by a polynucleotide that hybridizes under lowstringency conditions with (i) the mature polypeptide coding sequence ofSEQ ID NO:5, or (ii) the full-length complement of (i); c) a polypeptideencoded by a polynucleotide having at least 60% identity to the maturepolypeptide coding sequence of SEQ ID NO:5; and d) a fragment of themature polypeptide of SEQ ID NO:6, which has xanthan lyase activity.

29. The detergent composition of paragraph 28, wherein the parentxanthan lyase has at least 60%, e.g. at least 65%, 70%, 75%, 80%, 85%,90%, 95%, 96%, 97%, 98%, 99% or 100% sequence identity to the maturepolypeptide of SEQ ID NO:6.

30. The detergent composition of any of paragraphs 28-29, wherein theparent xanthan lyase is encoded by a polynucleotide that hybridizesunder low stringency conditions, medium stringency conditions,medium-high stringency conditions, high stringency conditions, or veryhigh stringency conditions with (i) the mature polypeptide codingsequence of SEQ ID NO:5 or (ii) the full-length complement of (i).

31. The detergent composition of any of paragraphs 28-30, wherein theparent xanthan lyase is encoded by a polynucleotide having at least 60%,e.g. at least 65%, 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99%, or100% sequence identity to the mature polypeptide coding sequence of SEQID NO:5.

32. The detergent composition of any of paragraphs 28-31, wherein theparent xanthan lyase comprises or consists of the mature polypeptide ofSEQ ID NO:6.

33. The detergent composition of any of paragraphs 28-32, wherein theparent xanthan lyase is a fragment of the mature polypeptide of SEQ IDNO:6, wherein the fragment has xanthan lyase activity.

34. The detergent compositionof any of paragraphs 28-33, wherein thexanthan lyase variant has at least 60%, e.g. at least 65%, 70%, 75%,80%, 85%, 90%, 95%, 96%, 97%, 98%, or 99%, but less than 100%, sequenceidentity to the amino acid sequence of the parent xanthan lyase.

35. The detergent composition of any of paragraphs 1-34, wherein saidregion of the xanthan lyase selected from the group consisting ofregions 1-6 is a chelator-induced instability region;

preferably said chelator-induced instability region (e.g. of SEQ ID NO:6or another parent xanthan lyase) has one or more of the followingfeatures: (i) in the presence of a chelator it is less conformationallystable than one or more or all of its adjacent regions; and/or (ii) inthe presence of a chelator it is more exposed to said chelator than oneor more or all of its adjacent regions; and/or (iii) in the presence ofa chelator it is more accessible to said chelator than one or more orall of its adjacent regions; and/or (iv) in the presence of a chelatorit is more conformationally dynamic than one or more or all of itsadjacent regions; and/or (v) in the presence of a chelator it is morereceptive to deuterium incorporation than one or more or all of itsadjacent regions;

further preferably said adjacent region is selected from the groupconsisting of: (vi) region 7 corresponding to amino acids 1 to 153 ofSEQ ID NO:6; (vii) region 8 corresponding to amino acids 177 to 613 ofSEQ ID NO:6; (viii) region 9 corresponding to amino acids 659 to 730 ofSEQ ID NO:6; (ix) region 10 corresponding to amino acids 804 to 806 ofSEQ ID NO:6; (x) region 11 corresponding to amino acids 847 to 871 ofSEQ ID NO:6; (xi) region 12 corresponding to amino acids 886 to 902 ofSEQ ID NO:6; and (xii) region 13 corresponding to amino acids 1005 to1037 of SEQ ID NO:6;

said chelator optionally being EDTA or citrate.

36. The detergent composition of any of paragraphs 1-35, wherein in anaqueous solution comprising a detergent component said region of thexanthan lyase (e.g. of SEQ ID NO:6 or another parent xanthan lyase)selected from the group consisting of regions 1-6 is lessconformationally stable than one or more or all of its adjacent regions;

preferably said adjacent region is selected from the group consistingof: (i) region 7 corresponding to amino acids 1 to 153 of SEQ ID NO:6;(ii) region 8 corresponding to amino acids 177 to 613 of SEQ ID NO:6;(iii) region 9 corresponding to amino acids 659 to 730 of SEQ ID NO:6;(iv) region 10 corresponding to amino acids 804 to 806 of SEQ ID NO:6;(v) region 11 corresponding to amino acids 847 to 871 of SEQ ID NO:6;(vi) region 12 corresponding to amino acids 886 to 902 of SEQ ID NO:6;and (vii) region 13 corresponding to amino acids 1005 to 1037 of SEQ IDNO:6,

said detergent component optionally comprising a chelator; optionallysaid chelator being EDTA or citrate.

37. The detergent composition of any of paragraphs 1-36, wherein in anaqueous solution comprising a detergent component said region of thexanthan lyase (e.g. of SEQ ID NO:6 or another parent xanthan lyase)selected from the group consisting of regions 1-6 is more exposed tosaid detergent component than one or more or all of its adjacentregions;

preferably said adjacent region is selected from the group consistingof: (i) region 7 corresponding to amino acids 1 to 153 of SEQ ID NO:6;(ii) region 8 corresponding to amino acids 177 to 613 of SEQ ID NO:6;(iii) region 9 corresponding to amino acids 659 to 730 of SEQ ID NO:6;(iv) region 10 corresponding to amino acids 804 to 806 of SEQ ID NO:6;(v) region 11 corresponding to amino acids 847 to 871 of SEQ ID NO:6;(vi) region 12 corresponding to amino acids 886 to 902 of SEQ ID NO:6;and (vii) region 13 corresponding to amino acids 1005 to 1037 of SEQ IDNO:6,

said detergent component optionally comprising a chelator; said chelatoroptionally being EDTA or citrate.

38. The detergent composition of any of paragraphs 1-37, wherein in anaqueous solution comprising a detergent component said region of thexanthan lyase (e.g. of SEQ ID NO:6 or another parent xanthan lyase)selected from the group consisting of regions 1-6 is more accessible tosaid detergent component than one or more or all of its adjacentregions;

preferably said adjacent region is selected from the group consistingof: (i) region 7 corresponding to amino acids 1 to 153 of SEQ ID NO:6,(ii) region 8 corresponding to amino acids 177 to 613 of SEQ ID NO:6,(iii) region 9 corresponding to amino acids 659 to 730 of SEQ ID NO:6,(iv) region 10 corresponding to amino acids 804 to 806 of SEQ ID NO:6,(v) region 11 corresponding to amino acids 847 to 871 of SEQ ID NO:6,(vi) region 12 corresponding to amino acids 886 to 902 of SEQ ID NO:6,and (vii) region 13 corresponding to amino acids 1005 to 1037 of SEQ IDNO:6,

said detergent component optionally comprising a chelator; said chelatoroptionally being EDTA or citrate.

39. The detergent composition of any of paragraphs 1-38, wherein in anaqueous solution comprising a detergent component said region of thexanthan lyase (e.g. of SEQ ID NO:6 or another parent xanthan lyase)selected from the group consisting of regions 1-6 is moreconformationally dynamic than one or more or all of its adjacentregions;

preferably said adjacent region is selected from the group consistingof: (i) region 7 corresponding to amino acids 1 to 153 of SEQ ID NO:6,(ii) region 8 corresponding to amino acids 177 to 613 of SEQ ID NO:6,(iii) region 9 corresponding to amino acids 659 to 730 of SEQ ID NO:6,(iv) region 10 corresponding to amino acids 804 to 806 of SEQ ID NO:6,(v) region 11 corresponding to amino acids 847 to 871 of SEQ ID NO:6,(vi) region 12 corresponding to amino acids 886 to 902 of SEQ ID NO:6,and (vii) region 13 corresponding to amino acids 1005 to 1037 of SEQ IDNO:6,

said detergent component optionally comprising a chelator; said chelatoroptionally being EDTA or citrate.

40. The detergent composition of any of paragraphs 1-39, wherein in anaqueous solution comprising a detergent component said region of thexanthan lyase (e.g. of SEQ ID NO:6 or another parent xanthan lyase)selected from the group consisting of regions 1-6 is more receptive todeuterium incorporation than one or more or all of its adjacent regions;

preferably said adjacent region is selected from the group consistingof: (i) region 7 corresponding to amino acids 1 to 153 of SEQ ID NO:6,(ii) region 8 corresponding to amino acids 177 to 613 of SEQ ID NO:6,(iii) region 9 corresponding to amino acids 659 to 730 of SEQ ID NO:6,(iv) region 10 corresponding to amino acids 804 to 806 of SEQ ID NO:6,(v) region 11 corresponding to amino acids 847 to 871 of SEQ ID NO:6,(vi) region 12 corresponding to amino acids 886 to 902 of SEQ ID NO:6,and (vii) region 13 corresponding to amino acids 1005 to 1037 of SEQ IDNO:6,

said detergent component optionally comprising a chelator; said chelatoroptionally being EDTA or citrate.

41. The detergent compositionof any of paragraphs 1-40, the xanthanlyase variant comprising an alteration (e.g. a substitution, deletion orinsertion, preferably a substitution) at one or more positions in two ormore regions selected from the group consisting of: (i) region 1corresponding to amino acids 154 to 176 of SEQ ID NO:6, (ii) region 2corresponding to amino acids 614 to 658 of SEQ ID NO:6, (iii) region 3corresponding to amino acids 731 to 803 of SEQ ID NO:6, (iv) region 4corresponding to amino acids 807 to 846 of SEQ ID NO:6, (v) region 5corresponding to amino acids 872 to 885 of SEQ ID NO:6, (vi) region 6corresponding to amino acids 903 to 1004 of SEQ ID NO:6,

wherein said variant has at least 60%, e.g. at least 65%, 70%, 75%, 80%,85%, 90%, 95%, 96%, 97%, 98%, or 99%, and less than 100% sequenceidentity to SEQ ID NO:6, preferably said variant has activity on xanthangum, further preferably said activity is a xanthan gum degradingactivity.

42. The detergent composition of any of paragraphs 1-41, wherein saidxanthan lyase variant has at least 61%, 62%, 63%, 64%, 65%, 66%, 67%,68%, 69%, 70%, 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%,82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%,96%, 97%, 98%, or 99% sequence identity to SEQ ID NO:6.

43. The detergent composition of any of paragraphs 1-42, wherein saidalteration of the xanthan lyase (e.g. a substitution, deletion orinsertion, preferably a substitution) at one or more positions isselected from the group consisting of alterations in positions: 155,159, 620, 624, 626, 631, 635, 645, 649, 650, 656, 738, 745, 746, 748,752, 753, 754, 757, 764, 769, 774, 775, 777, 779, 782, 785, 786, 789,792, 796, 799, 800, 801, 819, 824, 843, 845, 875, 903, 911, 912, 915,919, 921, 923, 925, 927, 928, 930, 932, 933, 941, 966, 967, 991 and 998.of SEQ ID NO:6, wherein numbering is according to SEQ ID NO:6,preferably alterations in positions: 775, 779 or 923, wherein numberingis according to SEQ ID NO:6.

44. The detergent composition of any of paragraphs 1-43, wherein saidalteration of the xanthan lyase at one or more positions is selectedfrom the group consisting of: Y155E, A159P, K620R, A624E, A626G, T631N,T631E, S635E, S635T, S635Q, A645S, T649V, T649K, T649R, Q650G, I656V,G738L, K745R, F746L, L748T, P752R, P752K, G753E, G753Q, G753S, S754E,S754L, S754Q, S754R, S757D, S757P, S757E, P764V, P764K, A769D, A769T,A769R, A769S, A769E, A769Q, A769*, A774V, L775M, L775Y, L775A, L7751,L775S, L775F, L775Q, D777K, D777R, P779V, Y782I, A785T, N786K, G789R,K792W, K792Y, K792V, K792A, N796Q, A799H, V800P, D801G, K819R, K819T,K824R, A843P, D845E, K875T, K875E, T903A, T903Q, A911V, A911M, A911S,A912T, A912I, A912Y, T915Q, T915S, T915V, T915A, T919F, T919G, T919D,T921R, T921S, T923H, T923D, T925Q, T925D, T925R, T927K, D928W, Y930H,Y930L, Y930F, A932P, D933M, G941E, G941D, A966P, A967D, N991D and V998K,wherein numbering is according to SEQ ID NO:6.

45. The detergent composition of any of paragraphs 1-44, comprising analteration of the xanthan lyase at one or more positions in at least oneregion selected from the group consisting of: (vii) region 7corresponding to amino acids 1 to 153 of SEQ ID NO:6, (viii) region 8corresponding to amino acids 177 to 613 of SEQ ID NO:6, (ix) region 9corresponding to amino acids 659 to 730 of SEQ ID NO:6, (x) region 10corresponding to amino acids 804 to 806 of SEQ ID NO:6, (xi) region 11corresponding to amino acids 847 to 871 of SEQ ID NO:6, (xii) region 12corresponding to amino acids 886 to 902 of SEQ ID NO:6, and (xiii)region 13 corresponding to amino acids 1005 to 1037 of SEQ ID NO:6.

46. The detergent composition of paragraph 45, wherein said alterationof the xanthan lyase at one or more positions in at least one regionselected from the group consisting of regions 7, 8, 9, 10, 11, 12 and 13is an alteration at one or more positions selected from the groupconsisting of: 9, 15, 18, 46, 58, 66, 89, 95, 100, 106, 109, 183, 188,190, 203, 204, 221, 229, 234, 238, 240, 242, 243, 257, 258, 284, 291,293, 316, 317, 320, 324, 329, 333, 339, 341, 352, 354, 360, 372, 377,399, 400, 419, 440, 450, 451, 454, 458, 481, 492, 505, 533, 567, 568,576, 578, 579, 582, 664, 672, 703, 722, 726, 727, 728, 851, 855, 856,867, 887, 892, 899, 900, 901, 902, 915, 1008 and 1016 of SEQ ID NO:6.

47. The detergent composition of paragraph 46, wherein said alterationof the xanthan lyase at one or more positions in at least one regionselected from the group consisting of regions 7, 8, 9, 10, 11, 12 and 13comprises one or more substitutions selected from the group consistingof: K9R, N15T, T18D, L46D, A58L, S66H, Q89Y, K95E, S100D, N106Y, Q109R,Q109D, Q109F, Q109K, Q109A, K183Q, K183R, V188I, A190Q, A203P, K204R,A221P, E229N, E229S, E229V, I234V, I238W, I238L, I238M, I240W, N242S,G243V, Y257W, R258E, R284G, K291R, A293G, A293P, K316R, R317K, K320R,L324Q, K329R, K333R, L339M, I341P, V352I, S354P, K360G, K360R, Q372H,F377Y, N399K, K400R, F419Y, N440K, D450P, K451E, K451R, A454V, D458S,K481R, A492H, A492L, T505I, L533I, K567R, G568A, S578K, S578N, S578R,S579R, S579K, S582K, T664K, N672D, I703L, I722F, P726Q, T727P, M728V,S851F, K855R, E856D, P867S, K887R, N892Y, N892W, N892F, G899S, I1900G,D901A, T902F, N1008D and K1016T of SEQ ID NO:6.

48. The detergent composition of any of paragraphs 1-47, the xanthanlyase variant comprising an alteration at one or more positions selectedfrom the group consisting of positions 624, 631, 635, 649, 656, 738,752, 753, 754, 757, 769, 775, 777, 800, 801, 843, 875, 911 and 915, andan alteration at one or more positions selected from the groupconsisting of positions 89, 100, 190, 229, 234, 352, 360, 399, 440, 458,492, 567, 582, 664, 672, 703, 728, 892, 1008 and 1016 of SEQ ID NO:6.

49. The detergent composition of paragraph 48, the xanthan lyase variantcomprising one or more substitutions selected from the group consistingof Q89Y, S100D, A190Q, E229S, 1234V, V352I, K360G, N399K, N440K, D458S,A492H, A492L, K567R, S582K, T664K, N672D, I703L, M728V, N892Y N1008D andK1016T, and one or more substitutions selected from the group consistingof A624E, T631N, S635E, T649K, I656V, G738L, P752K, P752R, G753E, S754E,S754R, S757D, A769D, L775A, D777R, V800P, D801G, A843P, K875T, A911V andT915A.

50. The detergent composition of any of paragraphs 1-49, wherein saidalteration of the xanthan lyase (e.g. a substitution, deletion orinsertion, preferably a substitution) at one or more positions isselected from the group consisting of alterations in positions: 9, 15,46, 58, 66, 89, 95, 100, 106, 109, 183, 188, 190, 203, 204, 221, 229,234, 238, 240, 242, 243, 257, 258, 291, 293, 316, 320, 324, 329, 333,339, 341, 352, 354, 360, 377, 400, 419, 450, 451, 454, 481, 492, 567,568, 578, 579, 664, 672, 855, 887 and 892 of SEQ ID NO:6, whereinnumbering is according to SEQ ID NO:6.

51. The detergent composition of any of paragraphs 1-50, wherein thexanthan lyase variant has one or more substitutions selected from thegroup consisting of: K9R, N15T, L46D, A58L, S66H, Q89Y, K95E, S100D,N106Y, Q109R, Q109D, Q109F, Q109K, Q109A, K183Q,K183R, V188I, A190Q,A203P, K204R, A221P, E229N, E229S, I234V, I238W, I238L, I238M, I240W,N242S, G243V, Y257W, R258E, K291R, A293G, A293P, K316R, K320R, L324Q,K329R, K333R, L339M, 1341P, V352I, S354P, K360R, F377Y, K400R, F419Y,D450P, K451E, K451R, A454V, K481R, A492L, K567R, G568A, S578K, S578R,S579R, S579K, T664K, N672D, K855R, K887R, N892Y, N892W and N892F,wherein numbering is according to SEQ ID NO:6.

52. The detergent composition of any of paragraphs 1-51, wherein saidxanthan lyase variant does not comprise any amino acid alteration at aposition outside of regions 7, 8, 9, 10, 11, 12 and 13.

53. The detergent composition of any of paragraphs 1-52, wherein thetotal number of alterations of the xanthan lyase compared to the parentxanthan lyase (e.g. SEQ ID NO:6) is from 1 to 20, e.g. from 1 to 18 orfrom 5 to 17 or from 8 to 16, such as 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11,12, 13, 14, 15, 16, 17 or 18 alterations.

54. The detergent composition of any of paragraphs 1-53, wherein saidactivity on xanthan gum is a xanthan gum degrading activity, preferablysaid xanthan lyase variant has EC 4.2.2.12 activity.

55. The detergent composition of any of paragraphs 1-54, wherein saidxanthan lyase variant has an improved stability in a detergentcomposition compared to a parent xanthan lyase (e.g. with SEQ ID NO:6);optionally said detergent composition comprises a chelator; optionallysaid chelator is EDTA or citrate.

56. The detergent composition of any of paragraphs 1-55, wherein saidxanthan lyase variant has a half-life improvement factor (HIF) of 1.0;preferably said variant has a half-life improvement factor (HIF)of >1.0, more preferably at least 1.2, such as at least 1.5, e.g. atleast 2.0, relative to a parent xanthan lyase, e.g. a xanthan lyase withSEQ ID NO:6.

57. The detergent composition of paragraph 56, wherein said half-lifeimprovement factor (HIF) is determined after incubation of said xanthanlyase variant in a detergent composition at 25° C. for a time periodfrom about 30 min to about 20 h.

58. The detergent composition of any of paragraphs 1-57, wherein saidvariant is selected from the group consisting of i) the xanthan lyasevariants set forth in Table 17-33 herein and/or xii) the endoglucanasevariants set forth in any one of Tables 2-16 herein.

59. The detergent composition of any of paragraphs 1-58, wherein theendoglucanase variant has an alteration (e.g. a substitution, deletionor insertion, preferably a substitution) at one or more positions in:(i) regions 6 and 17; (ii) regions 6, 15 and 17; (iii) regions 10, 12and 15; (iv) regions 6, 7, 16, and 17; (v) region 6, 9, 10, 12, 15, and17; (vi) region 14 and 15; (vii) region 9; (viii) 6, 7, 9, 14, 15, 16,and 17; or (ix) 3, 6, 7, 9, 14, 15, 16, and 17; wherein said variantpreferably has no alternation in the other regions besides thosementioned.

60. The detergent composition of any of paragraphs 1-59, wherein theendoglucanase variant has an alteration (e.g. a substitution, deletionor insertion, preferably a substitution) in the positions selected fromthe group consisting of positions: 559+579+697; 512+559+579+697;18+71+186+408+579+602+651+688+756;18+189+408+559+579+688+697+756+921+934; 313+488; 880+905+921+934;302+313+408+579+602+651+697+880+921+934; or216+313+408+476+579+602+638+651+697+719+880+887+921+934 of SEQ ID NO:2.

61. The detergent composition of paragraph 60, wherein the endoglucanasevariant has an alteration (e.g. a substitution, deletion or insertion,preferably a substitution) selected from the group consisting of thefollowing alterations: A559N+Y579W+T697G; K512P+A559N+Y579W+T697G;N18G+A71E+A186P+E408D+Y579W+1602T+A651P+A688G+V756Y;N18G+N189K+E408D+A559N+Y579W+A688G+T697G+V756Y+K921R+Y934G; S313D+E408D;R880K+N905D+K921R+Y934G;I302D+S313D+E408D+Y579W+I602T+A651P+T697G+R880K+K921R+Y934G; andN216Q+5313D+E408D+D476R+Y579W+I602T+F638N+A651P+T697G+W719R+R880K+T887K+K921R+Y934G of SEQ ID NO:2.

62. The detergent composition of any one of paragraphs 1 to 61, whereinthe xanthan lyase variant comprises an alteration (e.g. a substitution,deletion or insertion, preferably a substitution) at one or morepositions in: (i) regions 3 and 5; (ii) regions 3, 5 and 12; (iii)regions 8, and 9; (iv) regions 2, 3, and 5; (v) regions 2, 3, 5, and 12;(vi) regions 3, 5, 8, 9, and 12; (vii) regions 2, 3, 5, 8, and 9; (viii)3, 5, 8, 9, and 12; (ix) 2, 3, 5, 8, 9, and 12; (x) region 3; (xi)regions 3, 4 and 5; (xii) regions 7, 8 and 9; (xiii) regions 12 and 13;(xiv) regions 3, 4, 5, 8, 9, and 12; (xv) regions 8, 9, 12, and 13;(xvi) regions 7, 8, 9, 12, and 13; (xvii) regions 3, 4, 5, 7, 8, 9, and12; and (xviii) regions 3, 4, 5, 7, 8, 9, 12, and 13, wherein saidvariant preferably has no alteration in the other regions besides thosementioned.

63. The detergent composition of any one of paragraphs 1 to 62, whereinthe xanthan lyase variant has an alteration (e.g. a substitution,deletion or insertion, preferably a substitution) in the positionsselected from the group consisting of positions: 190, 229, 234, 440,582, 624, 631, 635, 672, 703, 738, 752, 753, 754, 757, 769, 775, 801,875, 892, and any combination thereof, preferably229+672+752+753+769+775+801+875+892;229+672+753+754+769+775+801+875+892;229+672+752+753+754+769+775+801+875+892;190+229+234+624+672+753+754+769+775+801+875;190+229+631+672+703+752+753+769+775+801+875;190+229+234+582+672+753+754+757+769+775+801+875+892;229+440+582+624+635+672+738+753+754+757+769+775+801+875+892; or100+229+360+458+582+672+753+754+757+769+775+801+843+875+892+1008 of SEQID NO:6.

64. The detergent composition of any one of paragraphs 1 to 63, whereinthe xanthan lyase variant has an alteration (e.g. a substitution,deletion or insertion, preferably a substitution) selected from thegroup consisting of the following alterations:E229N+N672D+P752K+G753E+A769D+L775A+D801G+K875T+N892Y;E2295+N672D+G753E+5754E+A769D+L775A+D801G+K875T+N892Y;E2295+N672D+P752R+G753E+5754E+A769D+L775A+D801G+K875T+N892Y;A190Q+E229S+1234V+A624E+N672D+G753E+S754E+A769D+L775A+D801G+K875T;A190Q+E229S+T631N+N672D+1703L+P752K+G753E+A769D+L775A+D801G+K875T;A190Q+E229S+1234V+S582K+N672D+G753E+S754E+S757D+A769D+L775A+D801G+K875T+N892Y;E229S+N440K+S582K+A624E+S635E+N672D+G738L+G753E+S754E+S757D+A769D+L775A+D801G+K875T+N892Y; orS100D+E229S+K360G+D458S+S582K+N672D+G753E+S754E+S757D+A769D+L775A+D801G+A843P+K875T+N892Y+N1008D of SEQ ID NO:6.

65. The detergent composition of any one of paragraphs 1 to 64,comprising an endoglucanase variant having at least 61%, 62%, 63%, 64%,65%, 66%, 67%, 68%, 69%, 70%, 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%,79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%,93%, 94%, 95%, 96%, 97%, or 98% sequence identity to SEQ ID NO:2 andhaving an alteration (e.g. a substitution, deletion or insertion,preferably a substitution) in the positions selected from the groupconsisting of positions: 559+579+697; 512+559+579+697;18+71+186+408+579+602+651+688+756;18+189+408+559+579+688+697+756+921+934; 313+488; 880+905+921+934;302+313+408+579+602+651+697+880+921+934; or216+313+408+476+579+602+638+651+697+719+880+887+921+934 of SEQ ID NO:2,preferably having an alteration selected from the group consisting ofthe following alterations: A559N+Y579W+T697G; K512P+A559N+Y579W+T697G;N18G+A71E+A186P+E408D+Y579W+I602T+A651P+A688G+V756Y;N18G+N189K+E408D+A559N+Y579W+A688G+T697G+V756Y+K921R+Y934G; S313D+E408D;R880K+N905D+K921R+Y934G;I302D+S313D+E408D+Y579W+I602T+A651P+T697G+R880K+K921R+Y934G; andN216Q+5313D+E408D+D476R+Y579W+I602T+F638N+A651P+T697G+W719R+R880K+T887K+K921R+Y934G of SEQ ID NO:2, the endoglucanase variant preferably havingbesides the aforementioned alterations no further alterations relativeto the parent enzyme of SEQ ID NO:2.

66. The detergent composition of any one of paragraphs 1 to 65,comprising a xanthan lyase variant having at least 61%, 62%, 63%, 64%,65%, 66%, 67%, 68%, 69%, 70%, 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%,79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%,93%, 94%, 95%, 96%, 97%, or 98% sequence identity to SEQ ID NO:6 andhaving an alteration (e.g. a substitution, deletion or insertion,preferably a substitution) in the positions selected from the groupconsisting of positions: 229+672+752+753+769+775+801+875+892;229+672+753+754+769+775+801+875+892;229+672+752+753+754+769+775+801+875+892;190+229+234+624+672+753+754+769+775+801+875;190+229+631+672+703+752+753+769+775+801+875;190+229+234+582+672+753+754+757+769+775+801+875+892;229+440+582+624+635+672+738+753+754+757+769+775+801+875+892; or100+229+360+458+582+672+753+754+757+769+775+801+843+875+892+1008 of SEQID NO:6, preferably having an alteration selected from the groupconsisting of the following alterations:E229N+N672D+P752K+G753E+A769D+L775A+D801G+K875T+N892Y;E2295+N672D+G753E+S754E+A769D+L775A+D801G+K875T+N892Y;E2295+N672D+P752R+G753E+S754E+A769D+L775A+D801G+K875T+N892Y;A190Q+E229S+I234V+A624E+N672D+G753E+S754E+A769D+L775A+D801G+K875T;A190Q+E229S+T631N+N672D+1703L+P752K+G753E+A769D+L775A+D801G+K875T;A190Q+E229S+I234V+S582K+N672D+G753E+S754E+S757D+A769D+L775A+D801G+K875T+N892Y;E229S+N440K+S582K+A624E+S635E+N672D+G738L+G753E+S754E+S757D+A769D+L775A+D801G+K875T+N892Y; orS100D+E229S+K360G+D458S+S582K+N672D+G753E+S754E+S757D+A769D+L775A+D801G+A843P+K875T+N892Y+N1008D of SEQ ID NO:6, the xanthan lyase variantpreferably having besides the afore-mentioned alterations no furtheralterations relative to the parent enzyme of SEQ ID NO:6.

67. The detergent composition of any one of paragraphs 1 to 66,comprising

(A) an endoglucanase variant selected from those having at least 61%,62%, 63%, 64%, 65%, 66%, 67%, 68%, 69%, 70%, 71%, 72%, 73%, 74%, 75%,76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%,90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, or 98% sequence identity to SEQID NO:2 and having an alteration (e.g. a substitution, deletion orinsertion, preferably a substitution) in the positions selected from thegroup consisting of positions: 559+579+697; 512+559+579+697;18+71+186+408+579+602+651+688+756;18+189+408+559+579+688+697+756+921+934; 313+488; 880+905+921+934;302+313+408+579+602+651+697+880+921+934; or216+313+408+476+579+602+638+651+697+719+880+887+921+934 of SEQ ID NO:2,preferably having an alteration selected from the group consisting ofthe following alterations: (A1) A559N+Y579W+T697G; (A2)K512P+A559N+Y579W+T697G; (A3)N18G+A71E+A186P+E408D+Y579W+I602T+A651P+A688G+V756Y; (A4)N18G+N189K+E408D+A559N+Y579W+A688G+T697G+V756Y+K921R+Y934G; (A5)S313D+E408D; (A6) R880K+N905D+K921R+Y934G; (A7)I302D+S313D+E408D+Y579W+I602T+A651P+T697G+R880K+K921R+Y934G; and (A8)N216Q+S313D+E408D+D476R+Y579W+I602T+F638N+A651P+T697G+W719R+R880K+T887K+K921R+Y934G of SEQ ID NO:2; and

(B) a xanthan lyase variant having at least 61%, 62%, 63%, 64%, 65%,66%, 67%, 68%, 69%, 70%, 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%,80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%,94%, 95%, 96%, 97%, or 98% sequence identity to SEQ ID NO:6 and havingan alteration (e.g. a substitution, deletion or insertion, preferably asubstitution) in the positions selected from the group consisting ofpositions: 229+672+752+753+769+775+801+875+892;229+672+753+754+769+775+801+875+892;229+672+752+753+754+769+775+801+875+892;190+229+234+624+672+753+754+769+775+801+875;190+229+631+672+703+752+753+769+775+801+875;190+229+234+582+672+753+754+757+769+775+801+875+892;229+440+582+624+635+672+738+753+754+757+769+775+801+875+892; or100+229+360+458+582+672+753+754+757+769+775+801+843+875+892+1008 of SEQID NO:6, preferably having an alteration selected from the groupconsisting of the following alterations: (B1)E229N+N672D+P752K+G753E+A769D+L775A+D801G+K875T+N892Y; (B2)E229S+N672D+G753E+S754E+A769D+L775A+D801G+K875T+N892Y; (B3)E229S+N672D+P752R+G753E+S754E+A769D+L775A+D801G+K875T+N892Y; (B4)A190Q+E229S+I234V+A624E+N672D+G753E+S754E+A769D+L775A+D801G+K875T; (B5)A190Q+E229S+T631N+N672D+1703L+P752K+G753E+A769D+L775A+D801G+K875T; (B6)A190Q+E229S+I234V+S582K+N672D+G753E+S754E+S757D+A769D+L775A+D801G+K875T+N892Y; (B7)E229S+N440K+S582K+A624E+S635E+N672D+G738L+G753E+S754E+S757D+A769D+L775A+D801G+K875T+N892Y; and (B8)S100D+E229S+K360G+D458S+S582K+N672D+G753E+S754E+S757D+A769D+L775A+D801G+A843P+K875T+N892Y+N1008D of SEQ ID NO:6.

68. The detergent composition of paragraph 67, wherein the endoglucanaseand/or the xanthan lyase variant, preferably both, do not comprise anyfurther substitution besides those listed in paragraph 67.

69. The detergent composition of any one of paragraphs 1 to 68,comprising the endoglucanase variant Al and any one of the xanthan lyasevariants B1, B2, B3, B4, B5, B6, B7 and B8, wherein the respectiveenzymes preferably have at least 90%, preferably at least 91%, 92%, 93%,94%, 95%, 96% or 97% sequence identity to SEQ ID NO:2 and SEQ ID NO:6,respectively, more preferably are identical to their respective parentsequence with the exception of the substitutions explicitly listed.

70. The detergent composition of any one of paragraphs 1 to 68,comprising the endoglucanase variant A2 and any one of the xanthan lyasevariants B1, B2, B3, B4, B5, B6, B7 and B8, wherein the respectiveenzymes preferably have at least 90%, preferably at least 91%, 92%, 93%,94%, 95%, 96% or 97% sequence identity to SEQ ID NO:2 and SEQ ID NO:6,respectively, more preferably are identical to their respective parentsequence with the exception of the substitutions explicitly listed.

71. The detergent composition of any one of paragraphs 1 to 68,comprising the endoglucanase variant A3 and any one of the xanthan lyasevariants B1, B2, B3, B4, B5, B6, B7 and B8, wherein the respectiveenzymes preferably have at least 90%, preferably at least 91%, 92%, 93%,94%, 95%, 96% or 97% sequence identity to SEQ ID NO:2 and SEQ ID NO:6,respectively, more preferably are identical to their respective parentsequence with the exception of the substitutions explicitly listed.

72. The detergent composition of any one of paragraphs 1 to 68,comprising the endoglucanase variant A4 and any one of the xanthan lyasevariants B1, B2, B3, B4, B5, B6, B7 and B8, wherein the respectiveenzymes preferably have at least 90%, preferably at least 91%, 92%, 93%,94%, 95%, 96% or 97% sequence identity to SEQ ID NO:2 and SEQ ID NO:6,respectively, more preferably are identical to their respective parentsequence with the exception of the substitutions explicitly listed.

73. The detergent composition of any one of paragraphs 1 to 68,comprising the endoglucanase variant A5 and any one of the xanthan lyasevariants B1, B2, B3, B4, B5, B6, B7 and B8, wherein the respectiveenzymes preferably have at least 90%, preferably at least 91%, 92%, 93%,94%, 95%, 96% or 97% sequence identity to SEQ ID NO:2 and SEQ ID NO:6,respectively, more preferably are identical to their respective parentsequence with the exception of the substitutions explicitly listed.

74. The detergent composition of any one of paragraphs 1 to 68,comprising the endoglucanase variant A6 and any one of the xanthan lyasevariants B1, B2, B3, B4, B5, B6, B7 and B8, wherein the respectiveenzymes preferably have at least 90%, preferably at least 91%, 92%, 93%,94%, 95%, 96% or 97% sequence identity to SEQ ID NO:2 and SEQ ID NO:6,respectively, more preferably are identical to their respective parentsequence with the exception of the substitutions explicitly listed.

75. The detergent composition of any one of paragraphs 1 to 68,comprising the endoglucanase variant A7 and any one of the xanthan lyasevariants B1, B2, B3, B4, B5, B6, B7 and B8, wherein the respectiveenzymes preferably have at least 90%, preferably at least 91%, 92%, 93%,94%, 95%, 96% or 97% sequence identity to SEQ ID NO:2 and SEQ ID NO:6,respectively, more preferably are identical to their respective parentsequence with the exception of the substitutions explicitly listed.

76. The detergent composition of any one of paragraphs 1 to 68,comprising the endoglucanase variant A8 and any one of the xanthan lyasevariants B1, B2, B3, B4, B5, B6, B7 and B8, wherein the respectiveenzymes preferably have at least 90%, preferably at least 91%, 92%, 93%,94%, 95%, 96% or 97% sequence identity to SEQ ID NO:2 and SEQ ID NO:6,respectively, more preferably are identical to their respective parentsequence with the exception of the substitutions explicitly listed.

77. The detergent composition of paragraph 67 or 68, comprising (1) theendoglucanase variant A1 and the xanthan lyase variant B1; (2) theendoglucanase variant A1 and the xanthan lyase variant B2; (3) theendoglucanase variant A1 and the xanthan lyase variant B3; (4) theendoglucanase variant A1 and the xanthan lyase variant B4; (5) theendoglucanase variant A1 and the xanthan lyase variant B5; (6) theendoglucanase variant A1 and the xanthan lyase variant B6; (7) theendoglucanase variant A1 and the xanthan lyase variant B7; (8) theendoglucanase variant A1 and the xanthan lyase variant B8; (9) theendoglucanase variant A2 and the xanthan lyase variant B1; (10) theendoglucanase variant A2 and the xanthan lyase variant B2; (11) theendoglucanase variant A2 and the xanthan lyase variant B3; (12) theendoglucanase variant A2 and the xanthan lyase variant B4; (13) theendoglucanase variant A2 and the xanthan lyase variant B5; (14) theendoglucanase variant A2 and the xanthan lyase variant B6; (15) theendoglucanase variant A2 and the xanthan lyase variant B7; (16) theendoglucanase variant A2 and the xanthan lyase variant B8; (17) theendoglucanase variant A3 and the xanthan lyase variant Bl; (18) theendoglucanase variant A3 and the xanthan lyase variant B2; (19) theendoglucanase variant A3 and the xanthan lyase variant B3; (20) theendoglucanase variant A3 and the xanthan lyase variant B4; (21) theendoglucanase variant A3 and the xanthan lyase variant B5; (22) theendoglucanase variant A3 and the xanthan lyase variant B6; (23) theendoglucanase variant A3 and the xanthan lyase variant B7; (24) theendoglucanase variant A3 and the xanthan lyase variant B8; (25) theendoglucanase variant A4 and the xanthan lyase variant Bl; (26) theendoglucanase variant A4 and the xanthan lyase variant B2; (27) theendoglucanase variant A4 and the xanthan lyase variant B3; (28) theendoglucanase variant A4 and the xanthan lyase variant B4; (29) theendoglucanase variant A4 and the xanthan lyase variant B5; (30) theendoglucanase variant A4 and the xanthan lyase variant B6; (31) theendoglucanase variant A4 and the xanthan lyase variant B7; (32) theendoglucanase variant A4 and the xanthan lyase variant B8; (33) theendoglucanase variant A5 and the xanthan lyase variant B1; (34) theendoglucanase variant A5 and the xanthan lyase variant B2; (35) theendoglucanase variant A5 and the xanthan lyase variant B3; (36) theendoglucanase variant A5 and the xanthan lyase variant B4; (37) theendoglucanase variant A5 and the xanthan lyase variant B5; (38) theendoglucanase variant A5 and the xanthan lyase variant B6; (39) theendoglucanase variant A5 and the xanthan lyase variant B7; (40) theendoglucanase variant A5 and the xanthan lyase variant B8; (41) theendoglucanase variant A6 and the xanthan lyase variant B1; (42) theendoglucanase variant A6 and the xanthan lyase variant B2; (43) theendoglucanase variant A6 and the xanthan lyase variant B3; (44) theendoglucanase variant A6 and the xanthan lyase variant B4; (45) theendoglucanase variant A6 and the xanthan lyase variant B5; (46) theendoglucanase variant A6 and the xanthan lyase variant B6; (47) theendoglucanase variant A6 and the xanthan lyase variant B7; (48) theendoglucanase variant A6 and the xanthan lyase variant B8; (49) theendoglucanase variant A7 and the xanthan lyase variant B1; (50) theendoglucanase variant A7 and the xanthan lyase variant B2; (51) theendoglucanase variant A7 and the xanthan lyase variant B3; (52) theendoglucanase variant A7 and the xanthan lyase variant B4; (53) theendoglucanase variant A7 and the xanthan lyase variant B5; (54) theendoglucanase variant A7 and the xanthan lyase variant B6; (55) theendoglucanase variant A7 and the xanthan lyase variant B7; (56) theendoglucanase variant A7 and the xanthan lyase variant B8; (57) theendoglucanase variant A8 and the xanthan lyase variant B1; (58) theendoglucanase variant A8 and the xanthan lyase variant B2; (59) theendoglucanase variant A8 and the xanthan lyase variant B3; (60) theendoglucanase variant A8 and the xanthan lyase variant B4; (61) theendoglucanase variant A8 and the xanthan lyase variant B5; (62) theendoglucanase variant A8 and the xanthan lyase variant B6; (63) theendoglucanase variant A8 and the xanthan lyase variant B7; (64) theendoglucanase variant A8 and the xanthan lyase variant B8; or (65) theendoglucanase variant and xanthan lyase variant combinations disclosedin Talbes 34-36.

78. The detergent composition of any of paragraphs 1-77, furthercomprising one or more detergent components.

79. The detergent composition of any of paragraphs 1-78, furthercomprising one or more additional enzymes selected from the groupconsisting of: endoglucanases, proteases, amylases, lichenases, lipases,cutinases, cellulases, xanthan lyases, xyloglucanases, pectinases,pectin lyases, xanthanases, peroxidases, haloperoxygenases, catalasesand mannanases, or any mixture thereof.

80. The detergent composition of any of paragraphs 1-79, wherein saidcomposition is in form of a bar, a homogenous tablet, a tablet havingtwo or more layers, a pouch having one or more compartments, a regularor compact powder, a granule, a paste, a gel, or a regular, compact orconcentrated liquid.

81. Use of a detergent composition of any of paragraphs 1-80, whereinsaid use is for degrading xanthan gum.

82. The use of paragraph 81, wherein said endoglucanase variant and/orxanthan lyase variant has an enzyme detergency benefit.

83. A method for degrading xanthan gum comprising: applying a detergentcomposition of any of paragraphs 1-80 to a xanthan gum.

84. The method of paragraph 83, wherein said xanthan gum is on a surfaceor hard surface.

The present invention is further described by the following examplesthat should not be construed as limiting the scope of the invention.

EXAMPLES Example 1 Construction of GH9 Endoglucanase Variants of theMature Parent Eendoglucanase Having SEQ ID NO:2

A linear integration vector-system was used for cloning of the matureparent nucleotide sequence having SEQ ID NO:1 (same also disclosed asmature peptide within SEQ ID NO:1 of WO2013/167581) coding for themature parent polypeptide of the GH9 endoglucanase of SEQ ID NO:2, andits variants. The linear integration construct was a PCR fusion made byfusing the gene between two Bacillus subtilis homologous chromosomalregions along with strong promoters and a chloramphenicol resistancemarker. The fusion was made by Splicing by Overlap Extension (SOE) PCR(Horton et al. (1989) Engineering hybrid genes without the use ofrestriction enzymes, or gene splicing were produced by overlap extension(Gene 77: 61-68). The SOE PCR method is also described in patentapplication WO2003/095658. The gene was expressed under the control of atriple promoter system (as described in WO99/43835), consisting of thepromoters from Bacillus licheniformis alpha-amylase gene (amyL),Bacillus amyloliquefaciens alpha-amylase gene (amyQ), and the Bacillusthuringiensis cryIIIA promoter including stabilizing sequence. The genecoding for chloramphenicol acetyltransferase was used as marker(described in e.g. Diderichsen et al. (1993) A useful cloning vector forBacillus subtilis. Plasmid 30:312). The final gene constructs wereintegrated on the Bacillus chromosome by homologous recombination intothe pectate lyase locus. The gene fragments were amplified fromchromosomal DNA of the corresponding strains with gene specific primerscontaining overhang to the two flanking vector fragments. All genes wereexpressed with a Bacillus licheniformis alpha-amylase secretion signalhaving the nucleotide sequence of SEQ ID NO:3 and the amino acidsequence of SEQ ID NO:4 replacing the native secretion signal.

Variants of the mature parent GH9 endocluconase from Paenibacillussp-62047 having SEQ ID NO:2 as described in Examples 3-4 below were madeby the megaprimer mutagenesis method using specifically designedmutagenic oligonucleotides introducing desired mutations in theresulting sequence. Design and production methods for such mutagenicoligonucleotides introducing desired mutations into target sequences arewell known to those skilled in the art. Consequently, mutagenic oligoswere designed and synthesized corresponding to the DNA sequence flankingthe desired site(s) of mutation, separated by the DNA base pairsdefining the substitutions. The final expression cassette composed thereference GH9 endocluconase from Paenibacillus sp-62047 as describedabove (i.e. parent GH9 endocluconase having SEQ ID NO:1). Sucessfulintroduction of the desired substitutions was confirmed by DNAsequencing of the GH9 endogluconase gene. An aliquot of the PCR productwas subsequently transformed into Bacillus subtilis. Transformants wereselected on LB agar plates supplemented with 10 mM K₂PO₄, 0.4% extraglucose and 6 μg of chloramphenicol per mL. The resulting recombinantBacillus subtilis clone containing the integrated expression constructwas grown in liquid culture as described below. The enzyme containingsupernatants were harvested and the enzymes (variants) were eitherstress tested using a reducing sugar assay or purified as describedbelow.

Variants above were produced by fermentation using standard protocols(TB-glycerol media containing a standard trace metal mix as described inF. William Studier (2005) Protein production by auto-induction inhigh-density shaking cultures, Protein Expression and Purification, 41:207-234) and grown for 4 days at 30° C. before harvested). Supernatantsof samples used for stress testing were inoculated from an overnightculture grown at 37° C. and subsequently fermented in 96-well plateformat (TB-glycerol media described above without calcium in the tracemetal mix for 4 days 30° C.).

Example 2 Purification of GH9 Endoglucanase Variants

The culture broth was centrifuged at 13'000 rpm (45 min, 18° C.,F125-6×500 rotor) using a Sorval RC-6 plus centrifuge (ThermoFisherScientific). The supernatant was supplemented with (NH₄)₂SO₄ to a finalconcentration of 0.8 M. The mixture was filtered using 0.2 μm bottle-toprapid flow filters (Nalgene). The mixture was loaded on a 50 mL PhenylSepharose High Performance (GE Healthcare, Uppsala, Sweden)pre-equilibrated with 20 mM Tris-HCl, pH 8.0 with 0.8 M (NH₄)₂SO₄.Flowrate was set to 3 mL/min. After protein loading, the flow rate wasincreased to 5 mL/min and unbound or loosely bound protein was washedout by several column volumes of equilibration buffer. Elution wascarried out by step-wise increase of elution buffer (20 mM Tris-HCl, pH8.0). The target protein eluted during the (75-100%) elution step.Fractions of 8 mL were collected during the purification. The fractionswere evaluated using SDS-PAGE (NuPAGE, Invitrogen). Fractions elutingwith 20 mM Tris-HCl, pH 8.0 were pooled and desalted on a 350 mL G25desalting column pre-equilibrated with 20 mM Tris-HCl, pH 8.5. Thedesalted protein solution was applied on a 20 mL Source15Q columnpre-equilibrated with 20 mM Tris-HCl, pH 8.5 at 2 mL/min. Unbound orloosely bound proteins were washed using at least two column volumes ofequilibration buffer until a stable UV baseline was obtained. The flowrate was raised to 4 mL/min and elution was done by a linear NaClgradient using the elution buffer (20 mM Tris-HCl, pH 8.5 +750 mM NaCl).3 mL fractions were collected during the purification. SDS-PAGE was usedto evaluate the fractions. Pure fractions were pooled and concentratedif necessary using Vivaspin 20 (10 kDa Cut-off, Sartorius). Proteinconcentration was determined using absorbance measurements at 280 nm.

Example 3 Detergent Stability Assay

GH9 endoglucanase (EG) activity (EC 3.2.1.4) was determined by reducingends on xanthan gum pre-treated with xanthan lyase using thecolorimetric assay developed by Lever (1972), Anal. Biochem. 47:273-279, 1972. Pre-treated xanthan gum is a modified form of the xanthansugar, where the terminal pyruvated mannose from side chains is removed(prepared according to Nankai et al. (1999) from the source Keltran).The GH9 mature parent endoglucanase and its variants cleave atbeta-(1,4)-glucosyl bonds in the glucan backbone of pretreated xanthangum releasing glucans with a reducing end which can be determined byreaction with p-Hydroxybenzoic acid hydrazide (PAHBAH). The increase ofcolour is proportional to the enzyme activity under the conditions usedin the assay (e.g. Table 1) and used to estimate the residual activity(RA), half-life (T1/2) and the half-life improvement factor (HIF).

TABLE 1 Description of assays Stress assay: Detergent Persil UniversalGel Assay buffer (AB) 50 mM MOPS, 4 mM CaCl₂, 0.01% Triton X-100, pH 7.0Reference sample conditions 4° C. for 5-138 h Stress conditions 25, 26,28 or 30° C. for 5-138 h Activity assay: Substrate concentration 4 mg/mLmodified xanthan gum Xanthan gum incubation 50° C. for 1 h PAHBAHsolution 15 mg/mL 4-hydroxybenzoic acid hydrazide (PAHBAH), 50 g/Lpotassium sodium tartrate tetrahydrate, 20 g/L NaOH PAHBAH development95° C. for 10 min

Method Steps:

30 μL enzyme sample (purified variant, 10-150 ppm) was mixed with 270 μLdetergent using magnetic stirring for 15 minutes in a micro titer plate(MTP). This plate was designated as the “stress MTP”. 20 μL of themixture was transferred to a new MTP and diluted 100-fold using a 2-stepdilution (2×10-fold dilution). The sample was diluted into assay buffer(AB): 50 mM MOPS, 4 mM CaCl₂, 0.01% Triton X-100, pH 7.0. This dilutedMTP is the “reference MTP” and is stored at 4° C. for 5-138 h (ata timeinterval equal to that of the stress MTP below). The stress MTP wasincubated at 25, 26, 28 or 30° C. for 5-138 h. After incubation, thestress MTP was initially mixed by magnetic stirring for 15 min, and thestress MTP was then diluted 100-fold as described for the reference MTP.To assess the enzymatic activity, 50 μL of diluted enzyme:detergentsample (from both reference and stress MTPs) was mixed with 50 μL 4mg/mL modified xanthan gum in PCR plates. The samples were thenincubated at 50° C. for 1 h. Finally, the level of reducing ends wasestimated by adding 75 μL PAHBAH solution (15 mg/mL PAHBAH, 50 g/Lpotassium sodium tartrate tetrahydrate, 20 g/L NaOH) to all samples inthe PCR plates. The samples were then incubated at 95° C. for 10 min.After cooling down to room temperature, the absorbance at 405 nm wasmeasured.The residual activity (RA) was calculated using the followingformula:

RA (%)=(Abs(Stress))/(Abs(Ref))×100%

Abs(Stress): The absorbance at 405 nm of the sample in the stress MTP(incubated at 25, 26, 28 or 30° C.) after subtracting relevantbackground absorbance contributions.

Abs(Ref): The absorbance at 405 nm of the sample in the reference MTP(incubated at 4° C.).

-   -   Also, the half-lives for the degradation of each variant and        parent endoglucanase were    -   calculated using the following formula (by applying 1st order        kinetics for the degradation

of EG):

${T{1/2}} = {- \frac{{\ln (2)} \times T}{\ln \left( \frac{{Abs}({Stress})}{{Abs}({Ref})} \right)}}$

-   -   T: The incubation time. Abs(Stress) and Abs(Ref): See above.

Half-life improvement factors (HIFs) can then be calculated as:

${H\; I\; F} = \frac{{T{1/2}},{variant}}{{T{1/2}},{wt}}$

-   -   T½, variant: The half-life for a specific variant    -   T½, wt (or wild-type): The half-life for EG wt (EG wild type),        wherein said T½ wt is    -   T½ of the mature parent endoglucanase with SEQ ID NO:2.

The HIFs of the tested variants are shown in Tables 2-7 below: Allhalf-life values of the variants measured in culture supernatant werecalculated relative to the half-life of GH9 wild-type (mature parentendoclucanase with SEQ ID NO:2) measured as culture supernatant. Allhalf-life values of the variants measured as purified protein werecalculated relative to the half-life of GH9 wild-type (mature parentendoclucanase with SEQ ID NO:2) measured as purified protein. HIFs ofall variants were calculated based on the wild-type endoglucanase (SEQID NO:2) incubated at the same detergent concentration and temperature(HIF of wild-type=1 in all tables, per definition).

TABLE 2 Variants of the mature parent GH9 endoglucanase (SEQ ID NO: 2)with corresponding half-life improvement factors (HIF) measured asculture supernatants Regions (as defined herein) Alteration HIF 4 N285G1.4 5 W333L 1.2 5 T353D 1.8 6 N558F 1.4 7 T633V 1.1 7 D635L 2.4 7 D635M1.2 7 D635T 1.1 7 F638Y 1.1 7 T639D 1.2 9 G994N 3.7

TABLE 3 Variants of the mature parent GH9 endoglucanase (SEQ ID NO: 2)with corresponding half-life improvement factors (HIF) measured asculture supernatants Regions (as defined herein) Alteration HIF 4 K281T1.3 6 N558NP >10.0 6 G563E 1.14 6 I575M >10.0 6 I575A 1.3 9 K921D 1.4

TABLE 4 Variants of the mature parent GH9 endoglucanase (SEQ ID NO: 2)with corresponding half-life improvement factors (HIF) measured asculture supernatants Regions (as defined herein) Alteration HIF 2 I125V1.1 2 A126R 1.1 2 K130R 1.1 3 K213R 1.2 3 A221R 1.1 3 K228E 1.1 3 K228I1.2 3 G230F 1.2 3 G230L 1.2 3 G230A 1.2 3 G230H 1.2 3 G230N 1.1 3 G230W1.1 3 G230T 1.2 3 F231Y 1.2 3 F231N 1.1 3 V232R 1.1 3 V232G 1.1 3 H235D1.1 3 N240Q 1.2 3 G243K 1.1 3 G243R 1.2 3 A249N 1.1 4 A278S 1.1 4 K281F1.1 4 K281V 1.2 4 K281Y 1.1 4 K281H 1.1 4 K281Q 1.1 4 K281N 1.2 4 K281W1.1 4 N285L 1.9 4 N285M 1.8 4 N285S 1.2 4 N285P 1.6 4 N285T 1.1 4 N285Y1.8 4 N285H 2.4 4 N285K 1.4 4 N285D 1.6 4 N285W 1.3 4 N285R 1.5 4 T292F1.2 4 T292L 2 4 T292I 1.5 4 T292V 1.5 4 T292S 1.3 4 T292P 1.6 4 T292Y1.2 4 T292Q 1.4 4 T292N 1.1 4 T292K 1.2 4 T292D 1.3 4 T292G 1.4 4 F297L1.1 5 A346H 1.1 6 G556S 1.2 6 N558D 1.4 6 N558M 1.2 6 N558Q 1.3 6 N558I1.2 6 N558Y 1.1 6 N558H 1.1 6 A559N 1.7 6 A559F 1.2 6 A559M >10.0 6A559P 1.5 6 A559Y 1.3 6 A559H 1.6 6 A559Q 1.4 6 A559D 1.5 6 A559R 1.1 6A559G 1.1 6 A559I 1.1 6 A559S 1.2 6 S560P 1.3 6 S560K 1.2 6 S560G 1.4 6S560D 1.3 6 T561P 1.6 6 T561E 1.1 6 T561Q 1.1 6 T561S 1.1 6 T561D 1.2 6A564I 1.5 6 A564Y 1.2 6 A564H 1.1 6 A564Q 1.2 6 A564K 1.5 6 A564E 1.4 6E565M 1.2 6 V567F 1.1 6 K568R 1.3 6 L569F 1.2 6 L569Y 1.2 6 L569D 1.2 6L569E 1.3 6 P570F 1.3 6 P570L 1.6 6 P570I 1.8 6 P570M 2.1 6 P570V 2.6 6P570S 4.9 6 P570T 5.4 6 P570A 4.4 6 P570Y 2.1 6 P570H 2.6 6 P570Q 5.1 6P570N 3.3 6 P570K 4.2 6 P570E 1.6 6 P570W 1.7 6 P570R 3.6 6 P570G 1.4 6I575D 1.5 6 I575E 3.7 6 I576F 1.8 6 I576M 1.2 6 I576P 3.3 6 D578R 1.1 6Y579F 1.1 6 Y579W 2.1 6 V580L 1.2 6 D583M 1.1 6 Q589G 1.1 6 P590S 1.1 6P590T 1.1 6 P590E 1.2 6 E591L 1.5 6 G592D 1.1 6 S593P 1.4 6 S593H 1.3 6S593Q 1.3 6 S593N 1.5 6 S593K 1.2 6 S593D 1.3 6 S593E 1.3 6 S593R 1.3 7S616D 1.2 7 K627L 1.8 7 K627M 2.2 7 K627V 2.4 7 K627S 1.1 7 K627T 1.7 7K627Q 2.5 7 K627R 4.3 7 I630F 1.4 7 I630V 1.2 7 I630Y 1.2 7 D635A 1.3 7D635P 1.1 7 D635N 1.3 7 D635K 1.3 7 D635E 1.4 7 D635G 1.1 7 D635W 1.1 7S636L 1.6 7 S636M 1.9 7 S636A 1.4 7 S636H 1.4 7 S636Q 2.4 7 S636N 2 7S636K 1.8 7 S636R 1.3 7 F638I 1.7 7 F638V 1.4 7 F638T 1.2 7 F638L 1.1 7F638H 1.3 7 T639V 1.1 7 T639S 1.1 7 T639L 1.2 7 T639I 2.1 7 T639M 1.2 7T639A 2.2 7 T639E 1.8 7 T639W 1.3 7 T639G 2.1 7 Y641E 1.2 7 S642T 2.1 7S642N 1.3 7 N643D 2.0 7 N643H 2.1 7 N643T 1.2 7 T644F 1.1 7 A651P 1.5 8S810R 1.2 8 A811S 1.1 8 V812F 1.3 8 V812I 1.2 8 V812M 1.3 8 V812W 1.2 8V812R 1.1 8 N815V 1.1 8 N815Y 1.1 8 N815E 1.2 8 N815W 1.1 8 N815R 1.1 8S823Q 1.1 8 A824T 1.1 8 T825N 1.1 8 T825W 1.1 8 T825A 1.2 8 T825D 1.1 8V827I 1.2 8 V827M 1.5 8 V827S 1.2 9 T843V 1.1 9 D870F 1.2 9 D870L 1.3 9D870I 1.3 9 D870M 1.4 9 D870V 1.5 9 D870S 1.3 9 D870T 1.1 9 D870Y 1.1 9D870H 1.2 9 D870Q 1.3 9 D870N 1.5 9 D870K 1.4 9 D870E 1.4 9 D870W 1.1 9D870R 1.2 9 D870G 1.2 9 P871F 1.3 9 P871L 1.3 9 P871I 1.5 9 P871M 1.3 9P871V 1.3 9 P871S 1.3 9 P871T 1.4 9 P871A 1.3 9 P871Y 1.2 9 P871H 1.3 9P871Q 1.5 9 T872S 1.1 9 T872F 1.1 9 T872A 1.1 9 T872Y 1.2 9 T872H 1.6 9T872Q 1.5 9 T872N 1.5 9 T872K 1.4 9 T872D 1.4 9 T872E 1.5 9 T872W 1.5 9T872R 1.7 9 T872G 1.3 9 D873K 1.1 9 D873E 1.2 9 T874V 1.2 9 T874S 1.3 9T874P 1.1 9 T874A 1.2 9 T874H 1.1 9 T874Q 1.1 9 T874N 1.2 9 T874K 1.3 9V881Q 1.2 9 T883K 1.3 9 Y884H 1.8 9 A885F 5.4 9 A885Q 1.3 9 A885N 1.1 9T887L 1.1 9 T887I 1.1 9 T887S 1.6 9 T887H 1.1 9 T887R 1.1 9 K894E 1.1 9N920D 1.3 9 K921R 2.4 9 K921E 1.2 9 T932A 1.5 9 N933V 1.4 9 N933S 2.5 9Y934G 4.8 9 Y934M 1.2 9 Y934S 3.8 9 Y934A 3.7 9 Y934Q 4.2 9 Y934N 2.8 9Y934E 2.3 9 Y934W 1.5 9 Y934R 6 9 T935W 1.1 9 A937F 1.1 9 A937V 1.7 9A937S 1.1 9 A937T 1.4 9 A937Q 1.1 9 A937D 1.4 9 A937E 2.6 9 V938I 1.8 9K939I 1.8 9 K939V 3.4 9 D940E 1.1 9 N941S 2.4 9 N941H 1.6 9 N941D 1.3 9A942P 1.7 9 A942E 1.2 9 D943Y 1.1 9 D943H 1.2 9 R950V 1.2 9 R950H 1.2 9R950N 1.4 9 F952S 1.2 9 F952W 1.1 9 N953Y 1.7 9 G954L 1.4 9 Y960F 1.4 9A964N 1.2 9 A964C 1.2 9 N966P 1.1 9 N966C 1.5 9 G971A 1.1 9 Q974K 1.1 9Q974C 1.1 9 Q989I 1.1 9 Q991L 1.1 9 Q991I 1.2 9 Q991M 1.1 9 Q991V 1.2 9Q991T 1.3 9 Q991K 1.1 9 Q991C 1.1 9 S995I 1.1 9 S995V 1.1 9 S995Q 1.1 9S995R 1.1 9 S995C 1.3 9 G998V 1.1 9 G998A 1.1 9 S1006T 1.2 9 S1006A 1.19 S1006K 1.1 9 S1006R 1.2 9 Y1010W 1.1 9 L1011M 1.2 9 L1011S 1.3 9L1011A 1.4 9 L1011Q 1.1 9 L1011N 1.1 9 L1011D 1.2 9 L1011E 1.2 9 R1029N1.2 9 F1030M 1.1 9 K1031I 1.2 9 K1031S 1.4 9 K1031T 2.1 9 K1031H 1.2 9V1032G 1.3 9 K1035A 1.2 9 A1037E 1.4 9 A1037W 1.6 9 S1038L 1.1 9 S1038I1.2 9 L1040N 1.3 9 L1040E 1.1 9 G1041F 1.1 9 L1044F 1.1 9 L1044S 1.1 9L1044N 1.1 9 L1044W 1.1 9 P1045Q 1.2 9 P1045W 1.2

TABLE 5 Variants of the mature parent GH9 endoglucanase (SEQ ID NO: 2)with corresponding half-life improvement factors (HIF) measured asculture supernatants Alteration HIF A559N + Y579F 2.8 A564E + Y579F 1.1A559N + Y579W 3.2 G562P + Y579W 1.4 A564D + Y579W 2.1 A559N + Y579W +K99R 3.8 A559N + Y579W + K281R 3.4 K281R + A559N + Y579W 3.5 A559N +Y579W + S616D 5.6 A559N + Y579W + S636N 9 A559N + Y579W + A651P 4.2A559N + Y579W + K948E 2.6 A559N + Y579W + K1009E 2.4 A559N + Y579W +K627R 7.8 Y579W + K921R 3.2 A559N + Y579W + K921R 6.1 K99R + Y579W 1.6Y579W + A651P 2.7 Y579W + K948E 1.4 Y579W + K1009E 1.5 A559N + Y579W +Y934G 16.0 A559N + Y579W + K921R + Y934G 29.5 A559N + Y579W + K627M 6.4A559N + Y579W + K627R + S616D 18.1 A559N + Y579F + K627R 4.4 A559N +Y579W + K921R + A651P 9.9 A559N + Y579W + K921R + K627R 17.6 A559N +Y579W + K921R + S636K 5.9 A559N + Y579W + K921R + S616D 4.3 A559N +Y579W + K921R + S636N 8.9 A559N + Y579W + K921R + K627R + S636N 26.7A559N + Y579W + S636N + A651P 9.6 A559N + Y579W + S616D + A651P 9.4A559N + Y579W + S616D + S636K 6.5 A559N + Y579W + S616D + K921R + Y934G19.9 A559N + Y579W + A651P + K627M 10.6 A559N + Y579W + A651P + S636K8.2 A559N + Y579W + A651P + K627R + S636N 37.0 A559N + Y579W + A651P +S616D 9.0 A559N + Y579W + A651P + K921R + Y934G 21.1 S636N + Y934G 1.6S636N + K921R 1.4 S636N + K627R 2.4 S636N + Y579W 1.6 F638I + Y934G 3.0F638I + K921R 1.4 F638I + K627R 2.2 F638I + Y579W 1.3 K627R + K51Q 1.3K627R + K451S 1.4 K627R + A559N 1.5 K627R + Y579W 3.9 Y579W + Y934G 4.1A651P + F638I 1.2 P570Q + A651P 2.7 P570Q + K921R 1.6 P570Q + K627R 5.1P570Q + A559N 1.8 P570Q + Y579W 3.4 P570Q + F638I 1.2 P570K + Y579W 2.6P570K + F638I 1.5 P570T + A651P 2.6 P570T + S636N 3.4 P570T + Y934G 4.3P570T + F638I 1.4 P570T + K921R 1.5 P570T + K627R 3.9 P570T + A559N 1.8P570T + A885F 1.2 A885F + Y934G 1.5 A885F + K627R 1.2 A559N + Y579W +S636L 1.3 A559N + Y579W + F638I 2.1 A559N + Y579W + D870M 1.1 A559N +Y579W + S560P 4.1 A559N + Y579W + A564I 1.3 A559N + Y579W + P570N 2.8A559N + Y579W + P570K 5.2 A559N + Y579W + P570R 5.4 A559N + Y579W +P570A 4.1 A559N + Y579W + P570T 3.8 A559N + Y579W + P570S 3.9 A559N +Y579W + P570Q 3.3 A559N + Y579W + P570H 3.7

TABLE 6 Variants of the mature parent GH9 endoglucanase (SEQ ID NO: 2)with corresponding half-life improvement factors (HIF) measured aspurified samples Regions (as defined herein) Alteration HIF 6 N558E 1.16 A559P 1.4 6 A559N 1.6 6 A559H 1.5 6 T561P 1.1 6 A564E 1.2 6 P570A 7.06 P570Q 7.0 6 P570R 7.0 6 P570S 7.0 6 P570K 6.7 6 P570T 6.3 6 P570N 2.96 Y579W 2.5 6 Y579F 1.3 6 T581M 1.2 7 S616D 1.4 7 K627R 2.1 7 K627M 2.17 K627Q 1.2 7 S636N 1.8 7 S636Q 1.5 7 S636R 1.4 7 S636K 1.4 7 S636M 1.37 S636H 1.1 7 F638I 1.6 7 F638L 1.2 7 N643D 1.5 7 A651P 1.3 7 A651S 1.29 A885F 1.1 9 A885Q 1.1 9 K921R 4.2 9 Y934R 14 9 Y934G 6.2 9 N966C 1.3 9L1011A 1.1 9 K1031I 1.3

TABLE 7 Variants of the mature parent GH9 endoglucanase (SEQ ID NO: 2)with corresponding half-life improvement factors (HIF) measured aspurified samples Alteration HIF N558K + A559K + S560F + T561P + G562W3.8 A559N + P570A + Y579W 4.6 A559N + P570H + Y579W 3.5 A559N + P570K +Y579W 10.2 A559N + P570N + Y579W 3.4 A559N + P570Q + Y579W 4.5 A559N +P570R + Y579W 5.5 A559N + P570S + Y579W 5.0 A559N + P570T + Y579W 6.0A559N + S560P + Y579W 2.9 A559N + Y579W + A651P 1.6 A559N + Y579W +A651P + Y934G 6.5 A559N + Y579W + F638I 1.6 A559N + Y579W + K921R 1.4A559N + Y579W + S616D + K921R 7.4 A559N + Y579W + S636N 1.8 A559N +Y579F 1.7 A559N + Y579W 4.2 A559N + Y579W + K921R 5.8 A559N + Y579W +S616D 5.7 F638I + Y934G 1.8 K627R + S636N 1.5 K627R + Y934G 2.8 P570K +Y579W 3.1 Q416D + A559N + Y579W + S636N 3.9 S128X + A559N + Y579W +K627R 12.7 S128X + A559N + Y579W + S636N 7.6

Example 4 Half-Life Improvement Factors (HIF) of Endoglucanase Vvariantswith Mutations in Chelator-Induced Instability Regions and AdjacentRegions

Variants of the mature parent endoglucanase of SEQ ID NO:2 were preparedand purified as described above in Examples 1 and 2. For the purposes ofthis example, variants were produced having mutations in at least onechelator-induced instability region (regions 1, 2, 3, 4, 5, 6, 7, 8, 9)and/or in at least one adjacent region (regions 10, 11, 12, 13, 14, 15,16, 17, 18, 19). The in-detergent stability of the variants wasdetermined as described in Example 3 by measuring the enzymatic activitypresent in purified samples of the variants after incubation withdetergent and a protease. Incubation was performed using a 90% or 95%concentration of Persil Universal Gel detergent (PUG), with incubationat a temperature of 25, 26, 28, or 30° C. and a variant incubation timeranging from 11/2 h to up to 720 h.

Half-lives and HIFs were calculated as described above in Example 3. Incases where the difference in stability between wild-type and variantswas too big to accurately assess half-life for both wild-type andvariant using the same incubation time, the incubation time forwild-type and variant is different, e.g. 1 h for wild-type and up to 720h for the most stable variants.

Tables 8-12 below show the HIFs for the purified variants along withinformation on the test conditions (incubation temperature, detergentconcentration, incubation time) for each variant. HIFs of all variantswere calculated based on the wild-type endoglucanase (SEQ ID NO:2)incubated at the same detergent concentration and temperature (HIF ofwild-type=1 in all tables, per definition).

TABLE 8 Variants of the mature parent GH9 endoglucanase (SEQ ID NO: 2)with corresponding half-life improvement factors (HIF) measured at atemperature of 25° C. Alterations compared to PUG (detergent Incubationtime reference (SEQ ID NO: 2) concentration) (hrs) HIF A885F 90% PUG 161.1 S595L 90% PUG 16 1.1 T292A 90% PUG 18 1.1 F638I 90% PUG 16 1.5P570G + V837I 90% PUG 16 2.1

TABLE 9 Variants of the mature parent GH9 endoglucanase (SEQ ID NO: 2)with corresponding half-life improvement factors (HIF) measured at atemperature of 26° C. Alterations compared to PUG (detergent Incubationreference (SEQ ID NO: 2) concentration) time (hrs) HIF S593N 90% PUG 181.1 A957L 90% PUG 18 1.1 A942P 90% PUG 18 1.2 K281R 90% PUG 18.15 1.2T932A 90% PUG 18 1.4 V938I 90% PUG 18 1.4 N933S 90% PUG 18 1.5 A937E 90%PUG 18 1.6 Y884H 90% PUG 18 1.9 N941S 90% PUG 18 2.1 F638I 90% PUG 18.52.2 Y579W + K451Q 90% PUG 18 2.3 K939V 90% PUG 18 2.5 T711S + Y579W 90%PUG 19.5 2.6 Y579W + K451S + T972K 90% PUG 18 2.8 K713R + Y579W 90% PUG19.5 2.9 A564I 90% PUG 18 2.9

TABLE 10 Variants of the mature parent GH9 endoglucanase (SEQ ID NO: 2)with corresponding half-life improvement factors (HIF) measured at atemperature of 28° C. Alterations compared to PUG (detergent Incubationreference (SEQ ID NO: 2) concentration) time (hrs) HIF Y579W + Q834E 90%PUG 5.5 3.5 Y579W + E408D 90% PUG 5.5 3.9

TABLE 11 Variants of the mature parent GH9 endoglucanase (SEQ ID NO: 2)with corresponding half-life improvement factors (HIF) measured at atemperature of 30° C. Alterations compared to reference PUG (detergentIncubation (SEQ ID NO: 2) concentration) time (hrs) HIF A559N + Y579W +T639G 90% PUG 16 3.5 A559N + Y579W + T639I + R898Q 90% PUG 16 3.5K451S + K627R 90% PUG 16 3.8 Q4165 + F638I 90% PUG 16 3.9 V4T + A559N +Y579W 90% PUG 16 3.9 F638I + K921R 90% PUG 16 4.0 Q4165 + S636N 90% PUG16 4.1 S636N + K921R 90% PUG 16 4.1 Y579W + S636N 90% PUG 16 4.1 Y55M +K627R + Y1042N 90% PUG 16 4.5 A559N + Y579W + S636L 90% PUG 16 4.7H149X + A559N + K627R 90% PUG 16 5.2 A448W + A559N + Y579W 90% PUG 165.7 K627R + K921R 90% PUG 16 5.9 E53Y + N216R + K627R 90% PUG 16 6.1E53Y + K627R 90% PUG 16 6.3 K627R + F638I 90% PUG 16 6.5 A448E + Y934G90% PUG 16 6.7 A559N + Y579W + A651P + S835A 90% PUG 168 6.7 P570K +F638I 90% PUG 16 7.0 E53Y + N216R + Y934G 90% PUG 16 7.4 F20P + F638I90% PUG 16 7.4 K4515 + A559N + Y579W + S636N 90% PUG 168 8.5 F20P +A559N + Y579W 90% PUG 16 8.7 K4515 + A559N + Y579W + S636N 90% PUG 1689.0 E4085 + A559N + Y579W 90% PUG 16 9.6 Y579W + K627R 90% PUG 16 9.9P570T + Y934G 90% PUG 16 11.4 K512P + P570K 90% PUG 168 11.6 P570Q +K627R 90% PUG 16 11.6 A559N + Y579W + K627R 90% PUG 16 11.7 A559N +Y579W + I602T + V603P 90% PUG 168 13.2 P570T + E408D 90% PUG 16 14.3F20P + K627R 90% PUG 16 16.1 A559N + Y579W + I602T + V603P + S616D 90%PUG 168 17.3 A559N + Y579W + I602T + V603P + S616D + K921R 90% PUG 16818.0 E408D + A651P 90% PUG 16 18.4 P570Q + Y579W 90% PUG 16 19.0 Y55M +A559N + Y579W + A651P + Y934G 90% PUG 168 19.6 K51Q + K627R + Y934G 90%PUG 16 19.8 E408D + A559N + P570A + Y579W + K921R 90% PUG 16 20.7E408D + K512P + A559N + Y579W + F638I + A885F + K921R 90% PUG 16 20.7E408D + Q4165 + A559N + Y579W + S616D + K921R + Y934G 90% PUG 16 20.7F20N + K627R + S636N + A651P + K921R + Y934G 90% PUG 16 20.7 F20N +S616D + K627R + A651P + K921R + Y934G 90% PUG 16 20.7 F20N + S616D +K627R + S636N + A651P + Y934G 90% PUG 16 20.7 K627R + S636N + A651P +K921R + Y934G 90% PUG 16 20.7 P570K + K627R + F638I 90% PUG 16 20.7P570Q + E408D 90% PUG 16 20.7 P570R + K627R + S636N 90% PUG 16 20.7P570S + K627R + S636N 90% PUG 16 20.7 S616D + K627R + S636N + A651P +K921R + Y934G 90% PUG 16 20.7 A559N + S560P + Y579W + K627R 90% PUG 1621.3 E408D + A559N + Y579W + S636N + K921R + Y934G 90% PUG 16 21.7E408D + A559N + Y579W + S616D + K921R 90% PUG 16 22.2 K627R + A651P +Y934G 90% PUG 138 24.4 F20P + F638I 90% PUG 138 25.7 S616D + K627R +K921R + Y934G 90% PUG 138 25.7 F20N + E408D + A559N + Y579W + K921R +Y934G 90% PUG 16 25.7 E408D + Y934G 90% PUG 16 25.9 E408D + Q416D +A559N + Y579W + I602T + V603P + 90% PUG 16 26.0 S616D + K921R + Y934GF20P + E53Y + Q416D + A448E + K627R + Y934G 90% PUG 138 26.1 E408D +A559N + Y579W + S636N + K921R 90% PUG 16 26.1 V56M + K627R + Y934G 90%PUG 138 26.9 E53Y + Y55M + E408D + A559N + Y579W + S636N + 90% PUG 1627.8 K921R + Y934G K627R + F638I + Y934G 90% PUG 138 27.8 K51Q + K627R +Y934G 90% PUG 138 28.0 K451S + A559N + Y579W + I602T + V603P + S636N 90%PUG 168 28.1 S616D + K627R + A651P + K921R + Y934G + G994D 90% PUG 13828.4 F20N + S616D + K627R + S636N + Y934G 90% PUG 138 28.5 A448E +K627R + Y934G 90% PUG 138 28.5 E408D + K451S + A651P 90% PUG 16 28.6K627R + S636N + Y934G 90% PUG 138 28.7 K627R + K921R + Y934G 90% PUG 13828.9 S6070 + S1038G 90% PUG 138 29.0 K627R + S636K + Y934G 90% PUG 13830.0 F20P + Q416D + A559N + Y579W + K627R 90% PUG 138 30.7 A559N +Y579W + K627R + Y934G 90% PUG 138 30.9 F20N + S616D + K627R + Y934G 90%PUG 138 30.9 F20N + E408D + A559N + Y579W + S616D + K921R + Y934G 90%PUG 16 31.3 P570Q + Y579W 90% PUG 138 31.5 K627R + Q834E + Y934G 90% PUG138 31.6 Q416S + A448W + A559N + K627R + Y934G 90% PUG 138 31.9 P570K +K627R + Y934G 90% PUG 138 32.3 A559N + Y579W + S616D + K627R 90% PUG 13832.9 F20N + S616D + K627R + A651P + K921R + Y934G 90% PUG 138 33.4F20N + E408D + A559N + Y579W + K627R + K921R + Y934G 90% PUG 16 33.8K512P + K627R + Y934G 90% PUG 138 33.8 S616D + K627R + A651P + K921R +Y934G 90% PUG 138 34.6 S616D + K627R + S636N + A651P + K921R + Y934G 90%PUG 138 34.9 F20P + A448E + K627R + Y934G 90% PUG 138 35.5 K627R +S636N + A651P + K921R + Y934G 90% PUG 138 36.1 F20N + E408D + Q416S +A559N + Y579W + S616D + 90% PUG 16 36.1 K921R + Y934G P570K + K627R +F638I 90% PUG 138 36.1 S616D + K627R + S636N + K921R + Y934G 90% PUG 13836.5 F20N + K627R + Y934G 90% PUG 138 36.9 P570T + K627R + Y934G 90% PUG138 36.9 F20P + E53Y + Q416D + K627R + Y934G 90% PUG 138 37.1 F20P +E53Y + K627R + Y934G 90% PUG 138 37.3 P570Q + K627R + Y934G 90% PUG 13838.1 P570K + Y579W + Y934Q 90% PUG 138 38.1 F20N + S616D + K627R +S636N + A651P + Y934G 90% PUG 138 39.4 P570S + K627R + S636N 90% PUG 13839.6 A559N + Y579W + S616D + K627R + S636N 90% PUG 138 40.1 S616D +K627R + S636N + A651P + Y934G 90% PUG 138 41.5 Q416D + P570K + Y579W 90%PUG 138 41.9 A559N + Y579W + S616D + K627R + A651P 90% PUG 138 42.2A559N + Y579W + S616D + K627R + A651P 90% PUG 168 42.2 P570K + Y579W +A651P 90% PUG 138 43.0 P570Q + K627R + A651P 90% PUG 138 43.0 P570R +K627R + S636N 90% PUG 138 43.5 F20N + S616D + K627R + K921R + Y934G 90%PUG 138 43.7 E408N + Q416D + K627R + S636N 90% PUG 138 44.4 F20P +A559N + Y579W + K627R 90% PUG 138 44.4 A559N + Y579W + S616D + K627R +S636N + A651P 90% PUG 168 44.5 P570K + Y579W + Y934G 90% PUG 138 44.5P570Q + K627R + Y934R 90% PUG 138 44.5 Q416S + P570K + Y579W 90% PUG 16844.7 A559N + Y579W + S616D + K627R + S636N + A651P 90% PUG 138 44.9F20N + A559N + Y579W + S616D + K627R + A651P 90% PUG 138 44.9 F20P +E53Y + A559N + Y579W + K627R 90% PUG 138 46.2 K627R + S636N + Q416D +E408N + Q416S 90% PUG 138 46.2 P570K + Y579W + K927R 90% PUG 138 46.6E408D + S636N + A651P + N441G 90% PUG 168 46.7 F20P + Q416D + K627R +Y934G 90% PUG 138 47.9 P570K + Y579W + Q416D 90% PUG 138 48.8 A559N +S560P + Y579W + K627R 90% PUG 138 49.9 F20N + K627R + S636N + K921R +Y934G 90% PUG 138 50.1 E408D + A559N + Y579W + I602T + V603P + S616D +K921R 90% PUG 16 50.1 F20N + A559N + Y579W + S616D + K627R + S636N +A651P 90% PUG 138 52.1 K512P + P570K + Y579W 90% PUG 138 52.3 F20N +K627R + S636N + A651P + K921R + Y934G 90% PUG 138 52.4 E408D + S636N +A651P + 1575V 90% PUG 168 52.7 F20N + S616D + K627R + S636N + S642N +K921R + Y934G 90% PUG 138 52.9 P570K + Y579W + S636N 90% PUG 138 53.6E408D + A559N + Y579W + K921R 90% PUG 16 54.1 A559N + P570S + Y579W +K627R 90% PUG 138 55.3 A559N + S560P + Y579W + K627R 90% PUG 138 55.5E408S + A559N + Y579W + K627R 90% PUG 138 55.7 P570K + Y579W + K627M 90%PUG 138 55.9 E408D + S636N + A651P + A924D 90% PUG 168 56.5 E408D +Y579W 90% PUG 16 56.5 E408N + Q416D + Y579W + K627R 90% PUG 138 57.3A559N + P570T + Y579W + K627R 90% PUG 138 58.0 E408D + S636N + A651P +A688G 90% PUG 168 58.1 P570Q + E408D 90% PUG 138 58.4 E408D + S636N +A651P + V756H 90% PUG 168 58.5 E408N + K627R + S636N 90% PUG 138 58.8E408D + Y579W + A937E + F20P + T887S + S636K 90% PUG 168 59.9 E408D +S636N + A651P + T883R + Q956Y 90% PUG 168 60.4 E408D + S636N + A651P +N216D 90% PUG 168 60.5 E408D + A559N + Y579W + S616D + K921R + Y934R +A937E + K939V 90% PUG 138 60.7 F20N + E408D + Q416S + A559N + Y579W +S616D + K921R 90% PUG 138 60.9 E408D + Q416S + A559N + Y579W + S616D +K921R + Y934G 90% PUG 138 61.2 A559N + P570R + Y579W + K627R 90% PUG 13861.8 E408D + Y934G 90% PUG 138 61.8 F20P + E408D + Y579W + S636K + A937E90% PUG 168 62.1 E408D + S636N + A651P + K720H 90% PUG 168 62.1 F20N +E408D + A559N + Y579W + K921R + Y934G + T883R 90% PUG 168 63.2 E408D +A559N + Y579W + S616D + K921R + Q298E 90% PUG 138 63.5 E408D + A559N +Y579W + S616D + K921R + Y934R + A937E + K939V 90% PUG 138 63.7 E408D +Y579W + A651P + I602T + Q416S + S560P 90% PUG 168 63.8 E408D + Y579W +A937E + F20P + S636K 90% PUG 168 63.8 E408D + A559N + Y579W + I602T +V603P + S616D + 90% PUG 138 64.9 K921R + Y934G + A885Q E408D + A559N +Y579W + K921R 90% PUG 168 65.1 K627R + S636N + Q416D + E408N + Y579W 90%PUG 138 66.2 E408D + K512P + A559N + Y579W + F638I + A885F + K921R 90%PUG 138 66.3 E408D + Y579W + A651P + I602T + Q416S + V926P 90% PUG 16866.5 E408D + A559N + Y579W + S616D + K921R + Y934G + 90% PUG 138 66.6A937E + K939V E408D + A559N + P570A + Y579W + K921R 90% PUG 138 67.0E408D + A559N + Y579W + S616D + K921R + D635A 90% PUG 138 67.6 E408D +A559N + Y579W + S616D + K921R 90% PUG 138 67.7 S17A + F20P + Q416D +P570K + Y579W 90% PUG 138 68.0 E408D + Y579W + A937E + F20P + S560P 90%PUG 138 69.1 E408N + Q416D + P570K + Y579W 90% PUG 138 69.5 F20N +E408D + Q416S + A559N + Y579W + S616D + 90% PUG 138 70.3 K921R + Y934GA559N + Y579W + P570K + Y934R + Q416S 90% PUG 168 70.3 P570K + Y579W +Q416D + E408N + S17A + F20P 90% PUG 138 70.5 E408D + A559N + Y579W +I602T + V603P + S616D + 90% PUG 168 71.6 K921R + Y934G + Y884H E408D +A559N + Y579W + S616D + K921R + Y934G 90% PUG 138 71.9 A559N + P570Q +Y579W + K627R 90% PUG 138 72.1 E408D + Q416D + A559N + Y579W + K921R +Y934G 90% PUG 138 72.1 F20N + P570K + Y579W 90% PUG 138 72.9 A559N +Y579W + P570K + Y934R + Q416D 90% PUG 168 72.9 A559N + Y579W + P570K +Y934R + F20P 90% PUG 168 74.9 E408D + Y579W + A651P + I602T + Q416S +V756Y 90% PUG 168 75.4 E408D + A559N + Y579W + I602T + V603P + S616D +90% PUG 138 75.9 K921R + Y934G + A885F E408D + Y579W + A651P + I602T +Q416S + I403Y 90% PUG 168 76.1 E408D + A559N + Y579W + I602T + V603P +S616D + 90% PUG 138 76.2 K921R + Y934G + Q834E E408D + A559N + Y579W +S616D + K921R + S313D 90% PUG 138 76.2 E408D + K451S + A651P 90% PUG 13876.2 E408D + S636N + A651P + H182Y 90% PUG 138 76.6 E408D + Y579W +A937E + F20P + V603P 90% PUG 138 77.8 E408D + A559N + Y579W + K921R 90%PUG 138 77.9 Y579W + A937E 90% PUG 138 77.9 E408D + Q416D + A559N +Y579W + K627R + K921R + GY934 90% PUG 138 78.0 E408D + S636N + A651P +K631R 90% PUG 138 78.2 A559N + Y579W + P570K + Y934R + K627R 90% PUG 16878.7 P570K + Y579W + Q416D + S17A + F20P + E408N + Q416D 90% PUG 13879.1 E53Y + Y55M + E408D + A559N + Y579W + S636N + 90% PUG 138 80.1K921R + Y934G E408D + A559N + Y579W + K921R + Y934G + T697G + F20N 90%PUG 138 80.1 E408D + Y579W + A651P + I602T + Q416S + V881Q 90% PUG 16880.1 E408D + S636N + A651P + T999R 90% PUG 138 81.0 E408D + A651P 90%PUG 138 81.1 E408D + A559N + Y579W + S636N + K921R 90% PUG 138 81.6E408D + Q416D + A559N + P570R + Y579W + K921R + Y934G 90% PUG 138 82.3E408D + Y579W + Q834E 90% PUG 138 82.5 F20N + E408D + A559N + Y579W +K921R + Y934G 90% PUG 138 82.7 A559N + Y579W + P570K + Y934R + A651P 90%PUG 138 83.2 E408D + S636N + A651P + F1048W 90% PUG 138 83.3 E408D +Y579W + A651P + I602T + Q416S + Y579W 90% PUG 168 83.9 E408D + A559N +P570Q + Y579W + K921R + Y934G 90% PUG 138 84.1 E408D + S636N + A651P +R880K 90% PUG 138 84.1 F20P + E408D + Y579W + A937E 90% PUG 138 84.2E408D + A559N + Y579W + I602T + V603P + S616D + 90% PUG 138 84.5 K921R +A1037E E408D + A559N + Y579W + S616D + K921R + T697G + A885Q 90% PUG 13884.5 E408D + Y579W + A937E + F20P + T883R 90% PUG 138 84.7 E408D +Y579W + A651P + I602T + Q416S + K627R 90% PUG 168 85.1 F20N + E408D +A559N + Y579W + K921R + Y934G + T999R 90% PUG 138 85.3 E408D + Y579W +A937E + F20P + Q834E 90% PUG 138 85.5 P570K + Y579W + Q416D + S17A +F20P + Y934R 90% PUG 138 87.1 E408D + S636N + A651P 90% PUG 138 87.6P570K + Y579W + Q416D + S17A + F20P + I602T 90% PUG 138 87.6 E408D +S636N + A651P + Q298E 90% PUG 138 88.0 E408D + Y579W + A651P + I602T +Q416S + A559N 90% PUG 168 88.1 E408D + S636N + A651P + V926P 90% PUG 13888.9 E408D + A559N + Y579W + I602T + V603P + S616D + 90% PUG 138 88.9K921R + Y934G + S636K E408D + A559N + Y579W + S636N + A651P + K921R +Y934G 90% PUG 138 89.0 E408D + A559N + Y579W + S616D + K921R + V756Y 90%PUG 138 89.5 P570K + Y579W + Q416D + S17A + F20P + I602T + V603P 90% PUG138 89.5 E408D + A559N + Y579W + S636N + K921R + Y934G 90% PUG 138 90.6E408D + Y579W + A937E + F20P + S636K 90% PUG 138 90.8 E408D + Q416D +A559N + Y579W + I602T + V603P + 90% PUG 1 38 90.9 S616D + K921R +Y934G + A651P + A885F E408D + A559N + Y579W + I602T + V603P + S616D +90% PUG 138 91.1 K921R + Y934G + S17A E408D + A559N + Y579W + I602T +V603P + S616D + 90% PUG 138 91.5 K921R + Y934G E408D + A559N + Y579W +S616D + T697G + V756Y + K921R 90% PUG 138 91.5 E408D + Y579W + A651P +I602T + Q416S + S17A + F20P 90% PUG 168 93.5 F20N + E408D + A559N +Y579W + K627R + K921R + Y934G 90% PUG 138 93.9 A559N + Y579W + P570K +Y934R + E408D 90% PUG 168 94.3 E408D + Q416D + A559N + Y579W + K921R 90%PUG 168 94.7 E408D + A559N + Y579W + K921R + Y934G + A937E + K939V 90%PUG 138 95.0 E408D + S636N + A651P + V756H + T883R 90% PUG 168 95.0E408D + Y579W + A937E + F20P + S17A + F20P 90% PUG 138 95.0 E408D +A559N + Y579W + I602T + V603P + S616D + K921R 90% PUG 138 95.5 F20N +E408D + A559N + Y579W + K921R + Y934G + I302D 90% PUG 138 95.9 E408D +Q416S + Y579W + I602T + A651P + R880K 90% PUG 138 96.7 F20P + E408D +Q416S + A559N + S560P + P570R + 90% PUG 168 97.7 Y579W + K627R + K921R +Y934R F20P + E408D + A559N + Y579W + K921R + Y934G 90% PUG 138 98.0E408D + A559N + Y579W + I602T + V603P + S616D + 90% PUG 168 98.1 K921R +Y934G + A937E + K939V E408D + S636N + A651P + T883K 90% PUG 138 98.5N18G + A71E + L226K + E408D + Y579W + I602T + A651P + 90% PUG 168 98.5A688G + V756Y + T887S E408D + A559N + Y579W + I602T + V603P + S616D +90% PUG 138 101.3 K921R + Y934R + A937E + K939V E408D + A559N + Y579W +I602T + V603P + S616D + K921R 90% PUG 138 102.0 E408D + A559N + Y579W +S616D + K921R + T697G + A65I 90% PUG 138 102.2 F20N + E408D + A559N +Y579W + K921R + Y934G + R880K 90% PUG 138 102.2 A559N + Y579W + P570K +Y934R + E408N 90% PUG 138 102.9 E408D + Y579W + A651P 90% PUG 138 103.7P570K + Y579W + Q416D + S17A + F20P + A651P 90% PUG 138 103.7 F20P +E408D + Q416D + A559N + S560P + P570R + 90% PUG 168 103.8 Y579W +K627R + S636N + F638I + K921R + Y934R E408D + A559N + Y579W + S616D +T697G + K921R 90% PUG 138 104.1 F20P + E408D + Y579W + S636K + T697G +A937E 90% PUG 168 105.4 E408D + A559N + Y579W + I602T + V603P + S616D +90% PUG 138 106.4 K921R + N933S + Y934R + A937E + V9381 + K939V +N941S + A942P F20P + E408D + Y579W + A651P 90% PUG 138 106.9 K51Q +E408D + Y579W + A651P 90% PUG 138 109.7 A559N + Y579W + P570K + Y934R +Q683E 90% PUG 138 109.9 F20P + E408D + Q416D + A559N + S560P + P570Q +90% PUG 168 109.9 Y579W + K627R + K921R + Y934G E408D + Q416D + A559N +Y579W + I602T + V603P + 90% PUG 168 110.9 S616D + A651P + K921R + Y934GE408D + A559N + Y579W + S616D + K921R + T697G + V881T 90% PUG 138 111.8E408D + A559N + Y579W + S636N + Q683E + K921R + Y934G 90% PUG 138 112.7E408D + S636N + A651P + V756Y 90% PUG 138 113.4 F20P + E408D + Y579W +S636K + A937E 90% PUG 168 113.9 E408D + S636N + A651P + A885Q 90% PUG138 114.4 E408D + S636N + A651P + V756H + T883K 90% PUG 168 118.9 F20P +E408D + Q416S + A559N + S560P + P570R + 90% PUG 168 121.5 Y579W +K627R + S636N + F638I + K921R + Y934G E408D + Y579W + I602T + A651P 90%PUG 138 122.1 E408D + S636N + A651P + T697G + T883R 90% PUG 168 123.5E408D + S636N + A651P + V756H + R880K 90% PUG 168 124.3 E408D + Y579W +A651P + I602T + R880K 90% PUG 168 124.5 F20N + E408D + A559N + Y579W +K921R + Y934G + 90% PUG 138 125.1 T697G + Y55M E408D + Q416D + A559N +Y579W + I602T + V603P + 90% PUG 138 125.4 S616D + K921R + Y934G S17A +F20P + E408D + Y579W + I602T + A651P + 90% PUG 168 127.3 F906A + Y934GF20N + E408D + A559N + Y579W + K921R + Y934G + T8875 90% PUG 138 127.7E408D + S636N + A651P + V756H + Y579W 90% PUG 168 129.7 E408D + S636N +A651P + S313D 90% PUG 138 132.5 E408D + Y579W + A651P + I602T + V881T90% PUG 138 135.9 E408D + Y579W + A651P + I602T + T887S 90% PUG 138138.8 E408D + Y579W + I602T + F638N + A651P + R880K + 90% PUG 168 139.5K921R + Y934G E408D + S636N + A651P + T697G 90% PUG 138 140.2 E408D +S636N + A651P + V756H + I602T + V603P 90% PUG 168 140.7 E408D + A559N +Y579W + S616D + K921R + T697G + 90% PUG 138 141.8 I602T + V603P E408D +Q416D + A559N + Y579W + I602T + V603P + 90% PUG 138 142.7 S616D +K921R + Y934G + A651P + Y884H F20N + E408D + A559N + Y579W + K921R +Y934G + V756Y 90% PUG 138 143.9 E408D + S636N + A651P + V756H + A885Q90% PUG 168 144.7 S17A + F20P + E408D + Y579W + I602T + A651P + F906A +90% PUG 168 150.2 Y934G + S636K F20N + E408D + A559N + Y579W + K921R +Y934G + 90% PUG 138 150.6 T697G + T887K F20N + E408D + A559N + Y579W +K921R + Y934G + 90% PUG 138 151.1 T697G + A885Q E408D + Y579W + A651P +I602T + R880K 90% PUG 138 151.2 S17A + F20P + E408D + Y579W + I602T +A651P + 90% PUG 168 151.5 F906A + Y934G + V881Q I302D + E408D + Q4165 +Y579W + I602T + A651P + 90% PUG 168 152.0 R880K + Y934Q S17A + F20P +E408D + Y579W + I602T + A651P + 90% PUG 168 153.5 F906A + Y934G + L888MS17A + F20P + E408D + Y579W + I602T + A651P + 90% PUG 168 154.1 F906A +Y934G + S560P I302D + E408D + Q4165 + Y579W + I602T + A651P + 90% PUG168 154.9 R880K + K744Q + N848D + A868E F20P + S313D + E408D + Y579W +S636K + T697G + 90% PUG 168 155.5 T887K + A937E F20P + S313D + E408D +Y579W + S636K + T697G + 90% PUG 168 156.2 A937E + A885Q E408D + A559N +Y579W + S616D + K921R + T697G + Y934G 90% PUG 138 156.9 S17A + F20P +E408D + Y579W + I602T + A651P + 90% PUG 168 157.2 F906A + Y934G + T887KE408D + Y579W + A651P + I602T + V881Q 90% PUG 138 157.6 E408D + S636N +A651P + A688G + A283D 90% PUG 168 162.3 S17A + F20P + E408D + Y579W +I602T + A651P + 90% PUG 168 163.7 N905D + T825G A559N + Y579W + A688G +V756Y + K921R + Y934G 90% PUG 168 164.8 E408D + S636N + A651P + V756H +Y934Q 90% PUG 168 165.3 S17A + F20P + E408D + Y579W + I602T + A651P +F906A 90% PUG 168 166.2 E408D + Q4165 + Y579W + I602T + A651P + N848D +A868E 90% PUG 168 168.9 E408D + S636N + A651P + A688G + D476R 90% PUG168 169.4 I302D + E408D + Q4165 + Y579W + I602T + A651P + 90% PUG 168171.5 R880K + V756H S17A + F20P + E408D + Y579W + I602T + A651P +F906A + 90% PUG 168 172.3 Y934G + S636N E408D + Q416D + A559N + Y579W +I602T + V603P + 90% PUG 138 176.9 S616D + K921R + Y934G + A651P + T8875S17A + F20P + E408D + Y579W + I602T + A651P + N848D 90% PUG 168 178.3E408D + Q416S + Y579W + I602T + A651P + V756Y 90% PUG 168 181.5 E408D +Q4165 + Y579W + I602T + A651P + T697G 90% PUG 168 204.0

TABLE 12 Variants of the mature parent GH9 endoglucanase (SEQ ID NO: 2)with corresponding half-life improvement factors (HIF) measured at atemperature of 30° C. PUG (detergent Incubation Alterations compared toreference (SEQ ID NO: 2) concentration) time (hrs) HIF E408D + A559N +Y579W + K921R + Y934R + A937E + K939V 95% PUG 168  72.0 E408D + A559N +Y579W + K921R + Y934R + A937E + 95% PUG 168  74.7 V9381 + K939V +N9415 + A942P E408D + Y579W + A651P + I602T + Q416S 95% PUG 168  80.0E408D + Y579W + A937E + F20P + Q683E 95% PUG 168  82.7 E408D + S636N +A651P + A283D 95% PUG 336  91.3 E408D + S636N + A651P + N18G 95% PUG 336 91.3 E408D + Y579W + A651P + I602T + S17A + F20P 95% PUG 168  91.3E408D + S636N + A651P + A346D 95% PUG 336  96.7 E408D + S636N + A651P +A71E + A120P + A186P + D247N 95% PUG 168 102.4 F20N + E408D + A559N +Y579W + Q683E + K921R + Y934G 95% PUG 168 112.7 E408D + Y579W + A651P +I602T + Y934Q 95% PUG 168 113.3 E408D + S636N + A651P + T872G 95% PUG336 116.7 E408D + Y579W + A651P + I602T + T883R 95% PUG 168 120.0 F20N +E408D + A559N + Y579W + T697G + K921R + Y934G 95% PUG 168 122.0 E408D +Y579W + A651P + I602T + Q4165 + R880K + N216D 95% PUG 336 125.3 E408D +A559N + Y579W + I602T + V603P + S616D + 95% PUG 168 129.5 K921R +Y934G + Q683E E408D + Y579W + A651P + I602T + Q4165 + R880K + F20P 95%PUG 336 139.2 E408D + S636N + A651P + N848D 95% PUG 336 141.7 E408D +Y579W + A651P + I602T + Q4165 + R880K + I302D 95% PUG 336 148.8 E408D +Y579W + I602T + F638N + A651P + R880K + K921R + 95% PUG 672 152.9Y934G + K51Q F20N + E408D + A559N + Y579W + K921R + Y934G + 95% PUG 168156.0 T697G + K512P E408D + Y579W + A651P + I602T + Q4165 + R880K +Y884H 95% PUG 336 156.9 N18G + A120P + E408D + S636N + A651P + A688G +V756Y 95% PUG 672 161.5 E408D + S636N + A651P + V756H + S313D 95% PUG750 161.7 N18G + A71E + A186P + E408D + Y579W + I602T + A651P + 95% PUG672 161.7 A688G + V756Y E408D + S636N + A651P + V756H + T697G 95% PUG672 164.9 E408D + Y579W + A651P + I602T + T883R + V756Y 95% PUG 750167.6 F20P + E408D + Y579W + 5636K + T697G + A937E + V926F 95% PUG 336168.4 E408D + A559N + Y579W + S616D + K921R + T697G + I602T 95% PUG 672168.9 N216D + I302D + E408D + Q4165 + Y579W + I602T + A651P + 95% PUG672 170.3 A688G + R880K + V890R + N905D E408D + Y579W + I602T + F638N +A651P + N848D + 95% PUG 168 172.6 R880K + K921R + Y934G N18G + N189K +E408D + A559N + Y579W + A688G + 95% PUG 750 175.7 T697G + V756Y +K921R + Y934G E408D + Y579W + I602T + F638N + A651P + V756Y + R880K +95% PUG 672 181.0 K921R + Y934G + P570R S313D + E408D + Y579W + I602T +F638N + A651P + R880K + 95% PUG 750 182.5 K921R + Y934G E408D + Y579W +A651P + I602T + T883R + N848D 95% PUG 750 183.1 E408D + Y579W + I602T +F638N + A651P + R880K + 95% PUG 672 183.3 N905D + K921R + Y934G + A283DE408D + Y579W + I602T + F638N + A651P + R880K + 95% PUG 672 183.3N905D + K921R + Y934G + A885Q E408D + Y579W + I602T + F638N + A651P +R880K + 95% PUG 750 183.4 N905D + K921R + Y934G S17A + F20P + E408D +Y579W + I602T + A651P + F906A + 95% PUG 750 183.7 Y934G + S313D E408D +Y579W + I602T + F638N + A651P + R880K + K921R + 95% PUG 672 183.8Y934G + Y55D E408D + Y579W + I602T + F638N + A651P + R880K + K921R + 95%PUG 672 184.0 Y934G + N848D S313D + I302D + E408D + Y579W + I602T +F638N + A651P + 95% PUG 672 186.3 R880K + K921R + Y934G N18G + A186P +E408D + Q416S + Y579W + I602T + A651P + 95% PUG 672 186.8 A688G + V756YE408D + Y579W + A651P + I602T + T883R + A688G 95% PUG 750 188.3 E408D +Y579W + I602T + A651P + T697G + T883R 95% PUG 672 189.9 N18G + E408D +S636N + A651P + A688G + T697G + V756Y 95% PUG 750 190.9 N18G + E408D +A559N + Y579W + I602T + V603P + S616D + 95% PUG 672 196.2 T697G +V756Y + K921R + T999R F20N + A346D + E408D + K512P + A559N + Y579W +I602T + 95% PUG 672 197.8 S636N + A651P + T697G + V756Y + A824D +A885N + K921R + Y934G F20N + E408D + Q489P + K512P + A559N + Y579W +I602T + 95% PUG 672 198.0 A651P + T697G + V756Y + A885N + K921R +Y934G + A937E E408D + Y579W + I602T + F638N + A651P + R880K + K921R +95% PUG 672 200.7 Y934G + V756Y E408D + Y579W + I602T + F638N + A651P +R880K + 95% PUG 672 200.8 N905D + K921R + Y934G + N848D S17A + F20P +E408D + Y579W + I602T + A651P + A688G 95% PUG 672 202.0 E408D + S636N +A651P + A688G + N848D + V881Q + N905D 95% PUG 168 202.3 F20N + E408D +K512P + A559N + Y579W + T697G + K921R + 95% PUG 336 202.3 Y934G + A651PF20P + Y55M + S313D + E408D + Y579W + S636K + T697G + 95% PUG 672 202.8A937E I302D + E408D + Q4165 + Y579W + I602T + A651P + R880K + 95% PUG672 204.5 T697G N18G + A186P + E408D + Y579W + I602T + A651P + A688G +95% PUG 750 204.5 V756Y + V881T S17A + F20P + E408D + Y579W + I602T +A651P + F906A + 95% PUG 672 205.5 Y934G + A688G I302D + S313D + E408D +Q4165 + Y579W + I602T + A651P + 95% PUG 672 206.3 A688G + R880K +T892V + N905D + Q912V E408D + S636N + A651P + T697G + S313D 95% PUG 672206.6 F20P + S313D + E408D + Y579W + S636K + T697G + A937E + 95% PUG 672207.3 V603P F20P + S313D + E408D + Y579W + S636K + T697G + A937E + 95%PUG 672 207.5 T887S E408D + Y579W + I602T + F638N + A651P + R880K +N905D + 95% PUG 672 207.9 K921R + Y934G + A885N I302D + E408D + Q4165 +Y579W + I602T + A651P + A688G + 95% PUG 750 212.4 R880K E408D + Y579W +I602T + F638N + A651P + V756Y + R880K + 95% PUG 168 214.2 K921R + Y934GE408D + Y579W + I602T + F638N + A651P + A688G + 95% PUG 672 214.9K720H + R880K + K921R + Y934G I302D + E408D + Q4165 + Y579W + I602T +A651P + A688G + 95% PUG 672 215.5 W719R + R880K + N905D + F1048W K51Q +E408D + Y579W + I602T + F638N + A651P + R880K + 95% PUG 672 216.0K921R + Y934G I302D + E408D + Q4165 + Y579W + I602T + A651P + A688G +95% PUG 672 222.1 W719R + R880K + N905D + F1048W I302D + E408D + Q416S +Y579W + I602T + A651P + A688G + 95% PUG 672 224.2 V881Q + K921R Y55M +A283D + S313D + E408D + Y579W + I602T + F638N + 95% PUG 672 224.3A651P + K720H + V756Y + N848D + A869V + R880K + Q912V + K921R + Y934RF20P + S313D + E408D + V567P + Y579W + S636K + A651P + 95% PUG 672 224.6T697G + N848D + T883R + A937E + Q956Y + F1048W F20P + S313D + E408D +Y579W + S636K + T697G + A937E + 95% PUG 672 226.1 T883C S313D + A346D +E408D + Y579W + I602T + F638N + A651P + 95% PUG 672 230.5 T697G +N848D + R880K + A885N + K921R + Y934G E408D + Y579W + I602T + F638N +A651P + R880K + 95% PUG 672 231.7 N905D + K921R + Y934G + A688G E408D +Y579W + I602T + F638N + A651P + V756Y + R880K + 95%PUG 672 232.3 K921R +Y934G + N848D I302D + E408D + Q416S + Y579W + I602T + A651P + A688G +95% PUG 672 232.9 V881Q + K921R F20P + S313D + E408D + Y579W + S636K +T697G + A937E + 95% PUG 672 235.3 A885N F20P + S313D + E408D + Y579W +S636K + T697G + A937E + 95% PUG 750 236.9 E53Y F20P + S313D + E408D +Y579W + S636K + T697G + A937E + 95% PUG 750 238.2 R880K S17A + F20P +E408D + Y579W + I602T + A651P + F906A + 95% PUG 750 240.0 Y934G + T697GY55M + A283D + S313D + E408D + Y579W + I602T + F638N + 95% PUG 672 243.1A651P + K720H + V756Y + N848D + A869V + R880K + N905D + K921R + Y934RS313D + E408D + S560P + Y579W + I602T + F638N + A651P + 95% PUG 672244.9 W719R + V756Y + T825G + N848D + A869V + D870V + R880K + N905D +K921R + Y934G F20P + S313D + E408D + Y579W + S636K + T697G + A937E + 95%PUG 750 247.1 T883V S313D + E408D + S560P + Y579W + I602T + F638N +A651P + 95% PUG 672 250.9 W719R + V756Y + N848D + A869V + D870V +R880K + K921R + Y934G I302D + E408D + Q4165 + Y579W + I602T + A651P +A688G + 95% PUG 672 253.3 R880K + N905D F20P + S313D + E408D + Y579W +S636K + T697G + A937E 95% PUG 336 253.9 E408D + Y579W + I602T + F638N +A651P + V756Y + R880K + 95%PUG 672 254.7 K921R + Y934G + S313D E408D +Y579W + I602T + A651P + A688G + N848D + T883R 95% PUG 672 255.1 S17A +F20P + N216D + I302D + E408D + Q4165 + Y579W + 95% PUG 672 257.1 I602T +A651P + W719R + T883R + F906A + Y934G + Q956Y I302D + E408D + Q4165 +Y579W + I602T + A651P + A688G + 95% PUG 672 257.7 R880K + N905D + K921RS313D + E408D + Y579W + I602T + F638N + A651P + T697G + 95% PUG 672258.5 R880K + K921R + Y934G I302D + E408D + Q4165 + Y579W + I602T +A651P + A688G + 95% PUG 672 259.4 V881Q + N905D + K921R I302D + E408D +Q4165 + Y579W + I602T + A651P + A688G + 95% PUG 672 263.5 V881Q +N905D + K921R E408D + Y579W + I602T + A651P + A688G + N848D + 95% PUG672 268.3 T883R + M980I S313D + E408D + Y579W + I602T + F638N + A651P +T697G + 95% PUG 672 272.3 R880 K + K921R + Y934G S313D + E408D + S560P +Y579W + I602T + F638N + A651P + 95% PUG 672 272.7 W719R + V756Y +N848D + A869V + D870V + R880 K + N905D + K921R + Y934G N216Q + I302D +H311N + E408D + Q416S + Y579W + I602T + 95% PUG 672 275.1 A651P +A688G + T697G + W719R + R880K + N905D + Q912V F20P + S313D + E408D +Y579W + S636K + T697G + N848D + 95% PUG 750 285.8 T887K + A937E S313D +E408D + Y579W + I602T + F638N + A651P + T697G + 95% PUG 672 288.9R880K + K921R + Y934G Y55M + A283D + S313D + E408D + Y579W + I602T +F638N + 95% PUG 672 289.4 A651P + W719R + V756Y + N848D + A869V +R880K + K921R + Y934R F20N + E408D + K512P + A559N + Y579W + I602T +A651P + 95% PUG 672 290.1 T697G + V756Y + K921R + Y934G Y55M + A283D +S313D + E408D + Y579W + I602T + F638N + 95% PUG 672 291.9 A651P +W719R + V756Y + N848D + A869V + R880K + K921R + Y934R A283D + S313D +E408D + A559P + Y579W + I602T + F638N + 95% PUG 672 292.7 A651P +W719R + V756Y + N848D + A869V + D870V + R880K + N905D + K921R + Y934GF20P + I302D + S313D + E408D + D476R + Y579W + S636K + 95% PUG 672 293.3T697G + V756Y + V881Q + T887K + F906A + A937E S313D + E408D + S560P +Y579W + I602T + F638N + A651P + 95% PUG 672 295.1 W719R + V756Y +N848D + A869V + D870V + R880K + N905D + K921R + Y934G F20P + I302D +S313D + E408D + D476R + Y579W + S636K + 95% PUG 672 299.4 T697G +V756Y + T887K + F906A + A937E S17A + F20P + N216D + A283D + H311N +E408D + Y579W + 95% PUG 672 300.1 I602T + A651P + A688G + T883R +F906A + Y934G + Q956Y Y55M + A283D + S313D + E408D + A559P + Y579W +I602T + 95% PUG 672 303.3 F638N + A651P + T697G + W719R + N848D +R880K + K921R + Y934G + A937E F20P + S313D + E408D + Y579W + S636K +T697G + N848D + 95% PUG 750 304.5 T887K + A937E + G1041R F20P + S313D +E408D + V567P + Y579W + S636K + T697G + 95% PUG 672 307.5 N848D +A937E + Q956Y F20P + I302D + S313D + E408D + D476R + Y579W + S636K + 95%PUG 672 309.7 T697G + V756Y + V881Q + T887K + F906A + A937E I302D +E408D + Q416S + Y579W + I602T + A651P + A688G + 95% PUG 672 317.0W719R + R880K + N905D + F1048W S17A + F20P + N216D + A283D + H311N +E408D + S560P + 95% PUG 672 319.1 Y579W + I602T + A651P + A688G +T883R + F906A + Q912V + Y934G + Q956Y Y55M + A283D + S313D + E408D +Y579W + I602T + F638N + 95% PUG 672 321.6 A651P + W719R + V756Y +N848D + A869V + R880K + N905D + K921R + Y934R F20P + S313D + E408D +Y579W + S636K + T697G + N848D + 95% PUG 672 325.9 A937E + Q956Y F20N +Y55M + H311N + E408D + K512P + A559N + Y579W + 95% PUG 672 338.4 A651P +T697G + N848D + K921R + Y934G Y55M + A283D + S313D + E408D + Y579W +I602T + F638N + 95% PUG 672 338.5 A651P + W719R + V756Y + N848D +A869V + R880K + K921R + Y934R Y55M + A283D + I302D + S313D + E408D +Y579W + I602T + 95% PUG 672 342.9 F638N + A651P + W719R + V756Y +N848D + A869V + R880K + K921R + Y934R Y55M + A283D + S313D + E408D +Y579W + I602T + F638N + 95% PUG 672 345.3 A651P + W719R + V756Y +N848D + A869V + R880K + A885N + K921R + Y934R Y55M + A283D + S313D +E408D + Y579W + I602T + F638N + 95% PUG 672 345.9 A651P + W719R +V756Y + N848D + A869V + R880K + N905D + K921R + Y934R Y55M + A283D +I302D + S313D + E408D + Y579W + I602T + 95% PUG 672 348.9 F638N +A651P + W719R + V756Y + N848D + A869V + R880K + K921R + Y934R S313D +E408D + Y579W + I602T + F638N + A651P + T697G + 95% PUG 672 349.6N848D + R880K + K921R + Y934G F20P + I302D + S313D + E408D + D476R +Y579W + S636K + 95% PUG 672 351.2 A651P + T697G + W719R + V756Y +N848D + V881T + T887K + F906A + A937E F20P + S313D + E408D + Y579W +S636K + T697G + N848D + 95% PUG 672 353.7 A937E F20P + S313D + E408D +Y579W + S616G + S636K + T697G + 95% PUG 672 356.3 W719R + T883V +N905D + A937E Y55M + A283D + S313D + E408D + Y579W + I602T + F638N + 95%PUG 672 364.7 A651P + W719R + V756Y + N848D + A869V + R880K + N905D +K921R + Y934R F20P + S313D + E408D + Y579W + S636K + A688G + T697G + 95%PUG 672 365.7 V881Q + N905D + K921R + A937E I302D + E408D + Q416S +Y579W + I602T + A651P + A688G + 95% PUG 672 367.3 W719R + K720H +R880K + N905D + F1048W Y55M + A283D + I302D + S313D + E408D + Y579W +I602T + 95% PUG 672 367.5 F638N + A651P + W719R + V756Y + N848D +A869V + R880K + K921R + Y934R F20P + S313D + E408D + Y579W + S636K +A688G + T697G + 95% PUG 672 375.4 N905D + A937E F20P + I302D + S313D +E408D + D476R + Y579W + S636K + 95% PUG 672 376.0 T697G + W719R +V756Y + V881Q + T887K + F906A + A937E Y55M + A283D + I302D + S313D +E408D + Y579W + I602T + 95% PUG 672 387.3 F638N + A651P + W719R +V756Y + N848D + A869V + R880K + N905D + K921R + Y934R A283D + S313D +E408D + Y579W + I602T + F638N + A651P + 95% PUG 672 396.0 T697G +K720H + V756Y + N848D + A869V + R880K + A885Q + K921R + Y934G F20P +I302D + S313D + E408D + D476R + Y579W + S636K + 95% PUG 672 406.2T697G + W719R + V756Y + V881Q + T887K + F906A + A937E F20P + S313D +E408D + Y579W + S636K + A688G + T697G + 95% PUG 672 409.6 W719R +A885N + N905D + A937E Y55M + A283D + I302D + S313D + E408D + Y579W +I602T + 95% PUG 672 410.3 F638N + A651P + W719R + V756Y + N848D +A869V + R880K + K921R + Y934R Y55M + A283D + S313D + E408D + Y579W +I602T + F638N + 95% PUG 168 411.7 A651P + K720H + V756Y + N848D +A869V + R880K + K921R + Y934R F20P + I302D + S313D + E408D + Y579W +S636K + A688G + 95% PUG 672 412.1 T697G + W719R + N905D + A937E N216D +I302D + H311N + E408D + Q416S + Y579W + I602T + 95% PUG 672 424.0A651P + A688G + W719R + A869V + R880K + V890R + N905D Y55M + A283D +I302D + S313D + E408D + Y579W + I602T + 95% PUG 672 426.7 F638N +A651P + W719R + V756Y + N848D + A869V + R880K + A885N + N905D + K921R +Y934R F20P + N216Q + I302D + S313D + E408D + V567P + Y579W + 95% PUG 672434.7 S636K + A651P + T697G + W719R + N848D + T883R + N905D + A937E +Q956Y + F1048W F20P + K51Q + I302D + S313D + E408D + D476R + Q489P + 95%PUG 672 440.1 AV756Y + S59N + Y579W + I602T + S636K + A651P + T697G +W719R + N848D + T883R + T887K + F906A + A937E F20P + I302D + S313D +E408D + D476R + Y579W + S636K + 95% PUG 672 442.1 T697G + W719R +V756Y + V881Q + T887K + F906A + A937E N216Q + I302D + H311N + E408D +Q416S + Y579W + I602T + 95% PUG 672 450.7 A651P + A688G + T697G +W719R + R880K + N905D + Q912V F20P + I302D + S313D + E408D + Y579W +S636K + A688G + 95% PUG 672 452.1 T697G + W719R + A885N + N905D + A937EF20P + I302D + S313D + E408D + D476R + Q489P + A559N + 95% PUG 720 452.5Y579W + I602T + S636K + A651P + T697G + W719R + V756Y + N848D + T883R +T887K + F906A + A937E F20P + I302D + S313D + A346D + E408D + D476R +Q489P + 95% PUG 672 471.0 Y579W + S636N + T697G + W719R + V756Y +A824D + N848D + V881Q + T887K + F906A + S928D + A937E + F1048W Y55M +A283D + S313D + E408D + Y579W + I602T + F638N + 95% PUG 672 471.7A651P + Y690F + T697G + W719R + V756Y + R880K + K921R + Y934G + A937EY55M + A283D + I302D + S313D + A346D + E408D + Q489P + 95% PUG 720 472.1A559P + Y579W + I602T + V603P + F638N + A651P + T697G + W719R + K744H +A824D + N848D + R880K + V881T + F906A + Q912V + K921R + Y934G + A937E +K948R + Q956Y + F1048W F20P + I302D + S313D + E408D + Y579W + S636K +A688G + 95% PUG 672 472.2 T697G + W719R + N905D + A937E N216D + S313D +E408D + D476R + Y579W + I602T + V603P + 95% PUG 720 492.4 F638N +A651P + A688G + T697G + W719R + V756H + T825G + N833D + A869V + R880K +T887K + K921R + S928D + Y934G + N941S + K948R F20P + I302D + S313D +E408D + D476R + Y579W + S636K + 95% PUG 672 498.5 A651P + T697G +W719R + V756Y + N848D + V881T + T887K + F906A + A937E F20P + I302D +S313D + E408D + D476R + Q489P + Y579W + 95% PUG 672 505.4 S636N +T697G + W719R + V756Y + A824D + V881Q + T887K + F906A + A937E + T999R +A1037E F20P + I302D + S313D + A346D + E408D + D476R + Y579W + 95% PUG672 507.2 S636N + T697G + W719R + V756Y + A824D + N848D + V881Q +T887K + F906A + A937E + T999R + F1048W N216Q + S313D + E408D + D476R +Y579W + I602T + F638N + 95% PUG 672 521.0 A651P + T697G + W719R +R880K + T887K + K921R + Y934G S17A + F20P + S313D + E408D + Y579W +I602T + A651P + 95% PUG 672 524.6 T697G + W719R + N848D + T883R +F906A + Y934G + Q956Y + T999R SF20P + I302D + S313D + E408D + D476R +Y579W + I602T + 95% PUG 672 526.8 S636K + T697G + W719R + V756Y +V881Q + T887K + F906A + A937E + T999R + F1048W F20P + I302D + S313D +A346D + E408D + D476R + Y579W + 95% PUG 672 531.0 S636N + T697G +W719R + V756Y + N848D + T883R + F906A + S928D + A937E N216D + S313D +E408D + D476R + A564E + Y579W + I602T + 95% PUG 720 552.6 F638N +A651P + Y690F + T697G + W719R + V756H + N833D + A869V + R880K + V881T +T887K + K921R + S928D + Y934G + T999R F20P + I302D + S313D + E408D +D476R + Y579W + S636K + 95% PUG 672 557.2 T697G + W719R + V756Y +N848D + A869V + V881Q + T887K + N905D + F906A + Q912V + A937E + T999R +F1048W F20P + I302D + S313D + E408D + D476R + Y579W + S636K + 95% PUG672 558.6 T697G + W719R + V756Y + N848D + A869V + V881Q + T887K +F906A + Q912V + A937E + T999R + F1048W F20P + I302D + S313D + E408D +D476R + Q489P + Y579W + 95% PUG 672 565.9 S636N + T697G + W719R +V756Y + A824D + N848D + V881Q + T887K + F906A + S928D + A937E Y55M +A283D + I302D + S313D + A386P + E408D + Q489P + 95% PUG 720 588.5A559P + Y579W + I602T + V603P + F638N + A651P + T697G + W719R + Q834E +N848D + R880K + V881T + T892P + F906A + Q912V + K921R + Y934G + A937E +F1048W F20P + I302D + S313D + E408D + Q489P + Y579W + I602T + 95% PUG720 591.8 S636N + T697G + W719R + V756Y + A824D + N848D + V881Q +T887K + N905D + F906A + S928D + A937E + T999R + A1037E + F1048W F20P +I302D + S313D + E408D + D476R + Y579W + I602T + 95% PUG 672 592.0V603P + S636K + T697G + W719R + V756Y + N848D + A869V + V881Q + T887K +F906A + Q912V + A937E + F1048W F20P + I302D + S313D + E408D + Y579W +I602T + S636K + 95% PUG 672 592.7 A688G + T697G + W719R + V756Y +V881T + N905D + A937E F20P + I302D + S313D + E408D + Q416S + D476R +Q489P + 95% PUG 672 597.1 Y579W + I602T + S636K + A651P + T697G +W719R + V756Y + A824D + N833D + N848D + T883R + T887K + F906A + A937EY55M + A283D + I302D + S313D + E408D + A559P + Y579W + 95% PUG 720 604.2I602T + V603P + F638N + A651P + T697G + W719R + N833D + N848D + R880K +V881T + F906A + Q912V + K921R + Y934G + A937E + N941S + T999R F20P +A186P + I302D + S313D + E408D + D476R + Q489P + Y579W + A599S + I602T +S636K + A651P + T697G + W719R + 95% PUG 672 616.5 V756Y + N848D +T883R + T887K + F906A + A937E Y55M + A283D + I302D + S313D + E408D +A559P + Y579W + I602T + V603P + F638N + A651P + T697G + W719R + N848D +95% PUG 720 622.2 A869V + R880K + V881T + F906A + Q912V + K921R +Y934G + A937E N216D + S313D + E408D + D476R + Y579W + I602T + V603P +95% PUG 720 622.4 F638N + A651P + A688G + T697G + W719R + V756H +R880K + T887K + K921R + S928D + Y934G + K948R F20P + A283D + I302D +S313D + E408D + Y579W + I602T + 95% PUG 672 624.4 S636K + A651P +A688G + T697G + W719R + V756Y + T887K + N905D + A937E F20P + I302D +S313D + A346D + E408D + D476R + Y579W + 95% PUG 672 629.5 I602T +T697G + W719R + N848D + V881Q + T887K + F906A + S928D + A937E + T999RF20P + K51Q + I302D + S313D + E408D + D476R + Q489P + 95% PUG 672 639.6Y579W + I602T + S636K + A651P + T697G + W719R + V756Y + N848D + T883R +T887K + F906A + A937E F20P + I302D + S313D + A346D + E408D + D476R +Q489P + 95% PUG 672 644.4 Y579W + S636N + T697G + W719R + V756Y +A824D + N848D + V881Q + T887K + F906A + A937E + T999R F20P + I302D +S313D + E408D + D476R + Q489P + Y579W + 95% PUG 672 655.2 I602T +S636N + T697G + W719R + V756Y + A824D + N848D + V881Q + T887K + N905D +F906A + A937E + T999R + A1037E + F1048W F20P + Q289E + I302D + S313D +E408D + D476R + Q489P + 95% PUG 720 658.0 A559P + Y579W + I602T +S636N + T697G + W719R + V756Y + A824D + N848D + R880K + V881Q + T887K +N905D + F906A + S928D + Y934G + T999R + A1037E + F1048W F20P + K51Q +I302D + S313D + E408D + D476R + Q489P + 95% PUG 672 658.2 Y579W +I602T + S636K + A651P + T697G + W719R + V756Y + A824D + N848D + T883R +T887K + F906A + S928D + A937E + A1037E N216D + S313D + A346D + E408D +D476R + Q489P + A559P + 95% PUG 720 662.8 Y579W + I602T + F638N +A651P + A688G + T697G + W719R + V756H + R880K + T887K + K921R + S928D +Y934G F20P + I302D + S313D + A346D + E408D + D476R + Q489P + 95% PUG 672663.4 Y579W + I602T + T697G + W719R + V756Y + N848D + V881Q + T887K +F906A + A937E N216D + S313D + E408D + D476R + Q489P + A559P + Y579W +95% PUG 720 679.1 I602T + F638N + A651P + A688G + T697G + W719R +V756H + Q834E + R880K + T887K + T892P + K921R + S928D + Y934G F20P +I302D + S313D + E408D + D476R + Q489P + A559N + 95% PUG 672 693.2Y579W + I602T + S636K + A651P + T697G + W719R + V756Y + N848D + T883R +T887K + F906A + S928D + A937E F20P + I302D + S313D + E408D + D476R +Q489P + Y579W + 95% PUG 672 701.7 I602T + S636K + A651P + T697G +W719R + V756Y + N848D + T883R + T887K + F906A + A937E F20P + I302D +S313D + E408D + Q416S + D476R + Q489P + 95% PUG 672 719.6 A559N +Y579W + I602T + S636K + A651P + T697G + W719R + V756Y + N848D + T883R +T887K + F906A + A937E F20P + I302D + S313D + E408D + D476R + Y579W +I602T + 95% PUG 672 769.2 S636N + T697G + W719R + V756Y + A824D +N848D + V881Q + T887K + F906A + S928D + A937E + T999R + F1048W

The HIFs of further purified variants tested are shown in Tables 13-16below. HIFs in Tables 14-16 were calculated in the same way as in Table13 except for a slightly increased incubation temperature for variantsand controls (26-30 degrees celcius). All half-life values of thepurified variants were calculated relative to the half-life of GH9wild-type (mature parent endoclucanase with SEQ ID NO:2) incubated atthe same detergent concentration and temperature measured as purifiedprotein. HIF of GH9 wild-type in all tables is 1 (per definition).

TABLE 13 Purified variants of the mature parent GH9 endoglucanase (SEQID NO: 2) with corresponding half-life improvement factors (HIF)incubated at 25° C. relative to a parent endoglucanase, e.g. anendoglucanase of SEQ ID NO: 2. Alteration as compared PUG (detergentIncubation to SEQ ID NO: 2 concentration) time (hrs) HIF K507R 90% PUG16 1.1 S17A 90% PUG 16 1.1 S63F 90% PUG 16 1.1 T694A 90% PUG 16 1.1F20Y + A448S + T781M 90% PUG 16 1.3 Q834E 90% PUG 16 1.3 V56M 90% PUG 161.3 I602T 90% PUG 18 1.4 A448E 90% PUG 16 1.4 A448W 90% PUG 16 1.5 F20G90% PUG 16 1.5 I403Y 90% PUG 16 1.5 S538C 90% PUG 16 1.5 E408N 90% PUG16 2.2 F20N 90% PUG 16 2.4 K512P 90% PUG 16 2.5 E408G 90% PUG 16 2.9P410G 90% PUG 16 2.9 E408A 90% PUG 16 3.0 F20P 90% PUG 16 3.3 E408P 90%PUG 16 4.2 E408S 90% PUG 16 5.4

TABLE 14 Purified variants of the mature parent GH9 endoglucanase (SEQID NO: 2) with corresponding half-life improvement factors (HIF)incubated at 26° C. relative to a parent endoglucanase, e.g. anendoglucanase of SEQ ID NO: 2. Alteration as compared PUG (detergentIncubation to SEQ ID NO: 2 concentration) time (hrs) HIF I302H 90% PUG17.25 1.1 I387T 90% PUG 19.5 1.1 K51H 90% PUG 19.5 1.1 K754R 90% PUG19.5 1.1 K390Q 90% PUG 18 1.2 S605T 90% PUG 18.5 1.3 I602D 90% PUG 18.51.3 K51Q 90% PUG 18 1.6 Y55M 90% PUG 18 1.6 K4515 90% PUG 18 1.9 Q416590% PUG 18 2.2 Q416D 90% PUG 18 2.5

TABLE 15 Purified variants of the mature parent GH9 endoglucanase (SEQID NO: 2) with corresponding half-life improvement factors (HIF)incubated at 28° C. relative to a parent endoglucanase, e.g. anendoglucanase of SEQ ID NO: 2. Alteration as compared PUG (detergentIncubation to SEQ ID NO: 2 concentration) time (hrs) HIF K388R 90% PUG5   1.3 T711Y 90% PUG 5.5 1.8 T711V 90% PUG 5.5 1.9

TABLE 16 Purified variants of the mature parent GH9 endoglucanase (SEQID NO: 2) with corresponding half-life improvement factors (HIF)incubated at 30° C. relative to a parent endoglucanase, e.g. anendoglucanase of SEQ ID NO: 2. Alteration as compared PUG (detergentIncubation to SEQ ID NO: 2 concentration) time (hrs) HIF E408D + K451590% PUG 16 26.1 E408D + A448E 90% PUG 16 36.3 E408D 90% PUG 138 53.3E408D + A448E 90% PUG 138 53.7 E408D + K4515 90% PUG 138 57.6 Y579W +E408D 90% PUG 138 72.5

Example 5 Construction and Expression of Xanthan Lyase Variants

Xanthan lyase parent gene (i.e. SEQ ID NO:5) was PCR assembled into alinear cassette containing the promoter system on the upstream and catselection maker on the downstream. To enable chromosomal integration ofthe cassette at the Pel locus of B. subtilis host by homologousrecombination, >2 kb DNA sequence identical to the site of integrationwas included on both the sides of the cassette. Genomic DNA preparedfrom the strain containing xanthan lyase parent gene (SEQ ID NO:5) wasused as template for generating the site-directed mutants. Mutagenicforward and reverse primers were used to generate an approximately 6 kbPCR fragment. This fragment was used as a megaprimer along with anotherforward primer to amplify >8 kb DNA fragment. This fragment containedthe complete cassette (promoter system, xanthan lyase and cat gene alongwith homologous DNA sequence required for recombination at Pel locus)was used for transformation.

The triple promoter system used in the cassette has been described inWO99/43835 and it consists of promoters from Bacillus licheniformisalpha-amylase gene (amyL), Bacillus amyloliquefaciens alpha-amylase gene(amyQ), and the Bacillus thuringiensis cryIIIA promoter including thestabilizing sequence. Protease signal sequence from Bacillus clausii wasincluded to export the protein out of the cells.

Generated variants of the mature parent xanthan lyase of SEQ ID NO:6 areshown in Tables 17, 18, and 19 below. The presence of the alteration wasconfirmed by sequencing.

TABLE 17 Generated variants of the mature parent xanthan lyase of SEQ IDNO: 6 Regions Alteration Region 1 (amino acids 154 to 176 of SEQ ID NO:6): Y155E A159P Region 2 (amino acids 614 to 658 of SEQ ID NO: 6): A624EA626G T631N T631E S635E S635T S635Q A645S T649V T649K T649R Q650G I656VRegion 3 (amino acids 731 to 803 of SEQ ID NO: 6): G738L K745R F746LL748T P752R P752K G753E G753Q G753S S754E S754L S754Q S754R S757D S757PS757E P764V P764K A769D A769T A769R A769S A769E A769Q A769* A774V L775ML775Y L775A L775I L775S L775F L775Q D777K D777R P779V Y782I A785T N786KG789R K792W K792Y K792V K792A N796Q A799H V800P D801G Region 4 (aminoacids 807 to 846 of SEQ ID NO: 6): K819R K819T K824R A843P D845E Region6 (amino acids 903 to 1004 of SEQ ID NO: 6): T903A T903Q A911V A911MA911S A912T A912I A912Y T915Q T915S T915V T915A T919F T919G T919D T921RT921S T923H T923D T925Q T925D T925R T927K D928W Y930H Y930L Y930F A932PD933M G941E G941D A966P A967D N991D V998K

TABLE 18 Generated variants of the mature parent xanthan lyase of SEQ IDNO: 6 Regions Alteration Region 2 (amino acids 614 to 658 of SEQ ID NO:6): T631N Region 3 (amino acids 731 to 803 of SEQ ID NO: 6): A769D A769TL775A L775F L775I L775M L775Q L7755 L775Y P779V K792A K792V K792Y N796QA799H D801G Region 5 (amino acids 872 to 885 of SEQ ID NO: 6): K875TRegion 6 (amino acids 903 to 1004 of SEQ ID NO: 6): T903A T903Q A911MA911V A912I A912T A912Y T915S T915V T919D T919G T921R T921S T923D T923HT925D T925Q T925R T927K D928W Y930F Y930H Y930L D933M G941D V998K

TABLE 19 Generated variants of the mature parent xanthan lyase of SEQ IDNO: 6 Regions Alterations Region 7 K9R (amino acids 1 to 153 of SEQ IDNO: 6) N15T L46D A58L S66H Q89Y K95E S100D N106Y Q109[R, D, F, K, A]Region 8 K183[Q, R] (amino acids 177 to 613 of SEQ ID NO: 6) V188I A190QA203P K204R A221P E229[N, S] I234V I238[W, L, M] I240W N242S G243V Y257WR258E K291R A293[G, P] K316R K320R L324Q K329R K333R L339M I341P V352IS354P K360R F377Y K400R F419Y D450P K451[E, R] A454V K481R A492L K567RG568A S578[K, R] S579[R, K] Region 9 T664K (amino acids 659 to 730 ofSEQ ID NO: 6) N672D Region 11 K855R (amino acids 847 to 871 of SEQ IDNO: 6) Region 12 K887R (amino acids 886 to 902 of SEQ ID NO: 6) N892[Y,W, F]

Bacillus organism containing a variant was inoculated in LB brothcontaining chloramphenicol (6 μg/mL) and grown overnight at 37° C. Forexpression of xanthan lyase variants, 2% of overnight culture was addedto 300 mL of 10-R medium in 1000 mL baffled flask and grown at 30° C.for 96 h at 180 rpm.

10-R medium contained 33 g/L Soluble starch, 6 g/L (NH₄)₂HPO₄, 5 g/LPotato peptone, 1.2 g/L (MgSO₄ ×7 H₂O), 12 g/L KH₂PO₄, 5 g/L (Na₂HPO₄×2H₂O), 18 mL/L of Trace metal solution, 1.8 g/L K₂SO₄ and 0.1 g/L(CaCl₂×2 H₂O and 0.5 mL/L SB2121 (anti-foam agent). Trace metal solutionwas made by mixing 0.49 g/L (MnSO₄×H₂O), 1.97 g/L (FeSO₄×7 H₂O), 0.1 g/L(CuSO₄×5 H₂O), 0.3 g/L ZnCl₂ and 19.6 g/L citric acid.

Example 6 Purification of Xanthan Lyase Variants

Prior to purification, Bacillus subtilis broth was clarified bycentrifuging at 8000×g for 30 minutes at 10° C. followed by vacuumfiltration using a combination of Seitz filter (K250) and WHATMAN glassfilter GF/F grade in a Buchner funnel. Finally, the supernatant wasfiltered through 0.22μ Tangential flow filtration unit.

Xanthan lyase variants were purified using three-step automated tandemcolumn chromatography. Macro-Prep Methyl HIC column was pre-equilibratedwith 50 mM Tris, pH 8.0 containing 1 M (NH₄)₂SO₄ and 1 mM CaCl₂ buffer.During sample loading onto the column the clarified culture supernatant(250 mL) was diluted 1:1 in-line with 50 mM Tris, pH 8.0 containing 2 M(NH₄)₂SO₄ and1 mM CaCl₂ buffer to make the final concentration to 1 M.The unbound or weakly bound protein was washed with the equilibrationbuffer until the Absorbance at 280 nm comes below 0.1 AU. Elution wascarried out using 50 mM Tris pH 8 containing 0.5 M (NH₄)₂SO₄ and 1 mMCaCl₂. Eluted protein peak was automatically loaded on MEP-Hypercelcolumn pre-equilibrated with 50 mM Tris, pH 8 containing 0.5 M (NH₄)₂SO₄and 1 mM CaCl₂. The unbound or weakly bound protein was washed with theequilibration buffer until the Absorbance at 280 nm comes below 0.1 AU.The column was washed again with 50 mM Tris, pH 8 containing 1 mM CaCl₂to remove impurities. The Purified protein was eluted with 50 mMNa-acetate, pH 5 containing 1 mM CaCl₂. The eluted purified protein wasautomatically transferred to Sephadex G-25 column pre-equilibrated with50 mM MOPS, pH 8 containing 1 mM CaCl₂ for desalting.

Example 7 Detergent Stability Assay

Reagents for the detergent stability assay were prepared as follows:

A stock of 1.0 M MOPS buffer was prepared by dissolving 209.26 g of3-Morpholinopropanesulfonic acid in Milli Q water. pH was adjusted to7.5 using NaOH and the final volume of buffer was made up to 1000 mL.This buffer stock was stored at 4° C. until use. A 50 mM workingsolution of MOPS buffer was prepared by adding 50 mL of 1.0 M stock to950 mL of Milli Q water.

A substrate solution of 0.4% w/v xanthan gum was freshly prepared bydissolving 400 mg of xanthan gum in 100 mL of Milli Q water.

A stock solution mix containing 1.0 M Na₂CO₃, 0.17 M potassium sodiumtartrate and 5 mM (Bi(NO₃)₃×5 H₂O) was prepared by dissolving 106.99 gof Na₂CO₃, 47.98 g of potassium sodium tartrate and 2.42 mg of(Bi(NO₃)₃×5 H₂O) in Milli Q water for a final volume of 1000 mL. Thisstock solution mix was filtered and stored at room temperature.

A PAHBAH reagent (1.5% PAHBAH) was freshly prepared by dissolving 1.5 gof p-hydroxybenzoic acid hydrazide (PAHBAH) in the stock solution mix.

Detergent Stability Assay:

A. Screening of Culture Supernatants of Variants

The in-detergent stability was determined by measuring the enzymaticactivity present in culture supernatants of variants or wild-typecontrols after incubation with detergent (70%, final concentration) at30° C.

Detergent stress was carried out by addition of 30 μL of culturesupernatant and 70 pμL of a Persil Universal Gel detergent (100%) intowells of 96-well microtitre plates which were shaken for 15 min at 1000rpm. Two identical plates were produced whereof one plate was incubatedat 4° C. (unstressed plate) and the other plate was incubated at 30° C.(stressed plate) for 1 h. After incubation, samples from unstressed andstressed plates were diluted 50× with dilution buffer (50 mM MOPS, 5 mMCaCl₂, pH 7.5).

To measure the enzyme activity of diluted enzyme-detergent samples,reaction mixtures were prepared in 96-well PCR plates. 50 μL of dilutedsamples were mixed with 50 μL of freshly prepared substrate solution andincubated at 40° C. for 1 h.

After incubation, 75 μL of PAHBAH reagent was added to reaction mixturein the same PCR plate and incubated in a programmable thermal cycler(T-ROBOT) for 10 min at 90° C. followed by subsequent cooling at 10° C.Samples (25 μL) were transferred to a 384 well microtitre plate and theabsorbance was measured at 405 nm using an Infinite M1000 reader (TECAN,Switzerland).

The residual activity (RA) for variants and wild-type controls wascalculated as the percentage of enzymatic activity remaining afterincubation at 30° C. relative to the enzymatic activity remaining afterincubation at 4° C., i.e. according to the following formula aftersubtracting relevant background absorbance contributions:

Residual activity (RA)=100% * Abs405 (sample incubated at 30° C)/Abs405(sample incubated at 4° C.).

The variants with higher detergent stability were picked with respect tothe wild-types grown in the plates.

B. Screening of Purified Variants

The detergent stability of purified variants was determined by measuringthe enzyme activity of the purified protein after incubation withdetergent (90%, final concentration) at 30° C.

Purified variants were diluted to a concentration of 200 ppm using 50 mMMOPS buffer. For detergent treatment, 10 μL of diluted purified sampleswere mixed with 90 μL of Persil Universal Gel detergent (100%) intowells of 96-well microtitre plates which were shaken for 20 min at 1000rpm. Two identical plates were produced whereof one plate was incubatedat 4° C. (unstressed plate) and the other plate was incubated at 30° C.(stressed plate) for 1 h. After incubation, samples from unstressed andstressed plates were diluted 50× with dilution buffer (50 mM MOPS, 1 mMCaCl₂, pH 7.5).

Enzymatic activity analysis of unstressed and stressed samples was doneas described in section A.

C. Calculating Half-Lives and Half-Life Improvement Factors (HIF)

Half-life (TV2 (in hours)) was calculated at a given detergentconcentration and storage temperature for the wild-type controls and/orvariants, as the degradation follows an exponential decay and theincubation time (hours) is known, i.e. according to the followingformulas:

T½ (variant)=(Ln (0.5)/Ln (RA-variant/100))*Time

T½ (Wild-type)=(Ln (0.5)/Ln (RA-Wild-type/100))*Time

Where “RA” is the residual activity in percent as calculated above and“Time” is the incubation time in hours.

A half-life improvement factor (HIF) under a given set of storageconditions (detergent concentration and temperature) is calculated asHIF=T½ (variant)/T½ (Wild-type), where the Wild-type is incubated underthe same storage conditions as the variant.

In cases where the difference in stability between wild-type andvariants is too large to accurately assess half-life for both wild-typeand variant using the same incubation time (see Table 24), theincubation time for wild-type and variant is different, e.g. 1 h forwild-type and up to 168 h for the most stable variants. Further, inorder to determine the stability (half-life) within a shorter durationof incubation time for the more stable variants, e.g. <168 h, theincubation temperature for some variants in Table 24 was increased by2-5 degrees Celsius.

The half-lives and calculated half-life improvement factor (HIF) valuesfor culture supernatants of single mutation variants are provided inTable 22 below. Tables 23 and 24 show the half-life for purifiedvariants having single, double or multiple mutations, as well ashalf-life improvement factor (HIF) values for the variants of Table 23.HIFs of all variants were calculated based on the wild-type xanthanlyase (SEQ ID NO:6) incubated at the same detergent contration andtemperature (HIF of wild-type=1.0 in all tables, per definition).

The obtained HIF values for the purified variants are shown in Tables20-24 below.

TABLE 20 Half-life improvement factors of purified variants: RegionsAlteration HIF — Wild-type 1 Region 1 (amino acids 154 to 176 of SEQ IDNO: 6): Y155E 1.4 Region 2 (amino acids 614 to 658 of SEQ ID NO: 6):K620R 1.5 Q650G 4.8 T631N 1.4 T649V 1.2 Region 3 (amino acids 731 to 803of SEQ ID NO: 6): K745R 2.4 S757D 3.4 G753E 4.4 G753Q 3.8 G753S 2.5S754E 2 P752R 1.4 S754L 1.3 K792W >5 Region 4 (amino acids 807 to 846 ofSEQ ID NO: 6): K819R 1.2 K824R 1.2 Region 6 (amino acids 903 to 1004 ofSEQ ID NO: 6): A966P 1.5 N991D 1.7

The obtained HIF values for culture supernatants of variants are shownin Table 21 below.

TABLE 21 Half-life improvement factors of culture supernatants ofvariants: Regions Alteration HIF — Wild-type 1 Region 2 (amino acids 614to 658 of SEQ ID NO: 6): T631N 1.2 Region 3 (amino acids 731 to 803 ofSEQ ID NO: 6): A769D 3.2 A769T 1.5 L775A >5.0 L775F 2.3 L775I >5.0L775M >5.0 L775Q 1.3 L775S >5.0 L775Y >5.0 P779V >5.0 K792A 1.9 K792V3.3 K792Y >5.0 N796Q 1.2 A799H 1.3 D801G 2.5 Region 5 (amino acids 872to 885 of SEQ ID NO: 6): K875T >5.0 Region 6 (amino acids 903 to 1004 ofSEQ ID NO: 6): T903A 1.3 T903Q 1.2 A911M 1.5 A911V 2.1 A912I 1.5 A912T1.6 A912Y 1.4 T915S 1.3 T915V 1.2 T919D 1.2 T919G 1.3 T921R 1.3 T921S1.2 T923D 1.3 T923H 2.3 T925D 1.2 T925Q 1.2 T925R 1.2 T927K 1.2 D928W2.3 Y930F 1.2 Y930H 1.2 Y930L 1.2 D933M 1.5 G941D 1.2 V998K 1.3

TABLE 22 Half-life and half-life improvement factors of culturesupernatants of variants Half- life Region Mutation (h) HIF — Wild-type0.4 1.0 Region 7 A58L 0.6 1.3 (amino acids 1 to 153 of SEQ ID NO: 6)Q89Y 0.9 2.2 Q109A 0.5 1.2 Q109D 0.6 1.3 Q109F 0.5 1.3 Q109K 0.9 2.2Region 8 K183Q 0.5 1.2 (amino acids 177 to 613 of SEQ ID NO: 6) A190Q0.5 1.2 E229N 0.8 1.9 I238L 0.6 1.4 I238W 0.7 1.6 K451E 0.6 1.3 G568A1.1 2.5 S578K 1.1 2.5 S579K 0.8 2.0 Region 9 T664K 0.5 1.1 (amino acids659 to 730 of SEQ ID NO: 6) Region 12 N892F 1.1 2.5 (amino acids 886 to902 of SEQ ID NO: 6) N892W 0.9 2.2

The obtained half-life and HIF values for purified variants tested at a70% detergent concentration (30° C., incubation time 1 h) are shown inTable 23 below.

TABLE 23 Half-life and half-life improvement factors of purifiedvariants Half-life Mutations relative to SEQ ID NO: 6 (h) HIF Wild-type0.2 1.0 F377Y 0.5 2.4 S578R 1.4 6.5 S579R 1.4 6.3 N672D 0.6 2.7 N15T 0.62.6 V188I 0.5 2.4 I238M 0.5 2.3 Y257W 0.5 2.3 L324Q 0.5 2.4 S354P 0.52.2 K204R 0.5 2.3 K291R 0.5 2.2 K316R 0.5 2.4 K320R 1.6 7.4 K329R 0.52.1 K333R 0.6 2.5 K400R 0.5 2.4 K481R 0.5 2.4 K567R 0.5 2.2 Q109R 1.88.0 K95E 0.5 2.4 S100D 0.5 2.2 R258E 0.5 2.4 N15T + S579R 1.5 6.7 K9R0.5 2.0 K183R 0.5 2.2 A293G + L324Q 1.1 4.8 N15T + K329R 0.8 3.7 L324Q +K329R 0.8 3.4 K316R + K329R 0.8 3.6 K333R + K855R 1.1 4.8 K329R + F377Y0.7 3.1 A221P + K329R 0.7 3.2 N106Y + K329R 0.7 3.2 K360R + K855R 0.83.6 K360R + F377Y 0.7 3.1 K333R + K360R 0.7 3.1 L324Q + K329R 0.7 3.1K329R + K360R 0.6 2.8 A293G + K316R 0.7 3.3 A293G + S579R 1.3 5.9Q109R + R258E 1.6 7.1 Q109R + Y257W 1.8 8.2 Q109R + I238M 2.1 9.5Q109R + K183R 1.8 8.3 S100D + K320R 0.5 2.5 S100D + Q109R 1.6 7.1 L46D +Q109R 0.9 3.9 N15T + Q109R 1.3 5.9 K451R + N672D 0.6 2.5 K451R + N892Y1.7 7.8 K451R + S578R 1.3 5.7 K451R + S579R 1.0 4.3 K451R 0.6 2.5V188I + L324Q 0.7 3.2 Q109R + A293P 2.4 10.7 Q109R + K400R 1.8 8.2Q109R + K333R 2.2 10.0

The obtained half-life values for purified variants tested at a 90%detergent concentration (temperature and incubation time as indicated)are shown in Table 24 below.

TABLE 24 Half-life values of purified variants Incubation T timeHalf-life Mutations relative to SEQ ID NO: 6 (° C.) (h) (h) Wild-type 301 0.23 V188I + K333R 30 1 0.4 V188I + L324Q 30 1 0.4 N672D + K855R 30 10.4 N242S + K329R + L339M + F377Y + S579R + N672D 30 1 0.4 I234V 30 10.5 I240W 30 1 0.5 I238M + L339M + F377Y + S579R + N672D 30 1 0.5 V352I30 1 0.5 N242S + K291R + L339M + F377Y + S579R + N672D 30 1 0.5 K360R +K567R 30 1 0.5 K316R + S579R 30 1 0.5 N242S + L339M + F377Y + K567R +S579R + N672D 30 1 0.5 L46D + Q109R 30 1 0.5 K204R + N242S + L339M +F377Y + S579R + N672D 30 1 0.5 N242S + R258E + L339M + F377Y + S579R +N672D 30 1 0.5 N242S + L324Q + L339M + F377Y + S579R + N672D 30 1 0.6E229S 30 1 0.6 D450P 30 1 0.6 A221P + N242S + L339M + F377Y + S579R +N672D 30 1 0.6 K291R + S579R 30 1 0.6 F419Y 30 1 0.6 S100D + Q109R 30 10.6 V188I + N672D 30 1 0.6 K887R 30 1 0.7 Q109R + K400R 30 1 0.7 K451R +S579R 30 1 0.8 V188I + S579R 30 1 0.8 L324Q + K360R 30 1 0.8 K291R +S578R 30 1 0.8 S100D + Q109R 30 1 0.8 A293G + S579R 30 1 0.8 Q109R +K333R 30 1 0.8 K204R + K320R 30 1 0.8 Q109R + K329R 30 1 0.8 Q109R +L324Q 30 1 0.9 S579R + K855R 30 1 0.9 K400R + K451R + N892Y 30 1 0.9K291R + N672D 30 1 0.9 Q109R + A293P 30 1 0.9 K316R + K451R + N892Y 30 10.9 N15T + Q109R 30 1 1.0 Q109R + R258E 30 1 1.0 Q109R + K183R 30 1 1.1K320R + K451R + N892Y 30 3 1.1 K451R + S578R 30 1 1.2 Q109R + Y257W 30 11.2 L46D + S579R + N892Y 30 1 1.2 Q109R + I238M 30 1 1.2 K451R + N892Y30 1 1.2 K291R + K451R + N892Y 30 1 1.3 K9R + S579R + N892Y 30 1 1.4K451R + N672D + N892Y 30 3 1.4 E229S + N672D 30 3 1.4 N892Y 30 1 1.6K95E + S579R + N892Y 30 1 1.7 K183R + E229S 30 3 1.7 F377Y + S579R +N892Y 30 1 1.7 A454V + S579R 30 3 1.7 E229S + F377Y 30 3 1.7 S100D +S579R + N892Y 30 1 1.7 L324Q + K360R + S579R 30 3 1.7 Y257W + S579R +N892Y 30 1 1.8 L324Q + S579R + N892Y 30 1 1.8 E229S + L324Q 30 3 1.8K316R + S579R + N892Y 30 1 1.8 K204R + E229S 30 3 1.8 E229S + K451R 30 31.8 N15T + S579R + N892Y 30 1 1.9 E229S + Y257W 30 3 1.9 E229S + I238M30 3 1.9 S100D + E229S 30 3 1.9 E229S + K329R 30 3 2.0 K567R + S579R +N892Y 30 1 2.0 E229S + K291R 30 3 2.0 S66H + S578R 30 3 2.0 E229S +K316R 30 3 2.0 K9R + E229S 30 3 2.0 D450P + S578R 30 3 2.0 E229S + K320R30 3 2.0 V188I + S579R + N892Y 30 1 2.0 A221P + E229S 30 3 2.0 R258E +K291R + S578R 30 3 2.1 Q109R + A454V 30 3 2.1 V188I + E229S 30 3 2.1K329R + S579R + N892Y 30 1 2.1 L46D + K291R + S578R 30 3 2.1 I238M +G243V + K291R + L339M + S578R 30 3 2.2 Q109R + K451R + N892Y 30 3 2.2A203P + K333R + S579R + N892Y 30 1 2.2 K451R + S578R + N892Y 30 3 2.2K291R + S578R + N672D 30 3 2.2 K400R + S579R + N892Y 30 1 2.2 Q109R +F419Y 30 3 2.2 K291R + K320R + S578R 30 3 2.3 Q109R + D450P 30 3 2.3K183R + K291R + S578R 30 3 2.3 K291R + S578R + N892Y 30 3 2.3 L324Q +S578R 30 3 2.3 Q109R + S578R + N892Y 30 3 2.4 K9R + K291R + S578R 30 32.4 K451R + S579R + N892Y 30 1 2.4 A221P + K291R + S578R 30 3 2.5Q109R + K360R 30 3 2.5 A221P + S579R + N892Y 30 1 2.5 K291R + F377Y +S578R 30 3 2.5 Y257W + K291R + S578R 30 3 2.6 L324Q + K360R + S578R 30 32.6 K291R + K333R + S578R 30 3 2.6 K291R + K400R + S578R 30 3 2.6K204R + S579R + N892Y 30 1 2.6 F419Y + S578R 30 3 2.7 I238M + K291R +S578R 30 3 2.7 S578R + K855R + N892Y 30 3 2.7 K291R + K567R + S578R 30 32.8 N15T + K291R + S578R 30 3 2.8 A454V + S578R 30 3 2.8 K291R + K451R +S578R 30 3 2.8 L324Q + S578R 30 3 2.9 K291R + K316R + S578R 30 3 2.9K320R + S579R + N892Y 30 1 3.0 I341P + S578R 30 3 3.0 G568A + S578R 30 33.0 K360R + S578R 30 3 3.1 K204R + K291R + S578R 30 3 3.1 V188I +K291R + S578R 30 3 3.2 S100D + K291R + S578R 30 3 3.3 Q109R + K291R +S578R 30 3 3.6 K291R + L324Q + S578R 30 3 3.6 Q109R + S579R + N892Y 30 13.7 N106Y + S579R + N892Y 30 1 4.3 E229S + S579R 30 3 5.2 Q109R + E229S30 3 5.8 N242S + L339M + F377Y + S579R + N672D + N892Y 30 1 6.6 Q109R +K887R 30 3 7.5 E229S + S578R 30 3 8.1 K204R + K291R + S578R 30 20 9.7N15T + Q109R + K887R 30 16 14 S100D + K291R + K333R + S578R 30 20 14Q109R + K183R + S579R + N892Y 30 16 15 N15T + Q109R + K291R + S578R 3020 16 Q109R + K291R + S578K 30 20 16 E229S + L339M + S578R 30 16 17E229S + S579R + N892Y 30 20 17 S100D + Q109R + S579R + N892Y 30 16 17E229S + L324Q + S578R 30 16 18 S100D + Q109R + S578K + S579R + N892Y 3020 18 Q109R + K291R + L324Q + S578R 30 20 18 Q109R + E229S + S578R 30 1618 E229S + S579R + N672D 30 20 18 K183R + E229S + S578R 30 16 18 E229S +S578R + K855R 30 16 19 E229S + S578R + K887R 30 16 19 E229S + K400R +S578R 30 16 20 Q109R + K291R + S578R + N892Y 30 20 21 E229S + S579R +K855R 30 20 16 E229S + S579R 30 20 14 Q109R + K291R + K320R + S578R 3020 14 K291R + K316R + S578R + K887R 30 20 22 Q109R + S578R + K887R 30 2023 E229S + K291R + K360R + A492L + S578R + N892Y 32 20 21 K9R + E229S +S578R 30 16 22 E229S + S578R + N892Y 32 20 23 Q109R + K291R + S578R +K887R 30 20 24 E229S + K360R + S578R 30 20 24 E229S + S578K + N892Y 3220 24 V188I + E229S + K291R + S578R 30 20 25 E229S + K360R + S578K 32 2026 E229S + S578K 32 20 26 Q109R + E229S + K291R + S578R 30 20 26 Q109R +E229S + S578K 35 70 38 Q109R 30 88 70 L46D + Q109R + E229S + S578K 35166 82 E229S + S578R + N892Y 30 88 86 Q109R 30 88 91 E229S + S578K 30 88118 S100D + E229S + K360R + S578K 30 168 137 S100D + E229S + K291R +S578R 30 168 139 E229S + S578K + N892Y 30 168 154 S100D + E229S + S578K30 88 167 E229S + S578K 30 88 176 E229S + S578K 30 168 191 E229S +A492L + S578K 30 88 212 Q109R + E229S + S578K 30 88 250

Example 8 Half-Life of Xanthan Lyase Variants

Variants of the mature parent xanthan lyase of SEQ ID NO:6 were preparedand purified as described above in Examples 5 and 6. The in-detergentstability of the variants was determined as described in Example 7 bymeasuring the enzymatic activity present in either culture supernatantsor purified samples of the variants after incubation with detergent.Incubation was performed using a 70% concentration of Persil UniversalGel detergent (PUG) at 30° C. for the culture supernatants, and a 70% or90% concentration of PUG detergent at 30° C. for the purified variants,with a variant incubation time of 1 h for the culture supernatants and 1h or 3 h for the purified variants.

The half-lives and calculated half-life improvement factor (HIF) valuesfor culture supernatants are provided in Table 25 below. Table 26 showsthe half-life and half-life improvement factors (HIF) for purifiedvariants, where HIF for variants incubated with a 70% detergentconcentration are calculated based on a wild-type half-life of 0.22 hand variants incubated with a 90% detergent concentration are calculatedbased on a wild-type half-life of 0.20 h.

TABLE 25 Half-life and half-life improvement factor (HIF) of culturesupernatants of variants Half-life Region Mutation (h) HIF — Wild-type0.42 1 Region 1 (amino acids 154 to 176 of SEQ ID NO: 6) A159P 0.5 1.2Region 2 (amino acids 614 to 658 of SEQ ID NO: 6) S635T 0.6 1.4 Region 3S754Q 0.5 1.2 (amino acids 731 to 803 of SEQ ID NO: 6) S757E 0.6 1.4S757P 0.6 1.4 A769R 0.6 1.4 A769T 1.1 2.6 L775F 1.0 2.4 L775I 1.1 2.6L775Q 0.5 1.2 L775S 1.1 2.6 T778T 0.6 1.4 Y782I 0.7 1.6 N786K 0.5 1.2G789R 0.5 1.2 K792A 0.8 1.9 K792V 1.1 2.6 K792Y 1.1 2.6 N796Q 0.5 1.2A799H 0.5 1.2 Region 5 (amino acids 872 to 885 of SEQ ID NO: 6) K875E0.6 1.4 Region 6 (amino acids 903 to 1004 of SEQ ID NO: 6) T903A 0.5 1.2T903Q 0.5 1.2 A911M 0.6 1.4 A911S 0.6 1.4 A911V 0.9 2.1 A912Y 0.6 1.4A912T 0.7 1.6 T915Q 0.6 1.4 T915S 0.5 1.2 T919D 0.5 1.2 T919F 0.5 1.2T921R 0.6 1.4 T921S 0.5 1.2 T923D 0.5 1.2 T923H 1.0 2.4 T925D 0.5 1.2T925Q 0.5 1.2 T925R 0.5 1.2 T927K 0.5 1.2 D928W 1.0 2.4 Y930F 0.5 1.2Y930H 0.5 1.2 Y930L 0.5 1.2 D933M 0.6 1.4 G941E 0.5 1.2

TABLE 26 Half-life and half-life improvement factor (HIF) of purifiedvariants Incubation Detergent time Half-life Mutations (%) (h) (h) HIFWild-type 70 1 0.22 1 Wild-type 90 1 0.2 1 K620R 70 1 0.6 2.6 T631N 70 10.6 2.5 S635E 90 1 0.7 3.3 S757D 70 1 0.6 2.9 L775A 90 3 1.6 8.1 L775Y90 1 2.2 11 L775M 90 1 3.7 19 P779V 90 1 0.8 4.2 D801G 90 1 0.6 3.1A843P 90 1 0.6 2.9 K875T 90 1 0.7 3.5 T631N + K875T 90 1 1.0 5.1 S757D +D801G 90 1 4.1 20 S757D + K875T 90 3 1.1 5.7 K875T + N991D 90 3 2.1 11

Example 9 Half-life of Xanthan Lyase Variants with Mutations inChelator-Induced Instability Regions and Adjacent Regions

Variants of the mature parent xanthan lyase of SEQ ID NO:6 were preparedand purified as described above in Examples 5 and 6. For the purposes ofthis example, variants were produced having mutations in at least onechelator-induced instability region (regions 1, 2, 3, 4, 5, 6) and in atleast one adjacent region (regions 7, 8, 9, 10, 11, 12, 13). Thein-detergent stability of the variants was determined as described inExample 7 by measuring the enzymatic activity present in purifiedsamples of the variants after incubation with detergent. Incubation wasperformed using a 70%, 90% or 95% concentration of Persil Universal Geldetergent (PUG), with incubation at a temperature of 30, 32, 35 or 37°C. and a variant incubation time ranging from 1 h to up to 840 h.

Half-lives were calculated as described above in Example 7. In caseswhere the difference in stability between wild-type and variants was toolarge to accurately assess half-life for both wild-type and variantusing the same incubation time, the incubation time for wild-type andvariant is different, e.g. 1 h for wild-type and up to 840 h for themost stable variants.

Further, in order to determine the stability (half-life) within ashorter duration of incubation time for the more stable variants, e.g.<168 h, the incubation temperature for some variants was increased by2-7° C. For variants tested at a higher temperature (i.e. >30° C.), HIFvalues based on the wild-type could not be calculated as the half-lifeof the wild-type (order of magnitude of minutes) could not be determinedaccurately at these temperatures. Stability of the variants in thetables below is therefore reported in terms of half-life (in hours).

Tables 27-33 below show the half-life for the purified variants alongwith information on the test conditions (temperature, detergentconcentration, incubation time) for each variant.

TABLE 27 Half-life of purified variants: Temperature (T) 30° C.,detergent concentration 70% Incuba- tion Half- Mutations relative to TDetergent time life SEQ ID NO: 6 (° C.) (%) (h) (h) No mutations(Wild-type) 30 70 1 0.22 K620R + K855R 30 70 1 1.0 K329R + K745R 30 70 10.8 K360R + K745R 30 70 1 0.7 A293G + K567R + S579R + 30 70 1 1.5 K620RS100D + N991D 30 70 1 0.5

TABLE 28 Half-life of purified variants: Temperature (T) 30° C.,detergent concentration 90% Incubation T Detergent time Half-lifeMutations relative to SEQ ID NO: 6 (° C.) (%) (h) (h) No mutations(Wild-type) 30 90 1 0.20 L339M + K451R + S579R + N672D + K745R + G899S30 90 1 0.4 V188I + L339M + S579R + N672D + K745R + G899S 30 90 1 0.5K291R + L339M + S579R + N672D + K745R + G899S 30 90 1 0.5 L339M +S579R + T631N + N672D + K745R + G899S 30 90 1 0.5 N242S + L339M +F377Y + S579R + T631N + N672D 30 90 1 0.5 L339M + F377Y + S579R +N672D + K745R + G899S 30 90 1 0.5 F377Y + T631N + K819R + N892Y 30 90 10.5 A221P + L339M + S579R + N672D + K745R + G899S 30 90 1 0.6 K316R +L339M + S579R + N672D + K745R + G899S 30 90 1 0.6 I238M + L339M +S579R + N672D + K745R + G899S 30 90 1 0.6 S579R + N991D 30 90 1 0.6K9R + T631N + K819R + N892Y 30 90 1 0.6 S578R + K819R 30 90 1 0.6S579R + K819R 30 90 1 0.6 N242S + L339M + F377Y + S579R + N672D +T727P + N991D 30 90 1 0.6 N242S + L339M + F377Y + S579R + N672D + S757D30 90 1 0.7 S578R + N991D 30 90 1 0.8 A293G + K567R + S579R + K620R 3090 1 0.8 Q109R + L339M + S579R + N672D + K745R + G899S 30 90 1 0.9S579R + I722F + T727P + K819R + N892Y 30 90 1 0.9 N892Y + N991D 30 90 10.9 N15T + N892Y + N991D 30 90 1 1.0 T631N + K819R + N892Y 30 90 1 1.1K451R + K620R + N892Y 30 90 1 1.1 N672D + K819R + N892Y 30 90 3 1.2S579R + T631N + A645S + N892Y 30 90 1 1.3 K819R + N892Y 30 90 1 1.3S579R + K620R + N892Y 30 90 1 1.3 N672D + K875T 30 90 3 1.3 K316R +S578R + K819R 30 90 3 1.4 N15T + T631N + K819R + N892Y 30 90 3 1.4K183R + T631N + K819R + N892Y 30 90 3 1.4 S579R + A843P 30 90 3 1.6S579R + T631N + K819R + N892Y 30 90 3 1.6 E229S + S757D 30 90 3 1.7R258E + S578R + K819R 30 90 3 1.7 S579R + S635T 30 90 3 1.8 E229S +K620R 30 90 3 1.8 S579R + K875T 30 90 3 1.8 S578R + N672D + K819R 30 903 1.8 S579R + D801G 30 90 3 1.8 S579R + T727P + N892Y + N991D 30 90 11.9 S579R + N892Y + N991D 30 90 3 1.9 A221P + S578R + K819R 30 90 3 1.9K451R + T631N + N892Y 30 90 1 1.9 N672D + D801G 30 90 1 2.0 K291R +S578R + K819R 30 90 3 2.0 I238M + S578R + K819R 30 90 3 2.1 S578R +D801G + K819R 30 90 3 2.1 S578R + D801G 30 90 3 2.1 S578R + S757D +K819R 30 90 3 2.2 Q109R + K819R + N892Y 30 90 3 2.2 V188I + S578R +K819R 30 90 3 2.2 K9R + S578R + K819R 30 90 3 2.2 L324Q + S578R + K819R30 90 3 2.3 K95E + S578R + K819R 30 90 3 2.3 S578R + K819R + N892Y +N991D 30 90 3 2.3 S578R + T631N + K819R + N892Y 30 90 3 2.3 S100D +S578R + N892Y + A967D 30 90 3 2.3 V188I + S578R + N892Y + A967D 30 90 32.3 K291R + S578R + K875T 30 90 3 2.3 K567R + S578R + K819R 30 90 3 2.4K316R + S578R + K819R 30 90 3 2.4 Q109R + T631N + K819R + N892Y 30 90 32.4 Q109R + A843P 30 90 3 2.4 K204R + S578R + K819R 30 90 3 2.4 S578R +K875T 30 90 3 2.4 Q109R + D801G 30 90 3 2.4 K291R + S578R + N991D 30 903 2.4 N106Y + S578R + K819R 30 90 3 2.5 Q109R + S635T 30 90 3 2.5K291R + S578R + D801G 30 90 3 2.5 S578R + K819R + N892Y 30 90 3 2.5K183R + S578R + K819R 30 90 3 2.5 S578R + K620R + K819R 30 90 3 2.6K291R + S578R + K620R 30 90 3 2.6 S578R + S635T 30 90 3 2.6 S578R +A843P 30 90 3 2.7 K291R + S578R + T631N 30 90 3 2.7 S578R + K819R +K875T 30 90 3 2.7 N15T + S578R + K819R 30 90 3 2.8 L339M + S578R + K819R30 90 3 2.9 K291R + S578R + S757D 30 90 3 2.9 Q109R + K875T 30 90 3 2.9F377Y + S579R + K745R 30 90 3 2.9 Q109R + S578R + K819R 30 90 3 3.7L339M + S579R + N672D + P726Q + T727P + K745R + A785T + 30 90 1 4.2N892Y + G899S S100D + R317K + S578R + K620R 30 90 1 4.5 S579R + S757D +N892Y 30 90 1 4.7 K875T + N892Y 30 90 3 9.3 N106Y + S578R + K819R +K875T 30 90 16 9.7 N15T + S578R + L775A + K819R 30 90 16 11 Q109R +N672D + K875T 30 90 16 11 Q109R + S757D + K875T 30 90 16 11 K183R +S578R + L775A + K819R 30 90 16 11 N106Y + S578R + L775A + K819R 30 90 1611 N15T + F377Y + S578R + K819R 30 90 16 12 Q109R + D801G + K819R +N892Y 30 90 16 12 K183R + S578R + K819R + K875T 30 90 16 12 Q109R +D801G + K875T 30 90 16 12 Q109R + K819R + K875T + N892Y 30 90 16 12Q109R + P779V + K819R + N892Y 30 90 16 12 Q109R + P779V + K875T 30 90 1613 V188I + K291R + S578R + L775A 30 90 20 13 I341P + S578R + L775A 30 9016 13 Q109R + F377Y + K875T 30 90 16 13 Q109R + S578R + K875T 30 90 1613 Q109R + S579R + K819R + N892Y 30 90 16 13 K291R + L324Q + S578R +K620R 30 90 20 13 Q109R + T631N + K875T 30 90 16 13 Q109R + S578R +L775A + K819R 30 90 16 13 I341P + S578R + L775M 30 90 16 13 I341P +S578R + T631N 30 90 16 14 Q109R + K875T + N991D 30 90 16 14 Q109R +K620R + K875T 30 90 16 14 K320R + S578R + L775A + K819R 30 90 16 14Q109R + L775M + K875T 30 90 16 14 Q109R + K875T + N892Y 30 90 16 15E229S + S578R + D801G 30 90 16 15 Q109R + S578R + L775A + K875T 30 90 2015 Q109R + L775M + K819R + N892Y 30 90 16 15 I341P + S578R + K875T 30 9016 16 Q109R + L775A + K819R + N892Y 30 90 16 16 Q109R + S579R + D801G +K875T 30 90 20 16 E229S + S578R + P779V 30 90 16 16 Q109R + L775A +K875T 30 90 16 16 Q109R + S578K + K819R + K875T + N892Y 30 90 20 17E229S + S578R + K875T 30 90 16 17 Q109R + L775A + P779V + K792Y +K819R + N892Y 30 90 20 18 E229S + S579R + L775A 30 90 20 18 E229S +S579R + D928W 30 90 20 18 E229S + S578R + L775A 30 90 16 18 E229S +S578R + K819R 30 90 16 18 V188I + S578R + L775A + N892Y + A967D 30 90 1619 Q109R + E229S + K819R + K875T + N892Y 30 90 20 20 Q109R + L775A +P779V + K875T + N892Y 30 90 20 20 Q109R + D801G + K819R + K875T + N892Y30 90 20 20 E229S + S578R + N991D 30 90 16 20 L339M + S578R + K819R 3090 20 21 E229S + S579R + K875T 30 90 20 21 Q109R + N892Y + N991D 30 9020 10 K320R + S578R + K819R + K875T 30 90 20 9 K183R + S578R + K819R +N892Y 30 90 20 9 Q109R + S578R + K819R + K875T 30 90 20 14 E229S +S579R + A843P 30 90 20 17 Q109R + S579R + A843P 30 90 20 12 S578R +D801G + A843P 30 90 20 10 Q109R + S578K + K875T + N892Y 30 90 20 22Q109R + L775A + P779V + K792Y + K819R + K875T + N892Y 30 90 20 22E229S + S578R + K620R 30 90 16 26 S578R + K620R + A769# 30 90 3 26Q109R + L775A + D801G + K875T 30 90 20 26 E229S + S578R + T923H 30 90 1626 Q109R + L775A + K792Y + K819R + N892Y 30 90 20 27 Q109R + L775M +K875T + N892Y 30 90 20 27 Q109R + S578K + L775A + K819R + N892Y 30 90 2027 Q109R + A769T + L775A + K792Y + K819R + N892Y 30 90 20 29 Q109R +P779V + K792Y + K875T + N892Y 30 90 20 29 L775A + K875T + N892Y 30 90 8833 Q109R + L775A + P779V + K792Y + K875T + N892Y 30 90 20 33 S578K +L775A + K875T + N892Y + A911V 30 90 90 38 Q109R + L775A + K792Y + K875T30 90 20 43 S578K + P752K + G753E + L775A + K875T + N892Y 30 90 90 46Q109R + L775A + K792Y + K875T + N892Y 30 90 20 47 S578K + L775A +K875T + N892Y + A912T 30 90 90 49 S578K + P752R + G753E + L775A +K875T + N892Y 30 90 90 49 S578K + P752R + G753E + S754E + L775A +K875T + N892Y 30 90 90 51 Q109R + L775A + K875T + N892Y 30 90 88 54S578K + G753E + S754E + L775A + K875T + N892Y 30 90 167 61 S578K +P752K + G753E + S754E + L775A + K875T + N892Y 30 90 167 62 Q109R +E229V + L775A + D801G + K875T 30 90 88 67 S578K + P752R + S754E +L775A + K875T + N892Y 30 90 167 67 S578K + A769D + L775A + K875T + N892Y30 90 90 68 S578K + L775A + D801G + K875T + N892Y 30 90 90 69 Q109R +A769T + L775A + K875T + N892Y 30 90 168 71 Q109R + S754E + A769T +L775A + K875T + N892Y 30 90 88 72 S578K + G753E + L775A + K875T + N892Y30 90 167 73 Q109R + L775A + K875T + N892Y 30 90 168 75 Q109R + A769T +L775A + K792Y + K875T + N892Y 30 90 168 78 E229S + G753E + S754E +L775A + K875T + N892Y 30 90 167 79 E229S + P752R + G753E + L775A +K875T + N892Y 30 90 167 80 E229S + P752K + G753E + L775A + K875T + N892Y30 90 167 81 S100D + L775A + D801G + K875T + N892Y 30 90 167 81 Q109R +A769T + L775A + K875T + N892Y 30 90 168 82 E229S + P752R + G753E +S754E + L775A + K875T + N892Y 30 90 167 82 S754E + L775A + D801G +K875T + N892Y 30 90 167 86 E229S + S578R + G753E + N892Y 30 90 88 86L775A + D801G + K875T + N892Y 30 90 168 86 Q109R + S578K + L775A +D801G + K875T 30 90 88 86 E229S + S578R + L775A + N892Y 30 90 88 87E229S + G753E + L775A + K875T + N892Y 30 90 167 89 Q109R + E229S +A769T + L775A + K875T + N892Y 30 90 88 95 Q109R + L775A + P779V +K792Y + D801G + K819R + 30 90 88 96 K875T + N892Y Q109R + G753E +S754E + A769T + L775A + K875T + N892Y 30 90 168 102 Q109R + P752R +G753E + S754E + A769T + L775A + 30 90 168 102 K875T + N892Y E229S +P752K + G753E + S754E + L775A + K875T + N892Y 30 90 167 103 E229S +S578R + G753E + A769D + K792Y + N892Y 30 90 168 106 P752R + S754E +L775A + D801G + K875T + N892Y 30 90 167 109 S100D + E229S + S578R +N892Y + A912T 30 90 168 109 E229S + S578R + L775A + P779V + K792Y +N892Y 30 90 168 110 E229S + S578R + P752K + S754E + K792Y + N892Y +A912T 30 90 168 112 E229S + S578R + P752R + G753E + K792Y + N892Y +A912T 30 90 168 116 E229S + L775A + D801G + K875T + N892Y 30 90 167 116Q109R + P752K + G753E + A769T + L775A + K875T + N892Y 30 90 88 117E229S + S578K + A769D + K792Y 30 90 88 119 Q109R + P752K + G753E +L775A + D801G + K875T 30 90 88 120 Q109R + G753E + A769T + L775A +K875T + N892Y 30 90 88 120 E229S + S578R + K792Y + D801G + N892Y 30 90168 120 E229S + S578K + L775A + P779V + K792Y 30 90 88 122 E229S +S578R + A769D + P779V + K792Y + N892Y 30 90 168 122 E229S + A492L +S578R + N892Y + A912T 30 90 168 122 E229S + S578R + A7695 + K792Y +N892Y 30 90 168 123 E229S + A769D + L775A + K875T + N892Y 30 90 167 125Q109R + E229S + A769T + L775A + K792Y + K875T + N892Y 30 90 168 125G753E + L775A + D801G + K875T + N892Y 30 90 168 127 E229S + F419Y +S578K + G753E 30 90 168 127 E229S + S578R + A769D + L775A + K792Y +N892Y + A912T 30 90 168 127 Q109R + E229S + P752K + G753E + A769T +L775A + 30 90 168 128 K875T + N892Y Q109R + E229S + P752K + S754E +A769T + L775A + 30 90 168 130 K875T + N892Y Q109R + E229S + G753E +A769T + L775A + K875T + N892Y 30 90 168 130 Q109R + E229S + P752R +G753E + S754E + A769T + 30 90 168 131 L775A + K875T + N892Y E229S +S578K + D801G 30 90 88 133 Q109R + P752K + G753E + S754E + A769T +L775A + 30 90 88 133 K875T + N892Y E229S + S578K + G753E + A843P 30 90168 134 E229S + S578R + L775A + K792Y + N892Y + A912T 30 90 168 137E229S + S578K + T631N + G753E 30 90 168 137 E229S + S578K + P752R +G753E 30 90 88 139 Q109R + P752R + G753E + A769T + L775A + K875T + N892Y30 90 88 139 E229S + A492L + S578K + G753E + D801G 30 90 168 140 Q109R +E229S + G753E + S754E + A769T + L775A + 30 90 168 141 K875T + N892YE229S + S578K + K875T 30 90 88 143 E229S + S578R + G753E + A769D +L775A + N892Y 30 90 167 143 G753E + L775A + D801G + K875T + N892Y 30 90167 144 E229S + S578K + G753E + N892Y 30 90 168 144 E229N + S578K +A769D + L775A + K875T + N892Y 30 90 168 145 E229S + S578K + G753E +L775A + P779V 30 90 168 145 Q109R + E229S + S578K + A769T + L775A +K875T + N892Y 30 90 168 146 E229S + S578R + P752K + K792Y + N892Y +A912T 30 90 168 149 E229S + K360R + S578K + P752K + G753E + S754E 30 90168 149 E229S + A492L + S578K + G753E 30 90 168 150 E229S + S578K +K792Y + N892Y 30 90 168 151 E229S + S578K + P779V 30 90 88 151 E229S +S578K + G753E 30 90 88 153 E229S + K360R + S578K + A769D + L775A + K792Y30 90 168 154 Q109R + E229S + T631N + G753E + S754E + A769T + L775A + 3090 168 156 K875T + N892Y Q109R + E229S + A769D + L775A + K875T + N892Y30 90 168 156 G753E + L775A + D801G + K875T + N892Y + A912T 30 90 168157 Q109R + E229S + K567R + G753E + S754E + A769T + L775A + 30 90 168158 K875T + N892Y P752R + G753E + L775A + D801G + K875T + N892Y 30 90167 158 E229S + S578K + T631N + P752K + G753E + S754E 30 90 168 159E229S + A492L + S578K + G753E + K1016T 30 90 168 159 E229S + S578K +T631N + G753E + S754E 30 90 168 159 E229S + S578K + P752R 30 90 88 160E229S + S578R + G753E + A769D + P779V + N892Y 30 90 168 160 E229S +S578K + G753E + N1008D 30 90 168 162 P752K + G753E + L775A + D801G +K875T + N892Y 30 90 167 162 E229S + S578K + G753E + A912T 30 90 168 163E229S + S578K + G753E + S754E 30 90 88 165 E229S + S578K + P752R + S754E30 90 88 165 E229S + A492L + S578K + G753E + S754E 30 90 168 167 E229S +S578K + A769D + P779V 30 90 88 168 A769D + L775A + D801G + K875T + N892Y30 90 167 172 Q109R + E229S + G753E + S754E + A769T + L775A + A843P + 3090 168 172 K875T + N892Y Q109R + E229S + S635T + G753E + S754E + A769T +L775A + 30 90 168 175 K875T + N892Y E229S + S578K + T631N + G753E +D801G 30 90 168 176 E229S + K360R + S578K + P752R + S754E + A769T +L775A + 30 90 168 178 K875T + N892Y E229S + S578K + T923H 30 90 88 178Q109R + E229S + G753E + S754E + A769T + L775A + K875T + 30 90 168 180N892Y + K1016T G753E + L775A + D801G + K875T + N892Y + V998K 30 90 168180 E229S + S578K + T631N + G753E + K1016T 30 90 168 180 Q109R + E229S +S578K + G753E + S754E + A769T + L775A + 30 90 168 182 K875T + N892YE229S + S578K + L775A + K792Y 30 90 88 184 E229S + S578K + P752K + S754E30 90 88 184 E229S + S578K + A911V + A912T + T923H 30 90 88 186 E229S +S578K + T631N + G753E + A769T + L775A 30 90 168 187 E229S + S578K +T631N + G753E + A769D + K792Y 30 90 168 188 E229S + S578K + P752K 30 9088 188 P752K + G753E + S754E + L775A + D801G + K875T + N892Y 30 90 168190 Q109R + E229S + G753E + S754E + A769T + L775A + K875T + 30 90 168191 N892Y + A932P E229S + A492L + S578K + G753E + L775A 30 90 168 195P752K + G753E + S754E + L775A + D801G + K875T + N892Y 30 90 167 195E229S + S578K + T631N + G753E + D901A 30 90 168 197 E229S + K360R +S578K + P752R + A769D + L775A + K875T + 30 90 167 209 N892Y E229S +S578K + A911V 30 90 88 210 Q109R + E229S + A769T + L775A + D801G +K875T + N892Y 30 90 168 221 E229S + A492L + S578K + G753E + N1008D 30 90168 226 E229S + A492L + S578K + G753E + L775A + P779V 30 90 168 227E229S + A492L + S578K + G753E + A769D 30 90 168 230 E229S + S578K +T631N + G753E + A769D + A774V + L775A + 30 90 168 234 P779V + K792YA769D + L775A + D801G + K875T + N892Y + V998K 30 90 168 238 E229S +K360R + S578K + S754E + A769D + L775A + K875T + 30 90 168 238 N892YE229S + S578K + A769D + L775A + K875T + N892Y 30 90 168 239 S635T +A769D + L775A + D801G + K875T + N892Y 30 90 168 243 E229S + S578K +S754E 30 90 88 243 Q109R + E229S + G753E + S754E + A769E + L775A +K875T + 30 90 168 249 N892Y E229S + A492L + S578K + G753E + L775A +K792Y 30 90 168 259 A769D + L775A + D801G + A843P + K875T + N892Y 30 90168 270 G753E + S754E + L775A + D801G + K875T + N892Y 30 90 168 276G753E + S754E + A769D + L775A + D801G + K875T + N892Y 30 90 168 277E229S + K360R + S578K + P752K + A769D + L775A + K875T + 30 90 168 280N892Y E229S + S578K + T631N + G753E + A912T 30 90 168 281 Q109R +E229S + N672D + G753E + S754E + A769T + L775A + 30 90 168 297 K875T +N892Y A190Q + A769D + L775A + D801G + K875T + N892Y 30 90 168 297P752R + G753E + A769D + L775A + D801G + K875T + N892Y 30 90 168 300P752R + G753E + S754E + A769D + L775A + D801G + K875T + 30 90 168 341N892Y A769D + L775A + D801G + K875T + N892Y + N1008D 30 90 168 343G753E + A769D + L775A + D801G + K875T + N892Y 30 90 168 344 E229S +K360R + S578K + P752R + G753E + A769D + L775A + 30 90 168 375 K875T +N892Y N672D + A769D + L775A + D801G + K875T + N892Y 30 90 168 380N672D + G753E + L775A + D801G + K875T + N892Y 30 90 168 >385 Q109R +G753E + S754E + A769T + L775A + D801G + K875T + 30 90 168 >385 N892YE229S + G753E + L775A + D801G + K875T + N892Y 30 90 168 >385 E229N +G753E + L775A + D801G + K875T + N892Y 30 90 168 >385 P752K + G753E +S754E + A769D + L775A + D801G + K875T + 30 90 168 >385 N892Y E229N +A769D + L775A + D801G + K875T + N892Y 30 90 168 >385 E229S + K360R +S578K + P752K + G753E + A769D + L775A + 30 90 168 >385 K875T + N892YE229S + A769D + L775A + D801G + K875T + N892Y 30 90 168 >385 E229S +K360R + S578K + G753E + S754E + A769D + L775A + 30 90 168 >385 K875T +N892Y Q109R + N672D + G753E + S754E + A769T + L775A + K875T + 30 90168 >385 N892Y T631N + A769D + L775A + D801G + K875T + N892Y 30 90168 >385 Q109R + E229S + G753E + S754E + A769T + L775A + D801G + 30 90168 >385 K875T + N892Y

TABLE 29 Half-life of purified variants: Temperature (T) 30° C.,detergent concentration 95% Incubation T Detergent time Half-lifeMutations relative to SEQ ID NO: 6 (° C.) (%) (h) (h) No mutations(Wild-type) 30 95 1 <0.2 E229N + N672D + P752K + G753E + A769D + L775A +D801G + 30 95 672 281 K875T + N892Y Q109R + E229S + N672D + P752R +G753E + S754E + A769T + 30 95 672 310 L775A + D801G + K875T + N892YQ109R + E229S + N672D + P752R + G753E + S754E + A769T + 30 95 672 316L775A + D801G + K875T + N892Y + D901A Q109R + A159P + E229S + N672D +P752R + G753E + S754E + 30 95 672 355 A769T + L775A + D801G + K875T +N892Y E229N + N672D + P752R + G753E + A769D + L775A + D801G + 30 95 672355 K875T + N892Y E229N + N672D + G753E + S754E + A769D + L775A +D801G + 30 95 672 356 K875T + N892Y + N1008D Q109R + E229S + G753E +S754E + A769D + L775A + D801G + 30 95 672 357 K875T + N892Y + N1008DQ109R + E229S + G753E + S754E + A769D + L775A + D801G + 30 95 672 358K875T + N892Y Q109R + E229S + N672D + G753E + S754E + A769T + L775A + 3095 672 359 D801G + K875T + N892Y + T923H Q109R + A159P + E229S + G753E +S754E + A769D + L775A + 30 95 672 366 D801G + A843P + K875T + N892YQ109R + A159P + E229S + S635E + T649K + G753E + S754E + 30 95 672 373A769D + L775A + D801G + K875T + N892Y Q109R + A190Q + E229S + N672D +G753E + S754E + A769T + 30 95 672 383 L775A + D801G + K875T + N892YQ109R + E229S + T631N + N672D + P752R + G753E + S754E + 30 95 672 383A769T + L775A + D801G + K875T + N892Y E229N + N672D + G753E + S754E +A769D + L775A + D801G + 30 95 672 384 K875T + N892Y Q109R + E229S +N672D + I703L + G753E + S754E + A769T + 30 95 672 399 L775A + D801G +K875T + N892Y E229N + P752K + G753E + A769D + L775A + D801G + A843P + 3095 672 404 K875T + N892Y Q109R + E229S + N672D + G753E + S754E + A769E +L775A + 30 95 672 405 D801G + K875T + N892Y Q109R + A159P + E229S +N672D + G753E + S754E + A769T + 30 95 672 421 L775A + D801G + A843P +K875T + N892Y Q109R + A159P + E229S + G753E + S754E + A769D + L775A + 3095 672 428 D801G + K875T + N892Y Q109R + A159P + E229S + I703L + G753E +S754E + A769D + 30 95 672 433 L775A + D801G + K875T + N892Y Q109R +A159P + E229S + K567R + N672D + G753E + S754E + 30 95 672 452 A769T +L775A + D801G + K875T + N892Y Q109R + A159P + E229S + N672D + G753E +S754E + A769T + 30 95 672 454 L775A + D801G + K875T + N892Y Q109R +E229S + T631N + N672D + P752R + G753E + S754E + 30 95 672 477 A769D +L775A + D801G + A843P + K875T + N892Y Q109R + A159P + E229S + T631N +G753E + S754E + A769D + 30 95 672 481 L775A + D801G + K875T + N892YQ109R + E229S + N672D + G753E + S754E + A769T + L775A + 30 95 672 485D801G + A843P + K875T + N892Y Q109R + E229S + N672D + I703L + P752R +G753E + S754E + 30 95 672 499 A769T + L775A + D801G + K875T + N892YQ109R + E229S + T631N + S635E + T649K + N672D + P752R + 30 95 672 513G753E + S754E + A769D + L775A + D801G + K875T + N892Y Q109R + A159P +E229S + A624E + A626G + S635E + T649K + 30 95 672 519 N672D + G753E +S754E + S757D + A769T + L775A + D801G + K875T + N892Y Q109R + A159P +E229S + S635E + T649K + N672D + I703L + 30 95 672 529 G753E + S754E +S757D + A769T + L775A + D801G + K875T + N892Y Q109R + E229S + T631N +N672D + P752R + G753E + S754E + 30 95 672 562 S757D + A769T + L775A +D801G + K875T + N892Y Q109R + E229S + T631N + N672D + P752R + G753E +S754E + 30 95 672 565 S757D + A769D + L775A + D801G + K875T + N892YE229S + 1234V + S635E + T649K + I656V + N672D + G753E + 30 95 672 611A769D + L775A + D801G + K875T + N892Y Q109R + E229S + T631N + N672D +P752R + G753E + S754E + 30 95 672 637 S757D + A769D + L775A + D801G +K875T + N892Y + K1016T E229S + S635E + T649K + I656V + N672D + S754E +A769D + 30 95 672 662 L775A + D801G + K875T + N892Y E229S + A624E +S635E + T649K + 1656V + N672D + I703L + 30 95 672 663 G753E + S754E +A769D + L775A + D801G + K875T + N892Y + N1008D E229S + N440K + S582K +N672D + G753E + S754E + A769D + 30 95 672 691 L775A + D801G + K875T +N892Y + N1008D T18D + Q109R + E229S + T631N + N672D + P752R + G753E + 3095 840 694 S754E + S757D + A769D + L775A + D801G + K875T + N892Y E229S +N440K + S582K + T631N + N672D + G753E + S754E + 30 95 840 713 A769D +L775A + D801G + K875T + N892Y + N1008D L46D + E229S + K360G + D458S +S582K + N672D + G753E + 30 95 840 714 S754E + A769D + L775A + D801G +K875T + N892Y + N1008D Q109R + E229S + K567R + T631N + N672D + P752R +G753E + 30 95 840 732 S754E + S757D + A769D + L775A + D801G + K875T +N892Y S100D + E229S + S635E + T649K + 1656V + N672D + I703L + 30 95 672754 G753E + A769D + L775A + D801G + A843P + K875T + N892Y + N1008DE229S + D458S + K567R + T631N + N672D + G753E + S754E + 30 95 672 780A769D + L775A + D801G + K875T + N892Y E229S + D458S + S582K + N672D +G753E + S754E + A769D + 30 95 672 783 L775A + D801G + K875T + N892Y +N1008D E229S + S635E + T649K + I656V + N672D + G753E + S754E + 30 95 672796 A769D + L775A + D801G + K875T + N892Y E229S + N672D + G753E +S754E + A769D + L775A + D801G + 30 95 672 827 K875T + N 892Y Q109R +E229S + K360G + T631N + N672D + P752R + G753E + 30 95 840 831 S754E +S757D + A769D + L775A + D801G + K875T + N892Y E229N + T631N + N672D +G753E + S754E + A769D + L775A + 30 95 672 834 D801G + K875T + N892Y +N1008D E229S + S635E + T649K + I656V + N672D + P752R + G753E + 30 95 672836 S754E + A769D + L775A + D801G + K875T + N892Y E229S + I234V +N672D + G753E + S754E + A769D + L775A + 30 95 840 844 D801G + K875T +N892Y E229S + S635E + T649K + I656V + N672D + G753E + A769D + 30 95 672857 L775A + D801G + K875T + N892Y E229S + I234V + A492L + S582K +N672D + M728V + G753E + 30 95 840 857 S754E + A769D + L775A + D801G +K875T + N892Y E229S + S635E + T649K + I656V + N672D + G753E + S754E + 3095 840 868 A769D + L775A + D801G + K875T + N892Y E229S + S635E + T649K +I656V + N672D + G753E + A769D + 30 95 672 943 L775A + D801G + K875T +N892Y A159P + E229S + N440K + N672D + G753E + S754E + A769D + 30 95 672946 L775A + D801G + K875T + N892Y E229S + D458S + N672D + G753E +S754E + A769D + L775A + 30 95 840 950 D801G + K875T + N892Y E229S +D458S + T631N + N672D + G753E + S754E + S757D + 30 95 672 956 A769D +L775A + D801G + K875T + N892Y S100D + E229S + S635E + T649K + 1656V +N672D + P752K + 30 95 672 956 G753E + S754E + A769D + L775A + D801G +K875T + N892Y E229S + D458S + T631E + N672D + G753E + S754E + A769D + 3095 840 961 L775A + D801G + K875T + N892Y E229S + S582K + N672D + G753E +S754E + A769D + L775A + 30 95 672 966 D801G + K875T + N892Y E229S +S635E + T649K + I656V + N672D + P752K + G753E + 30 95 672 978 S754E +A769D + L775A + D801G + K875T + N892Y E229S + D458S + S582K + T631E +N672D + G753E + S754E + 30 95 840 993 A769D + L775A + D801G + K875T +N892Y A190Q + E229S + S635E + T649K + 1656V + N672D + I703L + 30 95 6721004 G753E + S754E + S757D + A769D + L775A + D801G + K875T + N892Y +N1008D A190Q + E229S + S635E + T649K + 1656V + N672D + I703L + 30 95 6721015 G753E + S754E + A769D + L775A + V800P + D801G + K875T + N892Y +N1008D A190Q + E229S + I234V + S582K + N672D + G753E + S754E + 30 95 6721019 A769D + L775A + D801G + K875T + N892Y T18D + E229S + S582K +N672D + G753E + S754E + A769D + 30 95 840 1023 L775A + D801G + K875T +N892Y E229S + D458S + T631E + N672D + G753E + S754E + S757D + 30 95 8401030 A769D + L775A + D801G + K875T + N892Y Q89Y + E229S + N672D +G753E + S754E + A769D + L775A + 30 95 840 1038 D801G + K875T + N892YS100D + E229S + D458S + K567R + S635E + N672D + G753E + 30 95 672 1041S754E + A769D + L775A + D801G + K875T + N892Y E229S + S635E + T649K +1656V + N672D + I703L + G753E + 30 95 840 1045 S754E + A769D + L775A +D801G + K875T + N892Y + N1008D E229S + V3521 + S635E + T649K + 1656V +N672D + G753E + 30 95 840 1065 S754E + A769D + L775A + V800P + D801G +K875T + N892Y E229S + N672D + P752R + G753E + S754E + A769D + L775A + 3095 672 1066 D801G + K875T + N892Y E229S + K360G + D458S + S582K +N672D + G753E + S754E + 30 95 840 1067 A769D + L775A + D801G + K875T +N892Y + N1008D E229S + I234V + A492L + N672D + G753E + S754E + A769D +30 95 840 1070 L775A + D777R + D801G + K875T + N892Y E229S + S635E +T649K + N672D + G753E + S754E + A769D + 30 95 840 1081 L775A + D801G +K875T + N892Y + N1008D E229S + N440K + S582K + A624E + N672D + P752R +G753E + 30 95 624 1087 S754E + A769D + L775A + D801G + K875T + N892Y +N1008D E229S + I234V + S582K + N672D + G753E + S754E + A769D + 30 95 7921094 L775A + V800P + D801G + K875T + N892Y T18D + E229S + S582K +N672D + G753E + S754E + A769D + 30 95 672 1101 L775A + D801G + K875T +N892Y + T902F Q89Y + E229S + N440K + S582K + A624E + N672D + G753E + 3095 672 1117 S754E + A769D + L775A + D801G + K875T + N892Y E229S +D458S + T631E + N672D + G753E + S754E + A769D + 30 95 840 1121 L775A +D777K + D801G + K875T + N892Y E229S + S635E + T649K + I656V + N672D +P752K + G753E + 30 95 672 1141 A769D + L775A + D801G + A843P + K875T +N892Y E229S + S635E + T649K + I656V + N672D + G753E + S754E + 30 95 6721145 S757D + A769D + L775A + D801G + K875T + N892Y E229S + N440K +S582K + N672D + G753E + S754E + A769D + 30 95 672 1146 L775A + D801G +A843P + K875T + N892Y + N1008D S100D + E229S + S635E + T649K + 1656V +N672D + G753E + 30 95 672 1153 S754E + A769D + L775A + D801G + K875T +N892Y L46D + E229S + K360R + S578K + N672D + G753E + S754E + 30 95 8401172 A769D + L775A + D801G + K875T + N892Y E229S + T631N + N672D +I703L + P752K + G753E + A769D + 30 95 840 1183 L775A + D801G + A843P +K875T + N892Y E229S + A624E + S635E + T649K + I656V + N672D + G738L + 3095 792 1192 G753E + 5754R + S757D + A769D + L775A + D777K + D801G +K875T + N892Y E229S + N440K + S582K + A624E + N672D + G753E + S754E + 3095 624 1214 A769D + L775A + V800P + D801G + K875T + N892Y E229S +D458S + K567R + S635E + N672D + G753E + S754E + 30 95 624 1226 A769D +L775A + D801G + K875T + N892Y A190Q + E229S + S635E + T649K + 1656V +N672D + I703L + 30 95 672 1238 P752R + G753E + S754E + A769D + L775A +D801G + K875T + N892Y + N1008D E229S + T631N + N672D + I703L + P752K +G753E + S754E + 30 95 840 1259 A769D + L775A + D801G + K875T + N892YA190Q + E229S + T631N + N672D + I703L + P752K + G753E + 30 95 840 1269A769D + L775A + D801G + K875T + N892Y A190Q + E229S + S635E + T649K +I656V + N672D + P752K + 30 95 672 1282 G753E + A769D + L775A + D801G +A843P + K875T + N892Y A159P + A190Q + E229S + I234V + S582K + N672D +G753E + 30 95 672 1289 S754E + A769D + L775A + D801G + K875T + N892YE229S + A492L + S635E + T649K + I656V + N672D + P752R + 30 95 672 1298G753E + S754E + A769D + L775A + D801G + K875T + N892Y + N1008D A190Q +E229S + S582K + N672D + G753E + S754E + A769D + 30 95 792 1299 L775A +D801G + K875T + N892Y T18D + E229S + S582K + N672D + G753E + S754E +P764K + 30 95 624 1299 6A79D + L775A + D801G + K875T + N892Y E229S +N440K + S582K + N672D + P752R + G753E + S754E + 30 95 624 1328 7S57D +A769D + L775A + D801G + K875T + N892Y + N1008D E229S + A492L + S635E +T649K + I656V + N672D + G753E + 30 95 840 1329 A769D + L775A + D801G +K875T + N892Y E229S + S582K + S635E + N672D + P752R + G753E + S754E + 3095 792 1352 A769D + L775A + D801G + K875T + N892Y + N1008D A190Q +E229S + N440K + S582K + A624E + S635E + N672D + 30 95 672 1358 G753E +S754E + A769D + L775A + D801G + K875T + N892Y A190Q + E229S + D458S +T631N + N672D + G753E + S754E + 30 95 624 1398 A769D + L775A + D801G +K875T + N892Y E229S + I234V + A492L + S582K + N672D + G753E + S754E + 3095 840 1426 A769D + L775A + D801G + K875T + N892Y E229S + N440K +S582K + S635E + N672D + G753E + S754E + 30 95 840 1456 A769D + L775A +D801G + A843P + K875T + N892Y + N1008D S100D + A190Q + E229S + 1234V +S582K + N672D + G753E + 30 95 672 1460 S754E + A769D + L775A + D801G +K875T + N892Y A190Q + E229S + K360G + D458S + S582K + T664K + N672D + 3095 792 1481 G753E +S754E + A769D + L775A + D801G + K875T + N892Y +N1008D S100D + E229S + N440K + S582K + N672D + P752R + G753E + 30 95 8401489 S754E + A769D + L775A + D801G + K875T + N892Y + N1008D E229S +S635E + T649K + I656V + N672D + P752K + G753E + 30 95 672 1519 S757D +A769D + L775A + D801G + A843P + K875T + N892Y S100D + E229S + K360G +D458S + S582K + N672D + G753E + 30 95 792 1530 S754E + S757D + A769D +L775A + D801G + A843P + K875T + N892Y + T915A + N1008D E229S + N440K +S582K + A624E + S635E + N672D + G738L + 30 95 792 1535 G753E + S754E +S757D + A769D + L775A + D801G + K875T + N892Y S100D + E229S + K360G +D458S + S582K + N672D + G753E + 30 95 840 1538 S754E + A769D + L775A +D801G + K875T + N892Y + N1008D A190Q + E229S + D458S + T631N + N672D +G753E + S754E + 30 95 672 1551 A769D + L775A + D801G + A843P + K875T +N892Y A190Q + E229S + K360G + D458S + S582K + N672D + G753E + 30 95 8401556 S754E + A769D + L775A + D801G + K875T + N892Y + N1008D E229S +S582K + S635E + T649K + I656V + N672D + M728V + 30 95 672 1594 G753E +5754R + S757D + A769D + L775A + D801G + K875T + N892Y A190Q + E229S +N440K + S582K + A624E + S635E + N672D + 30 95 792 1601 G753E + S754E +S757D + A769D + L775A + D801G + K875T + N892Y S100D + A190Q + E229S +S635E + T649K + 1656V + N672D + 30 95 624 1606 I703L + G753E + S754E +A769D + L775A + D801G + K875T + N892Y + N1008D S100D + E229S + D458S +K567R + S582K + S635E + N672D + 30 95 792 1612 G753E + S754E + A769D +L775A + D801G + K875T + N892Y E229S + S582K + S635E + T649K + I656V +N672D + P752K + 30 95 624 1619 G753E + S757D + A769D + L775A + D801G +A843P + K875T + N892Y E229S + S635E + T649K + I656V + N672D + G753E +S754R + 30 95 672 1664 S757D + A769D + L775A + D801G + A843P + K875T +N892Y T18D + E229S + D458S + T631N + N672D + M728V + G753E + 30 95 7921700 S754E + S757D + A769D + L775A + D801G + K875T + N892Y E229S +D458S + S582K + T631N + S635E + N672D + M728V + 30 95 792 1704 G753E +S754E + S757D + A769D + L775A + D801G + K875T + N892Y A190Q + E229S +K360G + D458S + S582K + N672D + G753E + 30 95 792 1714 S754E + S757D +A769D + L775A + D801G + K875T + N892Y + N1008D S100D + E229S + K360G +D458S + S582K + N672D + G753E + 30 95 792 1741 S754E + S757D + A769D +L775A + D801G + A843P + K875T + N892Y + N1008D E229S + S635E + N672D +P752R + G753E + S754E + A769D + 30 95 792 1745 L775A + D777K + D801G +K875T + N892Y A159P + E229S + D458S + T631N + N672D + M728V + G753E + 3095 792 1783 S754E + S757D + A769D + L775A + D801G + K875T + N892YE229S + A492L + S635E + T649K + I656V + N672D + G753E + 30 95 792 1826S757D + A769D + L775A + D801G + K875T + N892Y S100D + A190Q + E229S +K360G + D458S + S582K + N672D + 30 95 792 1844 G753E + S754E + A769D +L775A + D801G + K875T + N892Y + N1008D S100D + E229S + N440K + S582K +T631N + N672D + G753E + 30 95 624 1907 S754E + A769D + L775A + D801G +K875T + N892Y + N1008D E229S + I234V + A492L + N672D + G753E + S754E +A769D + 30 95 624 1924 L775A + D777K + D801G + K875T + N892Y A190Q +E229S + I234V + S582K + N672D + G753E + S754E + 30 95 672 1993 S757D +A769D + L775A + D801G + K875T + N892Y E229S + D458S + A492L + T631N +N672D + G753E + S754E + 30 95 624 2378 S757D + A769D + L775A + D801G +K875T + N892Y E229S + A624E + S635E + T649K + I656V + N672D + G753E + 3095 624 2380 S754R + S757D + A769D + L775A + D777K + D801G + K875T +N892Y S100D + E229S + K360G + D458S + S582K + N672D + G753E + 30 95 7923039 S754E + S757D + A769D + L775A + D801G + A843P + K875T + N892Y +N1008D E229S + D458S + K567R + S582K + S635E + T649K + N672D + 30 95 840674 G753E + S754E + A769D + L775A + D777R + D801G + K875T + N892YS100D + E229S + K360G + D458S + S582K + T664K + N672D + 30 95 840 1023G753E + 5754R + S757D + A769D + L775A + D801G + A843P + K875T + N892Y +N1008D + K1016T S100D + E229S + K360G + D458S + S582K + T664K + N672D +30 95 840 963 G753E + S754E + S757D + A769D + L775A + D801G + A843P +K875T + N892Y + N1008D + K1016T S100D + E229S + K360G + D458S + S582K +N672D + G753E + 30 95 840 982 S754E + S757D + A769D + L775A + D801G +A843P + K875T + N892Y + A911V + N1008D S100D + E229S + K360G + D458S +S582K + N672D + G753E + 30 95 840 906 S754E + S757D + A769D + L775A +D801G + A843P + K875T + N892Y + A911V + N1008D + K1016T S100D + E229S +K360G + D458S + S582K + S635E + N672D + 30 95 840 918 G753E + S754E +S757D + A769D + L775A + D801G + A843P + K875T + N892Y + N1008D S100D +E229S + K360G + D458S + S582K + T664K + N672D + 30 95 840 962 G753E +S754E + S757D + A769D + L775A + D801G + A843P + K875T + N892Y + A911V +N1008D + K1016T S100D + E229S + K360G + D458S + S582K + N672D + G753E +30 95 840 888 S754E + S757D + A769D + L775A + D801G + A843P + K875T +N892Y + N1008D + K1016T S100D + E229S + K360G + D458S + S582K + T664K +N672D + 30 95 840 1091 G753E + S754E + S757D + A769D + L775A + D801G +A843P + K875T + N892Y + A911V + N1008D + K1016T S100D + E229S + K360G +D458S + S582K + N672D + G753E + 30 95 840 796 S754E + S757D + A769D +L775A + D801G + A843P + K875T + N892Y + N1008D + K1016T S100D + E229S +K360G + D458S + S582K + S635E + T649K + 30 95 840 984 N672D + G753E +S754E + S757D + A769D + L775A + D801G + A843P + K875T + N892Y + N1008DS100D + E229S + K360G + D458S + S582K + N672D + G753E + 30 95 840 867S754E + S757D + A769D + L775A + D801G + A843P + K875T + N892Y + N1008DS100D + E229S + K360G + D458S + S582K + N672D + G753E + 30 95 840 876S754E + S757D + A769D + L775A + D801G + A843P + K875T + N892Y E229S +N440K + S582K + A624E + S635E + N672D + G738L + 30 95 840 689 G753E +S754E + S757D + A769D + L775A + D801G + K875T + N892Y E229S + N399K +D458S + A492H + K567R + S582K + S635E + 30 95 840 2256 T649K + N672D +G753E + S754E + A769D + L775A + D777R + D801G + K875T + N892Y A190Q +E229S + I234V + T505I + S582K + N672D + G753E + 30 95 840 698 S754E +S757D + A769D + L775A + D801G + K875T + N892Y E229S + D458S + A492H +K567R + S582K + S635E + T649K + 30 95 840 492 N672D + G753E + S754E +A769D + L775A + D777R + D801G + K875T + N892Y E229S + N399K + D458S +K567R + S582K + S635E + N672D + 30 95 840 3540 G753E + S754E + A769D +L775A + D777R + D801G + K875T + N892Y E229S + D458S + A492H + K567R +S582K + S635E + N672D + 30 95 840 2837 G753E + S754E + A769D + L775A +D777R + D801G + K875T + N892Y E229S + N399K + D458S + A492H + K567R +S582K + S635E + 30 95 840 536 N672D + G753E + S754E + A769D + L775A +D777R + D801G + K875T + N892Y E229S + N399K + D458S + K567R + S582K +S635E + T649K + 30 95 840 2830 N672D + G753E + S754E + A769D + L775A +D777R + D801G + K875T + N892Y A190Q + E229S + I234V + S582K + N672D +G753E + S754E + 30 95 840 874 S757D + A769D + L775A + D801G + K875T +N892Y E229S + N440K + S582K + A624E + S635E + N672D + G738L + 30 95 840768 G753E + S754E + S757D + A769D + L775A + D801G + K875T + N892YS100D + E229S + K360G + D458S + S582K + N672D + G753E + 30 95 840 883S754E + S757D + A769D + L775A + D801G + A843P + K875T + N892Y + N1008DE229S + N399K + D458S + A492H + K567R + S582K + S635E + 30 95 840 1836T649K + N672D + G753E + S754E + A769D + L775A + D777R + D801G + K875T +N892Y

TABLE 30 Half-life of purified variants: Temperature (T) 32° C.,detergent concentration 90% Incubation Half- T Detergent time lifeMutations relative to SEQ ID NO: 6 (° C.) (%) (h) (h) No mutations(Wild-type) 32 90 1 <0.2 Q109R + A769T + L775A + K792Y + K875T 32 90 2012 L775A + K875T + N892Y + A911V + D933M 32 90 20 13 Q109R + L775A +K792Y + K875T 32 90 20 13 L775A + K875T + N892Y 32 90 20 14 L775A +S851F + K875T + N892Y + A911V 32 90 20 14 L775A + K875T + N892Y +A911V + A912T + T923H 32 90 20 14 L775A + K875T + N892Y + D933M 32 90 2015 E229S + S578R + A769D + L775A + K819R 32 90 20 16 E229S + K291R +S578R + P752R + G753E 32 90 20 17 E229S + L775A + K875T + N892Y 32 90 2017 E229S + K291R + S578R + G753E 32 90 20 17 Q109R + S578K + D801G +K875T 32 90 20 17 E229S + S578R + L775A + K819R 32 90 20 18 E229S +S578R + D801G + K819R 32 90 20 18 E229S + S578R + K792Y + K819R 32 90 2018 Q109R + L775A + K792Y + K875T + N892Y 32 90 20 19 E229S + S578R +A769D + K792Y + K819R 32 90 20 19 S578K + L775A + K875T + N892Y 32 90 2020 E229S + S578R + P752R + G753E + N892Y 32 90 20 20 E229S + S578R +S754E + N892Y 32 90 20 20 E229S + S578R + L775A + N892Y 32 90 20 21E229S + S578R + L775A + K792Y + K819R 32 90 20 21 E229S + S578R +G753E + N892Y 32 90 20 21 Q109R + L775A + K875T + N892Y 32 90 20 21E229S + K291R + S578R + P752K + G753E 32 90 20 21 E229S + K291R +S578R + P752R 32 90 20 21 E229S + S578K + K819R 32 90 20 22 E229S +S578R + P779V + K819R 32 90 20 22 E229S + S578R + K819R 32 90 20 23Q109R + A769T + L775A + K792Y + D801G + K819R + N892Y 32 90 20 23Q109R + A769T + L775A + D801G + K819R + N892Y 32 90 20 25 E229S +S578R + P752K + G753E + N892Y 32 90 20 25 E229S + S578R + A769Q + K819R32 90 20 25 E229S + S578R + P752K + S754E + N892Y 32 90 20 27 Q109R +L775A + K875T + N892Y 32 90 20 28 Q109R + A769T + L775A + K792Y +K875T + N892Y 32 90 20 31 Q109R + A769T + L775A + K875T + N892Y 32 90 2033 L775A + D801G + K875T + N892Y 32 90 20 36 Q109R + L775A + P779V +K792Y + D801G + K819R + K875T + N892Y 32 90 20 37 Q109R + A769T +L775A + K875T + N892Y 32 90 20 38 Q109R + E229S + A769T + L775A +K875T + N892Y 32 90 20 45 Q109R + P752R + G753E + A769T + L775A +K875T + N892Y 32 90 20 55 Q109R + P752K + G753E + S754E + A769T +L775A + K875T + N892Y 32 90 20 56 Q109R + S754E + A769T + L775A +K875T + N892Y 32 90 20 58 Q109R + P752R + G753E + S754E + A769T +L775A + K875T + N892Y 32 90 20 58 Q109R + P752K + G753E + A769T +L775A + K875T + N892Y 32 90 20 60 Q109R + G753E + A769T + L775A +K875T + N892Y 32 90 20 61 Q109R + G753E + S754E + A769T + L775A +K875T + N892Y 32 90 20 62 Q109R + N672D + G753E + S754E + A769T +L775A + K875T + N892Y 32 90 167 95 E229S + K360R + S578K + P752R +A769D + L775A + K875T + 32 90 167 98 N892Y + A911V E229S + K360R +S578R + N672D + P752R + A769D + L775A + 32 90 167 98 K875T + N892YN672D + G753E + L775A + D801G + K875T + N892Y + A911V 32 90 167 108E229S + K360R + S578K + N672D + P752R + A769D + L775A + 32 90 167 116K875T + N892Y N672D + A769D + L775A + D801G + K875T + N892Y 32 90 167120 T631N + N672D + A769D + L775A + D801G + K875T + N892Y 32 90 167 122N672D + G753E + L775A + D801G + K875T + N892Y + N1008D 32 90 167 123A190Q + N672D + A769D + L775A + D801G + K875T + N892Y 32 90 167 124Q109R + G753E + S754E + A769T + L775A + D801G + K875T + N892Y 32 90 167126 Q89Y + N672D + A769D + L775A + D801G + K875T + N892Y 32 90 167 129N672D + G753E + L775A + D801G + A843P + K875T + N892Y 32 90 167 131L46D + E229S + K360R + S578K + N672D + P752R + A769D + 32 90 167 131L775A + K875T + N892Y E229S + K360R + S578K + N672D + P752R + A769D +L775A + 32 90 167 133 K875T + N892Y E229S + K360R + S578K + N672D +P752R + A769D + L775A + 32 90 167 135 K875T + N892Y + A912T E229S +K360R + S578K + P752K + G753E + S754E + A769D + 32 90 167 136 L775A +K875T + N892Y E229S + S578K + A769D + L775A + K875T + N892Y 32 90 167138 E229S + S578K + P752K + S754E + K875T + N892Y 32 90 167 140 E229S +K567R + S578K + A769D + L775A + K875T + N892Y 32 90 167 141 E229S +K360R + S578K + P752R + A769D + L775A + D801G + 32 90 167 155 K875T +N892Y N672D + G753E + L775A + D801G + K875T + N892Y 32 90 167 160E229S + S578K + A769D + L775A + P779V + K792Y + K875T + N892Y 32 90 167167 E229S + K360R + S578K + T631N + N672D + P752R + A769D + 32 90 167168 L775A + K875T + N892Y N672D + A769D + L775A + D801G + A843P +K875T + N892Y 32 90 167 171 E229S + K360R + S578K + N672D + P752R +A769D + L775A + 32 90 167 175 K875T + N892Y + A932P E229S + A492L +S578K + T631N + G753E 32 90 167 176 E229S + S578K + P752K + G753E +A769D + L775A + K875T + 32 90 167 182 N892Y E229S + K360R + S578K +N672D + P752R + G753E + A769D + 32 90 167 185 L775A + K875T + N892YN672D + G753E + S754E + A769D + L775A + D801G + K875T + N892Y 32 90 167187 E229S + S578K + P752K + G753E + S754E + A769D + L775A + 32 90 167196 K875T + N892Y E229S + K360R + S578K + N672D + P752R + G753E +S754E + 32 90 167 205 A769D + L775A + K875T + N892Y E229S + K360R +S578K + N672D + P752K + G753E + S754E + 32 90 167 216 A769D + L775A +K875T + N892Y E229S + S578K + G753E + A769D + L775A + K875T + N892Y 3290 167 218 E229S + K360R + S578K + N672D + G753E + S754E + A769D + 32 90167 221 L775A + D845E + K875T + N892Y Q109R + E229S + S578K + P752K 3290 167 225 E229S + S578K + G753E + S754E + A769D + L775A + K875T + N892Y32 90 167 234 E229S + K360R + S578K + N672D + P752K + G753E + A769D + 3290 167 234 L775A + K875T + N892Y E229S + S578K + N672D + A769D + L775A +K875T + N892Y 32 90 167 239 E229S + S578K + P752R + G753E + S754E +A769D + L775A + 32 90 167 278 K875T + N892Y Q109R + E229S + G753E +S754E + A769T + L775A + D801G + 32 90 167 308 K875T + N892Y E229S +S578K + A769D + L775A + D801G + K875T + N892Y 32 90 167 337 E229S +N672D + G753E + L775A + D801G + K875T + N892Y 32 90 167 367 E229N +N672D + A769D + L775A + D801G + K875T + N892Y 32 90 167 >385 E229S +N672D + A769D + L775A + D801G + K875T + N892Y 32 90 167 >385

TABLE 31 Half-life of purified variants: Temperature (T) 35° C.,detergent concentration 90% Incubation Half- T Detergent time lifeMutations relative to SEQ ID NO: 6 (° C.) (%) (h) (h) No mutations(Wild-type) 35 90 1 <0.2 E229N + P752R + G753E + S754E + L775A + D801G +K875T + N892Y 35 90 71 27 E229N + G753E + L775A + D801G + K875T + N892Y35 90 71 27 Q109R + E229S + K451R + A769T + L775A + D801G + K875T +N892Y 35 90 70 28 E229S + S578K + G753E + A912T 35 90 70 28 T631N +P752R + G753E + S754E + A769D + L775A + D801G + 35 90 71 30 E845D +K875T + N892Y E229S + K360R + S578K + P752R + S754E + A769D + L775A + 3590 71 30 K875T + N892Y Q89Y + Q109R + E229S + A769T + I775A + D801G +K875T + N892Y 35 90 70 30 N672D + A769D + L775A + D801G + K875T + N892Y35 90 70 33 E229N + A769D + L775A + D801G + K875T + N892Y 35 90 71 33Q109R + N672D + G753E + S754E + A769T + L775A + K875T + N892Y 35 90 7033 Q109R + E229S + S578K + P779V 35 90 70 34 N672D + G753E + L775A +D801G + K875T + N892Y + A911V 35 90 70 34 E229S + S578K + G753E + A912T35 90 70 34 S100D + Q109R + N672D + G753E + S754E + A769T + L775A + 3590 70 35 K875T + N892Y E2295 + K360R + S578K + S635E + T649K + P752R +A769D + 35 90 71 38 L775A + D801G + K875T + N892Y Q109R + G753E +S754E + A769E + L775A + D801G + K875T + N892Y 35 90 71 38 E2295 +K360R + S578K + N672D + P752R + S754E + A769D + 35 90 71 40 L775A +K875T + N892Y Q109R + E2295 + S578K + A912T 35 90 70 40 E2295 + K360R +S578K + P752R + A769D + L775A + D801G + 35 90 71 42 K875T + N892YQ109R + A159P + E2295 + N672D + F746L + G753E + S754E + 35 90 168 42A769T + L775A + D801G + A843P + K875T + N892Y E2295 + S578K + G753E +P779V + K792Y + D801G + A912T 35 90 70 42 E2295 + K360R + S578K +P752R + S754E + A769D + L775A + 35 90 71 44 D801G + K875T + N892YE2295 + T631N + A769D + L775A + D801G + K875T + N892Y 35 90 166 44E2295 + S578K + P752K + G753E + S754E + D801G + A912T 35 90 70 46E229N + S754E + A769D + L775A + D801G + K875T + N892Y 35 90 71 47E229N + N672D + P752K + G753E + A769D + L775A + D801G + 35 90 119 48K875T + N892Y + N991D E229N + P752K + G753E + A769D + L775A + D801G +K875T + N892Y 35 90 120 48 S100D + E2295 + K360R + S578K + T631N +P752R + G753E + 35 90 119 50 S754E + A769D + L775A + D801G + K875T +N892Y E2295 + K360R + S578K + T631N + P752R + G753E + S754E + 35 90 11951 A769D + L775A + D801G + K875T + N892Y + N991D E229N + T631N + N672D +A769D + L775A + D801G + K875T + N892Y 35 90 70 52 A58L + E2295 + K360R +S578K + N672D + G753E + S754E + 35 90 70 52 A769D + L775A + K875T +N892Y E2295 + K360R + S578K + N672D + G753E + S754E + A769D + 35 90 7056 L775A + D845E + K875T + N892Y Q109R + N672D + G753E + S754E + A769E +L775A + D801G + 35 90 71 56 K875T + N892Y E2295 + K360R + S578K +T631N + N672D + P752R + G753E + 35 90 119 57 S754E + A769D + L775A +D801G + K875T + N892Y E2295 + S578K + N672D + A769D + L775A + K875T +N892Y 35 90 120 58 L46D + E2295 + K360R + S578K + N672D + G753E +S754E + 35 90 70 58 A769D + L775A + A843P + K875T + N892Y E229N +S635E + T649K + I656V + N672D + A769D + L775A + 35 90 166 59 D801G +K875T + N892Y L46D + E2295 + K360R + S578K + T631N + N672D + G753E + 3590 70 59 S754E + A769D + L775A + K875T + N892Y E229S + S578K + P752K +G753E + S754E + A912T 35 90 166 61 Q109R + E2295 + G753E + S754E +A769T + L775A + D801G + 35 90 166 61 K875T + N892Y + A911V E2295 +K360R + S578K + P752R + G753E + S754E + A769D + 35 90 166 62 L775A +D801G + K875T + N892Y Q109R + E229S + S578K + P752R + G753E 35 90 166 62Q109R + E229S + A769T + L775A + D801G + K875T + N892Y 35 90 166 62L46D + E229S + S578K + G753E + S754E + A769D + L775A + 35 90 167 63D801G + K875T + N892Y Q109R + E229S + T631N + G753E + S754E + A769T +L775A + 35 90 166 63 D801G + K875T + N892Y Q109R + E229S + D458S +G753E + S754E + A769T + L775A + 35 90 166 64 D801G + K875T + N892YQ109R + E229S + G753E + S754E + A769T + L775A + D801G + 35 90 166 64K875T + N892Y S100D + E229S + S578K + G753E + A912T 35 90 166 64 E229S +K360R + S578K + P752K + G753E + S754E + A769D + 35 90 71 64 L775A +D801G + K875T + N892Y E229N + P752K + G753E + S754E + A769D + L775A +D801G + 35 90 166 64 K875T + N892Y E229N + S635E + T649K + I656V +N672D + G753E + A769D + 35 90 168 64 L775A + D801G + K875T + N892Y +N991D Q109R + E229S + S635T + G753E + S754E + A769T + L775A + 35 90 16665 D801G + K875T + N892Y E229N + S635E + T649K + I656V + N672D + S757D +A769D + 35 90 168 65 L775A + D801G + A843P + K875T + N892Y E229N +N672D + P752R + S754E + A769D + L775A + D801G + 35 90 70 66 K875T +N892Y E229N + K567R + S635E + T649K + I656V + N672D + G753E + 35 90 16866 A769D + L775A + D801G + K875T + N892Y E229S + S578K + G753E + D801G +A912T 35 90 166 66 Q109R + E229S + G753E + S754E + A769T + L775A +D801G + 35 90 166 66 K875T + N892Y + D901A Q109R + A190Q + E229S +G753E + S754E + A769T + L775A + 35 90 166 66 D801G + K875T + N892YQ109R + E229S + A769T + L775A + D801G + A843P + K875T + N892Y 35 90 16666 E229N + I234V + S635E + T649K + I656V + N672D + A769D + 35 90 120 66L775A + D801G + A843P + K875T + N892Y E229N + S635E + T649K + I656V +N672D + A769D + L775A + 35 90 168 66 D801G + A843P + K875T + N892YQ109R + E229S + G753E + S754E + A769T + L775A + D801G + 35 90 166 67K875T + N892Y + N1008D T631N + A769D + L775A + D801G + K875T + N892Y 3590 166 67 Q109R + E229S + A492L + A769T + L775A + D801G + K875T + N892Y35 90 166 67 E229N + P752K + G753E + A769D + L775A + D801G + A843P + 3590 168 68 K875T + N892Y E229S + S578N + N672D + A769D + L775A + D801G +K875T + N892Y 35 90 168 68 727\+ E229S + K360R + S578K + N672D + G753E +S754E + 35 90 166 69 A769D + L775A + D801G + K875T + N892Y E229S +P752K + G753E + A769D + L775A + D801G + K875T + N892Y 35 90 168 69E229N + S635E + T649K + I656V + N672D + S754E + A769D + 35 90 166 69L775A + D801G + K875T + N892Y L46D + E229S + I234V + K360R + S578K +N672D + G753E + 35 90 168 69 S754E + A769D + L775A + D801G + K875T +N892Y E229N + I234V + N672D + G753E + S754E + A769D + L775A + 35 90 16870 D801G + K875T + N892Y + N1008D L46D + E229S + K360R + S578K + N672D +G753E + S754E + 35 90 168 70 A769D + L775A + D801G + K875T + N892YE229N + N672D + P752K + A769D + L775A + D801G + K875T + N892Y 35 90 16670 Q109R + E229S + P752K + G753E + S754E + A769T + L775A + 35 90 166 70D801G + K875T + N892Y E229N + N672D + A769D + L775A + D801G + K875T +N892Y 35 90 70 70 Q109R + E229S + T631N + S635E + T649K + N672D +P752R + 35 90 167 71 G753E + S754E + A769T + L775A + D801G + K875T +N892Y Q109R + E229S + G753E + S754E + A769D + L775A + P779V + 35 90 16671 D801G + K875T + N892Y E229N + D458S + N672D + G753E + S754E + A769D +L775A + 35 90 166 72 D801G + K875T + N892Y L46D + E229S + K360R +S578K + N672D + G753E + S754E + 35 90 168 72 A769D + L775A + D801G +K875T + N892Y A58L + E229N + N672D + G753E + S754E + A769D + L775A + 3590 166 73 D801G + K875T + N892Y A159P + E229N + N672D + G753E + S754E +A769D + L775A + 35 90 166 73 D801G + K875T + N892Y E229N + S635E +T649K + I656V + N672D + G753E + S754E + 35 90 166 74 A769D + L775A +D801G + K875T + N892Y E229N + N672D + G753E + S754E + A769D + L775A +D801G + 35 90 168 75 K875T + N892Y + N1008D E229S + K360R + S578K +N672D + P752R + A769D + L775A + 35 90 71 75 D801G + K875T + N892YQ109R + A159P + E229S + S635E + G753E + S754E + A769D + 35 90 167 75L775A + D801G + K875T + N892Y E229S + S578K + N672D + G753E + S754E +A769D + L775A + 35 90 168 75 K875T + N892Y E229N + S635E + T649K +I656V + N672D + G753E + A769D + 35 90 168 76 L775A + D801G + K875T +N892Y + T923H E229S + T631N + P752K + G753E + A769D + L775A + D801G + 3590 167 76 K875T + N892Y E229N + S635E + T649K + I656V + N672D + P752R +G753E + 35 90 166 77 S754E + A769D + L775A + D801G + K875T + N892YE229N + S635E + T649K + I656V + N672D + P752K + G753E + 35 90 168 77A769D + L775A + D801G + A843P + K875T + N892Y A190Q + E229S + K360R +S578K + P752R + G753E + S754E + 35 90 168 77 A769D + L775A + D801G +K875T + N892Y A58L + E229S + I234V + N672D + G753E + S754E + A769D + 3590 168 78 L775A + D801G + K875T + N892Y L46D + E229S + K360R + S578K +N672D + G753E + S754E + 35 90 70 78 A769D + L775A + K875T + N892Y +A912T L46D + E229S + K360R + S578K + N672D + P752K + G753E + 35 90 70 78S754E + A769D + L775A + K875T + N892Y E229S + N672D + G753E + L775A +D801G + K875T + N892Y + 35 90 166 78 A911V A159P + E229N + N672D +G753E + S754E + A769D + L775A + 35 90 166 79 D801G + K875T + N892Y +N1008D E229N + S635E + N672D + G753E + S754E + A769D + L775A + 35 90 16880 D801G + K875T + N892Y + N1008D E229N + S635E + T649K + I656V +N672D + A769D + L775A + 35 90 120 80 D801G + A843P + K875T + N892Y +N991D E229N + T631N + N672D + G753E + S754E + A769D + L775A + 35 90 16680 D801G + K875T + N892Y E229N + N672D + P752K + G753E + S754E + A769D +L775A + 35 90 168 80 D801G + K875T + N892Y + N1008D E229N + N672D +G753E + S754E + A769D + L775A + D801G + 35 90 166 80 K875T + N892Y +V998K Q109R + E229S + K567R + A769T + L775A + D801G + K875T + N892Y 3590 166 81 E229N + S635T + N672D + G753E + S754E + A769D + L775A + 35 90166 82 D801G + K875T + N892Y Q109R + A159P + E229S + S635E + T649K +G753E + S754E + 35 90 167 82 A769D + L775A + D801G + K875T + N892Y +N1008D E229N + N672D + G753E + S754E + A769D + L775A + D801G + 35 90 16682 A843P + K875T + N892Y E229N + N672D + I703L + A769D + L775A + D801G +K875T + 35 90 166 82 N892Y E229S + K360R + S578K + T631N + P752R +G753E + S754E + 35 90 120 82 A769D + L775A + D801G + K875T + N892YE229N + N672D + I703L + G753E + S754E + A769D + L775A + 35 90 166 82D801G + K875T + N892Y E229S + S635E + T649K + I656V + N672D + A769D +L775A + 35 90 168 83 D801G + K875T + N892Y Q109R + E229S + N672D +P752R + G753E + S754E + A769S + 35 90 168 83 L775A + D801G + K875T +N892Y Q109R + A159P + E229S + L5331 + S582K + N672D + M728V + 35 90 16884 G753E + S754E + A769T + L775A + D801G + A843P + K875T + N892Y Q109R +A159P + E229S + N672D + G753E + S754E + A769T + 35 90 168 84 L775A +D801G + K875T + N892Y + A911V E229N + S635E + T649K + I656V + N672D +P752K + G753E + 35 90 166 84 A769D + L775A + D801G + K875T + N892YQ109R + A159P + E229S + G753E + S754E + A769T + L775A + 35 90 166 85D801G + K875T + N892Y E229N + S635E + T649K + I656V + N672D + G753E +A769D + 35 90 168 86 L775A + D801G + K875T + N892Y E229S + K567R +P752K + G753E + A769D + L775A + D801G + 35 90 167 86 K875T + N892YE229N + N672D + G753E + A769D + L775A + D801G + K875T + N892Y 35 90 16687 Q109R + E229S + T631N + G753E + S754E + A769D + L775A + 35 90 166 87D801G + K875T + N892Y L46D + E229S + K360R + S578K + A624E + N672D +G753E + 35 90 168 87 S754E + A769D + L775A + D801G + K875T + N892YQ109R + A159P + E229S + S635E + T649K + G753E + S754E + 35 90 167 87A769D + L775A + D801G + K875T + N892Y + A912T E229N + T631N + S635E +T649K + I656V + N672D + A769D + 35 90 70 88 L775A + D801G + K875T +N892Y Q109R + E229S + K451R + N672D + G753E + S754E + A769T + 35 90 16688 L775A + D801G + K875T + N892Y E229N + S635E + T649K + I656V + N672D +P752K + G753E + 35 90 166 89 S754E + A769D + L775A + D801G + K875T +N892Y S100D + Q109R + A159P + E229S + S635E + T649K + G753E + 35 90 16789 S754E + A769D + L775A + D801G + K875T + N892Y E229N + S635E + T649K +I656V + N672D + P752R + G753E + 35 90 168 89 A769D + L775A + D801G +A843P + K875T + N892Y Q109R + E2295 + N672D + P752R + G753E + S754E +A769T + 35 90 168 90 L775A + D801G + K875T + N892Y + A911V E229N +K567R + S635E + T649K + I656V + N672D + A769D + 35 90 70 90 L775A +D801G + K875T + N892Y Q109R + A159P + E229S + N672D + G753E + S754E +A769T + 35 90 168 90 L775A + D801G + K875T + N892Y E229N + N672D +P752R + G753E + S754E + A769D + L775A + 35 90 166 90 D801G + K875T +N892Y E229S + S635E + T649K + I656V + N672D + G753E + S754E + 35 90 14990 A769D + L775A + V800P + D801G + K875T + N892Y Q109R + E229S + S635E +T649K + G753E + S754E + A769D + 35 90 166 91 L775A + D801G + K875T +N892Y Q109R + A159P + E229S + G753E + S754E + A769D + L775A + 35 90 16891 D801G + K875T + N892Y Q109R + E229S + N672D + P752R + G753E + S754E +A769T + 35 90 168 91 L775A + D801G + K875T + N892Y + A912T Q109R +A190Q + E229S + G753E + S754E + A769D + L775A + 35 90 166 91 D801G +K875T + N892Y Q109R + A159P + A190Q + E229S + G753E + S754E + A769D + 3590 168 92 L775A + D801G + K875T + N892Y E229S + P752K + G753E + A769D +L775A + D801G + A843P + 35 90 168 92 K875T + N892Y Q109R + A159P +E229S + S635E + T649K + P752R + G753E + 35 90 167 92 S754E + A769D +L775A + D801G + K875T + N892Y E229N + N672D + G753E + S754E + A769D +L775A + D801G + 35 90 168 93 K875T + N892Y + N1008D Q109R + E229S +S635T + G753E + S754E + A769D + L775A + 35 90 166 95 D801G + K875T +N892Y Q109R + A159P + E229S + D458S + G753E + S754E + A769D + 35 90 16895 L775A + D801G + K875T + N892Y E229N + N672D + P752K + G753E + A769D +L775A + D801G + 35 90 168 96 A843P + K875T + N892Y E229N + N672D +P752R + G753E + A769D + L775A + D801G + 35 90 166 96 K875T + N892YE229N + N672D + G753E + S754E + A769D + L775A + D801G + 35 90 166 97K875T + N892Y E229S + S582K + S635E + T649K + I656V + N672D + G738L + 3590 168 97 G753E + S754E + A769D + L775A + D801G + K875T + N892Y Q109R +A159P + E229S + S635E + T649K + N672D + P752K + 35 90 167 97 G753E +S754E + A769T + L775A + D801G + K875T + N892Y Q109R + E229S + T631N +N672D + P752K + G753E + S754E + 35 90 167 98 A769T + L775A + D801G +K875T + N892Y E229N + T631N + N672D + P752K + G753E + A769D + L775A + 3590 167 98 D801G + K875T + N892Y E229N + S635E + T649K + I656V + N672D +P752R + G753E + 35 90 167 100 S754E + A769D + L775A + D801G + A843P +K875T + N892Y E229N + T631N + N672D + G753E + S754E + A769D + L775A + 3590 168 100 D801G + K875T + N892Y + N1008D E229N + N672D + P752K +G753E + A769D + L775A + D801G + 35 90 167 101 K875T + N892Y + T923HQ89Y + Q109R + A159P + E229S + S635E + T649K + N672D + 35 90 167 101G753E + S754E + A769T + L775A + D801G + K875T + N892Y A190Q + E229S +S635E + T649K + 1656V + N672D + I703L + 35 90 168 102 G753E + S754E +A769D + L775A + D801G + K875T + N892Y + N1008D Q109R + E229S + N672D +P752R + G753E + S754E + A769T + 35 90 168 103 L775A + D801G + K875T +N892Y Q109R + E229S + N672D + G753E + S754E + A769T + L775A + 35 90 166103 D801G + K875T + N892Y Q109R + E229S + G753E + S754E + A769D +L775A + D801G + 35 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S754E + 35 90 168 188A769D + L775A + D801G + A843P + K875T + N892Y Q109R + E229S + T631N +N672D + P752R + G753E + S754E + 35 90 149 188 S757D + A769D + L775A +D801G + K875T + N892Y Q109R + A159P + E229S + S635E + T649K + N672D +I703L + 35 90 149 188 G753E + S754E + S757D + A769T + L775A + D801G +K875T + N892Y Q89Y + E229S + N672D + G753E + S754E + A769D + L775A + 3590 168 188 D801G + K875T + N892Y E229S + S635E + T649K + I656V + N672D +P752K + G753E + 35 90 168 188 A769D + L775A + D801G + A843P + K875T +N892Y E229S + A492L + S635E + T649K + I656V + N672D + G753E + 35 90 168189 A769D + L775A + D801G + K875T + N892Y E229S + D458S + S582K +A624E + N672D + G753E + S754E + 35 90 168 189 A769D + L775A + D801G +K875T + N892Y E229S + D458S + T631N + S635E + N672D + G753E + S754E + 3590 168 189 A769D + L775A + D801G + K875T + N892Y E229S + S635E + T649K +I656V + N672D + P752K + G753E + 35 90 168 193 A769D + L775A + V800P +D801G + K875T + N892Y + N1008D E229S + D458S + N672D + G753E + S754E +A769D + L775A + 35 90 149 193 D801G + K875T + N892Y + N1008D T18D +E229S + N440K + N672D + G753E + S754E + A769D + 35 90 168 194 L775A +D801G + K875T + N892Y A159P + E229S + N440K + N672D + G753E + S754E +A769D + 35 90 168 196 L775A + D801G + K875T + N892Y Q109R + A159P +E229S + S635E + T649K + N672D + P752R + 35 90 168 197 G753E + S754E +A769D + L775A + D801G + K875T + N892Y E229S + I234V + S635E + T649K +I656V + N672D + G753E + 35 90 168 197 A769D + L775A + D801G + K875T +N892Y Q109R + E229S + T631N + N672D + P752R + G753E + S754E + 35 90 149197 A769D + L775A + D801G + K875T + N892Y + N1008D E229S + D458S +T631E + N672D + G753E + S754E + A769D + 35 90 168 200 L775A + D801G +K875T + N892Y A190Q + E229S + S635E + T649K + 1656V + N672D + I703L + 3590 168 200 G753E + S754E + A769D + L775A + V800P + D801G + K875T +N892Y + N1008D E229S + D458S + N672D + P752R + G753E + S754E + A769D +35 90 168 200 L775A + D801G + K875T + N892Y A190Q + E229S + T631E +S635E + T649K + 1656V + N672D + 35 90 168 203 I703L + G753E + S754E +A769D + L775A + D801G + K875T + N892Y + N1008D E229S + N440K + A492L +S582K + A624E + N672D + G753E + 35 90 168 203 S754E + A769D + L775A +D801G + K875T + N892Y E229S + D458S + K567R + T631N + N672D + G753E +S754E + 35 90 168 203 A769D + L775A + D801G + K875T + N892Y L46D +E229S + K360R + S578K + N672D + G753E + S754E + 35 90 168 203 A769D +L775A + D801G + K875T + N892Y E229S + S582K + S635E + T649K + 1656V +N672D + I703L + 35 90 168 204 G753E + S754E + A769D + L775A + D801G +K875T + N892Y + N1008D Q109R + A159P + E229S + S635Q + T649K + G753E +S754E + 35 90 167 204 S757D + A769D + L775A + D801G + K875T + N892YT18D + E229S + S635E + T649K + I656V + N672D + G753E + 35 90 149 205S754E + A769D + L775A + D801G + K875T + N892Y Q109R + A159P + E229S +G753E + S754E + S757D + A769D + 35 90 168 206 L775A + D801G + K875T +N892Y E229S + N672D + G753E + S754E + A769D + L775A + D801G + 35 90 168206 K875T + N892Y E229N + T631N + N672D + P752K + G753E + A769D +L775A + 35 90 149 207 D801G + K875T + N892Y + A911V Q109R + E229S +T631N + S635E + T649K + N672D + P752R + 35 90 149 209 G753E + S754E +A769D + L775A + D801G + K875T + N892Y E229S + D458S + K567R + N672D +M728V + G753E + S754E + 35 90 168 209 A769D + L775A + D801G + K875T +N892Y E229S + I234V + S582K + N672D + G753E + S754E + A769D + 35 90 168212 L775A + D801G + K875T + N892Y E229S + N440K + S582K + T631N +N672D + G753E + S754E + 35 90 168 213 A769D + L775A + D801G + K875T +N892Y + N1008D A190Q + E229S + I234V + S582K + N672D + G753E + S754E +35 90 168 215 A769D + L775A + D801G + K875T + N892Y A190Q + E229S +I234V + A624E + N672D + G753E + S754E + 35 90 168 216 A769D + L775A +D801G + K875T + N892Y E229S + N440K + S582K + A624E + S635E + N672D +G753E + 35 90 168 216 S754E + A769D + L775A + D801G + K875T + N892YE229S + S635E + T649K + I656V + N672D + G753E + S754E + 35 90 168 218A769D + L775A + D801G + K875T + N892Y S100D + E229S + S635E + T649K +1656V + N672D + I703L + 35 90 168 219 G753E + A769D + L775A + D801G +A843P + K875T + N892Y + N1008D A190Q + E2295 + S635E + T649K + 1656V +N672D + I703L + 35 90 168 224 P752R + G753E + S754E + A769D + L775A +D801G + K875T + N892Y + N1008D Q89Y + E2295 + N440K + 5582K + A624E +N672D + G753E + 35 90 168 225 S754E + A769D + L775A + D801G + K875T +N892Y A190Q + E2295 + S635E + T649K + 1656V + N672D + I703L + 35 90 168231 G753E + S754E + 5757D + A769D + L775A + D801G + K875T + N892Y +N1008D E2295 + I234V + N672D + G753E + S754E + A769D + L775A + 35 90 149235 D801G + A843P + K875T + N892Y E2295 + S635E + T649K + I656V +N672D + G753E + S754E + 35 90 168 236 S757D + A769D + L775A + D801G +K875T + N892Y T18D + E229S + S582K + N672D + G753E + S754E + A769D + 3590 168 239 L775A + D801G + K875T + N892Y E2295 + D4585 + T631N + N672D +G753E + S754E + S757D + 35 90 168 239 A769D + L775A + D801G + K875T +N892Y E2295 + S635E + T649K + I656V + N672D + P752R + G753E + 35 90 168239 S754E + A769D + L775A + D801G + K875T + N892Y + N1008D E2295 +N440K + 5582K + A624E + N672D + G753E + S754E + 35 90 168 242 A769D +L775A + V800P + D801G + K875T + N892Y A190Q + E2295 + D4585 + T631N +N672D + G753E + S754E + 35 90 168 245 A769D + L775A + D801G + K875T +N892Y E2295 + K360G + D4585 + S582K + N672D + G753E + S754E + 35 90 168251 A769D + L775A + D801G + K875T + N892Y + N1008D S100D + E2295 +S635E + T649K + 1656V + N672D + G753E + 35 90 149 253 S754E + A769D +L775A + D801G + K875T + N892Y A190Q + E229S + S635E + T649K + 1656V +N672D + G753E + 35 90 149 261 S754E + A769D + L775A + D801G + K875T +N892Y S100D + A190Q + E229S + S635E + T649K + 1656V + N672D + 35 90 168263 I703L + G753E + S754E + A769D + L775A + D801G + K875T + N892Y +N1008D E229S + D458S + K567R + S635E + N672D + G753E + S754E + 35 90 168266 A769D + L775A + D801G + K875T + N892Y E229S + D458S + N672D +G753E + S754E + A769D + L775A + 35 90 149 281 D801G + A843P + K875T +N892Y E229S + T631N + N672D + I703L + P752K + G753E + A769D + 35 90 149284 L775A + D801G + K875T + N892Y E229S + N440K + S582K + N672D +G753E + S754E + A769D + 35 90 149 296 L775A + D801G + A843P + K875T +N892Y + N1008D

TABLE 32 Half-life of purified variants: Temperature (T) 35° C.,detergent concentration 95% Incubation Half- T Detergent time lifeMutations relative to SEQ ID NO: 6 (° C.) (%) (h) (h) No mutations(Wild-type) 35 95 1 <0.2 E229S + K360G + D458S + S582K + T631N + S635E +N672D + 35 95 168 40 I703L + M728V + G753E + S754E + S757D + A769D +L775A + D801G + K875T + N892Y E229S + D458S + S582K + T631N + S635E +N672D + M728V + 35 95 168 27 G753E + S754E + S757D + A769D + L775A +D777R + V800P + D801G + K875T + N892Y E229S + D458S + S582K + T631N +S635E + N672D + M728V + 35 95 168 39 G753E + S754E + S757D + A769D +L775A + D801G + A843P + K875T + N892Y E229S + D458S + S582K + T631N +S635E + N672D + M728V + 35 95 168 46 G753E + S754E + S757D + A769D +L775A + D801G + A843P + K875T + N892Y E229S + D458S + S582K + T631N +S635E + T664K + N672D + 35 95 168 41 M728V + G753E + S754E + S757D +A769D + L775A + D801G + K875T + N892Y E229S + V352I + D458S + S582K +T631N + S635E + N672D + 35 95 168 43 M728V + G753E + S754E + S757D +A769D + L775A + D801G + K875T + N892Y + K1016T S100D + E229S + K360G +A624E + S635E + T649K + N672D + 35 95 168 54 G753E + S754E + A769D +L775A + D777K + D801G + K875T + N892Y + K1016T S100D + E2295 + V352I +K360G + D458S + A624E + S635E + 35 95 168 42 T649K + I656V + N672D +G753E + S754R + S757D + A769D + L775A + D777K + D801G + K875T + N892Y +K1016T E2295 + A624E + S635E + T649K + I656V + N672D + L748T + 35 95 16837 G753E + S754R + S757D + A769D + L775A + D777K + D801G + K875T + N892YE2295 + S582K + A624E + S635E + T649K + I656V + N672D + 35 95 168 38G753E + S754R + S757D + A769D + L775A + D777K + V800P + D801G + K875T +N892Y E2295 + A624E + S635E + T649K + N672D + G753E + S754E + 35 95 16845 A769D + L775A + D777K + D801G + K875T + N892Y E2295 + A624E + S635E +T649K + I656V + N672D + G753E + 35 95 168 55 S754R + S757D + A769D +L775A + D777R + D801G + A843P + K875T + N892Y E2295 + D4585 + K567R +S582K + S635E + N672D + M728V + 35 95 168 23 G753E + S754R + S757D +A769D + L775A + D777K + D801G + K875T + N892Y + K1016T E229S + D458S +K567R + S582K + S635E + N672D + G753E + 35 95 168 26 S754E + A769D +L775A + D777R + V800P + D801G + K875T + N892Y E229S + D458S + K567R +S582K + S635E + N672D + G753E + 35 95 168 29 S754E + A769D + L775A +D777R + D801G + K875T + N892Y + N1008D E229S + D458S + K567R + S582K +S635E + T649K + N672D + 35 95 168 29 G753E + S754E + A769D + L775A +D777K + D801G + K875T + N892Y E229S + N399K + D458S + A492H + K567R +S582K + S635E + 35 95 168 61 T649K + N672D + G753E + S754E + A769D +L775A + D777R + D801G + K875T + N892Y E229S + D458S + K567R + S582K +S635E + N672D + G753E + 35 95 168 23 S754E + S757D + A769D + L775A +D777R + D801G + K875T + N892Y E229S + D458S + K567R + S582K + S635E +T664K + N672D + 35 95 168 25 G753E + S754E + A769D + L775A + D777R +K792Y + D801G + K875T + N892Y E229S + D458S + K567R + S582K + S635E +T664K + N672D + 35 95 168 25 G753E + S754E + A769D + L775A + D777R +D801G + K875T + N892Y E229S + D458S + K567R + S582K + S635E + N672D +M728V + 35 95 168 30 G753E + S754E + A769D + L775A + D777R + D801G +K875T + N892Y S100D + E229S + K360G + D458S + S582K + A624E + N672D + 3595 168 60 G753E + S754E + S757D + A769D + L775A + D801G + A843P +K875T + N892Y + N1008D S100D + E229S + K360G + D458S + S582K + N672D +G753E + 35 95 168 50 S754E + S757D + A769D + L775A + D801G + A843P +K875T + N892Y + N1008D S100D + E229S + K360G + D458S + S582K + S635E +N672D + 35 95 168 51 G753E + S754E + 5757D + A769D + L775A + V800P +D801G + A843P + K875T + N892Y + N1008D S100D + E229S + K360G + D458S +S582K + S635E + T649K + 35 95 168 59 N672D + G753E + S754E + S757D +A769D + L775A + D801G + A843P + K875T + N892Y + N1008D E2295 + S635E +T649R + N672D + P752R + G753E + S754E + 35 95 168 37 A769D + L775A +D777K + D801G + K875T + N892Y E2295 + N672D + P752R + G753E + S754E +S757D + A769D + 35 95 168 47 L775A + D801G + A843P + K875T + N892YE2295 + S635E + T649K + I656V + N672D + G753E + A769D + 35 95 168 24L775A + D801G + K875T + N892Y S100D + E2295 + K360G + D4585 + S582K +N672D + G753E + 35 95 168 45 S754E + A769D + L775A + D801G + K875T +N892Y + N1008D A190Q + E2295 + K360G + D4585 + S582K + N672D + G753E +35 95 168 41 S754E + A769D + L775A + D801G + K875T + N892Y + N1008D

TABLE 33 Half-life of purified variants: Temperature (T) 37° C.,detergent concentration 90% T Detergent Incubation Half- Mutationsrelative to SEQ ID NO: 6 (° C.) (%) time (h) life (h) No mutations(Wild-type) 37 90 1 <0.2 Q109R + A159P + E229S + V352I + S635E + 37 90120 29 T649K + N672D + I703L + G753E + S754E + S757D + A769T + L775A +D801G + K875T + N892Y E229N + T631N + N672D + G753E + S754E + 37 90 12040 A769D + L775A + D801G + K875T + N892Y + N1008D E229S + N440K +S582K + A624E + S635E + 37 90 120 53 N672D + G753E + S754E + S757D +A769D + L775A + D801G + A843P + K875T + N892Y E229S + I234V + T631N +N672D + I703L + 37 90 120 63 P752K + G753E + A769D + L775A + D801G +K875T + N892Y Q109R + A159P + E229S + S582K + S635Q + 37 90 120 64T649K + G753E + S754E + S757D + A769D + L775A + D801G + K875T + N892YQ109R + A159P + A190Q + E229S + G753E + 37 90 120 65 S754E + S757D +A769D + L775A + D801G + K875T + N892Y A159P + E229S + S635E + T649K +I656V + 37 90 120 67 N672D + G753E + S754E + A769D + L775A + V800P +D801G + K875T + N892Y T18D + Q89Y + E229S + S635E + T649K + 37 90 120 69I656V + N672D + G753E + S754E + A769D + L775A + D801G + K875T + N892YT18D + A190Q + E229S + S635E + T649K + 37 90 120 70 I656V + N672D +G753E + S754E + A769D + L775A + D801G + K875T + N892Y Q109R + A159P +E229S + T631N + G753E + 37 90 120 71 S754E + S757D + A769D + L775A +D801G + K875T + N892Y A58L + E229S + N440K + S582K + T631N + 37 90 12071 N672D + G753E + S754E + A769D + L775A + D801G + K875T + N892Y +N1008D E229S + S635E + T649K + I656V + N672D + 37 90 120 72 G753E +S754E + A769D + L775A + V800P + D801G + K875T + N892Y E229S + T631N +N672D + I703L + P752R + 37 90 120 73 G753E + A769D + L775A + D801G +K875T + N892Y T18D + Q109R + E229S + T631N + S635E + 37 90 120 74T649K + N672D + P752R + G753E + S754E + A769D + L775A + D801G + K875T +N892Y A190Q + E229S + D458S + K567R + S635E + 37 90 120 74 N672D +G753E + S754E + A769D + L775A + D801G + K875T + N892Y Q109R + A159P +E229S + T631E + S635Q + 37 90 120 77 T649K + G753E + S754E + S757D +A769D + L775A + D801G + K875T + N892Y E229S + T631N + N672D + I703L +P752K + 37 90 120 77 G753E + A769D + L775A + D801G + K875T + N892YE229S + T631N + N672D + I703L + P752K + 37 90 120 79 G753E + A769D +L775A + D801G + K875T + N892Y + N1008D Q89Y + E229S + D458S + T631N +N672D + 37 90 120 80 G753E + S754E + S757D + A769D + L775A + D801G +K875T + N892Y A190Q + E229S + D458S + T631N + N672D + 37 90 120 80G753E + S754E + A769D + L775A + D801G + K875T + N892Y Q109R + E229S +T631N + N672D + M728V + 37 90 120 82 P752R + G753E + S754E + S757D +A769D + L775A + D801G + K875T + N892Y Q109R + A159P + E229S + T631N +S635Q + 37 90 120 82 T649K + G753E + S754E + S757D + A769D + L775A +D801G + K875T + N892Y Q109R + A159P + E229S + S635Q + T649K + 37 90 12082 G753E + S754E + S757D + A769D + L775A + D801G + K875T + N892Y Q109R +A159P + E229S + S635E + T649K + 37 90 120 83 N672D + I703L + M728V +G753E + S754E + S757D + A769T + L775A + D801G + K875T + N892Y E229S +D458S + T631N + N672D + G753E + 37 90 120 83 S754E + S757D + A769D +L775A + D801G + K875T + N892Y E229S + D458S + S582K + T631N + N672D + 3790 120 84 M728V + G753E + S754E + S757D + A769D + L775A + D801G +K875T + N892Y Q109R + E229S + S582K + T631N + S635E + 37 90 120 84T649K + N672D + P752R + G753E + S754E + A769D + L775A + D801G + K875T +N892Y A190Q + E229S + D458S + T631N + S635E + 37 90 120 84 N672D +G753E + S754E + A769D + L775A + D801G + K875T + N892Y E229S + T631N +N672D + I703L + P752R + 37 90 120 84 G753E + S754E + A769D + L775A +D801G + K875T + N892Y E229S + S635E + T649K + I656V + N672D + 37 90 12084 P752K + G753E + S754E + A769D + L775A + V800P + D801G + K875T + N892YL46D + E229S + T631N + N672D + I703L + 37 90 120 84 P752K + G753E +A769D + L775A + D801G + K875T + N892Y A190Q + E229S + R284G + S635E +T649K + 37 90 120 85 I656V + N672D + G753E + S754E + A769D + L775A +V800P + D801G + K875T + N892Y T18D + A190Q + E229S + D458S + T631N + 3790 120 85 N672D + G753E + S754E + A769D + L775A + D801G + K875T + N892YE229S + S635E + T649K + I656V + N672D + 37 90 120 86 G753E + S754E +A769D + L775A + V800P + D801G + A843P + K875T + N892Y E229S + I234V +N440K + S582K + A624E + 37 90 120 86 S635E + N672D + G753E + S754E +A769D + L775A + D801G + K875T + N892Y Q109R + A159P + E229S + S635E +T649K + 37 90 120 86 N672D + I703L + G753E + S754E + S757D + A769T +L775A + V800P + D801G + K875T + N892Y Q109R + E229S + K360G + T631N +S635E + 37 90 120 87 T649K + N672D + P752R + G753E + S754E + A769D +L775A + D801G + K875T + N892Y E229S + S582K + T631N + N672D + I703L + 3790 120 87 P752K + G753E + A769D + L775A + D801G + K875T + N892Y E229S +I234V + S582K + N672D + M728V + 37 90 120 87 G753E + S754E + A769D +L775A + D801G + K875T + N892Y E229S + A492L + S635E + T649K + I656V + 3790 120 87 N672D + G753E + S754E + A769D + L775A + V800P + D801G +K875T + N892Y E229S + N440K + S582K + A624E + S635E + 37 90 120 87N672D + G753E + S754E + A769D + L775A + D777K + D801G + K875T + N892YL46D + E229S + N440K + S582K + T631N + 37 90 120 88 N672D + G753E +S754E + A769D + L775A + D801G + K875T + N892Y + N1008D Q109R + A159P +E229S + A624E + S635E + 37 90 120 88 T649K + N672D + I703L + G753E +S754E + S757D + A769T + L775A + D801G + K875T + N892Y E229S + N672D +G753E + S754E + A769D + 37 90 120 88 L775A + D801G + K875T + N892YE229S + A492L + S635E + T649K + I656V + 37 90 120 88 N672D + G753E +A769D + L775A + D801G + K875T + N892Y + N1008D Q109R + A159P + E229S +T631N + S635E + 37 90 120 88 T649K + N672D + I703L + G753E + S754E +S757D + A769T + L775A + D801G + K875T + N892Y T18D + S100D + E229S +S635E + T649K + 37 90 120 88 I656V + N672D + I703L + G753E + A769D +L775A + D801G + A843P + K875T + N892Y + N1008D T18D + E229S + S582K +T664K + N672D + 37 90 120 89 G753E + S754E + A769D + L775A + D801G +K875T + N892Y Q109R + A159P + E229S + T631N + S635E + 37 90 120 89T649K + N672D + P752R + G753E + S754E + A769D + L775A + D801G + K875T +N892Y A58L + E229S + S635E + T649K + I656V + 37 90 120 91 N672D +G753E + S754E + S757D + A769D + L775A + D801G + K875T + N892Y E229S +V352I + D458S + K567R + S635E + 37 90 120 92 N672D + G753E + S754E 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G753E + S754R + S757D + A769D + L775A + D777K + D801G + K875T +N892Y A190Q + E229S + K360G + D458S + S582K + 37 90 120 141 N672D +G753E + S754E + A769D + L775A + D801G + K875T + N892Y + N1008D S100D +E229S + N440K + S582K + T631N + 37 90 120 142 N672D + G753E + S754E +A769D + L775A + D801G + K875T + N892Y + N1008D S100D + E229S + K360G +D458S + S582K + 37 90 120 144 N672D + G753E + S754E + A769D + L775A +D801G + A843P + K875T + N892Y + N1008D E229S + I234V + A492L + N672D +G753E + 37 90 120 145 S754E + A769D + L775A + D777K + D801G + K875T +N892Y A190Q + E229S + K360G + D458S + S582K + 37 90 120 146 T664K +N672D + G753E + S754E + A769D + L775A + D801G + K875T + N892Y + N1008DA190Q + E229S + D458S + T631N + N672D + 37 90 120 147 G753E + S754E +A769D + L775A + D801G + A843P + K875T + N892Y E229S + N440K + S582K +S635E + N672D + 37 90 120 147 G753E + S754E + A769D + L775A + D801G +A843P + K875T + N892Y + N1008D E229S + A492L + S635E + T649K + I656V +37 90 120 147 N672D + P752R + G753E + S754E + A769D + L775A + D801G +K875T + N892Y + N1008D T18D + E229S + S582K + N672D + G753E + 37 90 120149 S754E + A769D + L77 5A + D801G + K875T + N892Y + T902F E229S +D458S + S582K + A624E + T631N + 37 90 120 149 N672D + M728V + G753E +S754E + S757D + A769D + L775A + D801G + K875T + N892Y E229S + D458S +T631E + N672D + G753E + 37 90 120 151 S754E + S757D + A769D + L775A +D801G + K875T + N892Y T18D + E229S + N440K + S582K + A624E + 37 90 120152 S635E + N672D + G753E + S754E + S757D + A769D + L775A + D801G +K875T + N892Y E229S + N440K + S582K + A624E + N672D + 37 90 120 152P752R + G753E + S754E + A769D + L775A + D801G + K875T + N892Y + N1008DE229S + N440K + S582K + A624E + T631N + 37 90 120 153 S635E + N672D +G753E + S754E + S757D + A769D + L775A + D801G + K875T + N892Y E229S +N440K + S582K + A624E + S635E + 37 90 120 154 N672D + G738L + G753E +S754E + S757D + A769D + L775A + D801G + K875T + N892Y E229S + I234V +A492L + S582K + N672D + 37 90 120 157 M728V + G753E + S754E + A769D +L775A + D801G + K875T + N892Y E229S + D458S + A492L + T631N + N672D + 3790 120 158 G753E + S754E + S757D + A769D + L775A + D801G + K875T + N892YA190Q + E229S + I234V + S582K + N672D + 37 90 120 158 G753E + S754E +S757D + A769D + L775A + D801G + K875T + N892Y E229S + N440K + S582K +N672D + P752R + 37 90 120 158 G753E + S754E + S757D + A769D + L775A +D801G + K875T + N892Y + N1008D E229S + S635E + T649K + I656V + N672D +37 90 120 159 G753E + S754R + S757D + A769D + L775A + D801G + A843P +K875T + N892Y A190Q + E229S + N440K + S582K + A624E + 37 90 120 162S635E + N672D + G753E + S754E + S757D + A769D + L775A + D801G + K875T +N892Y S100D + E229S + D458S + K567R + S582K + 37 90 120 163 S635E +N672D + G753E + S754E + A769D + L775A + D801G + K875T + N892Y E229S +S635E + N672D + P752R + G753E + 37 90 120 169 S754E + A769D + L775A +D777K + D801G + K875T + N892Y S100D + A190Q + E229S + K360G + D458S + 3790 120 170 S582K + N672D + G753E + S754E + A769D + L775A + D801G +K875T + N892Y + N1008D S100D + E229S + K360G + D458S + S582K + 37 90 120171 N672D + G753E + S754E + A769D + L775A + D801G + K875T + N892Y +N1008D E229S + D458S + S582K + T631N + S635E + 37 90 120 172 N672D +M728V + G753E + S754E + S757D + A769D + L775A + D801G + K875T + N892YS100D + E229S + K360G + D458S + S582K + 37 90 120 174 N672D + G753E +S754E + S757D + A769D + L775A + D801G + A843P + K875T + N892Y + N1008DE229S + I234V + A492L + S582K + N672D + 37 90 120 174 G753E + S754E +A769D + L775A + D801G + K875T + N892Y E229S + D458S + S582K + T631E +N672D + 37 90 120 175 G753E + S754E + A769D + L775A + D801G + K875T +N892Y E229S + I234V + A492L + N672D + G753E + 37 90 120 176 S754E +A769D + L775A + D777R + D801G + K875T + N892Y S100D + E229S + K360G +D458S + S582K + 37 90 120 179 N672D + G753E + S754E + S757D + A769D +L775A + D801G + A843P + K875T + N892Y + N1008D E229S + A624E + S635E +T649K + I656V + 37 90 120 185 N672D + G753E + S754R + S757D + A769D +L775A + D777K + D801G + K875T + N892Y S100D + E229S + K360G + D458S +S582K + 37 90 120 189 N672D + G753E + S754E + S757D + A769D + L775A +D801G + A843P + K875T + N892Y + T915A + N1008D T18D + E229S + D458S +T631N + N672D + 37 90 120 191 M728V + G753E + S754E + S757D + A769D +L775A + D801G + K875T + N892Y S100D + E229S + N440K + S582K + N672D + 3790 120 192 P752R + G753E + S754E + A769D + L775A + D801G + K875T +N892Y + N1008D T18D + E229S + S582K + N672D + G753E + 37 90 120 202S754E + P764K + A769D + L775A + D801G + K875T + N892Y E229S + D458S +T631E + N672D + G753E + 37 90 120 203 S754E + A769D + L775A + D777K +D801G + K875T + N892Y E229S + S582K + S635E + T649K + I656V + 37 90 120209 N672D + P752K + G753E + S757D + A769D + L775A + D801G + A843P +K875T + N892Y E229S + A624E + S635E + T649K + I656V + 37 90 120 210N672D + G738L + G753E + S754R + S757D + A769D + L775A + D777K + D801G +K875T + N892Y A159P + E229S + D458S + T631N + N672D + 37 90 120 223M728V + G753E + S754E + S757D + A769D + L775A + D801G + K875T + N892YA190Q + E229S + S582K + N672D + G753E + 37 90 120 226 S754E + A769D +L775A + D801G + K875T + N892Y A190Q + E229S + K360G + D458S + S582K + 3790 120 234 N672D + G753E + S754E + S757D + A769D + L775A + D801G +K875T + N892Y + N1008D

Example 10 Mini-TOM Wash of Xanthan Lyase and Endoglucanase Wildtypesand Variants

To assess improved detergent stability of xanthan lyase (XL) variantsand endoglucanase (EG) variants over their respective wildtypes (XL andEG wildtypes), the XL and EG wildtypes as well as combinations of XL andEG variants were incubated in heavy duty liquid detergent (PersilUniversal gel, PUG) for 13 days at 30° C. after which they weresubjected to a wash test in a mini-TOM (mini-terg-O-tometer) wash setup.The wash performance of the enzyme combinations stored 13 days in liquiddetergent were compared to an identical mini-TOM wash trial in which thedetergent and enzymes had not been stored prior to the wash (i.e. freshwash trial), but were added directly to the wash just before startingthe wash.

In brief, mini-TOM wash is a down-scaled version of TOM wash(terg-O-tometer wash, see WO2016/203064 for reference to the method) inwhich each beaker is 300 mL in size (instead of 2 L) and is filled with100 mL wash liqour (instead of 1 L). All other parameters (e.g.agitation) are identical to standard TOM wash.

Storage of enzymes in detergent: XL enzymes were diluted to 227 ppm andEG enzymes were diluted to 454 ppm in MQ water. Glass vials with lidswere prepared with 0.90 g of PUG detergent. 50 μL of diluted XL enzymeand 50 μL of diluted EG enzyme (wildtypes or variants) were added to thesame glass vial containing 0.90 g of PUG detergent. The glass vials werestored 13 days in a heating cabinet at 30° C.

Dosing in wash (stored samples): For each mini-TOM beaker 0.44 g storedPUG detergent with enzymes were diluted in 100 mL 16° dH water hardnesssolution (Ca:Mg 5:1, HCO₃×1.5) to give a final concentration of 0.05 ppmXL and 0.1 ppm EG.

Dosing in wash (fresh samples): For each mini-TOM beaker 0.44 g PUGdetergent were diluted in 100 mL 16 ° dH water hardness solution (Ca:Mg5:1, HCO₃×1.5) and added diluted XL and EG enzymes (wildtypes orvariants) directly to each mini-TOM beaker to give a final concentrationof 0.05 ppm XL and 0.1 ppm EG. Reference samples (i.e. detergent withoutXL and EG enzymes) were included in the wash trials to assess thedetergent cleaning level without XL and EG enzyme (reference).

Mini-TOM wash (stored or fresh samples): Each mini-TOM beaker containing100 mL of the PUG detergent wash liquor (4.4 g PUG/L wash liqour) withenzymes (stored or fresh) and 2 pieces of each of the commerciallyavailable stains C-S-05S (Mayonnaise with carbon black onCotton/soot—see Table 1 for results), C-S-17 (Fluid Make up onCotton—see Table 3 for results) and C-S-44 (Chocolate drink pure onCotton—see Table 2 for results) (Center for Testmaterials b.v.,Netherlands) cut into 2 cm0 circular stains was washed at 40° C. for 30min at 120 rpm, then rinsed in cold tap water for 30 seconds, thenplaced on a sheet of filter paper to dry overnight at room temperatureand then transferred to a measuring plate.

Calculation of residual wash performance: The intensity of the stainswas measured using a Digi-eye (Tiff files), and Coloreye software wasused to acquire the intensity data. Using the Intensity data of thestored and fresh enzyme samples and the reference samples, Deltaperformance (Delta Intensity, DI) and Residual performance (RP) for eachstain were calculated. Briefly, for each enzyme combination DI wascalculated by subtracting the reference intensity from each measuredintensity. Furthermore, Residual performance was calculated for eachcombination of enzymes by the following formula:RP=DI_(stored)/DI_(Fresh)* 100%.

TABLE 34 Residual wash performance (RP) of XL (xanthan lyase) and EG(endoglucanase) enzyme combinations (wildtypes or variants) afterstorage 13 days in PUG detergent at 30° C. Tested on Mayo with soot(CS-05S) swatches. Enzyme mutations relative to SEQ ID NO: 2 Mayo withsoot, CS-05S (EG) and relative to SEQ ID NO: 6 (XL) Delta IntensityEndoglucanase (EG) Xanthan lyase (XL) Fresh Stored RP none, wildtypenone, wildtype 17 2 15% A559N + Y579W + T697G E229N + N672D + P752K +G753E + 16 5 30% A769D + L775A + D801G + K875T + N892Y A559N + Y579W +T697G E229S + N672D + G753E + S754E + 16 7 43% A769D + L775A + D801G +K875T + N892Y A559N + Y579W + T697G E229S + N672D + P752R + G753E + 19 844% S754E + A769D + L775A + D801G + K875T + N892Y A559N + Y579W + T697GA190Q + E229S + I234V + A624E + 18 8 43% N672D + G753E + S754E + A769D +L775A + D801G + K875T A559N + Y579W + T697G A190Q + E229S + T631N +N672D + 17 8 46% I703L + P752K + G753E + A769D + L775A + D801G + K875TA559N + Y579W + T697G A190Q + E229S + I234V + S582K + 19 12 65% N672D +G753E + S754E + S757D + A769D + L775A + D801G + K875T + N892Y A559N +Y579W + T697G E229S + N440K + S582K + A624E + 14 11 78% S635E + N672D +G738L + G753E + S754E + S757D + A769D + L775A + D801G + K875T + N892YK512P + A559N + Y579W + T697G E229S + N672D + G753E + S754E + 15 7 47%A769D + L775A + D801G + K875T + N892Y K512P + A559N + Y579W + T697GE229S + N672D + P752R + G753E + 17 9 54% S754E + A769D + L775A + D801G +K875T + N892Y K512P + A559N + Y579W + T697G A190Q + E229S + I234V +A624E + 16 8 48% N672D + G753E + S754E + A769D + L775A + D801G + K875TK512P + A559N + Y579W + T697G A190Q + E229S + T631N + N672D + 17 9 54%I703L + P752K + G753E + A769D + L775A + D801G + K875T K512P + A559N +Y579W + T697G A190Q + E229S + I234V + S582K + 17 11 66% N672D + G753E +S754E + S757D + A769D + L775A + D801G + K875T + N892Y K512P + A559N +Y579W + T697G E229S + N440K + S582K + A624E + 16 13 84% S635E + N672D +G738L + G753E + S754E + S757D + A769D + L775A + D801G + K875T + N892YN18G + A71E + A186P + E229S + N672D + G753E + S754E + 17 6 34% E408D +Y579W + I602T + A769D + L775A + D801G + K875T + A651P + A688G + V756YN892Y N18G + A71E + A186P + E229S + N672D + P752R + G753E + 17 7 44%E408D + Y579W + I602T + S754E + A769D + L775A + D801G + A651P + A688G +V756Y K875T + N892Y N18G + A71E + A186P + A190Q + E229S + I234V +A624E + 16 6 40% E408D + Y579W + I602T + N672D + G753E + S754E + A769D +A651P + A688G + V756Y L775A + D801G + K875T N18G + A71E + A186P +A190Q + E229S + T631N + N672D + 16 7 46% E408D + Y579W + 1602T + I703L +P752K + G753E + A769D + A651P + A688G + V756Y L775A + D801G + K875TN18G + A71E + A186P + A190Q + E229S + I234V + S582K + 15 10 69% E408D +Y579W + I602T + N672D + G753E + S754E + S757D + A651P + A688G + V756YA769D + L775A + D801G + K875T + N892Y N18G + A71E + A186P + E229S +N440K + S582K + A624E + 13 8 61% E408D + Y579W + I602T + S635E + N672D +G738L + G753E + A651P + A688G + V756Y S754E + S757D + A769D + L775A +D801G + K875T + N892Y N18G + N189K + E408D + E229N + N672D + P752K +G753E + 17 4 24% A559N + Y579W + A688G + A769D + L775A + D801G + K875T +T697G + V756Y + K921R + N892Y Y934G N18G + N189K + E408D + E229S +N672D + G753E + S754E + 15 5 31% A559N + Y579W + A688G + A769D + L775A +D801G + K875T + T697G + V756Y + K921R + N892Y Y934G N18G + N189K +E408D + E229S + N672D + P752R + G753E + 19 8 41% A559N + Y579W + A688G +S754E + A769D + L775A + D801G + T697G + V756Y + K921R + K875T + N892YY934G N18G + N189K + E408D + A190Q + E229S + I234V + A624E + 19 6 31%A559N + Y579W + A688G + N672D + G753E + S754E + A769D + T697G + V756Y +K921R + L775A + D801G + K875T Y934G N18G + N189K + E408D + A190Q +E229S + T631N + N672D + 17 8 44% A559N + Y579W + A688G + I703L + P752K +G753E + A769D + T697G + V756Y + K921R + L775A + D801G + K875T Y934GN18G + N189K + E408D + A190Q + E229S + I234V + S582K + 19 12 64% A559N +Y579W + A688G + N672D + G753E + S754E + S757D + T697G + V756Y + K921R +A769D + L775A + D801G + K875T + Y934G N892Y N18G + N189K + E408D +E229S + N440K + S582K + A624E + 17 14 84% A559N + Y579W + A688G +S635E + N672D + G738L + G753E + T697G + V756Y + K921R + S754E + S757D +A769D + L775A + Y934G D801G + K875T + N892Y S313D + E408D E229N +N672D + P752K + G753E + 17 5 31% A769D + L775A + D801G + K875T + N892YS313D + E408D E229S + N672D + G753E + S754E + 17 6 35% A769D + L775A +D801G + K875T + N892Y S313D + E408D E229S + N672D + P752R + G753E + 1910 51% S754E + A769D + L775A + D801G + K875T + N892Y S313D + E408DA190Q + E229S + I234V + A624E + 18 8 48% N672D + G753E + S754E + A769D +L775A + D801G + K875T S313D + E408D A190Q + E229S + T631N + N672D + 17 744% I703L + P752K + G753E + A769D + L775A + D801G + K875T S313D + E408DA190Q + E229S + I234V + S582K + 18 13 74% N672D + G753E + S754E +S757D + A769D + L775A + D801G + K875T + N892Y S313D + E408D E229S +N440K + S582K + A624E + 14 10 69% S635E + N672D + G738L + G753E +S754E + S757D + A769D + L775A + D801G + K875T + N892Y R880K + N905D +K921R + Y934G E229N + N672D + P752K + G753E + 18 5 29% A769D + L775A +D801G + K875T + N892Y R880K + N905D + K921R + Y934G E229S + N672D +G753E + S754E + 18 7 41% A769D + L775A + D801G + K875T + N892Y R880K +N905D + K921R + Y934G E229S + N672D + P752R + G753E + 20 8 37% S754E +A769D + L775A + D801G + K875T + N892Y R880K + N905D + K921R + Y934GA190Q + E229S + I234V + A624E + 17 8 47% N672D + G753E + S754E + A769D +L775A + D801G + K875T R880K + N905D + K921R + Y934G A190Q + E229S +T631N + N672D + 21 9 44% I703L + P752K + G753E + A769D + L775A + D801G +K875T R880K + N905D + K921R + Y934G A190Q + E229S + I234V + S582K + 2013 67% N672D + G753E + S754E + S757D + A769D + L775A + D801G + K875T +N892Y R880K + N905D + K921R + Y934G E229S + N440K + S582K + A624E + 1914 74% S635E + N672D + G738L + G753E + S754E + S757D + A769D + L775A +D801G + K875T + N892Y I302D + S313D + E408D + E229N + N672D + P752K +G753E + 17 4 24% Y579W + I602T + A651P + A769D + L775A + D801G + K875T +T697G + R880K + K921R + N892Y Y934G I302D + S313D + E408D + E229S +N672D + G753E + S754E + 17 6 36% Y579W + I602T + A651P + A769D + L775A +D801G + K875T + T697G + R880K + K921R + N892Y Y934G I302D + S313D +E408D + E229S + N672D + P752R + G753E + 18 8 45% Y579W + I602T + A651P +S754E + A769D + L775A + D801G + T697G + R880K + K921R + K875T + N892YY934G I302D + S313D + E408D + A190Q + E229S + I234V + A624E + 17 7 40%Y579W + I602T + A651P + N672D + G753E + S754E + A769D + T697G + R880K +K921R + L775A + D801G + K875T Y934G I302D + S313D + E408D + A190Q +E229S + T631N + N672D + 18 6 33% Y579W + I602T + A651P + I703L + P752K +G753E + A769D + T697G + R880K + K921R + L775A + D801G + K875T Y934GI302D + S313D + E408D + A190Q + E229S + I234V + S582K + 18 11 63%Y579W + I602T + A651P + N672D + G753E + S754E + S757D + T697G + R880K +K921R + A769D + L775A + D801G + K875T + Y934G N892Y I302D + S313D +E408D + E229S + N440K + S582K + A624E + 14 11 79% Y579W + I602T +A651P + S635E + N672D + G738L + G753E + T697G + R880K + K921R + S754E +S757D + A769D + L775A + Y934G D801G + K875T + N892Y N216Q + S313D +E408D + E229N + N672D + P752K + G753E + 16 10 61% D476R + Y579W +I602T + A769D + L775A + D801G + K875T + F638N + A651P + T697G + N892YW719R + R880K + T887K + K921R + Y934G N216Q + S313D + E408D + E229S +N672D + G753E + S754E + 17 11 63% D476R + Y579W + I602T + A769D +L775A + D801G + K875T + F638N + A651P + T697G + N892Y W719R + R880K +T887K + K921R + Y934G N216Q + S313D + E408D + E229S + N672D + P752R +G753E + 19 14 74% D476R + Y579W + I602T + S754E + A769D + L775A +D801G + F638N + A651P + T697G + K875T + N892Y W719R + R880K + T887K +K921R + Y934G N216Q + S313D + E408D + A190Q + E229S + I234V + A624E + 1712 73% D476R + Y579W + I602T + N672D + G753E + S754E + A769D + F638N +A651P + T697G + L775A + D801G + K875T W719R + R880K + T887K + K921R +Y934G N216Q + S313D + E408D + A190Q + E229S + T631N + N672D + 19 14 75%D476R + Y579W + I602T + I703L + P752K + G753E + A769D + F638N + A651P +T697G + L775A + D801G + K875T W719R + R880K + T887K + K921R + Y934GN216Q + S313D + E408D + A190Q + E229S + I234V + S582K + 20 16 80%D476R + Y579W + I602T + N672D + G753E + S754E + S757D + F638N + A651P +T697G + A769D + L775A + D801G + K875T + W719R + R880K + T887K + N892YK921R + Y934G N216Q + S313D + E408D + E229S + N440K + S582K + A624E + 1918 97% D476R + Y579W + I602T + S635E + N672D + G738L + G753E + F638N +A651P + T697G + S754E + S757D + A769D + L775A + W719R + R880K + T887K +D801G + K875T + N892Y K921R + Y934G

TABLE 35 Residual wash performance (RP) of XL and EG enzyme combinations(wildtypes or variants) after storage 13 days in PUG detergent at 30° C.Tested on Chocolate drink (CS-44) swatches. Enzyme mutations relative toSEQ ID NO: 2 Chocolate drink, CS-44 (EG) and relative to SEQ ID NO: 6(XL) Delta Intensity Endoglucanase (EG) Xanthan lyase (XL) Fresh StoredRP none, wildtype none, wildtype 14 3 23% A559N + Y579W + T697G E229N +N672D + P752K + G753E + 13 4 30% A769D + L775A + D801G + K875T + N892YA559N + Y579W + T697G E229S + N672D + G753E + S754E + 13 5 39% A769D +L775A + D801G + K875T + N892Y A559N + Y579W + T697G E229S + N672D +P752R + G753E + 16 6 41% S754E + A769D + L775A + D801G + K875T + N892YA559N + Y579W + T697G A190Q + E229S + I234V + A624E + 13 7 49% N672D +G753E + S754E + A769D + L775A + D801G + K875T+ A559N + Y579W + T697GA190Q + E229S + T631N + N672D + 15 6 40% I703L + P752K + G753E + A769D +L775A + D801G + K875T+ A559N + Y579W + T697G A190Q + E229S + I234V +S582K + 12 9 75% N672D + G753E + S754E + S757D + A769D + L775A + D801G +K875T + N892Y A559N + Y579W + T697G E229S + N440K + S582K + A624E + 10 556% S635E + N672D + G738L + G753E + S754E + S757D + A769D + L775A +D801G + K875T + N892Y K512P + A559N + Y579W + T697G E229S + N672D +G753E + S754E + 11 4 39% A769D + L775A + D801G + K875T + N892Y K512P +A559N + Y579W + T697G E229S + N672D + P752R + G753E + 12 6 52% S754E +A769D + L775A + D801G + K875T + N892Y K512P + A559N + Y579W + T697GA190Q + E229S + I234V + A624E + 12 5 44% N672D + G753E + S754E + A769D +L775A + D801G + K875T+ K512P + A559N + Y579W + T697G A190Q + E229S +T631N + N672D + 13 8 63% I703L + P752K + G753E + A769D + L775A + D801G +K875T+ K512P + A559N + Y579W + T697G A190Q + E229S + I234V + S582K + 1412 84% N672D + G753E + S754E + S757D + A769D + L775A + D801G + K875T +N892Y K512P + A559N + Y579W + T697G E229S + N440K + S582K + A624E + 11 977% S635E + N672D + G738L + G753E + S754E + S757D + A769D + L775A +D801G + K875T + N892Y N18G + A71E + A186P + E408D + E229S + N672D +G753E + S754E + 15 6 40% Y579W + I602T + A651P + A688G + A769D + L775A +D801G + K875T + V756Y N892Y N18G + A71E + A186P + E408D + A190Q +E229S + I234V + A624E + 16 6 37% Y579W + I602T + A651P + A688G + N672D +G753E + S754E + A769D + V756Y L775A + D801G + K875T+ N18G + A71E +A186P + E408D + A190Q + E229S + T631N + N672D + 16 5 29% Y579W + I602T +A651P + A688G + I703L + P752K + G753E + A769D + V756Y L775A + D801G +K875T+ N18G + A71E + A186P + E408D + A190Q + E229S + I234V + S582K + 137 50% Y579W + I602T + A651P + A688G + N672D + G753E + S754E + S757D +V756Y A769D + L775A + D801G + K875T + N892Y N18G + A71E + A186P +E408D + E229S + N440K + S582K + A624E + 8 6 81% Y579W + I602T + A651P +A688G + S635E + N672D + G738L + G753E + V756Y S754E + S757D + A769D +L775A + D801G + K875T + N892Y N18G + N189K + E408D + A559N + E229S +N672D + G753E + S754E + 14 4 26% Y579W + A688G + T697G + V756Y + A769D +L775A + D801G + K875T + K921R + Y934G N892Y N18G + N189K + E408D +A559N + E229S + N672D + P752R + G753E + 13 3 25% Y579W + A688G + T697G +V756Y + S754E + A769D + L775A + D801G + K921R + Y934G K875T + N892YN18G + N189K + E408D + A559N + A190Q + E229S + T631N + N672D + 16 7 46%Y579W + A688G + T697G + V756Y + I703L + P752K + G753E + A769D + K921R +Y934G L775A + D801G + K875T+ N18G + N189K + E408D + A559N + A190Q +E229S + I234V + S582K + 15 8 55% Y579W + A688G + T697G + V756Y + N672D +G753E + S754E + S757D + K921R + Y934G A769D + L775A + D801G + K875T +N892Y N18G + N189K + E408D + A559N + E229S + N440K + S582K + A624E + 136 51% Y579W + A688G + T697G + V756Y + S635E + N672D + G738L + G753E +K921R + Y934G S754E + S757D + A769D + L775A + D801G + K875T + N892YS313D + E408D E229N + N672D + P752K + G753E + 14 3 24% A769D + L775A +D801G + K875T + N892Y S313D + E408D E229S + N672D + G753E + S754E + 15 428% A769D + L775A + D801G + K875T + N892Y S313D + E408D A190Q + E229S +I234V + A624E + 15 3 23% N672D + G753E + S754E + A769D + L775A + D801G +K875T+ S313D + E408D A190Q + E229S + T631N + N672D + 14 6 40% I703L +P752K + G753E + A769D + L775A + D801G + K875T+ S313D + E408D A190Q +E229S + I234V + S582K + 13 8 59% N672D + G753E + S754E + S757D + A769D +L775A + D801G + K875T + N892Y S313D + E408D E229S + N440K + S582K +A624E + 10 8 79% S635E + N672D + G738L + G753E + S754E + S757D + A769D +L775A + D801G + K875T + N892Y R880K + N905D + K921R + Y934G E229N +N672D + P752K + G753E + 13 4 33% A769D + L775A + D801G + K875T + N892YR880K + N905D + K921R + Y934G E229S + N672D + P752R + G753E + 16 6 36%S754E + A769D + L775A + D801G + K875T + N892Y R880K + N905D + K921R +Y934G A190Q + E229S + I234V + A624E + 15 6 36% N672D + G753E + S754E +A769D + L775A + D801G + K875T+ R880K + N905D + K921R + Y934G A190Q +E229S + T631N + N672D + 16 5 31% I703L + P752K + G753E + A769D + L775A +D801G + K875T+ R880K + N905D + K921R + Y934G A190Q + E229S + I234V +S582K + 14 12 82% N672D + G753E + S754E + S757D + A769D + L775A +D801G + K875T + N892Y R880K + N905D + K921R + Y934G E229S + N440K +S582K + A624E + 15 9 64% S635E + N672D + G738L + G753E + S754E + S757D +A769D + L775A + D801G + K875T + N892Y I302D + S313D + E408D + Y579W +E229N + N672D + P752K + G753E + 16 6 38% I602T + A651P + T697G + R880K +A769D + L775A + D801G + K875T + K921R + Y934G N892Y I302D + S313D +E408D + Y579W + E229S + N672D + G753E + S754E + 18 4 23% I602T + A651P +T697G + R880K + A769D + L775A + D801G + K875T + K921R + Y934G N892YI302D + S313D + E408D + Y579W + E229S + N672D + P752R + G753E + 16 6 40%I602T + A651P + T697G + R880K + S754E + A769D + L775A + D801G + K921R +Y934G K875T + N892Y I302D + S313D + E408D + Y579W + A190Q + E229S +I234V + A624E + 17 5 29% I602T + A651P + T697G + R880K + N672D + G753E +S754E + A769D + K921R + Y934G L775A + D801G + K875T+ I302D + S313D +E408D + Y579W + A190Q + E229S + T631N + N672D + 18 5 27% I602T + A651P +T697G + R880K + I703L + P752K + G753E + A769D + K921R + Y934G L775A +D801G + K875T+ I302D + S313D + E408D + Y579W + A190Q + E229S + I234V +S582K + 17 8 45% I602T + A651P + T697G + R880K + N672D + G753E + S754E +S757D + K921R + Y934G A769D + L775A + D801G + K875T + N892Y I302D +S313D + E408D + Y579W + E229S + N440K + S582K + A624E + 12 9 70% I602T +A651P + T697G + R880K + S635E + N672D + G738L + G753E + K921R + Y934GS754E + S757D + A769D + L775A + D801G + K875T + N892Y N216Q + S313D +E408D + D476R + E229N + N672D + P752K + G753E + 14 9 65% Y579W + I602T +F638N + A651P + A769D + L775A + D801G + K875T + T697G + W719R + R880K +T887K + N892Y K921R + Y934G N216Q + S313D + E408D + D476R + E229S +N672D + G753E + S754E + 14 10 66% Y579W + I602T + F638N + A651P +A769D + L775A + D801G + K875T + T697G + W719R + R880K + T887K + N892YK921R + Y934G N216Q + S313D + E408D + D476R + E229S + N672D + P752R +G753E + 16 14 84% Y579W + I602T + F638N + A651P + S754E + A769D +L775A + D801G + T697G + W719R + R880K + T887K + K875T + N892Y K921R +Y934G N216Q + S313D + E408D + D476R + A190Q + E229S + I234V + A624E + 1614 90% Y579W + I602T + F638N + A651P + N672D + G753E + S754E + A769D +T697G + W719R + R880K + T887K + L775A + D801G + K875T+ K921R + Y934GN216Q + S313D + E408D + D476R + A190Q + E229S + T631N + N672D + 18 1374% Y579W + I602T + F638N + A651P + I703L + P752K + G753E + A769D +T697G + W719R + R880K + T887K + L775A + D801G + K875T+ K921R + Y934GN216Q + S313D + E408D + D476R + A190Q + E229S + I234V + S582K + 19 1369% Y579W + I602T + F638N + A651P + N672D + G753E + S754E + S757D +T697G + W719R + R880K + T887K + A769D + L775A + D801G + K875T + K921R +Y934G N892Y N216Q + S313D + E408D + D476R + E229S + N440K + S582K +A624E + 18 16 92% Y579W + I602T + F638N + A651P + S635E + N672D +G738L + G753E + T697G + W719R + R880K + T887K + S754E + S757D + A769D +L775A + K921R + Y934G D801G + K875T + N892Y

TABLE 36 Residual wash performance (RP) of XL and EG enzyme combinations(wildtypes or variants) after storage 13 days in PUG detergent at 30° C.Tested on Fluid Make up (CS-17) swatches. Enzyme mutations relative toSEQ ID NO: 2 Fluid Make up, CS-17 (EG) and relative to SEQ ID NO: 6 (XL)Delta Intensity Endoglucanase (EG) Xanthan lyase (XL) Fresh Stored RPnone, wildtype none, wildtype 9 0  3% A559N + Y579W + T697G E229N +N672D + P752K + G753E + 10 3 29% A769D + L775A + D801G + K875T + N892YA559N + Y579W + T697G E229S + N672D + G753E + S754E + 10 4 39% A769D +L775A + D801G + K875T + N892Y A559N + Y579W + T697G E229S + N672D +P752R + G753E + 12 6 52% S754E + A769D + L775A + D801G + K875T + N892YA559N + Y579W + T697G A190Q + E229S + I234V + A624E + 12 4 32% N672D +G753E + S754E + A769D + L775A + D801G + K875T+ A559N + Y579W + T697GA190Q + E229S + T631N + N672D + 11 5 40% I703L + P752K + G753E + A769D +L775A + D801G + K875T+ A559N + Y579W + T697G A190Q + E229S + I234V +S582K + 12 8 65% N672D + G753E + S754E + S757D + A769D + L775A + D801G +K875T + N892Y A559N + Y579W + T697G E229S + N440K + S582K + A624E + 8 562% S635E + N672D + G738L + G753E + S754E + S757D + A769D + L775A +D801G + K875T + N892Y K512P + A559N + Y579W + T697G E229S + N672D +G753E + S754E + 9 4 49% A769D + L775A + D801G + K875T + N892Y K512P +A559N + Y579W + T697G E229S + N672D + P752R + G753E + 10 4 34% S754E +A769D + L775A + D801G + K875T + N892Y K512P + A559N + Y579W + T697GA190Q + E229S + I234V + A624E + 10 4 37% N672D + G753E + S754E + A769D +L775A + D801G + K875T+ K512P + A559N + Y579W + T697G A190Q + E229S +T631N + N672D + 12 4 33% I703L + P752K + G753E + A769D + L775A + D801G +K875T+ K512P + A559N + Y579W + T697G A190Q + E229S + I234V + S582K + 107 69% N672D + G753E + S754E + S757D + A769D + L775A + D801G + K875T +N892Y K512P + A559N + Y579W + T697G E229S + N440K + S582K + A624E + 10 765% S635E + N672D + G738L + G753E + S754E + S757D + A769D + L775A +D801G + K875T + N892Y N18G + A71E + A186P + E408D + E229S + N672D +G753E + S754E + 8 3 33% Y579W + I602T + A651P + A688G + A769D + L775A +D801G + K875T + V756Y N892Y N18G + A71E + A186P + E408D + E229S +N672D + P752R + G753E + 8 4 54% Y579W + I602T + A651P + A688G + S754E +A769D + L775A + D801G + V756Y K875T + N892Y N18G + A71E + A186P +E408D + A190Q + E229S + I234V + A624E + 8 4 56% Y579W + I602T + A651P +A688G + N672D + G753E + S754E + A769D + V756Y L775A + D801G + K875T+N18G + A71E + A186P + E408D + A190Q + E229S + T631N + N672D + 8 5 62%Y579W + I602T + A651P + A688G + I703L + P752K + G753E + A769D + V756YL775A + D801G + K875T+ N18G + A71E + A186P + E408D + A190Q + E229S +I234V + S582K + 7 5 76% Y579W + I602T + A651P + A688G + N672D + G753E +S754E + S757D + V756Y A769D + L775A + D801G + K875T + N892Y N18G +A71E + A186P + E408D + E229S + N440K + S582K + A624E + 4 4 81% Y579W +I602T + A651P + A688G + S635E + N672D + G738L + G753E + V756Y S754E +S757D + A769D + L775A + D801G + K875T + N892Y N18G + N189K + E408D +A559N + E229N + N672D + P752K + G753E + 6 2 32% Y579W + A688G + T697G +V756Y + A769D + L775A + D801G + K875T + K921R + Y934G N892Y N18G +N189K + E408D + A559N + E229S + N672D + G753E + S754E + 6 4 61% Y579W +A688G + T697G + V756Y + A769D + L775A + D801G + K875T + K921R + Y934GN892Y N18G + N189K + E408D + A559N + E229S + N672D + P752R + G753E + 8 447% Y579W + A688G + T697G + V756Y + S754E + A769D + L775A + D801G +K921R + Y934G K875T + N892Y N18G + N189K + E408D + A559N + A190Q +E229S + I234V + A624E + 8 3 37% Y579W + A688G + T697G + V756Y + N672D +G753E + S754E + A769D + K921R + Y934G L775A + D801G + K875T+ N18G +N189K + E408D + A559N + A190Q + E229S + T631N + N672D + 7 5 63% Y579W +A688G + T697G + V756Y + I703L + P752K + G753E + A769D + K921R + Y934GL775A + D801G + K875T+ N18G + N189K + E408D + A559N + A190Q + E229S +I234V + S582K + 8 7 87% Y579W + A688G + T697G + V756Y + N672D + G753E +S754E + S757D + K921R + Y934G A769D + L775A + D801G + K875T + N892YN18G + N189K + E408D + A559N + E229S + N440K + S582K + A624E + 7 6 96%Y579W + A688G + T697G + V756Y + S635E + N672D + G738L + G753E + K921R +Y934G S754E + S757D + A769D + L775A + D801G + K875T + N892Y S313D +E408D E229N + N672D + P752K + G753E + 10 5 49% A769D + L775A + D801G +K875T + N892Y S313D + E408D E229S + N672D + G753E + S754E + 9 4 47%A769D + L775A + D801G + K875T + N892Y S313D + E408D E229S + N672D +P752R + G753E + 10 5 53% S754E + A769D + L775A + D801G + K875T + N892YS313D + E408D A190Q + E229S + I234V + A624E + 10 5 47% N672D + G753E +S754E + A769D + L775A + D801G + K875T+ S313D + E408D A190Q + E229S +T631N + N672D + 11 5 49% I703L + P752K + G753E + A769D + L775A + D801G +K875T+ S313D + E408D A190Q + E229S + I234V + S582K + 9 8 91% N672D +G753E + S754E + S757D + A769D + L775A + D801G + K875T + N892Y S313D +E408D E229S + N440K + S582K + A624E + 7 6 83% S635E + N672D + G738L +G753E + S754E + S757D + A769D + L775A + D801G + K875T + N892Y R880K +N905D + K921R + Y934G E229N + N672D + P752K + G753E + 8 3 32% A769D +L775A + D801G + K875T + N892Y R880K + N905D + K921R + Y934G E229S +N672D + G753E + S754E + 10 6 57% A769D + L775A + D801G + K875T + N892YR880K + N905D + K921R + Y934G E229S + N672D + P752R + G753E + 9 2 26%S754E + A769D + L775A + D801G + K875T + N892Y R880K + N905D + K921R +Y934G A190Q + E229S + I234V + A624E + 11 5 44% N672D + G753E + S754E +A769D + L775A + D801G + K875T+ R880K + N905D + K921R + Y934G A190Q +E229S + T631N + N672D + 11 5 45% I703L + P752K + G753E + A769D + L775A +D801G + K875T+ R880K + N905D + K921R + Y934G A190Q + E229S + I234V +S582K + 11 8 72% N672D + G753E + S754E + S757D + A769D + L775A + D801G +K875T + N892Y R880K + N905D + K921R + Y934G E229S + N440K + S582K +A624E + 10 8 79% S635E + N672D + G738L + G753E + S754E + S757D + A769D +L775A + D801G + K875T + N892Y I302D + S313D + E408D + Y579W + E229N +N672D + P752K + G753E + 10 3 33% I602T + A651P + T697G + R880K + A769D +L775A + D801G + K875T + K921R + Y934G N892Y I302D + S313D + E408D +Y579W + E229S + N672D + G753E + S754E + 9 2 18% I602T + A651P + T697G +R880K + A769D + L775A + D801G + K875T + K921R + Y934G N892Y I302D +S313D + E408D + Y579W + E229S + N672D + P752R + G753E + 9 5 55% I602T +A651P + T697G + R880K + S754E + A769D + L775A + D801G + K921R + Y934GK875T + N892Y I302D + S313D + E408D + Y579W + A190Q + E229S + I234V +A624E + 10 4 35% I602T + A651P + T697G + R880K + N672D + G753E + S754E +A769D + K921R + Y934G L775A + D801G + K875T+ I302D + S313D + E408D +Y579W + A190Q + E229S + T631N + N672D + 10 5 47% I602T + A651P + T697G +R880K + I703L + P752K + G753E + A769D + K921R + Y934G L775A + D801G +K875T+ I302D + S313D + E408D + Y579W + A190Q + E229S + I234V + S582K + 96 61% I602T + A651P + T697G + R880K + N672D + G753E + S754E + S757D +K921R + Y934G A769D + L775A + D801G + K875T + N892Y I302D + S313D +E408D + Y579W + E229S + N440K + S582K + A624E + 7 9 120%  I602T +A651P + T697G + R880K + S635E + N672D + G738L + G753E + K921R + Y934GS754E + S757D + A769D + L775A + D801G + K875T + N892Y N216Q + S313D +E408D + D476R + E229N + N672D + P752K + G753E + 8 8 94% Y579W + I602T +F638N + A651P + A769D + L775A + D801G + K875T + T697G + W719R + R880K +T887K + N892Y K921R + Y934G N216Q + S313D + E408D + D476R + E229S +N672D + G753E + S754E + 10 9 92% Y579W + I602T + F638N + A651P + A769D +L775A + D801G + K875T + T697G + W719R + R880K + T887K + N892Y K921R +Y934G N216Q + S313D + E408D + D476R + E229S + N672D + P752R + G753E + 910 117%  Y579W + I602T + F638N + A651P + S754E + A769D + L775A + D801G +T697G + W719R + R880K + T887K + K875T + N892Y K921R + Y934G N216Q +S313D + E408D + D476R + A190Q + E229S + I234V + A624E + 10 9 94% Y579W +I602T + F638N + A651P + N672D + G753E + S754E + A769D + T697G + W719R +R880K + T887K + L775A + D801G + K875T+ K921R + Y934G N216Q + S313D +E408D + D476R + A190Q + E229S + T631N + N672D + 10 9 93% Y579W + I602T +F638N + A651P + I703L + P752K + G753E + A769D + T697G + W719R + R880K +T887K + L775A + D801G + K875T+ K921R + Y934G N216Q + S313D + E408D +D476R + A190Q + E229S + I234V + S582K + 9 11 122%  Y579W + I602T +F638N + A651P + N672D + G753E + S754E + S757D + T697G + W719R + R880K +T887K + A769D + L775A + D801G + K875T + K921R + Y934G N892Y N216Q +S313D + E408D + D476R + E229S + N440K + S582K + A624E + 10 13 125% Y579W + I602T + F638N + A651P + S635E + N672D + G738L + G753E + T697G +W719R + R880K + T887K + S754E + S757D + A769D + L775A + K921R + Y934GD801G + K875T + N892Y

Example 11 Washing Test

TABLE 37 Liquid laundry detergent matrix: % Active Matter % ActiveMatter raw material in Formula Water demin. 100  ad 100% ProteaseStabilisator 100 0.8-1.2% Citric acid 100  2.5-4% Antifoaming agent t.q.0.02-0.05%  FAEOS (anionic surfactant) 70 7.5-9.5% FAEO (nonionicsurfactant) 100 5.5-7.5% LAS (anionic surfactant) 96    6-8% palm kerneloil fatty acid 30 2.5-4.5% NaOH 50 2.5-3.5% 1,2-Propandiol 100    4-7%DTPMP-xNa 40  0.5-1% Soil repellent 70  1-1.8% Ethanol 93    1-4%Optical brightener 90  0.05-2% Enzyme Mix 100 1.4-1.8% Perfume 1000.5-1.4% Dye tq 0.001-0.004%   Dosage: 73 mL pH: 8.2-8.6

The enzymes to be tested were combined with the above liquid laundrydetergent matrix in the concentrations given below, homogenized andstored in a closed container at 30° C. for 8 weeks. The freshly preparedmatrix including the enzymes and the matrix with the enzymes stored for8 weeks at 30° C. were used in the following wash test:

The wash test was performed under standard conditions (40° C., colorprogram, 16° dH) and the brightness of several different commercialstains determined after drying and ironing via brightness measurement.To the stain sets, 3.5 kg clean cotton textiles and 4 SBL sheets wereadded.

Stain Set: CFT CS44 [CO] Chocolate drink CFT CS06 [CO] Salad dressingCFT CS05S [CO] Mayonaise CFT CS02 [CO] Cocoa EMPA 165 [CO] chocolatepudding

Every wash test was performed 6 times and the average values calculated.

The enzymes were used in active protein concentrations of 102 mg xanthanlyase and 1.53 mg endoglucanase. The following enzyme combinations weretested:

Combination WT: Xanthan lyase WT (SEQ ID NO:6) and endoglucanase WT (SEQID NO:2)

Combination 1: Xanthan lyase variant 1 (SEQ ID NO:6 with the followingsubstitutions:A190Q+E229S+I234V+S582K+N672D+G753E+S754E+S757D+A769D+L775A+D801G+K875T+N892Y) and endoglucanase variant 2 (SEQ ID NO:2 with the followingsubstitutions:N216Q+5313D+E408D+D476R+Y579W+I602T+F638N+A651P+T697G+W719R+R880K+T887K+K921R+Y934G)

Combination 2: Xanthan lyase variant 2 (SEQ ID NO:6 withE229S+N440K+S582K+A624E+S635E+N672D+G738L+G753E+S754E+S757D+A769D+L775A+D801G+K875T+N892Y) and endoglucanase variant 1 (SEQ ID NO:2 withF20P+S313D+E408D+Y579W+S636K+A688G+T697G+N905D+A937E)

Combination 3: Xanthan lyase variant 3 (SEQ ID NO:6 withS100D+E229S+K360G+D458S+S582K+N672D+G753E+S754E+S757D+A769D+L775A+D801G+A843P+K875T+N892Y+N1008D) and endoglucanase variant 2 (SEQ ID NO:2 withN216Q+S313D+E408D+D476R+Y579W+I602T+F638N+A651P+T697G+W719R+R880K+T887K+K921R+Y934G)

In the following table Delta Y values of the inventive formulations aregiven relative to the detergent without enzymes, each as the sum of the5 given stains. The higher the value the better the cleaning performanceof the respective enzyme combination.

TABLE 38 Washing test results Enzyme Performance Performance aftercombination fresh 8 weeks at 30° C. WT 26.2 0 1 26.4 16.6 2 26.6 17.9 322.1 16.0

As can be seen from the results, the enzyme combinations of theinvention provide for a significantly better performance after extendedstorage at 30° C.

1. A detergent composition comprising: (A) an endoglucanase variantcomprising an alteration at one or more positions in a region selectedfrom the group consisting of: region 1 corresponding to amino acids 95to 105 of SEQ ID NO: 2, region 2 corresponding to amino acids 115 to 138of SEQ ID NO: 2, region 3 corresponding to amino acids 210 to 251 of SEQID NO: 2, region 4 corresponding to amino acids 267 to 301 of SEQ ID NO:2, region 5 corresponding to amino acids 339 to 361 of SEQ ID NO: 2,region 6 corresponding to amino acids 547 to 595 of SEQ ID NO: 2, region7 corresponding to amino acids 612 to 660 of SEQ ID NO: 2, region 8corresponding to amino acids 806 to 828 of SEQ ID NO: 2, region 9corresponding to amino acids 839 to 1042 of SEQ ID NO: 2, region 10corresponding to amino acids 1 to 94 of SEQ ID NO: 2, region 11corresponding to amino acids 106 to 114 of SEQ ID NO: 2, region 12corresponding to amino acids 139 to 209 of SEQ ID NO: 2, region 13corresponding to amino acids 252 to 266 of SEQ ID NO: 2, region 14corresponding to amino acids 302 to 338 of SEQ ID NO: 2, region 15corresponding to amino acids 362 to 546 of SEQ ID NO: 2, region 16corresponding to amino acids 596 to 611 of SEQ ID NO: 2, region 17corresponding to amino acids 661 to 805 of SEQ ID NO: 2, region 18corresponding to amino acids 829 to 838 of SEQ ID NO: 2, and region 19corresponding to amino acids 1043 to 1055 of SEQ ID NO: 2; and (B) axanthan lyase variant comprising an alterationat one or more positionsin a region selected from the group consisting of: region 1corresponding to amino acids 154 to 176 of SEQ ID NO: 6, region 2corresponding to amino acids 614 to 658 of SEQ ID NO: 6, region 3corresponding to amino acids 731 to 803 of SEQ ID NO: 6, region 4corresponding to amino acids 807 to 846 of SEQ ID NO: 6, region 5corresponding to amino acids 872 to 885 of SEQ ID NO: 6, region 6corresponding to amino acids 903 to 1004 of SEQ ID NO: 6, region 7corresponding to amino acids 1 to 153 of SEQ ID NO: 6, region 8corresponding to amino acids 177 to 613 of SEQ ID NO: 6, region 9corresponding to amino acids 659 to 730 of SEQ ID NO: 6, region 10corresponding to amino acids 804 to 806 of SEQ ID NO: 6, region 11corresponding to amino acids 847 to 871 of SEQ ID NO: 6, region 12corresponding to amino acids 886 to 902 of SEQ ID NO: 6, and region 13corresponding to amino acids 1005 to 1037 of SEQ ID NO: 6; wherein theendoglucanase variant (A) has at least 60% and less than 100% sequenceidentity to SEQ ID NO: 2; and wherein the xanthan lyase variant (B) hasat least 60% and less than 100% sequence identity to SEQ ID NO.
 2. Thedetergent composition according to claim 1, wherein said endoglucanasevariant comprises an alteration at one or more positions in a regionselected from the group consisting of: i) region 1 corresponding toamino acids 95 to 105 of SEQ ID NO: 2, said alteration being at one ormore positions selected from the group consisting of positions: 95, 96,97, 98, 99, 100, 101, 102, 103, 104, 105, wherein said positionscorrespond to amino acid positions of SEQ ID NO: 2 according to thenumbering of SEQ ID NO: 2, ii) region 2 corresponding to amino acids 115to 138 of SEQ ID NO: 2, said alteration being at one or more positionsselected from the group consisting of positions: 115, 116, 117, 118,119, 120, 121, 122, 123, 124, 125, 126, 127, 128, 129, 130, 131, 132,133, 134, 135, 136, 137, 138, wherein said positions correspond to aminoacid positions of SEQ ID NO: 2 according to the numbering of SEQ ID NO:2, iii) region 3 corresponding to amino acids 210 to 251 of SEQ ID NO:2, said alteration being at one or more positions selected from thegroup consisting of positions: 210, 211, 212, 213, 214, 215, 216, 217,218, 219, 220, 221, 222, 223, 224, 225, 226, 227, 228, 229, 230, 231,232, 233, 234, 235, 236, 237, 238, 239, 240, 241, 242, 243, 244, 245,246, 247, 248, 249, 250, 251, wherein said positions correspond to aminoacid positions of SEQ ID NO: 2 according to the numbering of SEQ ID NO:2, iv) region 4 corresponding to amino acids 267 to 301 of SEQ ID NO: 2,said alteration being at one or more positions selected from the groupconsisting of positions: 267, 268, 269, 270, 271, 272, 273, 274, 275,276, 277, 278, 279, 280, 281, 282, 283, 284, 285, 286, 287, 288, 289,290, 291, 292, 293, 294, 295, 296, 297, 298, 299, 300, 301, wherein saidpositions correspond to amino acid positions of SEQ ID NO: 2 accordingto the numbering of SEQ ID NO: 2, v) region 5 corresponding to aminoacids 339 to 361 of SEQ ID NO: 2, said alteration being at one or morepositions selected from the group consisting of positions: 339, 340,341, 342, 343, 344, 345, 346, 347, 348, 349, 350, 351, 352, 353, 354,355, 356, 357, 358, 359, 360, 361, wherein said positions correspond toamino acid positions of SEQ ID NO: 2 according to the numbering of SEQID NO: 2, vi) region 6 corresponding to amino acids 547 to 595 of SEQ IDNO: 2, said alteration being at one or more positions selected from thegroup consisting of positions: 547, 548, 549, 550, 551, 552, 553, 554,555, 556, 557, 558, 559, 560, 561, 562, 563, 564, 565, 566, 567, 568,569, 570, 571, 572, 573, 574, 575, 576, 577, 578, 579, 580, 581, 582,583, 584, 585, 586, 587, 588, 589, 590, 591, 592, 593, 594, 595, whereinsaid positions correspond to amino acid positions of SEQ ID NO: 2according to the numbering of SEQ ID NO: 2, vii) region 7 correspondingto amino acids 612 to 660 of SEQ ID NO: 2, said alteration being at oneor more positions selected from the group consisting of positions: 612,613, 614, 615, 616, 617, 618, 619, 620, 621, 622, 623, 624, 625, 626,627, 628, 629, 630, 631, 632, 633, 634, 635, 636, 637, 638, 639, 640,641, 642, 643, 644, 645, 646, 647, 648, 649, 650, 651, 652, 653, 654,655, 656, 657, 658, 659, 660, wherein said positions correspond to aminoacid positions of SEQ ID NO: 2 according to the numbering of SEQ ID NO:2, viii) region 8 corresponding to amino acids 806 to 828 of SEQ ID NO:2, said alteration being at one or more positions selected from thegroup consisting of positions: 806, 807, 808, 809, 810, 811, 812, 813,814, 815, 816, 817, 818, 819, 820, 821, 822, 823, 824, 825, 826, 827,828, wherein said positions correspond to amino acid positions of SEQ IDNO: 2 according to the numbering of SEQ ID NO: 2, ix) region 9corresponding to amino acids 839 to 1042 of SEQ ID NO: 2, saidalteration being at one or more positions selected from the groupconsisting of positions: 839, 840, 841, 842, 843, 844, 845, 846, 847,848, 849, 850, 851, 852, 853, 854, 855, 856, 857, 858, 859, 860, 861,862, 863, 864, 865, 866, 867, 868, 869, 870, 871, 872, 873, 874, 875,876, 877, 878, 879, 880, 881, 882, 883, 884, 885, 886, 887, 888, 889,890, 891, 892, 893, 894, 895, 896, 897, 898, 899, 900, 901, 902, 903,904, 905, 906, 907, 908, 909, 910, 911, 912, 913, 914, 915, 916, 917,918, 919, 920, 921, 922, 923, 924, 925, 926, 927, 928, 929, 930, 931,932, 933, 934, 935, 936, 937, 938, 939, 940, 941, 942, 943, 944, 945,946, 947, 948, 949, 950, 951, 952, 953, 954, 955, 956, 957, 958, 959,960, 961, 962, 963, 964, 965, 966, 967, 968, 969, 970, 971, 972, 973,974, 975, 976, 977, 978, 979, 980, 981, 982, 983, 984, 985, 986, 987,988, 989, 990, 991, 992, 993, 994, 995, 996, 997, 998, 999, 1000, 1001,1002, 1003, 1004, 1005, 1006, 1007, 1008, 1009, 1010, 1011, 1012, 1013,1014, 1015, 1016, 1017, 1018, 1019, 1020, 1021, 1022, 1023, 1024, 1025,1026, 1027, 1028, 1029, 1030, 1031, 1032, 1033, 1034, 1035, 1036, 1037,1038, 1039, 1040, 1041, 1042, wherein said positions correspond to aminoacid positions of SEQ ID NO: 2 according to the numbering of SEQ ID NO:2, x) region 10 corresponding to amino acids 1 to 94 of SEQ ID NO: 2,said alteration being at one or more positions selected from the groupconsisting of positions: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14,15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32,33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50,51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68,69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86,87, 88, 89, 90, 91, 92, 93, 94, wherein said positions correspond toamino acid positions of SEQ ID NO: 2 according to the numbering of SEQID NO: 2, xi) region 11 corresponding to amino acids 106 to 114 of SEQID NO: 2, said alteration being at one or more positions selected fromthe group consisting of positions: 107, 108, 109, 110, 111, 112, 113,114, wherein said positions correspond to amino acid positions of SEQ IDNO: 2 according to the numbering of SEQ ID NO: 2[[)]], xii) region 12corresponding to amino acids 139 to 209 of SEQ ID NO: 2, said alterationbeing at one or more positions selected from the group consisting ofpositions: 139, 140, 141, 142, 143, 144, 145, 146, 147, 148, 149, 150,151, 152, 153, 154, 155, 156, 157, 158, 159, 160, 161, 162, 163, 164,165, 166, 167, 168, 169, 170, 171, 172, 173, 174, 175, 176, 177, 178,179, 180, 181, 182, 183, 184, 185, 186, 187, 188, 189, 190, 191, 192,193, 194, 195, 196, 197, 198, 199, 200, 201, 202, 203, 204, 205, 206,207, 208, 209, wherein said positions correspond to amino acid positionsof SEQ ID NO: 2 according to the numbering of SEQ ID NO: 2, xiii) region13 corresponding to amino acids 252 to 266 of SEQ ID NO: 2, saidalteration being at one or more positions selected from the groupconsisting of positions: 252, 253, 254, 255, 256, 257, 258, 259, 260,261, 262, 263, 264, 265, 266, wherein said positions correspond to aminoacid positions of SEQ ID NO: 2 according to the numbering of SEQ ID NO:2, xiv) region 14 corresponding to amino acids 302 to 338 of SEQ ID NO:2, said alteration being at one or more positions selected from thegroup consisting of positions: 302, 303, 304, 305, 306, 307, 308, 309,310, 311, 312, 313, 314, 315, 316, 317, 318, 319, 320, 321, 322, 323,324, 325, 326, 327, 328, 329, 330, 331, 332, 333, 334, 335, 336, 337,338, wherein said positions correspond to amino acid positions of SEQ IDNO: 2 according to the numbering of SEQ ID NO: 2, xv) region 15corresponding to amino acids 362 to 546 of SEQ ID NO: 2, said alterationbeing at one or more positions selected from the group consisting ofpositions: 362, 363, 364, 365, 366, 367, 368, 369, 370, 371, 372, 373,374, 375, 376, 377, 378, 379, 380, 381, 382, 383, 384, 385, 386, 387,388, 389, 390, 391, 392, 393, 394, 395, 396, 397, 398, 399, 400, 401,402, 403, 404, 405, 406, 407, 408, 409, 410, 411, 412, 413, 414, 415,416, 417, 418, 419, 420, 421, 422, 423, 424, 425, 426, 427, 428, 429,430, 431, 432, 433, 434, 435, 436, 437, 438, 439, 440, 441, 442, 443,444, 445, 446, 447, 448, 449, 450, 451, 452, 453, 454, 455, 456, 457,458, 459, 460, 461, 462, 463, 464, 465, 466, 467, 468, 469, 470, 471,472, 473, 474, 475, 476, 477, 478, 479, 480, 481, 482 ,483, 484, 485,486, 487, 488, 489, 490, 491, 492, 493, 494, 495, 496, 497, 498, 499,500, 501, 502, 503, 504, 505, 506, 507, 508, 509, 510, 511, 512, 513,514, 515, 516, 517, 518, 519, 520, 521, 522, 523, 524, 525, 526, 527,528, 529, 530, 531, 532, 533, 534, 535, 536, 537, 538, 539, 540, 541,542, 543, 544, 545, 546, wherein said positions correspond to amino acidpositions of SEQ ID NO: 2 according to the numbering of SEQ ID NO: 2,xvi) region 16 corresponding to amino acids 596 to 611 of SEQ ID NO: 2,said alteration being at one or more positions selected from the groupconsisting of positions: 596, 597, 598, 599, 600, 601, 602, 603, 604,605, 606, 607, 608, 609, 610, 611, wherein said positions correspond toamino acid positions of SEQ ID NO: 2 according to the numbering of SEQID NO: 2, xvii) region 17 corresponding to amino acids 661 to 805 of SEQID NO: 2, said alteration being at one or more positions selected fromthe group consisting of positions: 661, 662, 663, 664, 665, 666, 667,668, 669, 670, 671, 672, 673, 674, 675, 676, 677, 678, 679, 680, 681,682, 683, 684, 685, 686, 687, 688, 689, 690, 691, 692, 693, 694, 695,696, 697, 698, 699, 700, 701, 702, 703, 704, 705, 706, 707, 708, 709,710, 711, 712, 713, 714, 715, 716, 717, 718, 719, 720, 721, 722, 723,724, 725, 726, 727, 728, 729, 730, 731, 732, 733, 734, 735, 736, 737,738, 739, 740, 741, 742, 743, 744, 745, 746, 747, 748, 749, 750, 751,752, 753, 754, 755, 756, 757, 758, 759, 760, 761, 762, 763, 764, 765,766, 767, 768, 769, 770, 771, 772, 773, 774, 775, 776, 777, 778, 779,780, , 781, 782, 783, 784, 785, 786, 787, 788, 789, 790, 791, 792, 793,794, 795, 796, 797, 798, 799, 800, 801, 802, 803, 804, 805, wherein saidpositions correspond to amino acid positions of SEQ ID NO: 2 accordingto the numbering of SEQ ID NO: 2, xviii) region 18 corresponding toamino acids 829 to 838 to 1042 of SEQ ID NO: 2, said alteration being atone or more positions selected from the group consisting of positions:829, 830, 831, 832, 833, 834, 835, 836, 837, 838, wherein said positionscorrespond to amino acid positions of SEQ ID NO: 2 according to thenumbering of SEQ ID NO: 2, and xix) region 19 corresponding to aminoacids 1043 to 1055 of SEQ ID NO: 2, said alteration being at one or morepositions selected from the group consisting of positions 1043, 1044,1045, 1046, 1047, 1048, 1049, 1050, 1051, 1052, 1053, 1054, 1055,wherein said positions correspond to amino acid positions of SEQ ID NO:2 according to the numbering of SEQ ID NO:
 2. 3. The detergentcomposition according to claim 1, wherein said xanthan lyase variantcomprises an alteration at one or more positions in a region selectedfrom the group consisting of: i) region 1 corresponding to amino acids154 to 176 of SEQ ID NO: 6, said alteration being at one or morepositions selected from the group consisting of positions: 154, 155,156, 157, 158, 159, 160, 161, 162, 163, 164, 165, 166, 167, 168, 169,170, 171, 172, 173, 174, 175, 176, wherein said positions correspond toamino acid positions of SEQ ID NO: 6 according to the numbering of SEQID NO: 6, ii) region 2 corresponding to amino acids 614 to 658 of SEQ IDNO: 6, said alteration being at one or more positions selected from thegroup consisting of positions: 614, 615, 616, 617, 618, 619, 620, 621,622, 623, 624, 625, 626, 627, 628, 629, 630, 631, 632, 633, 634, 635,636, 637, 638, 639, 640, 641, 642, 643, 644, 645, 646, 647, 648, 649,650, 651, 652, 653, 654, 655, 656, 657, 658, wherein said positionscorrespond to amino acid positions of SEQ ID NO: 6 according to thenumbering of SEQ ID NO: 6, iii) region 3 corresponding to amino acids731 to 803 of SEQ ID NO: 6, said alteration being at one or morepositions selected from the group consisting of positions: 731, 732,733, 734, 735, 736, 737, 738, 739, 740, 741, 742, 743, 744, 745, 746,747, 748, 749, 750, 751, 752, 753, 754, 755, 756, 757, 758, 759, 760,761, 762, 763, 764, 765, 766, 767, 768, 769, 770, 771, 772, 773, 774,775, 776, 777, 778, 779, 780, , 781, 782, 783, 784, 785, 786, 787, 788,789, 790, 791, 792, 793, 794, 795, 796, 797, 798, 799, 800, 801, 802,803, wherein said positions correspond to amino acid positions of SEQ IDNO: 6 using according to the numbering of SEQ ID NO: 6, iv) region 4corresponding to amino acids 807 to 846 of SEQ ID NO: 6, said alterationbeing at one or more positions selected from the group consisting ofpositions: 807, 808, 809, 810, 811, 812, 813, 814, 815, 816, 817, 818,819, 820, 821, 822, 823, 824, 825, 826, 827, 828, 829, 830, 831, 832,833, 834, 835, 836, 837, 838, 839, 840, 841, 842, 843, 844, 845, 846,wherein said positions correspond to amino acid positions of SEQ ID NO:6 according to the numbering of SEQ ID NO: 6, v) region 5 correspondingto amino acids 872 to 885 of SEQ ID NO: 6, said alteration being at oneor more positions selected from the group consisting of positions: 872,873, 874, 875, 876, 877, 878, 879, 880, 881, 882, 883, 884, 885, whereinsaid positions correspond to amino acid positions of SEQ ID NO: 6according to the numbering of SEQ ID NO: 6, vi) region 6 correspondingto amino acids 903 to 1004 of SEQ ID NO: 6, said alteration being at oneor more positions selected from the group consisting of positions: 903,904, 905, 906, 907, 908, 909, 910, 911, 912, 913, 914, 915, 916, 917,918, 919, 920, 921, 922, 923, 924, 925, 926, 927, 928, 929, 930, 931,932, 933, 934, 935, 936, 937, 938, 939, 940, 941, 942, 943, 944, 945,946, 947, 948, 949, 950, 951, 952, 953, 954, 955, 956, 957, 958, 959,960, 961, 962, 963, 964, 965, 966, 967, 968, 969, 970, 971, 972, 973,974, 975, 976, 977, 978, 979, 980, 981, 982, 983, 984, 985, 986, 987,988, 989, 990, 991, 992, 993, 994, 995, 996, 997, 998, 999, 1000, 1001,1002, 1003, 1004, wherein said positions correspond to amino acidpositions of SEQ ID NO: 6 according to the numbering of SEQ ID NO:6[[)]], vii) region 7 corresponding to amino acids 1 to 153 of SEQ IDNO: 6, said alteration being at one or more positions selected from thegroup consisting of positions: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12,13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30,31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48,49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66,67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84,85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101,102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 113, 114, 115,116, 117, 118, 119, 120, 121, 122, 123, 124, 125, 126, 127, 128, 129,130, 131, 132, 133, 134, 135, 136, 137, 138, 139, 140, 141, 142, 143,144, 145, 146, 147, 148, 149, 150, 151, 152 and 153, wherein saidpositions correspond to amino acid positions of SEQ ID NO: 6 accordingto the numbering of SEQ ID NO:6, viii) region 8 corresponding to aminoacids 177 to 613 of SEQ ID NO: 6, said alteration being at one or morepositions selected from the group consisting of positions: 177, 178,179, 180, 181, 182, 183, 184, 185, 186, 187, 188, 189, 190, 191, 192,193, 194, 195, 196, 197, 198, 199, 200, 201, 202, 203, 204, 205, 206,207, 208, 209, 210, 211, 212, 213, 214, 215, 216, 217, 218, 219, 220,221, 222, 223, 224, 225, 226, 227, 228, 229, 230, 231, 232, 233, 234,235, 236, 237, 238, 239, 240, 241, 242, 243, 244, 245, 246, 247, 248,249, 250, 251, 252, 253, 254, 255, 256, 257, 258, 259, 260, 261, 262,263, 264, 265, 266, 267, 268, 269, 270, 271, 272, 273, 274, 275, 276,277, 278, 279, 280, 281, 282, 283, 284, 285, 286, 287, 288, 289, 290,291, 292, 293, 294, 295, 296, 297, 298, 299, 300, 301, 302, 303, 304,305, 306, 307, 308, 309, 310, 311, 312, 313, 314, 315, 316, 317, 318,319, 320, 321, 322, 323, 324, 325, 326, 327, 328, 329, 330, 331, 332,333, 334, 335, 336, 337, 338, 339, 340, 341, 342, 343, 344, 345, 346,347, 348, 349, 350, 351, 352, 353, 354, 355, 356, 357, 358, 359, 360,361, 362, 363, 364, 365, 366, 367, 368, 369, 370, 371, 372, 373, 374,375, 376, 377, 378, 379, 380, 381, 382, 383, 384, 385, 386, 387, 388,389, 390, 391, 392, 393, 394, 395, 396, 397, 398, 399, 400, 401, 402,403, 404, 405, 406, 407, 408, 409, 410, 411, 412, 413, 414, 415, 416,417, 418, 419, 420, 421, 422, 423, 424, 425, 426, 427, 428, 429, 430,431, 432, 433, 434, 435, 436, 437, 438, 439, 440, 441, 442, 443, 444,445, 446, 447, 448, 449, 450, 451, 452, 453, 454, 455, 456, 457, 458,459, 460, 461, 462, 463, 464, 465, 466, 467, 468, 469, 470, 471, 472,473, 474, 475, 476, 477, 478, 479, 480, 481, 482, 483, 484, 485, 486,487, 488, 489, 490, 491, 492, 493, 494, 495, 496, 497, 498, 499, 500,501, 502, 503, 504, 505, 506, 507, 508, 509, 510, 511, 512, 513, 514,515, 516, 517, 518, 519, 520, 521, 522, 523, 524, 525, 526, 527, 528,529, 530, 531, 532, 533, 534, 535, 536, 537, 538, 539, 540, 541, 542,543, 544, 545, 546, 547, 548, 549, 550, 551, 552, 553, 554, 555, 556,557, 558, 559, 560, 561, 562, 563, 564, 565, 566, 567, 568, 569, 570,571, 572, 573, 574, 575, 576, 577, 578, 579, 580, 581, 582, 583, 584,585, 586, 587, 588, 589, 590, 591, 592, 593, 594, 595, 596, 597, 598,599, 600, 601, 602, 603, 604, 605, 606, 607, 608, 609, 610, 611, 612 and613, wherein said positions correspond to amino acid positions of SEQ IDNO: 6 according to the numbering of SEQ ID NO:6, ix) region 9corresponding to amino acids 659 to 730 of SEQ ID NO: 6, said alterationbeing at one or more positions selected from the group consisting ofpositions: 659, 660, 661, 662, 663, 664, 665, 666, 667, 668, 669, 670,671, 672, 673, 674, 675, 676, 677, 678, 679, 680, 681, 682, 683, 684,685, 686, 687, 688, 689, 690, 691, 692, 693, 694, 695, 696, 697, 698,699, 700, 701, 702, 703, 704, 705, 706, 707, 708, 709, 710, 711, 712,713, 714, 715, 716, 717, 718, 719, 720, 721, 722, 723, 724, 725, 726,727, 728, 729 and 730, wherein said positions correspond to amino acidpositions of SEQ ID NO: 6 according to the numbering of SEQ ID NO:6, x)region 10 corresponding to amino acids 804 to 806 of SEQ ID NO: 6, saidalteration being at one or more positions selected from the groupconsisting of positions: 804, 805 and 806, wherein said positionscorrespond to amino acid positions of SEQ ID NO: 6 according to thenumbering of SEQ ID NO:6, xi) region 11 corresponding to amino acids 847to 871 of SEQ ID NO: 6, said alteration being at one or more positionsselected from the group consisting of positions: 847, 848, 849, 850,851, 852, 853, 854, 855, 856, 857, 858, 859, 860, 861, 862, 863, 864,865, 866, 867, 868, 869, 870 and 871, wherein said positions correspondto amino acid positions of SEQ ID NO: 6 according to the numbering ofSEQ ID NO:6, xii) region 12 corresponding to amino acids 886 to 902 ofSEQ ID NO: 6, said alteration being at one or more positions selectedfrom the group consisting of positions: 886, 887, 888, 889, 890, 891,892, 893, 894, 895, 896, 897, 898, 899, 900, 901 and 902, wherein saidpositions correspond to amino acid positions of SEQ ID NO: 6 accordingto the numbering of SEQ ID NO:6, and xiii) region 13 corresponding toamino acids 1005 to 1037 of SEQ ID NO: 6, said alteration being at oneor more positions selected from the group consisting of positions: 1005,1006, 1007, 1008, 1009, 1010, 1011, 1012, 1013, 1014, 1015, 1016, 1017,1018, 1019, 1020, 1021, 1022, 1023, 1024, 1025, 1026, 1027, 1028, 1029,1030, 1031, 1032, 1033, 1034, 1035, 1036 and 1037, wherein saidpositions correspond to amino acid positions of SEQ ID NO: 6 accordingto the numbering of SEQ ID NO:6.
 4. The detergent composition accordingto claim 1, wherein the endoglucanase variant has an alteration in thepositions selected from the group consisting of positions: 559+579+697;512+559+579+697; 18+71+186+408+579+602+651+688+756;18+189+408+559+579+688+697+756+921+934; 313+488; 880+905+921+934;302+313+408+579+602+651+697+880+921+934; or216+313+408+476+579+602+638+651+697+719+880+887+921+934 of SEQ ID NO: 2.5. The detergent composition according to claim 1, wherein the xanthanlyase variant has an alteration in the positions selected from the groupconsisting of positions: 190, 229, 234, 440, 582, 624, 631, 635, 672,703, 738, 752, 753, 754, 757, 769, 775, 801, 875, 892, and anycombination thereof.
 6. The detergent composition according to claim 1,wherein the detergent composition comprises: (A) an endoglucanasevariant selected from those having at least 61%, sequence identity toSEQ ID NO: 2 and having an alteration, in the positions selected fromthe group consisting of positions: 559+579+697; 512+559+579+697;18+71+186+408+579+602+651+688+756;18+189+408+559+579+688+697+756+921+934; 313+488; 880+905+921+934;302+313+408+579+602+651+697+880+921+934; or216+313+408+476+579+602+638+651+697+719+880+887+921+934 of SEQ ID NO: 2;and (B) a xanthan lyase variant having at least 61%, sequence identityto SEQ ID NO: 6 and having an alteration, in the positions selected fromthe group consisting of positions: 229+672+752+753+769+775+801+875+892;229+672+753+754+769+775+801+875+892;229+672+752+753+754+769+775+801+875+892;190+229+234+624+672+753+754+769+775+801+875;190+229+631+672+703+752+753+769+775+801+875;190+229+234+582+672+753+754+757+769+775+801+875+892;229+440+582+624+635+672+738+753+754+757+769+775+801+875+892; or100+229+360+458+582+672+753+754+757+769+775+801+843+875+892+1008 of SEQID NO: 6; and (B 8)S100D+E229S+K360G+D458S+S582K+N672D+G753E+S754E+S757D+A769D+L775A+D801G+A843P+K875T+N892Y+N1008D of SEQ ID NO:
 6. 7. The detergentcomposition according to claim 6, wherein the endoglucanase and/or thexanthan lyase variant do not comprise any further substitution.
 8. Thedetergent composition according to claim 1, wherein the detergentcomposition additionally comprises one or more further detergentcomponents.
 9. (canceled)
 10. A method of degrading xanthan gum bycontacting the xanthan gum with a detergent composition according toclaim 1, wherein the xanthan gum is disposed on a surface of a hardsurface or textile.